RGPD4 (NM_182588) Human Tagged ORF Clone

CAT#: RC226564

RGPD4 (Myc-DDK-tagged)-Human RANBP2-like and GRIP domain containing 4 (RGPD4)

Product Images

Circular map for RC226564

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RGPD4
• Synonyms: RGP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226564 representing NM_182588
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTGCAGCAAGGCCTACGGGGAGCGGTACGTCGCCTCCGTGCAGGGCTCCGCCCCGTCGCCTCGAA
  AGAAGTCAACGAGAGGATTCTATTTTGCAAAGCTGTATTATGAAGCTAAAGAATATGATCTTGCTAAAAA
  ATACATATGTACTTACATTAATGTGCGAGAGATGGATCCCAGAGCTCACAGATTTCTGGGTCTTCTTTAT
  GAATTGGAAGAAAACACAGAGAAAGCCGTTGAATGTTACAGGCGTTCAGTGGAATTAAACCCAACACAAA
  AAGATCTGGTGTTGAAGATTGCAGAATTGCTTTGTAAAAATGATGTTACTGATGGAAGAGCAAAATACTG
  GGTGGAAAGAGCAGCAAAACTTTTCCCAGGAAGTCCTGCAATTTATAAACTAAAGGAACAGCTTCTAGAT
  TGTGAAGGTGAAGATGGATGGAATAAACTTTTTGACTTGATTCAGTCAGAACTTTATGTAAGACCTGATG
  ACGTCCATGTGAACATCCGGCTAGTGGAGTTGTATCGCTCAACTAAAAGATTGAAGGATGCTGTGGCCCG
  CTGCCATGAGGCAGAGAGGAACATAGCTTTGCGTTCAAGTTTAGAGTGGAATTCGTGTGTTGTACAGACC
  CTTAAGGAATATCTGGAGTCTTTACAGTGTTTGGAGTCTGATAAAAGTGACTGGCGAGCAACCAATACAG
  ACTTACTGCTGGCCTATGCTAATCTTATGCTTCTTACGCTTTCCACTAGAGATGTGCAGGAAAGTAGAGA
  ATTACTGGAAAGTTTTGATAGTGCTCTTCAGTCTGCGAAATCTTCTTTGGGTGGAAATGATGAACTGTCA
  GCTACTTTCTTAGAAATGAAAGGACATTTCTACATGCATGCTGGTTCTCTGCTCTTGAAGATGGGTCAGC
  ATGGTAATAATGTTCAATGGCGAGCTCTTTCTGAGCTGGCTGCATTGTGCTATCTCATAGCATTTCAGGT
  TCCAAGACCAAAGATTAAATTAATAAAAGGTGAAGCTGGACAAAATCTGCTGGAAATGATGGCCTGTGAC
  CGACTGAGCCAATCAGGGCACATGTTGCTAAACTTAAGTCGTGGCAAGCAAGATTTTTTAAAAGTGGTTG
  TTGAAACTTTTGCCAACAAAAGCGGGCAGTCTGCATTATATGATGCTCTGTTTTCTAGTCAGTCACCTAA
  GGATACATCTTTTCTTGGTAGCGATGATATTGGAAACATTGATGTACAAGAACCAGAGCTTGAAGATTTG
  GCTAGATACGATGTTGGTGCTATTCGAGCACATAATGGTAGTCTTCAGCACCTTACTTGGCTTGGCTTAC
  AGTGGAATTCATTGCCTGCTTTACCTGGAATCCGAAAATGGCTAAAACAGCTTTTCCATCATTTGCCCCA
  GGAAACCTCAAGGCTTGAAACAAATGCACCTGAATCAATATGTATTTTAGATCTTGAAGTATTTCTCCTT
  GGAGTAGTATATACCAGCCACTTACAATTAAAGGAGAAATGTAATTCTCACCACAGCTCCTATCAGCCGT
