SAPS3 (PPP6R3) (NM_001164160) Human Tagged ORF Clone

CAT#: RC228606

PPP6R3 (Myc-DDK-tagged)-Human protein phosphatase 6, regulatory subunit 3 (PPP6R3), transcript variant 4

Product Images

Circular map for RC228606

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  Western blot validation of overexpression lysate (Cat# LY431634) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228606 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PPP6R3 protein (Cat# TP328606). The protein was produced from HEK293T cells transfected with PPP6R3 cDNA clone (Cat# RC228606) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SAPS3
• Synonyms: C11orf23; PP6R3; SAP190; SAPL; SAPLa; SAPS3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228606 representing NM_001164160
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTTGGAAATTTGATCTTCACTCATCATCCCACATAGACACACTTCTAGAAAGAGAAGATGTAACAC
  TGAAGGAGTTAATGGATGAGGAAGATGTTTTACAGGAATGTAAAGCTCAGAACCGCAAACTTATAGAGTT
  TCTGTTAAAAGCAGAATGTCTCGAAGATTTAGTCTCATTCATTATAGAAGAACCACCTCAAGACATGGAT
  GAAAAGATCAGATACAAGTATCCAAATATATCTTGTGAGTTGCTCACTTCTGATGTCTCCCAGATGAATG
  ATAGACTGGGAGAAGATGAATCCTTGCTAATGAAATTATATAGCTTCCTCCTAAACGATTCCCCTTTGAA
  TCCACTACTTGCCAGTTTCTTCAGCAAGGTGCTAAGTATTCTTATCAGCAGAAAACCAGAACAGATTGTG
  GATTTCTTAAAGAAGAAGCATGATTTTGTAGACCTTATTATAAAGCACATAGGAACTTCTGCTATCATGG
  ATTTGTTGCTCAGGCTCCTGACGTGTATCGAACCTCCACAGCCCAGGCAAGATGTGCTGAATTGGTTAAA
  TGAGGAGAAAATTATCCAGAGGCTTGTGGAAATAGTTCATCCATCGCAAGAAGAAGATCGACATTCAAAT
  GCATCACAATCACTTTGTGAAATTGTTCGCCTGAGCAGAGACCAGATGTTACAAATTCAGAACAGTACAG
  AGCCCGACCCCCTGCTTGCCACTCTAGAAAAGCAAGAAATTATAGAGCAGCTTCTATCAAATATTTTCCA
  CAAGGAGAAAAATGAGTCAGCCATAGTCAGTGCAATCCAGATATTGCTGACTTTACTTGAGACACGACGA
  CCAACATTTGAAGGCCATATAGAGATCTGCCCACCAGGCATGAGCCATTCAGCTTGTTCAGTAAACAAGA
  GTGTTCTAGAAGCCATCAGAGGAAGACTTGGATCTTTTCATGAACTCCTGCTGGAGCCACCCAAGAAAAG
  TGTGATGAAGACCACATGGGGTGTGCTGGATCCTCCTGTGGGGAATACCCGGTTGAATGTCATTAGGTTG
  ATATCCAGCCTGCTTCAAACCAATACCAGCAGTATAAATGGGGACCTTATGGAGCTGAATAGCATTGGAG
  TCATATTGAACATGTTCTTCAAGTATACATGGAATAACTTTTTGCATACACAAGTGGAAATTTGTATTGC
  ACTGATTCTTGCAAGTCCTTTTGAAAACACAGAAAATGCCACAATTACCGATCAAGACTCCACTGGTGAT
  AATTTGTTATTAAAACATCTTTTCCAAAAATGTCAATTAATAGAACGAATACTTGAAGCCTGGGAAATGA
  ATGAGAAGAAACAGGCTGAGGGAGGAAGACGGCATGGTTACATGGGACACCTAACGAGGATAGCTAACTG
  TATCGTGCACAGCACTGACAAGGGCCCCAACAGTGCATTAGTGCAGCAGCTTATCAAAGATCTTCCCGAC
  GAAGTCAGGGAACGATGGGAGACGTTCTGCACAAGCTCCTTAGGAGAAACTAACAAGAGGAACACGGTAG
  ATCTAGTTACAACCTGCCATATTCATTCATCCAGTGATGATGAAATTGACTTTAAAGAAACGGGTTTCTC
  ACAGGATTCTTCTTTGCAGCAAGCCTTTTCTGATTATCAGATGCAACAAATGACGTCCAATTTTATTGAC
  CAGTTTGGCTTCAACGATGAGAAGTTTGCAGATCAAGATGACATTGGCAATGTTTCTTTTGATCGAGTAT
  CAGACATCAACTTTACTCTCAATACAAATGAAAGTGGAAATATTGCCTTGTTTGAAGCATGTTGTAAGGA
  AAGAATACAACAGTTTGATGATGGTGGCTCTGATGAGGAAGATATATGGGAGGAAAAGCACATCGCATTC
  ACACCAGAATCCCAAAGACGATCCAGCTCGGGGAGTACAGACAGTGAGGAAAGTACAGACTCTGAAGAAG
  AAGATGGAGCAAAGCAAGACTTGTTTGAACCCAGCAGTGCCAACACGGAGGATAAAATGGAGGTGGACCT
  GAGTGAACCACCCAACTGGTCAGCTAACTTTGATGTCCCAATGGAAACAACCCACGGTGCTCCATTGGAT
  TCTGTGGGATCTGATGTCTGGAGCACAGAGGAGCCGATGCCAACTAAAGAGACGGGCTGGGCTTCTTTTT
  CAGAGTTCACGTCTTCCCTGAGCACAAAAGATTCTTTAAGGAGTAATTCTCCAGTGGAAATGGAAACCAG
  CACTGAACCCATGGACCCTCTGACTCCCAGTGCGGCTGCCCTGGCAGTGCAGCCAGAAGCGGCAGGCAGT
  GTGGCCATGGAAGCCAGCTCTGACGGAGAGGAGGATGCAGAAAGTACAGACAAGGTAACTGAGACAGTGA
  TGAATGGCGGCATGAAGGAAACGCTCAGCCTCACTGTAGATGCCAAGACAGAGACTGCGGTCTTCAAAAG
  AGTGTTGAAATCCTATCGTGAGGAAGGGAAACTGTCTACCTCTCAAGATGCTGCTTGTAAAGACGCAGAG
  GAGTGTCCCGAGACTGCAGAGGCGAAGTGCGCGGCGCCCAGGCCTCCCAGCAGCAGTCCCGAGCAGAGGA
  CTGGCCAACCAAGCGCACCAGGTGACACTTCAGTGAATGGCCCTGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228606 representing NM_001164160
  Red=Cloning site Green=Tags(s)
  
