KCP (NM_001135914) Human Tagged ORF Clone

CAT#: RC226749

KCP (Myc-DDK-tagged)-Human kielin/chordin-like protein (KCP), transcript variant 1

Product Images

Circular map for RC226749

• 

  Western blot validation of overexpression lysate (Cat# LY427728) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226749 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCP
• Synonyms: CRIM2; KCP1; NET67
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226749 representing NM_001135914
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGGGTCGGGGCCGCTGCGCTGTCCCTTCTCCTGCACCTCGGGGCCCTGGCGCTGGCCGCGGGCG
  CGGAAGGTGGGGCTGTCCCCAGGGAGCCCCCTGGGCAGCAGACAACTGCCCATTCCTCAGTCCTTGCTGG
  GAACTCCCAGGAGCAGTGGCACCCCCTGCGAGAGTGGCTGGGGCGACTGGAGGCTGCAGTGATGGAGCTC
  AGAGAACAGAATAAGGACCTGCAGACGAGGGTGAGGCAGCTGGAGTCCTGTGAGTGCCACCCTGCATCTC
  CCCAGTGCTGGGGGCTGGGGCGTGCCTGGCCCGAGGGGGCACGCTGGGAGCCTGACGCCTGCACAGCCTG
  CGTCTGCCAGGATGGGGCCGCTCACTGTGGCCCCCAAGCACACCTGCCCCATTGCAGGGGCTGCAGCCAA
  AATGGCCAGACCTACGGCAACGGGGAGACCTTCTCCCCAGATGCCTGCACCACCTGCCGCTGTCTGACAG
  GAGCCGTGCAGTGCCAGGGGCCCTCGTGTTCAGAGCTCAACTGCTTGGAGAGCTGCACCCCACCTGGGGA
  GTGCTGCCCCATCTGCTGCACAGAAGGTGGCTCTCACTGGGAACATGGCCAAGAGTGGACAACACCTGGG
  GACCCCTGCCGAATCTGCCGGTGCCTGGAGGGTCACATCCAGTGCCGCCAGCGAGAATGTGCCAGCCTGT
  GTCCATACCCAGCCCGGCCCCTCCCAGGCACCTGCTGCCCTGTGTGTGATGGCTGTTTCCTAAACGGGCG
  GGAGCACCGCAGCGGGGAGCCTGTGGGCTCAGGGGACCCCTGCTCGCACTGCCGCTGTGCTAATGGGAGT
  GTCCAGTGTGAGCCTCTGCCCTGCCCGCCAGTGCCCTGCAGACACCCAGGCAAGATCCCTGGGCAGTGCT
  GCCCTGTCTGCGATGGCTGTGAGTACCAGGGACACCAGTATCAGAGCCAGGAGACCTTCAGACTCCAAGA
  GCGGGGCCTCTGTGTCCGCTGCTCCTGCCAGGCTGGCGAGGTCTCCTGTGAGGAGCAGGAGTGCCCAGTC
  ACCCCCTGTGCCCTGCCTGCCTCTGGCCGCCAGCTCTGCCCAGCCTGTGAGCTGGATGGAGAGGAGTTTG
  CTGAGGGAGTCCAGTGGGAGCCTGATGGTCGGCCCTGCACCGCCTGCGTCTGTCAAGATGGGGTACCCAA