  TATGCCTGCCCCTTCCTGTATGTAAACAGCTTTGTACAGAAAGACAAAAATCTTGGTGGGATGCGGTTTG
  TACTCTGATTCACAGAAAAGCAGTACCTGGAAACTCAGCAAAATTGAGACTTTTAGTTCAGCATGAAATA
  AACACTCTAAGAGCCCAGGAAAAACATGGCCTTCAACCTGCTCTGCTTGTACATTGGGCAAAATGCCTTC
  AGAAAATGGGCAGTGGTCTTAATTCTTTTTATGATCAACGAGAATACATAGGGAGAAGTGTTCATTATTG
  GAAGAAAGTTTTGCCATTGTTGAAGATAATAAAAAAGAAGAACAGTATTCCTGAACCTATTGATCCTCTG
  TTTAAACATTTTCATAGTGTAGACATTCAGGCATCAGAAATTGTTGAATATGAAGAAGACGCACACGTAA
  CTTTTGCTATATTGGATGCAGTAAATGGAAATATAGAAGATGCTATGACTGCTTTTGAATCTATAAAAAG
  TGTTGTTTCTTATTGGAATCTTGCACTGATTTTTCACAGGAAGGCAGAAGACATTGCAAATGATGCCCTT
  TCTCCTGAAGAACAAGAAGAATGCAAAAATTATCTGAGAAAGACCAGGGGCTACCTAATAAAGATTTTAG
  ATGACAGTGATTCAAATCTTTCAGTGGTCAAGAAATTGCCTGTGCCCCTGGAGTCTGTAAAAGAGATGCT
  TAAGTCAGTCATGCAGGAACTCGAAAACTATAGTGAAGGAGATCCTCTCTATAAAAATGGTTCTTTGCGA
  AATGCGGATTCAGAAATAAAACATTCTACACCATCTCCTACCAAATATTCACTATCACCAAGTAAAAGTT
  ACAAGTATTCTCCCAAAACACCACCTCGATGGGCAGAAGATCAGAATTCTTTACTGAAAATGATTCGCCA
  AGAAGTAAAGGCCATTAAGGAAGAAATGCAGGAGTTGAAACTAAATAGCAGTAAGTCAGCATCCCATCAT
  CGTTGGCCCACAGAGAATTATGGACCAGACTCGGTGCCTGATGGATATCAGGGGTCACAGACATTTCATG
  GGGCTCCACTAACAGTTGCAACTACTGGCCCTTCAGTATATTATAGTCAGTCACCAGCATATAATTCCCA
  GTATCTTCTCAGACCAGCAGCTAATGTTACTCCCACAAAGGGTTCTTCTAATACAGAATTTAAGTCAACC
  AAAGAAGGATTTTCCATCCCTGTGTCTGCTGATGGATTTAAATTTGGCATTTCGGAACCAGGAAATCAAG
  AAAAGGAAAGTGAAAAGCCTCTTGAAAATGATACTGGCTTCCAGGCTCAGGATATTAGTGGCCAGAAGAA
  TGGCCGTGGTGTGATTTTTGGCCAAACAAGTAGCACTTTTACATTTGCAGATGTTGCAAAATCAACTTCA
  GGAGAAGGATTTCAGTTTGGCAAAAAAGACCCCAATTTCAAGGGATTTTCAGGTGCTGGAGAAAAATTAT
  TCTCATCACAATGCGGTAAAATGGCCAATAAAGCAAACACTTCCGGTGACTTTGAGAAAGATGATGATGC
  CTATAAGACTGAGGACAGCGATGACATCCATTTTGAACCAGTAGTTCAAATGCCTGAAAAAGTAGAACTT
  GTAATAGGAGAAGAAGGTGAAAAAGTTCTGTATTCACAGGGGGTAAAACTATTTAGATTTGATGCTGAGG
  TAAGGCAGTGGAAAGAAAGGGGCTTGGGGAACTTAAAAATTCTCAAAAACGAGGTCAATGGCAAACCAAG
  AATGCTGATGCGAAGAGAACAAGTACTAAAAGTGTGTGCTAATCATTGGATAACGACTACAATGAACCTG
  AAGCCCCTGTCTGGATCAGATAGAGCATGGATGTGGTCAGCCAGTGATTTCTCTGATGGTGATGCCAAAC
  TAGAGCGGTTAGCAGCAAAATTTAAAACACCAGAGCTGGCTGAAGAATTCAAGCAGAAATTTGAGGAATG
