PLXNB3 (NM_001163257) Human Tagged ORF Clone

CAT#: RC228700

PLXNB3 (Myc-DDK-tagged)-Human plexin B3 (PLXNB3), transcript variant 2

Product Images

Circular map for RC228700

• 

  Western blot validation of overexpression lysate (Cat# LY431728) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228700 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLXNB3
• Synonyms: PLEXB3; PLEXR; PLXN6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228700 representing NM_001163257
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCACCCCTGCCTCTTCGCTGACTTGCTCCTTGCTCAGCCCGCGGCTGCCTGGCTCTTTCCCCC
  AGCTGCGGAGGGTTCCTCCTTGCAGCCGGCCCTGGCTGCCCAAGGCCCCCGTGATGGCTCGCTGGCCTCC
  CTTCGGCCTCTGCCTCCTCCTGCTGCTGCTGTCCCCACCGCCACTGCCCTTGACAGGGGCCCATCGCTTC
  TCCGCACCTAATACCACTCTCAACCACTTGGCACTGGCACCTGGCCGAGGCACACTCTATGTCGGCGCAG
  TGAACCGCCTCTTCCAGCTCAGCCCCGAGCTGCAGCTCGAGGCCGTGGCTGTCACTGGCCCTGTAATCGA
  CAGCCCTGACTGCGTGCCCTTCCGTGACCCAGCCGAGTGCCCACAGGCCCAGCTCACTGACAATGCCAAC
  CAGCTGCTGCTGGTGAGCAGCCGCGCCCAGGAGCTGGTGGCCTGCGGGCAGGTGCGGCAGGGCGTGTGTG
  AGACACGGCGCCTTGGGGATGTGGCCGAGGTGCTGTACCAGGCTGAGGACCCTGGTGACGGGCAGTTTGT
  GGCTGCCAATACCCCGGGAGTGGCAACGGTGGGGCTGGTGGTGCCCTTGCCCGGCCGGGACCTCCTGCTT
  GTGGCCAGAGGCCTGGCGGGCAAGCTGTCGGCAGGGGTGCCACCCCTGGCCATCCGCCAGCTGGCCGGGT
  CTCAGCCCTTCTCCAGCGAGGGCCTGGGCCGCCTGGTGGTGGGCGACTTCTCCGACTACAACAACAGCTA
  CGTCGGGGCCTTTGCCGACGCCCGCTCCGCCTACTTCGTGTTCCGCCGCCGCGGGGCCCGGGCCCAGGCT
  GAGTACCGCTCCTACGTGGCCCGCGTCTGCCTGGGGGACACCAACCTGTACTCCTACGTGGAGGTCCCCC
  TCGCCTGCCAGGGCCAGGGCCTCATCCAGGCCGCCTTCCTTGCCCCGGGCACCTTGCTAGGGGTGTTTGC
  CGCGGGCCCAAGGGGCACCCAGGCGGCGCTCTGTGCCTTCCCCATGGTGGAGCTGGGTGCCAGCATGGAG
  CAGGCCCGGAGACTCTGCTACACGGCGGGCGGCCGGGGCCCCAGCGGCGCAGAGGAAGCCACCGTGGAGT
  ACGGCGTCACGTCGCGCTGCGTCACCCTGCCCCTTGATTCCCCCGAGTCGTACCCCTGTGGCGACGAGCA
  CACCCCCAGCCCCATTGCTGGCCGCCAGCCCCTGGAGGTCCAGCCTCTGCTGAAGCTCGGGCAGCCGGTC
