NBPF6 (NM_001143987) Human Tagged ORF Clone

CAT#: RC227826

NBPF6 (Myc-DDK-tagged)-Human neuroblastoma breakpoint family, member 6 (NBPF6), transcript variant 1

Product Images

Circular map for RC227826

• 

  Western blot validation of overexpression lysate (Cat# LY428447) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227826 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NBPF6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227826 representing NM_001143987
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGTATCTGCCGACCCTTTGTCCAGCGAGAGGGCAGAGATGAACATCCTAGAAATCAACCAGGAAT
  TGCGCTCGCAGCTGGCAGAGAGCAATCAGCAGTTCCGAGACCTCAAAGAGAAATTCCTTATAACTCAAGC
  TACTGCCTACTCCCTGGCCAACCAGCTGAAGAAATACAAGTGTGAAGAGTACAAAGACATCATAGACTCT
  GTGCTGAGGGATGAACTGCAGTCCATGGAGAAGCTGGCAGAGAAGCTCAGGCAAGCTGAGGAGCTCAGGC
  AGTATAAAGCCCTGGTTCACTCTCAGGCAAAAGAGCTGACCCAGTTACGGGAGAAGTTACGGGAAGGGAG
  AGATGCCTCCCGCTGGCTGAACAAGCATCTGAAAACCCTCCTCACTCCTGATGACCCTGACAAGTCCCAG
  GGTCAGGACCTCCGAGAGCAGCTGGCTGAGGGGCACAGGCTGGCAGAGCACCTTGTTCACAAGCTGAGCC
  CAGAAAATGATGAAGATGAAGATGAGGATGAAGACGACAAAGACGAGGAGGTTGAGAAAGTACAGGAATC
  ACCTGCCCCCAGAGAGGTGCAGAAGACTGAAGAAAAGGAAGTCCCTCAGGACTCACTGGAGGAATGTGCT
  GTCACTTGTTCAAATAGTCACAACCCTTCTAACTCCAACCAGCCTCACAGGAGCACCAAAATCACATTTA
  AGGAACACGAAGTCGACTCTGCTCTGGTTGTAGAGAGTGAACACCCTCATGATGAAGAGGAGGAAGCTCT
  AAACATTCCCCCAGAAAATCAAAATGACCATGAGGAGGAGGAGGGGAAAGCGCCAGTGCCCCCCAGGAAC
  CGACAATCACTTGAACCAGGTGAACTGACCAACCTCAGGGATTTCCTGATCTCACCTGTGGTCTCCCATG
  TGGCTAATCCAGGACACCATGACAAGTCCAACTCTTACCGGCATCGTGAAGTCTCTTTCTTGGCATTGGA
  TGAACAGAAAGTTTGCTCCGCTCAGGATGTTGCCAGGGATTACTCCAATCCCAAATGGGATGAAACCTCA
  CTTGGCTTCCTCGAAAAGCAAAGTGATCTTGAAGAGGTGAAAGGACAAGAAACAGTTGCTCCCAGGCTCA
  GCAGGGGACCGCTGAGAGTGGACAAGCATGAAATCCCCCAGGAGTCACTGGATGGATGTTGCTTGACTCC
  TTCCATCCTTCCTGACCTGACTCCCTCCTACCACCCTTATTGGAGCACTTTGTACTCTTTTGAAGACAAG
  CAAGTCAGCTTGGCTCTTGTAGACAAAATTAAAAAGGATCAAGAGGAGATAGAAGACCAAAGCCCACCAT
  GCCCCAGGCTCAGCCAGGAGCTGCCAGAGGTGAAGGAGCAGGAAGTCCCAGAGGACTCTGTGAATGAAGT
  TTACTTGACTCCCTCAGTTCACCATGACGTGTCTGACTGCCACCAGCCTTATAGCAGCACCTTGTCCTCA
  TTGGAGGATCAGCTTGCCTGCTCTGCTCTGGATGTAGCCTCCCCCACCGAGGCGGCCTGTCCCCAAGGGA
  CTTGGAGTGGAGACTTGAGCCACCACCGGTCAGAGGTTCAAATTTCACAGGCACAGCTGGAACCAAGCAC
  CCTGGTGCCCAGTTGTCTGCGACTACAGCTGGATCAAGGGTTCCACTGTGGGAACGGCTTGGCCCAGCGG
  GGCCTTTCCTCCACCACCTGCAGCTTCTCAGCCAATGCTGATTCTGGGAACCAATGGCCCTTCCAAGAGC
  TGGTTTTAGAGCCCTCTCTGGGGATGAAGAACCCTCCCCAGCTGGAAGATGATGCACTTGAAGGCTCAGC
  AAGCAACACACAAGGGCGTCAAGTCACTGGCCGGATTCGTGCCTCCCTTGTCCTGATACTGAAGACCATC
  AGAAGAAGACTCCCGTTCAGCAAGTGGAGACTGGCATTCAGATTCGCTGGCCCGCATGCTGAGAGTGCAG
  AGATACCAAATACTGCTGAAAGGATGCAAAGGATGATAGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227826 representing NM_001143987
  Red=Cloning site Green=Tags(s)
  