  GTGCGGGGCTGTGCTCTGCCCCCCAGCCCCCTGCCAGCACCCCACCCAGCCCCCTGGTGCCTGCTGCCCC
  AGCTGTGACAGCTGCACCTACCACAGCCAAGTGTATGCCAATGGGCAGAACTTCACGGATGCAGACAGCC
  CTTGCCATGCCTGCCACTGTCAGGATGGAACTGTGACATGCTCCTTGGTTGACTGCCCTCCCACGACCTG
  TGCCAGGCCCCAGAGTGGACCAGGCCAGTGTTGCCCCAGGTGCCCAGACTGCATCCTGGAGGAAGAGGTG
  TTTGTGGACGGCGAGAGCTTCTCCCACCCCCGAGACCCCTGCCAGGAGTGCCGATGCCAGGAAGGCCATG
  CCCACTGCCAGCCTCGCCCCTGCCCCAGGGCCCCCTGTGCCCACCCGCTGCCTGGGACCTGCTGCCCGAA
  CGACTGCAGCGGCTGTGCCTTTGGCGGGAAAGAGTACCCCAGCGGAGCGGACTTCCCCCACCCCTCTGAC
  CCCTGCCGTCTGTGTCGCTGTCTGAGCGGCAACGTGCAGTGCCTGGCCCGCCGCTGCGTGCCGCTGCCCT
  GTCCAGAGCCTGTCCTGCTGCCGGGAGAGTGCTGCCCGCAGTGCCCAGCCCCCGCCGGCTGCCCACGGCC
  CGGCGCGGCCCACGCCCGCCACCAGGAGTACTTCTCCCCGCCCGGCGATCCCTGCCGCCGCTGCCTCTGC
  CTCGACGGCTCCGTGTCCTGCCAGCGGCTGCCCTGCCCGCCCGCGCCCTGCGCGCACCCGCGCCAGGGGC
  CTTGCTGCCCCTCCTGCGACGGCTGCCTGTACCAGGGGAAGGAGTTTGCCAGCGGGGAGCGCTTCCCATC
  GCCCACTGCTGCCTGCCACCTCTGCCTTTGCTGGGAGGGCAGCGTGAGCTGCGAGCCCAAGGCATGTGCC
  CCTGCACTGTGCCCCTTCCCTGCCAGGGGCGACTGCTGCCCTGACTGTGATGGCTGTGAGTACCTGGGGG
  AGTCCTACCTGAGTAACCAGGAGTTCCCAGACCCCCGAGAACCCTGCAACCTGTGTACCTGTCTTGGAGG
  CTTCGTGACCTGCGGCCGCCGGCCCTGTGAGCCTCCGGGCTGCAGCCACCCACTCATCCCCTCTGGGCAC
  TGCTGCCCGACCTGCCAGGGATGCCGCTACCATGGCGTCACTACTGCCTCCGGAGAGACCCTTCCTGACC
  CACTTGACCCTACCTGCTCCCTCTGCACCTGCCAGGAAGGTTCCATGCGCTGCCAGAAGAAGCCATGTCC
  CCCAGCTCTCTGCCCCCACCCCTCTCCAGGCCCCTGCTTCTGCCCTGTTTGCCACAGCTGTCTCTCTCAG
  GGCCGGGAGCACCAGGATGGGGAGGAGTTTGAGGGACCAGCAGGCAGCTGTGAGTGGTGTCGCTGTCAGG
  CTGGCCAGGTCAGCTGTGTGCGGCTGCAGTGCCCACCCCTTCCCTGCAAGCTCCAGGTCACCGAGCGGGG
  GAGCTGCTGCCCTCGCTGCAGAGGCTGCCTGGCTCATGGGGAAGAGCACCCCGAAGGCAGTAGATGGGTG
  CCCCCCGACAGTGCCTGCTCCTCCTGTGTGTGTCACGAGGGCGTCGTCACCTGTGCACGCATCCAGTGCA
  TCAGCTCTTGCGCCCAGCCCCGCCAAGGGCCCCATGACTGCTGTCCTCAATGCTCTGACTGTGAGCATGA
  GGGCCGGAAGTACGAGCCTGGGGAGAGCTTCCAGCCTGGGGCAGACCCCTGTGAAGTGTGCATCTGCGAG
