RPUSD3 (NM_001142547) Human Tagged ORF Clone

CAT#: RC227215

RPUSD3 (Myc-DDK-tagged)-Human RNA pseudouridylate synthase domain containing 3 (RPUSD3), transcript variant 2

Product Images

Circular map for RC227215

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  Western blot validation of overexpression lysate (Cat# LY428165) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227215 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RPUSD3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227215 representing NM_001142547
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGCTGTCCTGGCTCGGGAGATGGACGGCCGCCGTGTTTTGGGCCGGTTCTGGAGTGGCTGGCGGC
  GGGGCCTGGGTGTCCGCCCAGTGCCCGAGGACGCAGGCTTTGGCACCGAAGCCCGGCATCAGAGGCAACC
  CCGCGGCTCCTGCCAACGGTCGGGGCCCCTCGGGGACCAGCCCTTCGCGGGGCTGCTGCCAAAAAACCTC
  AGTCGGGAGGAGCTGGTTGATGCGCTGCGGGCAGCCGTGGTGGACCGGAAAGGAAAACCAGGAGAGCTGA
  CGTTGTTCTCAGTGCTGCCAGAGCTGAGCCAGTCCCTAGGGCTCAGGGAGCAGGAGCTTCAGGTTGTCCG
  AGCATCTGGGAAAGAAAGCTCTGGGCTTGTACTCCTCTCCAGCTGTCCCCAGACAGCTAGTCGCCTCCAG
  AAGTACTTCACCCATGCACGGAGAGCCCAAAGGCCCACAGCCACCTACTGTGCTGTCACTGATGGGATCC
  CAGCTGCTTCTGAGGGGAAGATCCAGGCTGCCCTGAAACTGGAACACATTGATGGGGTCAATCTCACAGT
  TCCAGTGAAGGCCCCATCCCGAAAGGACATCCTGGAAGGTGTCAAGAAGACTCTCAGTCACTTTCGTGTG
  GTAGCCACAGGCTCTGGCTGTGCCCTGGTCCAGCTGCAGCCACTGACAGTGTTCTCCAGTCAACTACAGG
  TGCACATGGTACTACAGCTCTGCCCTGTGCTTGGGGACCACATGTACTCTGCCCGTGTGGGCACTGTCCT
  GGGCCAGCGATTTCTGCTGCCAGCTGAGAACAACAAGCCCCAAAGACAGGTCCTGGATGAAGCCCTCCTC
  AGACGCCTCCACCTGACCCCCTCCCAGGCTGCCCAGCTGCCCTTGCACCTCCACCTACATCGGCTCCTTC
  TCCCAGGCACCAGGGCCAGGGACACCCCTGTTGAGCTCCTGGCACCACTGCCCCCTTATTTCTCCAGGAC
  CCTACAGTGCCTGGGGCTCCGCTTACAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227215 representing NM_001142547
  Red=Cloning site Green=Tags(s)
  
  MRAVLAREMDGRRVLGRFWSGWRRGLGVRPVPEDAGFGTEARHQRQPRGSCQRSGPLGDQPFAGLLPKNL
  SREELVDALRAAVVDRKGKPGELTLFSVLPELSQSLGLREQELQVVRASGKESSGLVLLSSCPQTASRLQ
  KYFTHARRAQRPTATYCAVTDGIPAASEGKIQAALKLEHIDGVNLTVPVKAPSRKDILEGVKKTLSHFRV
  VATGSGCALVQLQPLTVFSSQLQVHMVLQLCPVLGDHMYSARVGTVLGQRFLLPAENNKPQRQVLDEALL
  RRLHLTPSQAAQLPLHLHLHRLLLPGTRARDTPVELLAPLPPYFSRTLQCLGLRLQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142547
• ORF Size: 1008 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142547.2
• RefSeq ORF: 987 bp
• Locus ID: 285367
• UniProt ID: Q6P087
• Cytogenetics: 3p25.3
• MW: 36.8 kDa
• Gene Summary: This gene encodes a protein that functions in the assembly of the mitochondrial ribosome by adding a pseudouridine group to 16S rRNA. Loss of this gene results in causes defects in mitochondrial protein production. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2017]