  AGCGCCGTGGCAGCTCTCCAGGCAGATGGGCACATGATAGCCTTCCTGGGGGACACCCAGGGCCAGCTGT
  ACAAGGTCTTTCTCCACGGCTCCCAGGGCCAGGTTTACCACTCCCAGCAAGTGGGGCCTCCAGGCTCAGC
  CATCAGCCCAGACCTGCTGCTGGACAGCAGTGGCAGTCACCTCTATGTCCTGACTGCCCACCAGGTGGAC
  CGGATACCTGTGGCAGCCTGCCCCCAGTTCCCTGACTGTGCCAGCTGCCTCCAGGCCCAGGACCCGCTGT
  GTGGCTGGTGTGTCCTCCAGGGCAGGTGTACCCGGAAGGGCCAGTGCGGGCGGGCAGGCCAGCTGAACCA
  GTGGCTGTGGAGTTATGAGGAGGACAGCCACTGCCTGCACATCCAGAGCCTGCTGCCGGGCCACCACCCC
  CGCCAGGAGCAGGGCCAGGTCACTTTGTCTGTCCCCCGGCTGCCCATCCTGGATGCAGATGAATACTTCC
  ATTGTGCGTTCGGGGACTATGACAGCTTGGCTCATGTGGAAGGGCCCCACGTGGCCTGTGTCACCCCTCC
  CCAAGACCAGGTGCCACTTAACCCTCCAGGCACAGACCACGTCACTGTGCCCCTGGCCCTGATGTTCGAG
  GACGTGACTGTGGCTGCCACCAACTTCTCCTTTTATGACTGCAGTGCCGTCCAGGCCTTGGAGGCGGCTG
  CCCCGTGTCGCGCTTGCGTGGGCAGCATCTGGCGGTGTCACTGGTGCCCGCAGAGTAGCCACTGCGTGTA
  CGGAGAGCACTGCCCAGAGGGCGAGAGGACCATCTACAGCGCCCAGGAGGTGGACATCCAGGTGCGTGGC
  CCAGGGGCTTGCCCACAGGTCGAAGGCCTGGCAGGTCCCCACCTGGTGCCTGTGGGCTGGGAGAGCCATT
  TGGCCCTACGCGTGCGGAACCTTCAACATTTCCGAGGCCTGCCTGCCTCCTTCCACTGCTGGCTGGAGCT
  GCCTGGAGAACTTCGGGGACTGCCGGCCACCCTGGAGGAGACAGCAGGGGATTCAGGCCTCATCCACTGC
  CAGGCCCACCAGTTTTATCCCTCCATGTCCCAGCGGGAGCTCCCAGTGCCCATCTACGTCACCCAGGGTG
  AAGCCCAGAGGCTGGACAACACCCATGCTCTTTATGTGATCCTGTACGACTGCGCCATGGGCCACCCGGA
  CTGCAGCCACTGCCAAGCGGCCAACAGGAGCCTGGGCTGCCTGTGGTGTGCTGACGGCCAGCCTGCCTGT
  CGCTATGGGCCCTTGTGCCCGCCGGGGGCTGTGGAGCTGCTGTGTCCTGCGCCCAGCATTGATGCAGTCG
  AGCCCCTGACCGGTCCCCCTGAGGGAGGCTTGGCCCTCACCATCCTGGGCTCCAACCTGGGCCGGGCCTT
  CGCCGATGTGCAGTACGCCGTGAGCGTGGCCAGCCGGCCCTGCAACCCTGAGCCCTCTCTCTACCGCACG
  TCGGCCCGGATTGTGTGTGTGACATCTCCTGCCCCCAATGGCACCACTGGGCCCGTCCGGGTGGCCATTA
  AGAGCCAGCCACCAGGCATCTCAAGCCAGCACTTCACCTACCAGGACCCTGTCCTGCTGAGCCTGAGTCC
  TCGCTGGGGCCCCCAGGCAGGGGGCACCCAGCTCACCATCCGAGGTCAGCACCTCCAGACAGGTGGCAAC
  ACCAGTGCCTTCGTGGGTGGCCAACCCTGTCCCATCCTGGAGCCAGTGTGTCCGGAGGCCATCGTGTGCC
  GTACCAGGCCCCAGGCTGCCCCAGGAGAAGCAGCGGTCCTTGTGGTCTTTGGCCATGCCCAGCGCACACT