  CCACAGCCTGAGGGGCCTCCCAGCCTTCGCTGTCACCGGCGGCAGTGTCCCAGCCTGGTGGGCTGCCCCC
  CCAGCCAGCTCCTGCCCCCTGGGCCCCAGCACTGCTGTCCCACCTGTGCCGAGGCCTTGAGTAACTGTTC
  AGAGGGCCTGCTGGGATCTGAGCTAGCCCCACCAGACCCCTGCTACACGTGCCAGTGCCAGGACCTGACA
  TGGCTCTGCATCCACCAGGCTTGTCCTGAGCTCAGCTGTCCCCTCTCAGAGCGCCACACTCCCCCTGGGA
  GCTGCTGCCCCGTATGCCGGGCTCCCACCCAGTCCTGCGTGCACCAGGGCCGTGAGGTGGCCTCTGGAGA
  GCGCTGGACTGTGGACACCTGCACCAGCTGCTCCTGCATGGCGGGCACCGTGCGTTGCCAGAGCCAGCGC
  TGCTCACCGCTCTCGTGTGGCCCCGACAAGGCCCCTGCCCTGAGTCCTGGCAGCTGCTGCCCCCGCTGCC
  TGCCTCGGCCCGCTTCCTGCATGGCCTTCGGAGACCCCCATTACCGCACCTTCGACGGCCGCCTGCTGCA
  CTTCCAGGGCAGTTGCAGCTATGTGCTGGCCAAGGACTGCCACAGCGGGGACTTCAGTGTGCACGTGACC
  AATGATGACCGGGGCCGGAGCGGTGTGGCCTGGACCCAGGAGGTGGCGGTGCTGCTGGGAGACATGGCCG
  TGCGGCTGCTGCAGGACGGGGCAGTCACGGTGGATGGGCACCCGGTGGCCTTGCCCTTCCTGCAGGAGCC
  GCTGCTGTATGTGGAGCTGCGAGGACACACTGTGATCCTGCACGCCCAGCCCGGGCTCCAGGTGCTGTGG
  GATGGGCAGTCCCAGGTGGAGGTGAGCGTACCTGGCTCCTACCAGGGCCGGACTTGTGGGCTCTGTGGGA
  ACTTCAATGGCTTTGCCCAGGACGATCTGCAGGGCCCTGAGGGGCTGCTCCTGCCCTCGGAGGCTGCGTT
  TGGGAATAGCTGGCAGGTCTCAGAGGGGCTGTGGCCTGGCCGGCCCTGTTCTGCAGGCCGAGAGGTGGAT
  CCGTGCCGGGCAGCAGGTTACCGTGCCAGGCGTGAGGCCAATGCCCGGTGTGGGGTGCTGAAGTCCTCCC
  CATTCAGTCGCTGCCATGCTGTGGTGCCACCGGAGCCCTTCTTTGCCGCCTGTGTGTATGACCTGTGTGC
  CTGTGGCCCTGGCTCCTCCGCTGATGCCTGCCTCTGTGATGCCCTGGAAGCCTACGCCAGTCACTGTCGC
  CAGGCAGGAGTGACACCTACCTGGCGAGGCCCCACGCTGTGTGTGGTAGGCTGCCCCCTGGAGCGTGGCT
  TCGTGTTTGATGAGTGCGGCCCACCCTGTCCCCGCACCTGCTTCAATCAGCATATCCCCCTGGGGGAGCT
  GGCAGCCCACTGCGTGAGGCCCTGCGTGCCCGGCTGCCAGTGCCCTGCAGGCCTGGTGGAGCATGAGGCC
  CACTGCATCCCACCCGAGGCCTGCCCCCAAGTCCTGCTCACTGGAGACCAGCCACTTGGTGCTCGGCCCA
  GCCCCAGCCGGGAGCCCCAGGAGACACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226749 representing NM_001135914
  Red=Cloning site Green=Tags(s)
  