  MVVSADPLSSERAEMNILEINQELRSQLAESNQQFRDLKEKFLITQATAYSLANQLKKYKCEEYKDIIDS
  VLRDELQSMEKLAEKLRQAEELRQYKALVHSQAKELTQLREKLREGRDASRWLNKHLKTLLTPDDPDKSQ
  GQDLREQLAEGHRLAEHLVHKLSPENDEDEDEDEDDKDEEVEKVQESPAPREVQKTEEKEVPQDSLEECA
  VTCSNSHNPSNSNQPHRSTKITFKEHEVDSALVVESEHPHDEEEEALNIPPENQNDHEEEEGKAPVPPRN
  RQSLEPGELTNLRDFLISPVVSHVANPGHHDKSNSYRHREVSFLALDEQKVCSAQDVARDYSNPKWDETS
  LGFLEKQSDLEEVKGQETVAPRLSRGPLRVDKHEIPQESLDGCCLTPSILPDLTPSYHPYWSTLYSFEDK
  QVSLALVDKIKKDQEEIEDQSPPCPRLSQELPEVKEQEVPEDSVNEVYLTPSVHHDVSDCHQPYSSTLSS
  LEDQLACSALDVASPTEAACPQGTWSGDLSHHRSEVQISQAQLEPSTLVPSCLRLQLDQGFHCGNGLAQR
  GLSSTTCSFSANADSGNQWPFQELVLEPSLGMKNPPQLEDDALEGSASNTQGRQVTGRIRASLVLILKTI
  RRRLPFSKWRLAFRFAGPHAESAEIPNTAERMQRMIG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001143987
• ORF Size: 2001 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001143987.1, NP_001137459.1
• RefSeq ORF: 2004 bp
• Locus ID: 653149
• UniProt ID: Q5VWK0
• Cytogenetics: 1p13.3
• MW: 75.2 kDa
• Gene Summary: This gene is a member of the neuroblastoma breakpoint family (NBPF) which consists of dozens of recently duplicated genes primarily located in segmental duplications on human chromosome 1. This gene family has experienced its greatest expansion within the human lineage and has expanded, to a lesser extent, among primates in general. Members of this gene family are characterized by tandemly repeated copies of DUF1220 protein domains. Gene copy number variations in the human chromosomal region 1q21.1, where most DUF1220 domains are located, have been implicated in a number of developmental and neurogenetic diseases such as microcephaly, macrocephaly, autism, schizophrenia, cognitive disability, congenital heart disease, neuroblastoma, and congenital kidney and urinary tract anomalies. Altered expression of some gene family members is associated with several types of cancer. This gene family contains numerous pseudogenes. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2013]