C1orf185 (NM_001136508) Human Tagged ORF Clone

CAT#: RC227961

C1orf185 (Myc-DDK-tagged)-Human chromosome 1 open reading frame 185 (C1orf185)

Product Images

Circular map for RC227961

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  Western blot validation of overexpression lysate (Cat# LY427905) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227961 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C1orf185
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227961 representing NM_001136508
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCACCTAAAGGTTTTTTTAATTACTTGACCTATTTTCTTGCTGCTGGTGCTGTCACTTTGGGAA
  TTGGTTTCTTTGCTTTGGCATCAGCTTTGTGGTTCCTGATTTGCAAACGAAGAGAAATATTTCAAAATTC
  CAAATTTAAAGCAATTGATGAGAGATGCAGGCAAAGACCATCAATGGCGAAGATTAAATCTCATTCTCAG
  TGTGTTTTTATTTCTCGAAATTTTCATACTGGGAGATTCCAATTACAGGAGGAGCAAAGAAAAAAGGAAG
  CAGCACATATAAAAGCAATTAAAGATCATTCTAAAGATGAACCCCAACTTGCAACAAAAAATATCATTTG
  TGATCCCTCAGAGACCAGCTCCACAACAAATCGCAGCAGTGTTACATTAAGCTTATCAACATTACCATCT
  GATTCTTATTACAGCCAAAGTATAGAAGCAGCTGATGACTGGTTTTCTGATGATTCTCTAGTGAAAAGGA
  ACTCTCCAATGCCTTCTCTCGGGGAACCTCTAATGGAAAAAGTATTTTCATACCTGTCAACCATTTCATT
  AGAAGAGGGTACTGAAAGTGTACTGAATGACACTTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227961 representing NM_001136508
  Red=Cloning site Green=Tags(s)
  
  MASPKGFFNYLTYFLAAGAVTLGIGFFALASALWFLICKRREIFQNSKFKAIDERCRQRPSMAKIKSHSQ
  CVFISRNFHTGRFQLQEEQRKKEAAHIKAIKDHSKDEPQLATKNIICDPSETSSTTNRSSVTLSLSTLPS
  DSYYSQSIEAADDWFSDDSLVKRNSPMPSLGEPLMEKVFSYLSTISLEEGTESVLNDTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001136508
• ORF Size: 597 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136508.2
• RefSeq ORF: 600 bp
• Locus ID: 284546
• UniProt ID: Q5T7R7
• Cytogenetics: 1p32.3
• Protein Families: Transmembrane
• MW: 22.2 kDa