  MAGVGAAALSLLLHLGALALAAGAEGGAVPREPPGQQTTAHSSVLAGNSQEQWHPLREWLGRLEAAVMEL
  REQNKDLQTRVRQLESCECHPASPQCWGLGRAWPEGARWEPDACTACVCQDGAAHCGPQAHLPHCRGCSQ
  NGQTYGNGETFSPDACTTCRCLTGAVQCQGPSCSELNCLESCTPPGECCPICCTEGGSHWEHGQEWTTPG
  DPCRICRCLEGHIQCRQRECASLCPYPARPLPGTCCPVCDGCFLNGREHRSGEPVGSGDPCSHCRCANGS
  VQCEPLPCPPVPCRHPGKIPGQCCPVCDGCEYQGHQYQSQETFRLQERGLCVRCSCQAGEVSCEEQECPV
  TPCALPASGRQLCPACELDGEEFAEGVQWEPDGRPCTACVCQDGVPKCGAVLCPPAPCQHPTQPPGACCP
  SCDSCTYHSQVYANGQNFTDADSPCHACHCQDGTVTCSLVDCPPTTCARPQSGPGQCCPRCPDCILEEEV
  FVDGESFSHPRDPCQECRCQEGHAHCQPRPCPRAPCAHPLPGTCCPNDCSGCAFGGKEYPSGADFPHPSD
  PCRLCRCLSGNVQCLARRCVPLPCPEPVLLPGECCPQCPAPAGCPRPGAAHARHQEYFSPPGDPCRRCLC
  LDGSVSCQRLPCPPAPCAHPRQGPCCPSCDGCLYQGKEFASGERFPSPTAACHLCLCWEGSVSCEPKACA
  PALCPFPARGDCCPDCDGCEYLGESYLSNQEFPDPREPCNLCTCLGGFVTCGRRPCEPPGCSHPLIPSGH
  CCPTCQGCRYHGVTTASGETLPDPLDPTCSLCTCQEGSMRCQKKPCPPALCPHPSPGPCFCPVCHSCLSQ
  GREHQDGEEFEGPAGSCEWCRCQAGQVSCVRLQCPPLPCKLQVTERGSCCPRCRGCLAHGEEHPEGSRWV
  PPDSACSSCVCHEGVVTCARIQCISSCAQPRQGPHDCCPQCSDCEHEGRKYEPGESFQPGADPCEVCICE
  PQPEGPPSLRCHRRQCPSLVGCPPSQLLPPGPQHCCPTCAEALSNCSEGLLGSELAPPDPCYTCQCQDLT
  WLCIHQACPELSCPLSERHTPPGSCCPVCRAPTQSCVHQGREVASGERWTVDTCTSCSCMAGTVRCQSQR
  CSPLSCGPDKAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCHSGDFSVHVT
  NDDRGRSGVAWTQEVAVLLGDMAVRLLQDGAVTVDGHPVALPFLQEPLLYVELRGHTVILHAQPGLQVLW
  DGQSQVEVSVPGSYQGRTCGLCGNFNGFAQDDLQGPEGLLLPSEAAFGNSWQVSEGLWPGRPCSAGREVD
  PCRAAGYRARREANARCGVLKSSPFSRCHAVVPPEPFFAACVYDLCACGPGSSADACLCDALEAYASHCR
  QAGVTPTWRGPTLCVVGCPLERGFVFDECGPPCPRTCFNQHIPLGELAAHCVRPCVPGCQCPAGLVEHEA
  HCIPPEACPQVLLTGDQPLGARPSPSREPQETP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001135914
• ORF Size: 4509 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001135914.1, NP_001129386.1
• RefSeq ORF: 4512 bp
• Locus ID: 375616
• UniProt ID: Q6ZWJ8
• Cytogenetics: 7q32.1
• MW: 159.8 kDa
• Gene Summary: Enhances bone morphogenetic protein (BMP) signaling in a paracrine manner. In contrast, it inhibits both the activin-A and TGFB1-mediated signaling pathways (By similarity).[UniProtKB/Swiss-Prot Function]