  GCTCGCCAGCCCCTTCCGCTACACCGCCAACCCCCAGCTTGTAGCGGCGGAGCCCAGTGCCAGCTTCCGG
  GGGGGTGGGCGACTGATCCGTGTCAGGGGCACCGGCCTAGACGTGGTGCAGCGGCCCCTACTGTCTGTGT
  GGCTGGAGGCTGACGCAGAGGTGCAGGCTTCCAGGGCCCAGCCCCAGGACCCACAGCCAAGGAGGAGCTG
  TGGAGCCCCTGCTGCGGACCCCCAGGCTTGTATCCAGCTCGGTGGGGGGCTGCTGCAGTGCTCCACCGTC
  TGCTCCGTCAACTCGTCCAGCCTCCTCCTGTGCCGGAGCCCTGCTGTACCAGACAGAGCCCACCCGCAGC
  GGGTCTTCTTCACCCTAGACAACGTGCAAGTGGACTTCGCCAGTGCCAGTGGGGGCCAGGGCTTCCTGTA
  CCAGCCCAACCCCCGCCTGGCACCCCTCAGCCGCGAGGGGCCTGCCCGCCCCTACCGCCTCAAGCCAGGC
  CATGTCCTGGATGTGGAGGGCGAGGGCCTCAACCTGGGCATCAGCAAGGAGGAGGTGCGCGTGCACATCG
  GCCGCGGCGAGTGCCTGGTGAAGACGCTCACGCGCACCCACCTGTACTGCGAGCCGCCTGCGCACGCCCC
  GCAGCCTGCCAATGGCTCCGGCCTGCCACAGTTCGTGGTGCAGATGGGCAATGTGCAGCTGGCCCTGGGC
  CCTGTGCAGTACGAGGCTGAACCCCCGCTGTCTGCCTTTCCCGTGGAGGCCCAGGCAGGCGTGGGCATGG
  GTGCTGCAGTGCTGATTGCCGCCGTGCTCCTCCTCACCCTCATGTACAGGCACAAGAGCAAGCAGGCCCT
  GCGGGACTACCAGAAGGTGCTAGTGCAGCTGGAGAGCCTGGAGACCGGCGTGGGAGACCAGTGCCGCAAG
  GAGTTCACAGACCTCATGACGGAGATGACCGACCTCAGCAGCGACCTGGAGGGCAGCGGGATCCCCTTCC
  TGGACTACCGCACCTACGCCGAGCGCGCCTTCTTCCCTGGCCATGGCGGTTGCCCGCTGCAGCCCAAGCC
  TGAGGGGCCAGGGGAGGACGGCCACTGTGCCACTGTGCGCCAGGGCCTCACGCAGCTCTCCAACCTGCTC
  AACAGCAAGCTCTTCCTCCTCACGCTCATCCACACCCTGGAGGAGCAGCCCAGCTTTTCCCAGAGGGATC
  GCTGCCATGTGGCTTCGCTGCTGTCGCTAGCGCTACACGGCAAGCTGGAGTACCTGACGGACATCATGAG
  GACCCTGCTGGGTGACCTGGCGGCCCATTACGTGCACAGGAACCCCAAGCTCATGCTACGCAGGACAGAG
  ACCATGGTGGAGAAACTGCTCACCAACTGGCTGTCCATCTGCCTGTACGCCTTCCTGAGGGAGGTGGCTG
  GTGAACCACTGTACATGCTCTTCCGGGCCATCCAGTACCAGGTGGACAAAGGCCCCGTGGACGCCGTGAC
  AGGCAAGGCCAAACGGACCCTGAATGATAGCCGCTTGCTGCGGGAGGACGTGGAGTTCCAGCCCCTGACG
  CTGATGGTGCTGGTGGGGCCCGGGGCTGGCGGGGCCGCAGGCAGCAGCGAGATGCAGCGCGTGCCAGCCC
  GGGTGCTCGACACGGACACCATCACCCAGGTCAAGGAGAAGGTGTTGGACCAAGTCTACAAGGGCACCCC
  CTTCTCCCAGAGGCCCTCAGTGCATGCCCTAGACCTTGAGTGGCGCTCAGGCCTGGCTGGTCACCTGACC
