GPR113 (ADGRF3) (NM_001145168) Human Tagged ORF Clone

CAT#: RC226960

GPR113 (Myc-DDK-tagged)-Human G protein-coupled receptor 113 (GPR113), transcript variant 1

Product Images

Circular map for RC226960

• 

  Western blot validation of overexpression lysate (Cat# LY428740) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226960 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPR113
• Synonyms: GPR113; PGR23
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226960 representing NM_001145168
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCTGTTCGGCTGCCCCACTGCTGCTCCTGGCCACAACTCTTCCCCTGCTGGGGTCACCAGTTGCCC
  AAGCATCCCAACCTGTAAGTGAGACTGGGGTGAGACCCAGGGAAGGTCTGCAGAGGCGACAATGGGGACC
  CCTGATTGGGAGAGACAAAGCATGGAATGAAAGGATAGACAGACCCTTCCCTGCCTGCCCCATCCCCCTA
  TCTTCTAGCTTTGGCCGATGGCCCAAGGGCCAGACAATGTGGGCCCAGACCTCCACCCTCACCCTGACAG
  AGGAGGAGTTGGGACAGAGTCAGGCTGGAGGGGAATCTGGATCTGGGCAGCTCCTGGACCAAGAGAATGG
  AGCAGGGGAATCAGCGCTGGTCTCCGTCTATGTACATCTGGACTTTCCAGATAAGACCTGGCCCCCTGAA
  CTCTCCAGGACACTGACTCTCCCTGCTGCCTCAGCTTCCTCTTCCCCAAGGCCTCTTCTCACTGGCCTCA
  GACTCACAACAGAGTGTAATGTCAACCACAAGGGGAATTTCTATTGTGCTTGCCTCTCTGGCTACCAGTG
  GAACACCAGCATCTGCCTCCATTACCCTCCTTGTCAAAGCCTCCACAACCACCAGCCTTGTGGCTGCCTT
  GTCTTCAGCCATCCCGAACCCGGGTACTGCCAGTTGCTGCCACCTGGGTCCCCTGTCACCTGCCTCCCTG
  CAGTCCCCGGGATCCTCAACCTGAACTCCCAGCTGCAGATGCCTGGTGACACGCTGAGCCTGACTCTCCA
  TCTGAGCCAGGAGGCCACCAACCTGAGCTGGTTCCTGAGGCACCCAGGGAGCCCCAGTCCCATCCTCCTG
  CAGCCAGGGACACAGGTGTCTGTGACTTCCAGCCACGGCCAGGCTGCCCTCAGCGTCTCCAACATGTCCC
  ATCACTGGGCAGGTGAGTACATGAGCTGCTTCGAGGCCCAGGGCTTCAAGTGGAACCTGTATGAGGTGGT
  GAGGGTGCCCTTGAAGGCGACAGATGTGGCTCGACTTCCATACCAGCTGTCCATCTCCTGTGCCACCTCC
  CCTGGCTTCCAGCTGAGCTGCTGCATCCCCAGCACAAACCTGGCCTACACCGCGGCCTGGAGCCCTGGAG
  AGGGCAGCAAAGCTTCCTCCTTCAACGAGTCAGGCTCTCAGTGCTTTGTGCTGGCTGTTCAGCGCTGCCC
  GATGGCTGACACCACGTACGCTTGTGACCTGCAGAGCCTGGGCCTGGCTCCACTCAGGGTCCCCATCTCC
  ATCACCATCATCCAGGATGGAGACATCACCTGCCCTGAGGACGCCTCGGTGCTCACCTGGAATGTCACCA
  AGGCTGGCCACGTGGCACAGGCCCCATGTCCTGAGAGCAAGAGGGGCATAGTGAGGAGGCTCTGTGGGGC
  TGACGGAGTCTGGGGGCCGGTCCACAGCAGCTGCACAGATGCGAGGCTCCTGGCCTTGTTCACTAGAACC
  AAGCTGCTGCAGGCAGGCCAGGGCAGTCCTGCTGAGGAGGTGCCACAGATCCTGGCACAGCTGCCAGGGC
  AGGCGGCAGAGGCAAGTTCACCCTCCGACTTACTGACCCTGCTGAGCACCATGAAATACGTGGCCAAGGT
  GGTGGCAGAGGCCAGAATACAGCTTGACCGCAGAGCCCTGAAGAATCTCCTGATTGCCACAGACAAGGTC
  CTAGATATGGACACCAGGTCTCTGTGGACCCTGGCCCAAGCCCGGAAGCCCTGGGCAGGCTCGACTCTCC
  TGCTGGCTGTGGAGACCCTGGCATGCAGCCTGTGCCCACAGGACCACCCCTTCGCCTTCAGCTTACCCAA
  TGTGCTGCTGCAGAGCCAGCTGTTTGGACCCACGTTTCCTGCTGACTACAGCATCTCCTTCCCTACTCGG
  CCCCCACTGCAGGCTCAGATTCCCAGGCACTCACTGGCCCCATTGGTCCGTAATGGAACTGAAATAAGTA
  TTACTAGCCTGGTGCTGCGAAAACTGGACCACCTTCTGCCCTCAAACTATGGACAAGGGCTGGGGGATTC
  CCTCTATGCCACTCCTGGCCTGGTCCTTGTCATTTCCATCATGGCAGGTGACCGGGCCTTCAGCCAGGGA
  GAGGTCATCATGGACTTTGGGAACACAGATGGTTCCCCTCACTGTGTCTTCTGGGATCACAGTCTCTTCC
  AGGGCAGGGGGGGTTGGTCCAAAGAAGGGTGCCAGGCACAGGTGGCCAGTGCCAGCCCCACTGCTCAGTG
  CCTCTGCCAGCACCTCACTGCCTTCTCCGTCCTCATGTCCCCACACACTGTTCCGGAAGAACCCGCTCTG
  GCGCTGCTGACTCAAGTGGGCTTGGGAGCTTCCATACTGGCGCTGCTTGTGTGCCTGGGTGTGTACTGGC
  TGGTGTGGAGAGTCGTGGTGCGGAACAAGATCTCCTATTTCCGCCACGCCGCCCTGCTCAACATGGTGTT
  CTGCTTGCTGGCCGCAGACACTTGCTTCCTGGGCGCCCCATTCCTCTCTCCAGGGCCCCGAAGCCCGCTC
  TGCCTTGCTGCCGCCTTCCTCTGTCATTTCCTCTACCTGGCCACCTTTTTCTGGATGCTGGCGCAGGCCC
  TGGTGTTGGCCCACCAGCTGCTCTTTGTCTTTCACCAGCTGGCAAAGCACCGAGTTCTCCCCCTCATGGT
  GCTCCTGGGCTACCTGTGCCCACTGGGGTTGGCAGGTGTCACCCTGGGGCTCTACCTACCTCAAGGGCAA
  TACCTGAGGGAGGGGGAATGCTGGTTGGATGGGAAGGGAGGGGCGTTATACACCTTCGTGGGGCCAGTGC
  TGGCCATCATAGGCGTGAATGGGCTGGTACTAGCCATGGCCATGCTGAAGTTGCTGAGACCTTCGCTGTC
  AGAGGGACCCCCAGCAGAGAAGCGCCAAGCTCTGCTGGGGGTGATCAAAGCCCTGCTCATTCTTACACCC
  ATCTTTGGCCTCACCTGGGGGCTGGGCCTGGCCACTCTGTTAGAGGAAGTCTCCACGGTCCCTCATTACA
  TCTTCACCATTCTCAACACCCTCCAGGGCGTCTTCATCCTATTGTTTGGTTGCCTCATGGACAGGAAGAT
  ACAAGAAGCTTTGCGCAAACGCTTCTGCCGCGCCCAAGCCCCCAGCTCCACCATCTCCCTGGTGAGTTGC
  TGCCTTCAGATCCTCAGCTGTGCATCCAAGAGCATGTCAGAAGGCATTCCATGGCCCTCCTCAGAGGACA
  TGGGCACAGCCAGAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226960 representing NM_001145168
  Red=Cloning site Green=Tags(s)
  
