E74 like factor 1 (ELF1) (NM_001145353) Human Tagged ORF Clone

CAT#: RC226778

ELF1 (Myc-DDK-tagged)-Human E74-like factor 1 (ets domain transcription factor) (ELF1), transcript variant 2

Product Images

Circular map for RC226778

• 

  Western blot validation of overexpression lysate (Cat# LY428838) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226778 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E74 like factor 1
• Synonyms: EFTUD1; RIA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226778 representing NM_001145353
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTGTTGTCCAACAGAACGACCTAGTATTTGAATTTGCTAGTAACGTCATGGAGGATGAACGAC
  AGCTTGGTGATCCAGCTATTTTTCCTGCCGTAATTGTGGAACATGTTCCTGGTGCTGATATTCTCAATAG
  TTATGCCGGTCTAGCCTGTGTGGAAGAGCCCAATGACATGATTACTGAGAGTTCACTGGATGTTGCTGAA
  GAAGAAATCATAGACGATGATGATGATGACATCACCCTTACAGTTGAAGCTTCTTGTCATGACGGGGATG
  AAACAATTGAAACTATTGAGGCTGCTGAGGCACTCCTCAATATGGATTCCCCTGGCCCTATGCTGGATGA
  AAAACGAATAAATGGGATTCCTGAAGTGATGGAAACACAGCAGGTGCAAGAAAAATATGCAGACTCACCG
  GGAGCCTCATCACCAGAACAGCCTAAGAGGAAAAAAGGAAGAAAAACTAAACCACCACGACCAGATTCCC
  CAGCCACTACGCCAAATATATCTGTGAAGAAGAAAAACAAAGATGGAAAGGGAAACACAATTTATCTTTG
  GGAGTTTTTACTGGCACTGCTCCAGGACAAGGCTACTTGTCCTAAATACATCAAGTGGACCCAGCGAGAG
  AAAGGCATTTTTAAATTGGTGGATTCTAAAGCAGTGTCCAGGTTGTGGGGGAAGCACAAAAACAAACCTG
  ATATGAATTATGAGACCATGGGAAGAGCACTCAGGTACTATTACCAAAGGGGTATTCTGGCAAAAGTGGA
  AGGTCAGCGCTTGGTGTATCAGTTTAAAGAAATGCCAAAAGATCTTATATATATAAATGATGAGGATCCA
  AGTTCCAGCATAGAGTCTTCAGATCCATCGCTATCTTCATCAGCCACTTCAAATAGGAATCAAACCAGCC
  GGTCGAGAGTATCTTCAAGTCCAGGGGTAAAAGGAGGAGCCACTACAGTTCTAAAACCAGGGAATTCTAA
  AGCTGCAAAACCCAAAGATCCTGTGGAAGTTGCACAACCATCAGAAGTTTTGAGGACAGTGCAGCCCACG
  CAGTCTCCATATCCTACCCAGCTCTTCCGGACTGTTCATGTAGTACAGCCAGTACAGGCTGTCCCAGAGG
  GAGAAGCAGCTAGAACCAGTACCATGCAGGATGAAACATTAAATTCTTCCGTTCAGAGTATTAGGACTAT
  ACAGGCTCCAACCCAAGTTCCAGTGGTTGTGTCTCCTAGGAATCAGCAGTTGCATACAGTAACACTCCAA
  ACAGTGCCACTCACAACAGTTATAGCCAGCACAGATCCATCAGCAGGTACTGGATCTCAGAAGTTTATTT
  TACAAGCCATTCCATCATCACAGCCCATGACAGTACTGAAAGAAAATGTCATGCTGCAGTCACAAAAGGC
  GGGCTCTCCTCCTTCAATTGTCTTGGGCCCTGCCCAGGTTCAGCAGGTCCTTACTAGCAATGTTCAGACC
  ATTTGCAATGGAACCGTCAGTGTGGCTTCCTCTCCATCCTTCAGTGCTACTGCACCTGTGGTGACCTTTT
  CTCCTCGCAGTTCACAGCTGGTTGCTCACCCACCTGGCACTGTAATCACTTCAGTTATCAAAACTCAAGA
  AACAAAAACTCTTACACAGGAAGTAGAGAAAAAGGAATCTGAAGATCATTTGAAAGAGAACACTGAGAAA
  ACGGAGCAGCAGCCACAGCCTTATGTGATGGTAGTGTCCAGTTCCAATGGATTTACTTCTCAGGTAGCTA
  TGAAACAAAACGAACTGCTGGAACCCAACTCTTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226778 representing NM_001145353
  Red=Cloning site Green=Tags(s)
  
  MAAVVQQNDLVFEFASNVMEDERQLGDPAIFPAVIVEHVPGADILNSYAGLACVEEPNDMITESSLDVAE
  EEIIDDDDDDITLTVEASCHDGDETIETIEAAEALLNMDSPGPMLDEKRINGIPEVMETQQVQEKYADSP
  GASSPEQPKRKKGRKTKPPRPDSPATTPNISVKKKNKDGKGNTIYLWEFLLALLQDKATCPKYIKWTQRE
  KGIFKLVDSKAVSRLWGKHKNKPDMNYETMGRALRYYYQRGILAKVEGQRLVYQFKEMPKDLIYINDEDP
  SSSIESSDPSLSSSATSNRNQTSRSRVSSSPGVKGGATTVLKPGNSKAAKPKDPVEVAQPSEVLRTVQPT
  QSPYPTQLFRTVHVVQPVQAVPEGEAARTSTMQDETLNSSVQSIRTIQAPTQVPVVVSPRNQQLHTVTLQ
  TVPLTTVIASTDPSAGTGSQKFILQAIPSSQPMTVLKENVMLQSQKAGSPPSIVLGPAQVQQVLTSNVQT
  ICNGTVSVASSPSFSATAPVVTFSPRSSQLVAHPPGTVITSVIKTQETKTLTQEVEKKESEDHLKENTEK
  TEQQPQPYVMVVSSSNGFTSQVAMKQNELLEPNSF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145353
• ORF Size: 1785 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145353.1, NP_001138825.1
• RefSeq ORF: 1788 bp
• Locus ID: 1997
• UniProt ID: P32519
• Cytogenetics: 13q14.11
• Protein Families: Transcription Factors
• MW: 64.7 kDa
• Gene Summary: This gene encodes an E26 transformation-specific related transcription factor. The encoded protein is primarily expressed in lymphoid cells and acts as both an enhancer and a repressor to regulate transcription of various genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2009]