  CCAGCAGCTTCTGTTAGACATACCACTTCAAACTCCCCATAAACTTGTAGATACTGGCAGAGCTGCCAAG
  TTAATACAGAGAGCTGAAGAAATGAAGAGTGGACTGAAAGATTTCAAAACATTTTTGACAAATGATCAAA
  CAAAAGTCACTGAGGAAGAAAATAAGGGTTCAGGTACAGGTGCGGCCGGTGCCTCAGACACAACAATAAA
  ACCCAATCCTGAAAACACTGGGCCCACATTAGAATGGGATAACTGTGATTTAAGGGAAGATGCTTTGGAT
  GATAGTGTCAGTAGTAGCTCAGTACATGCTTCTCCATTGGCAAGTAGCCCTGTGAGAAAAAATCTTTTCC
  ATTTTGGTGAGTCAACAACAGGATCTAACTTCAGTTTTAAATCTGCTTTGAGTCCATCTAAGTCTCCTGC
  CAAGTTGAATCAGAGTGGGACTTCAGTTGGCACTGATGAAGAATCTGATGTTACTCAAGAAGAAGAGAGA
  GATGGACAGTACTTTGAACCTGTTGTTCCTTTACCTGATCTAGTTGAAGTATCCAGTGGTGAGGAAAATG
  AAAAAGTTGTTTTTAGTCACAGGGCAGAACTCTACAGATATGATAAAGATGTTGGTCAATGGAAAGAAAG
  GGGCATTGGTGATATAAAGATTTTACAGAATTATGATAATAAGCAAGTTCGTATAGTGATGAGAAGGGAC
  CAAGTATTAAAACTTTGTGCCAATCACACAATAACTCCAGACATGAGTTTGCAAAATATGAAAGGGACAG
  AAAGAGTATGGGTGTGGACTGCATGTGATTTTGCAGATGGAGAAAGAAAAGTAGAGCATTTAGCTGTTCG
  TTTTAAACTACAGGATGTTGCAGACTCGTTTAAGAAAATTTTTGATGAAGCAAAAACAGCCCAGGAAAAA
  GATTCTTTGATAACACCTCATGTTTCTCGGTCAAGCACTCCCAGAGAGTCACCATGTGGCAAAATTGCTG
  TAGCTGTATTAGAAGAAACCACAAGAGAGAGGACAGATGTTATTCAGGGTGATGATGTAGCAGATGCAGC
  TTCAGAAGTTGAAGTGTCTAGCACATCTGAAACAACAACAAAAGCAGTGGTTTCTCCTCCAAAGTTTGTA
  TTTGGTTCAGAGTCTGTTAAAAGAATTTTTAGTAGTGAAAAATCAAAACCATTTGCATTTGGCAACAGTT
  CTGCCACTGGGTCTTTGTTTGGATTTAGTTTTAATGCATCTTTGAAAAGTAACAACAGTGAAACTAGTTC
  AGTAGCCCAGAGTGGATCTGAAAGCAAAGTGGAACCTAAAAAATGTGAACTGTCAAAGAACTCTGATATC
  GAACAGTCTTCAGATAGCAAAGTCAAAAATCTCTCTGCTTCCTTTCCAATGGAAGAATCTTCAATCAACT
  ACACATTTAAAACACCAGAAAAGGAGCCTCCATTATGGCATGCTGAATTTACCAAAGAAGAATTGGTTCA
  GAAGCTCAGTTCCACCACAAAAAGTGCAGATCACTTAAACGGCCTGCTTCGGGAAGCAGAGGCAACCAGT
  GCAGTCCTTATGGAGCAAATTAAGCTTCTCAAAAGTGAAATAAGAAGATTGGAAAGGAATCAAGAGCAAG
  AGGAGTCTGCAGCTAACGTGGAACACTTGAAGAACGTCTTGCTGCAGTTCATTTTCTTGAAGCCAGGTAG
  TGAAAGAGAGAGACTTCTTCCTGTTATAAATACGATGTTGCAGCTCAGCCCTGAAGAAAAGGGAAAACTT
  GCTGCGGTTGCTCAAGGTGAGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226564 representing NM_182588
  Red=Cloning site Green=Tags(s)
  
  MSCSKAYGERYVASVQGSAPSPRKKSTRGFYFAKLYYEAKEYDLAKKYICTYINVREMDPRAHRFLGLLY
