MTFR1 (NM_001145838) Human Tagged ORF Clone

CAT#: RC227926

MTFR1 (Myc-DDK-tagged)-Human mitochondrial fission regulator 1 (MTFR1), nuclear gene encoding mitochondrial protein, transcript variant 2

Product Images

Circular map for RC227926

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  Western blot validation of overexpression lysate (Cat# LY429023) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227926 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MTFR1 protein (Cat# TP327926). The protein was produced from HEK293T cells transfected with MTFR1 cDNA clone (Cat# RC227926) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MTFR1
• Synonyms: CHPPR; FAM54A2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227926 representing NM_001145838
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTGGCTGGATTAAGCGCCTAATTAGGATGGTTTTTCAACAAGTTGGAGTAAGCATGCAATCGATTA
  ACAGCCATGCAACAGAATGGAGTCCCAGCCACCCAGGAGAGGATGCAGTGGCGTCTTTTGCTGATGTTGG
  ATGGGTAGCCAAAGAAGAAGGAGAGTGTTCAGCAAGACTAAGGACAGAGGTCAGATCAAGGCCACCCCTT
  CAGGATGACCTTCTTTTCTTTGAGAAGGCCCCAAGCAGACAGATTTCCTTACCAGACTTGTCTCAAGAAG
  AGCCTCAGCTGAAGACCCCAGCGCTGGCAAATGAGGAAGCACTGCAGAAGATTTGCGCTCTCGAAAATGA
  ACTTGCTGCTCTCAGAGCTCAGATTGCCAAAATTGTGACCCAGCAGGAGCAGCAAAATCTCACTGCAGGT
  GACTTAGATTCTACCACATTTGGTACCATACCACCACACCCTCCACCTCCCCCACCGCCCCTGCCTCCCC
  CTGCACTGGGGCTCCACCAAAGTACATCTGCTGTTGATCTGATTAAAGAACGAAGAGAGAAAAGAGCCAA
  TGCTGGAAAGACTTTGGTTAAGAACAATCCAAAGAAACCTGAAATGCCAAATATGCTAGAGATCCTTAAA
  GAGATGAACAGTGTAAAACTTCGGTCAGTGAAGAGGTCAGAGCAAGATGTGAAGCCCAAGCCAGTGGATG
  CTACTGACCCTGCTGCCCTCATAGCAGAGGCTCTGAAAAAGAAATTTGCTTATCGGTATCGAAGTGATAG
  CCAAGATGAAGTTGAAAAAGGAATTCCAAAGTCTGAATCAGAGGCCACCTCAGAGAGAGTGTTGTTTGGG
  CCACACATGTTGAAGCCAACAGGAAAAATGAAGGCTTTAATTGAAAATGTATCAGACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227926 representing NM_001145838
  Red=Cloning site Green=Tags(s)
  
  MLGWIKRLIRMVFQQVGVSMQSINSHATEWSPSHPGEDAVASFADVGWVAKEEGECSARLRTEVRSRPPL
  QDDLLFFEKAPSRQISLPDLSQEEPQLKTPALANEEALQKICALENELAALRAQIAKIVTQQEQQNLTAG
  DLDSTTFGTIPPHPPPPPPPLPPPALGLHQSTSAVDLIKERREKRANAGKTLVKNNPKKPEMPNMLEILK
  EMNSVKLRSVKRSEQDVKPKPVDATDPAALIAEALKKKFAYRYRSDSQDEVEKGIPKSESEATSERVLFG
  PHMLKPTGKMKALIENVSDS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145838
• ORF Size: 900 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145838.1, NP_001139310.1
• RefSeq ORF: 903 bp
• Locus ID: 9650
• UniProt ID: Q15390
• Cytogenetics: 8q13.1
• MW: 33 kDa
• Gene Summary: This gene encodes a mitochondrial protein that is characterized by a poly-proline rich region. A chicken homolog of this protein promotes mitochondrial fission and the mouse homolog protects cells from oxidative stress. A related pseudogene of this gene is found on chromosome X. [provided by RefSeq, Mar 2009]