  CTATCGGACGAAGACTTGACCTCCGTGACCCAAAACCACTGGAAGAGACTCAACACCTTGCAACACTACA
  AGGTCCCAGATGGAGCAACAGTGGGGCTCGTCCCTCAGCTGCACCGTGGCAGCACCATCTCCCAGAGCCT
  GGCCCAGAGGTGCCCCTTGGGAGAGAACATACCCACGCTGGAGGATGGCGAGGAGGGGGGGGTGTGCCTC
  TGGCACCTGGTGAAAGCCACCGAGGAGCCAGAAGGGGCCAAGGTGCGGTGCAGCAGCCTGCGGGAGCGCG
  AGCCAGCAAGGGCCAAGGCCATTCCGGAAATCTACCTCACCCGTCTGCTGTCCATGAAGGGCACGCTGCA
  GAAGTTTGTGGACGACACCTTCCAGGCCATTCTCAGCGTGAACCGGCCCATCCCCATCGCCGTCAAGTAC
  CTGTTTGACCTTCTGGATGAGCTAGCAGAGAAGCACGGCATCGAGGACCCAGGGACCCTGCACATCTGGA
  AGACCAACAGTCTGCTGCTGCGGTTCTGGGTGAATGCCTTGAAGAACCCACAGCTCATCTTTGATGTACG
  GGTGTCGGACAATGTGGACGCCATCCTTGCTGTCATCGCCCAGACCTTCATTGACTCCTGTACCACCTCG
  GAGCATAAAGTGGGCCGGGATTCCCCAGTGAACAAACTGCTCTACGCCCGGGAGATCCCACGCTACAAGC
  AGATGGTGGAGAGGTACTATGCGGACATTCGCCAGAGCTCTCCGGCGAGCTACCAGGAGATGAACTCTGC
  TTTGGCTGAGCTCTCCGGGAACTACACTTCTGCTCCCCACTGTCTGGAGGCTCTGCAAGAACTCTACAAC
  CACATCCACAGGTACTATGATCAGATTATCAGTGCCCTGGAGGAGGACCCTGTGGGCCAGAAGCTGCAGC
  TGGCCTGCCGCCTGCAGCAGGTCGCCGCCCTGGTGGAAAACAAAGTGACTGACCTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228700 representing NM_001163257
  Red=Cloning site Green=Tags(s)
  
  MELTPASSLTCSLLSPRLPGSFPQLRRVPPCSRPWLPKAPVMARWPPFGLCLLLLLLSPPPLPLTGAHRF
  SAPNTTLNHLALAPGRGTLYVGAVNRLFQLSPELQLEAVAVTGPVIDSPDCVPFRDPAECPQAQLTDNAN
  QLLLVSSRAQELVACGQVRQGVCETRRLGDVAEVLYQAEDPGDGQFVAANTPGVATVGLVVPLPGRDLLL
  VARGLAGKLSAGVPPLAIRQLAGSQPFSSEGLGRLVVGDFSDYNNSYVGAFADARSAYFVFRRRGARAQA
  EYRSYVARVCLGDTNLYSYVEVPLACQGQGLIQAAFLAPGTLLGVFAAGPRGTQAALCAFPMVELGASME
  QARRLCYTAGGRGPSGAEEATVEYGVTSRCVTLPLDSPESYPCGDEHTPSPIAGRQPLEVQPLLKLGQPV
  SAVAALQADGHMIAFLGDTQGQLYKVFLHGSQGQVYHSQQVGPPGSAISPDLLLDSSGSHLYVLTAHQVD
  RIPVAACPQFPDCASCLQAQDPLCGWCVLQGRCTRKGQCGRAGQLNQWLWSYEEDSHCLHIQSLLPGHHP
  RQEQGQVTLSVPRLPILDADEYFHCAFGDYDSLAHVEGPHVACVTPPQDQVPLNPPGTDHVTVPLALMFE
  DVTVAATNFSFYDCSAVQALEAAAPCRACVGSIWRCHWCPQSSHCVYGEHCPEGERTIYSAQEVDIQVRG