  ELEENTEKAVECYRRSVELNPTQKDLVLKIAELLCKNDVTDGRAKYWVERAAKLFPGSPAIYKLKEQLLD
  CEGEDGWNKLFDLIQSELYVRPDDVHVNIRLVELYRSTKRLKDAVARCHEAERNIALRSSLEWNSCVVQT
  LKEYLESLQCLESDKSDWRATNTDLLLAYANLMLLTLSTRDVQESRELLESFDSALQSAKSSLGGNDELS
  ATFLEMKGHFYMHAGSLLLKMGQHGNNVQWRALSELAALCYLIAFQVPRPKIKLIKGEAGQNLLEMMACD
  RLSQSGHMLLNLSRGKQDFLKVVVETFANKSGQSALYDALFSSQSPKDTSFLGSDDIGNIDVQEPELEDL
  ARYDVGAIRAHNGSLQHLTWLGLQWNSLPALPGIRKWLKQLFHHLPQETSRLETNAPESICILDLEVFLL
  GVVYTSHLQLKEKCNSHHSSYQPLCLPLPVCKQLCTERQKSWWDAVCTLIHRKAVPGNSAKLRLLVQHEI
  NTLRAQEKHGLQPALLVHWAKCLQKMGSGLNSFYDQREYIGRSVHYWKKVLPLLKIIKKKNSIPEPIDPL
  FKHFHSVDIQASEIVEYEEDAHVTFAILDAVNGNIEDAMTAFESIKSVVSYWNLALIFHRKAEDIANDAL
  SPEEQEECKNYLRKTRGYLIKILDDSDSNLSVVKKLPVPLESVKEMLKSVMQELENYSEGDPLYKNGSLR
  NADSEIKHSTPSPTKYSLSPSKSYKYSPKTPPRWAEDQNSLLKMIRQEVKAIKEEMQELKLNSSKSASHH
  RWPTENYGPDSVPDGYQGSQTFHGAPLTVATTGPSVYYSQSPAYNSQYLLRPAANVTPTKGSSNTEFKST
  KEGFSIPVSADGFKFGISEPGNQEKESEKPLENDTGFQAQDISGQKNGRGVIFGQTSSTFTFADVAKSTS
  GEGFQFGKKDPNFKGFSGAGEKLFSSQCGKMANKANTSGDFEKDDDAYKTEDSDDIHFEPVVQMPEKVEL
  VIGEEGEKVLYSQGVKLFRFDAEVRQWKERGLGNLKILKNEVNGKPRMLMRREQVLKVCANHWITTTMNL
  KPLSGSDRAWMWSASDFSDGDAKLERLAAKFKTPELAEEFKQKFEECQQLLLDIPLQTPHKLVDTGRAAK
  LIQRAEEMKSGLKDFKTFLTNDQTKVTEEENKGSGTGAAGASDTTIKPNPENTGPTLEWDNCDLREDALD
  DSVSSSSVHASPLASSPVRKNLFHFGESTTGSNFSFKSALSPSKSPAKLNQSGTSVGTDEESDVTQEEER
  DGQYFEPVVPLPDLVEVSSGEENEKVVFSHRAELYRYDKDVGQWKERGIGDIKILQNYDNKQVRIVMRRD
  QVLKLCANHTITPDMSLQNMKGTERVWVWTACDFADGERKVEHLAVRFKLQDVADSFKKIFDEAKTAQEK
  DSLITPHVSRSSTPRESPCGKIAVAVLEETTRERTDVIQGDDVADAASEVEVSSTSETTTKAVVSPPKFV
  FGSESVKRIFSSEKSKPFAFGNSSATGSLFGFSFNASLKSNNSETSSVAQSGSESKVEPKKCELSKNSDI
  EQSSDSKVKNLSASFPMEESSINYTFKTPEKEPPLWHAEFTKEELVQKLSSTTKSADHLNGLLREAEATS
  AVLMEQIKLLKSEIRRLERNQEQEESAANVEHLKNVLLQFIFLKPGSERERLLPVINTMLQLSPEEKGKL
  AAVAQGEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182588
• ORF Size: 5274 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182588.3
• RefSeq ORF: 5277 bp
• Locus ID: 285190
• UniProt ID: Q7Z3J3
• Cytogenetics: 2q12.3
• MW: 197.1 kDa