TLE6 (NM_001143986) Human Tagged ORF Clone

CAT#: RC227706

TLE6 (Myc-DDK-tagged)-Human transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila) (TLE6), transcript variant 1

Product Images

Circular map for RC227706

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TLE6
• Synonyms: GRG6; PREMBL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227706 representing NM_001143986
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCTCTAGGGACCAGCCCAGACCCAAGGGCCCCCCGAAAAGCACTTCGCCTTGTCCTGGGATCTCGA
  ACTCTGAGAGCTCTCCGACGCTGAATTATCAGGGCATTCTAAATCGGCTCAAGCAGTTCCCCAGGTTTTC
  TCCTCATTTTGCTGCGGAGTTGGAGAGCATTTACTACTCGCTGCACAAGATCCAGCAGGATGTGGCAGAA
  CATCACAAGCAGATAGGAAACGTCTTACAGATTGTGGAGAGCTGCAGCCAACTCCAGGGTTTCCAGTCTG
  AGGAGGTCTCACCTGCTGAACCAGCCAGCCCTGGGACGCCCCAGCAGGTGAAGGACAAGACCCTGCAGGA
  GTCGAGCTTTGAGGACATCATGGCCACCAGGTCCTCCGACTGGCTCCGGCGGCCTTTGGGGGAGGACAAT
  CAGCCGGAGACCCAGCTGTTCTGGGACAAGGAGCCTTGGTTTTGGCACGACACTCTGACCGAGCAACTCT
  GGCGGATTTTTGCCGGCGTCCACGATGAGAAGGCAAAGCCCAGAGACAGACAGCAGGCACCAGGCCTGGG
  GCAGGAAAGCAAGGCACCAGGATCCTGTGACCCAGGAACAGACCCATGTCCTGAAGATGCCTCCACCCCC
  AGGCCACCTGAGGCCTCCTCCAGTCCCCCTGAGGGTTCCCAAGACAGGAACACAAGTTGGGGTGTGGTCC
  AGGAGCCTCCTGGAAGAGCCTCTCGGTTTCTACAGTCCATATCCTGGGACCCTGAGGACTTTGAAGATGC
  ATGGAAGAGGCCAGATGCCTTGCCCGGGCAGTCAAAGAGACTCGCCGTCCCGTGCAAACTGGAAAAGATG
  CGGATCTTGGCACACGGGGAGCTCGTGCTCGCCACGGCCATCAGCAGCTTCACGCGGCACGTGTTCACCT
  GTGGCAGAAGAGGCATCAAGGTGTGGAGCCTGACTGGACAGGTGGCTGAGGACAGGTTCCCTGAGAGCCA
  CCTGCCTATACAGACCCCTGGGGCCTTCCTGCGCACCTGCCTGCTGTCCTCAAACAGCAGGAGCCTGCTC
  ACCGGTGGCTACAACCTGGCCAGCGTGAGCGTGTGGGACCTGGCGGCGCCCTCCCTGCATGTGAAGGAGC
  AGTTGCCCTGTGCAGGTCTCAACTGCCAGGCCCTGGATGCCAACCTGGATGCCAACCTGGCCTTCGCCAG
  CTTCACCAGTGGTGTGGTCAGGATCTGGGACCTGCGGGATCAGAGTGTGGTCAGGGACCTCAAGGGTTAT
  CCTGATGGAGTCAAGAGTATCGTGGTCAAGGGCTACAACATCTGGACTGGGGGTCCGGATGCCTGTCTGC
  GGTGCTGGGACCAGAGGACCATCATGAAACCTCTGGAGTACCAATTCAAGTCTCAGATAATGAGCCTGTC
  CCACAGCCCCCAGGAGGACTGGGTGCTGCTGGGCATGGCCAATGGCCAGCAGTGGCTGCAAAGCACCAGC
  GGGAGCCAGCGGCACATGGTGGGGCAAAAAGACAGCGTCATCCTGAGCGTCAAGTTCTCCCCCTTTGGCC
  AGTGGTGGGCAAGCGTTGGAATGGACGACTTCCTTGGCGTCTACAGCATGCCGGCGGGGACAAAAGTGTT
  CGAGGTGCCTGAGATGTCTCCAGTCACGTGCTGTGACGTCTCTTCCAACAACCGCCTCGTTGTCACAGGC
  TCCGGGGAGCACGCCTCCGTGTACCAGATCACCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227706 representing NM_001143986
  Red=Cloning site Green=Tags(s)
  
  MTSRDQPRPKGPPKSTSPCPGISNSESSPTLNYQGILNRLKQFPRFSPHFAAELESIYYSLHKIQQDVAE
  HHKQIGNVLQIVESCSQLQGFQSEEVSPAEPASPGTPQQVKDKTLQESSFEDIMATRSSDWLRRPLGEDN
  QPETQLFWDKEPWFWHDTLTEQLWRIFAGVHDEKAKPRDRQQAPGLGQESKAPGSCDPGTDPCPEDASTP
  RPPEASSSPPEGSQDRNTSWGVVQEPPGRASRFLQSISWDPEDFEDAWKRPDALPGQSKRLAVPCKLEKM
  RILAHGELVLATAISSFTRHVFTCGRRGIKVWSLTGQVAEDRFPESHLPIQTPGAFLRTCLLSSNSRSLL
  TGGYNLASVSVWDLAAPSLHVKEQLPCAGLNCQALDANLDANLAFASFTSGVVRIWDLRDQSVVRDLKGY
  PDGVKSIVVKGYNIWTGGPDACLRCWDQRTIMKPLEYQFKSQIMSLSHSPQEDWVLLGMANGQQWLQSTS
  GSQRHMVGQKDSVILSVKFSPFGQWWASVGMDDFLGVYSMPAGTKVFEVPEMSPVTCCDVSSNNRLVVTG
  SGEHASVYQITY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001143986
• ORF Size: 1716 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001143986.2
• RefSeq Size: 2033 bp
• RefSeq ORF: 1719 bp
• Locus ID: 79816
• UniProt ID: Q9H808
• Cytogenetics: 19p13.3
• Protein Families: Transcription Factors
• MW: 63.5 kDa
• Gene Summary: This gene encodes a member of the Groucho/ transducin-like Enhancer of split family of transcriptional co-repressors. The encoded protein is a component of the mammalian subcortical maternal complex, which is required for preimplantation development. In mouse, knock out of this gene results in cleavage-stage embryonic arrest resulting from defective cytoplasmic F-actin meshwork formation and asymmetric cell division. In human, an allelic variant in this gene is associated with preimplantation embryonic lethality. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]