  MVCSAAPLLLLATTLPLLGSPVAQASQPVSETGVRPREGLQRRQWGPLIGRDKAWNERIDRPFPACPIPL
  SSSFGRWPKGQTMWAQTSTLTLTEEELGQSQAGGESGSGQLLDQENGAGESALVSVYVHLDFPDKTWPPE
  LSRTLTLPAASASSSPRPLLTGLRLTTECNVNHKGNFYCACLSGYQWNTSICLHYPPCQSLHNHQPCGCL
  VFSHPEPGYCQLLPPGSPVTCLPAVPGILNLNSQLQMPGDTLSLTLHLSQEATNLSWFLRHPGSPSPILL
  QPGTQVSVTSSHGQAALSVSNMSHHWAGEYMSCFEAQGFKWNLYEVVRVPLKATDVARLPYQLSISCATS
  PGFQLSCCIPSTNLAYTAAWSPGEGSKASSFNESGSQCFVLAVQRCPMADTTYACDLQSLGLAPLRVPIS
  ITIIQDGDITCPEDASVLTWNVTKAGHVAQAPCPESKRGIVRRLCGADGVWGPVHSSCTDARLLALFTRT
  KLLQAGQGSPAEEVPQILAQLPGQAAEASSPSDLLTLLSTMKYVAKVVAEARIQLDRRALKNLLIATDKV
  LDMDTRSLWTLAQARKPWAGSTLLLAVETLACSLCPQDHPFAFSLPNVLLQSQLFGPTFPADYSISFPTR
  PPLQAQIPRHSLAPLVRNGTEISITSLVLRKLDHLLPSNYGQGLGDSLYATPGLVLVISIMAGDRAFSQG
  EVIMDFGNTDGSPHCVFWDHSLFQGRGGWSKEGCQAQVASASPTAQCLCQHLTAFSVLMSPHTVPEEPAL
  ALLTQVGLGASILALLVCLGVYWLVWRVVVRNKISYFRHAALLNMVFCLLAADTCFLGAPFLSPGPRSPL
  CLAAAFLCHFLYLATFFWMLAQALVLAHQLLFVFHQLAKHRVLPLMVLLGYLCPLGLAGVTLGLYLPQGQ
  YLREGECWLDGKGGALYTFVGPVLAIIGVNGLVLAMAMLKLLRPSLSEGPPAEKRQALLGVIKALLILTP
  IFGLTWGLGLATLLEEVSTVPHYIFTILNTLQGVFILLFGCLMDRKIQEALRKRFCRAQAPSSTISLVSC
  CLQILSCASKSMSEGIPWPSSEDMGTARS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145168
• ORF Size: 3237 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145168.1, NP_001138640.1
• RefSeq ORF: 3240 bp
• Locus ID: 165082
• UniProt ID: Q8IZF5
• Cytogenetics: 2p23.3
• Protein Families: Druggable Genome, Transmembrane
• MW: 116.2 kDa
• Gene Summary: Orphan receptor.[UniProtKB/Swiss-Prot Function]