  PGACPQVEGLAGPHLVPVGWESHLALRVRNLQHFRGLPASFHCWLELPGELRGLPATLEETAGDSGLIHC
  QAHQFYPSMSQRELPVPIYVTQGEAQRLDNTHALYVILYDCAMGHPDCSHCQAANRSLGCLWCADGQPAC
  RYGPLCPPGAVELLCPAPSIDAVEPLTGPPEGGLALTILGSNLGRAFADVQYAVSVASRPCNPEPSLYRT
  SARIVCVTSPAPNGTTGPVRVAIKSQPPGISSQHFTYQDPVLLSLSPRWGPQAGGTQLTIRGQHLQTGGN
  TSAFVGGQPCPILEPVCPEAIVCRTRPQAAPGEAAVLVVFGHAQRTLLASPFRYTANPQLVAAEPSASFR
  GGGRLIRVRGTGLDVVQRPLLSVWLEADAEVQASRAQPQDPQPRRSCGAPAADPQACIQLGGGLLQCSTV
  CSVNSSSLLLCRSPAVPDRAHPQRVFFTLDNVQVDFASASGGQGFLYQPNPRLAPLSREGPARPYRLKPG
  HVLDVEGEGLNLGISKEEVRVHIGRGECLVKTLTRTHLYCEPPAHAPQPANGSGLPQFVVQMGNVQLALG
  PVQYEAEPPLSAFPVEAQAGVGMGAAVLIAAVLLLTLMYRHKSKQALRDYQKVLVQLESLETGVGDQCRK
  EFTDLMTEMTDLSSDLEGSGIPFLDYRTYAERAFFPGHGGCPLQPKPEGPGEDGHCATVRQGLTQLSNLL
  NSKLFLLTLIHTLEEQPSFSQRDRCHVASLLSLALHGKLEYLTDIMRTLLGDLAAHYVHRNPKLMLRRTE
  TMVEKLLTNWLSICLYAFLREVAGEPLYMLFRAIQYQVDKGPVDAVTGKAKRTLNDSRLLREDVEFQPLT
  LMVLVGPGAGGAAGSSEMQRVPARVLDTDTITQVKEKVLDQVYKGTPFSQRPSVHALDLEWRSGLAGHLT
  LSDEDLTSVTQNHWKRLNTLQHYKVPDGATVGLVPQLHRGSTISQSLAQRCPLGENIPTLEDGEEGGVCL
  WHLVKATEEPEGAKVRCSSLREREPARAKAIPEIYLTRLLSMKGTLQKFVDDTFQAILSVNRPIPIAVKY
  LFDLLDELAEKHGIEDPGTLHIWKTNSLLLRFWVNALKNPQLIFDVRVSDNVDAILAVIAQTFIDSCTTS
  EHKVGRDSPVNKLLYAREIPRYKQMVERYYADIRQSSPASYQEMNSALAELSGNYTSAPHCLEALQELYN
  HIHRYYDQIISALEEDPVGQKLQLACRLQQVAALVENKVTDL
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163257
• ORF Size: 5796 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163257.1, NP_001156729.1
• RefSeq ORF: 5799 bp
• Locus ID: 5365
• UniProt ID: Q9ULL4
• Cytogenetics: Xq28
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance
• MW: 209.1 kDa
• Gene Summary: The protein encoded by this gene is a member of the plexin family. It functions as a receptor for semaphorin 5A, and plays a role in axon guidance, invasive growth and cell migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]