  MFWKFDLHSSSHIDTLLEREDVTLKELMDEEDVLQECKAQNRKLIEFLLKAECLEDLVSFIIEEPPQDMD
  EKIRYKYPNISCELLTSDVSQMNDRLGEDESLLMKLYSFLLNDSPLNPLLASFFSKVLSILISRKPEQIV
  DFLKKKHDFVDLIIKHIGTSAIMDLLLRLLTCIEPPQPRQDVLNWLNEEKIIQRLVEIVHPSQEEDRHSN
  ASQSLCEIVRLSRDQMLQIQNSTEPDPLLATLEKQEIIEQLLSNIFHKEKNESAIVSAIQILLTLLETRR
  PTFEGHIEICPPGMSHSACSVNKSVLEAIRGRLGSFHELLLEPPKKSVMKTTWGVLDPPVGNTRLNVIRL
  ISSLLQTNTSSINGDLMELNSIGVILNMFFKYTWNNFLHTQVEICIALILASPFENTENATITDQDSTGD
  NLLLKHLFQKCQLIERILEAWEMNEKKQAEGGRRHGYMGHLTRIANCIVHSTDKGPNSALVQQLIKDLPD
  EVRERWETFCTSSLGETNKRNTVDLVTTCHIHSSSDDEIDFKETGFSQDSSLQQAFSDYQMQQMTSNFID
  QFGFNDEKFADQDDIGNVSFDRVSDINFTLNTNESGNIALFEACCKERIQQFDDGGSDEEDIWEEKHIAF
  TPESQRRSSSGSTDSEESTDSEEEDGAKQDLFEPSSANTEDKMEVDLSEPPNWSANFDVPMETTHGAPLD
  SVGSDVWSTEEPMPTKETGWASFSEFTSSLSTKDSLRSNSPVEMETSTEPMDPLTPSAAALAVQPEAAGS
  VAMEASSDGEEDAESTDKVTETVMNGGMKETLSLTVDAKTETAVFKRVLKSYREEGKLSTSQDAACKDAE
  ECPETAEAKCAAPRPPSSSPEQRTGQPSAPGDTSVNGPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164160
• ORF Size: 2637 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164160.2
• RefSeq ORF: 2640 bp
• Locus ID: 55291
• UniProt ID: Q5H9R7
• Cytogenetics: 11q13.2
• MW: 98.3 kDa
• Gene Summary: Protein phosphatase regulatory subunits, such as SAPS3, modulate the activity of protein phosphatase catalytic subunits by restricting substrate specificity, recruiting substrates, and determining the intracellular localization of the holoenzyme. SAPS3 is a regulatory subunit for the protein phosphatase-6 catalytic subunit (PPP6C; MIM 612725) (Stefansson and Brautigan, 2006 [PubMed 16769727]).[supplied by OMIM, Nov 2010]