PHKA1 (NM_001122670) Human Tagged ORF Clone

CAT#: RC226362

PHKA1 (Myc-DDK-tagged)-Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 2

Product Images

Circular map for RC226362

• 

  Western blot validation of overexpression lysate (Cat# LY426544) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226362 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PHKA1 protein (Cat# TP326362). The protein was produced from HEK293T cells transfected with PHKA1 cDNA clone (Cat# RC226362) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PHKA1
• Synonyms: PHKA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226362 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAGCCGGAGTAACTCCGGGGTCCGGCTGGACGGCTACGCTCGACTGGTGCAACAGACCATCCTGT
  GCCATCAGAATCCAGTGACTGGCTTGCTTCCAGCCAGCTATGATCAGAAAGATGCTTGGGTCCGAGATAA
  TGTGTACAGCATCTTGGCTGTGTGGGGTTTGGGCCTGGCCTATCGGAAGAATGCAGACCGGGATGAGGAT
  AAGGCAAAGGCCTATGAATTGGAGCAGAGTGTAGTGAAGCTGATGAGAGGACTACTGCACTGCATGATCA
  GACAGGTGGATAAAGTAGAATCCTTCAAATATAGTCAGAGTACTAAGGATAGCCTCCATGCAAAGTACAA
  CACCAAAACCTGTGCCACTGTAGTGGGTGATGATCAATGGGGACACCTGCAGTTGGATGCTACCTCTGTG
  TACCTGCTCTTCTTAGCCCAAATGACTGCCTCAGGACTCCATATCATCCACAGCCTAGATGAAGTCAATT
  TCATACAGAACCTTGTGTTTTACATTGAAGCTGCATATAAAACTGCTGACTTCGGGATATGGGAACGTGG
  AGACAAGACCAACCAAGGGATCTCAGAGTTGAATGCCAGTTCAGTTGGAATGGCAAAGGCAGCCCTGGAA
  GCATTAGATGAACTGGATCTGTTTGGTGTGAAAGGTGGGCCTCAATCAGTTATCCATGTCCTGGCTGATG
  AAGTACAGCACTGCCAGTCTATCCTAAATTCACTACTGCCCCGTGCTTCAACATCAAAAGAGGTTGATGC
  TAGTCTACTCTCAGTGGTTTCCTTCCCTGCCTTTGCAGTAGAGGATAGCCAGTTGGTGGAGCTCACAAAA
  CAGGAAATCATCACCAAGCTTCAGGGTCGTTATGGTTGCTGTCGCTTTCTACGAGATGGATATAAAACTC
  CTAAAGAGGATCCCAATCGTCTGTACTATGAACCAGCTGAGCTGAAGCTATTTGAAAACATTGAGTGTGA
  ATGGCCATTGTTCTGGACATACTTTATTCTTGATGGGGTCTTCAGTGGCAATGCAGAACAGGTTCAAGAA
  TATAAAGAGGCTCTTGAAGCAGTCCTCATCAAGGGCAAAAATGGAGTCCCACTTCTGCCAGAGCTGTACA
  GTGTTCCTCCTGACAGGGTCGATGAAGAATATCAGAATCCTCACACTGTGGACCGAGTCCCCATGGGGAA
  ATTGCCTCACATGTGGGGTCAGTCTCTATACATTTTAGGAAGCTTGATGGCAGAGGGATTTTTAGCCCCT
  GGAGAAATTGATCCCCTGAATCGCAGGTTTTCTACTGTACCGAAGCCCGATGTTGTGGTTCAAGTCTCCA
  TTCTAGCTGAAACAGAAGAAATCAAGACCATTTTGAAGGACAAGGGAATTTACGTGGAGACCATTGCTGA
  GGTATACCCCATCAGAGTACAACCAGCTCGTATTCTCAGCCACATTTATTCCAGCCTAGGATGCAACAAT
  AGAATGAAACTCAGTGGACGACCCTACAGACACATGGGAGTGCTTGGAACTTCAAAACTCTATGACATTC
  GGAAAACTATCTTTACTTTCACTCCACAGTTTATAGACCAGCAACAGTTCTACCTGGCTCTGGACAACAA
  GATGATAGTGGAAATGCTTAGAACAGACCTCTCCTACCTCTGTAGCCGCTGGCGGATGACAGGCCAGCCC
  ACCATCACCTTCCCCATCTCACACAGCATGCTTGATGAAGATGGAACAAGCTTGAATTCAAGTATCCTGG
  CAGCACTCCGAAAAATGCAAGATGGGTATTTTGGTGGGGCAAGGGTTCAAACAGGTAAATTGTCAGAGTT
  TTTGACAACATCTTGTTGCACACACTTGAGCTTCATGGACCCTGGACCTGAGGGTAAGCTGTACAGTGAA
  GATTATGATGACAACTATGATTACCTGGAATCTGGCAACTGGATGAATGATTATGATTCAACCAGTCATG
  CTCGCTGTGGTGATGAAGTTGCTCGTTATTTAGATCACCTTTTGGCGCACACTGCTCCCCATCCTAAACT
  AGCCCCTACCTCACAGAAGGGAGGGCTAGATCGGTTCCAAGCTGCTGTGCAAACAACCTGCGACTTAATG
  TCCTTGGTGACCAAGGCCAAGGAACTGCATGTACAGAATGTTCACATGTATCTTCCTACGAAGTTATTTC
  AGGCTTCCCGGCCTTCATTCAACTTACTTGATTCACCTCATCCCCGACAGGAGAACCAGGTTCCCTCTGT
  TCGTGTAGAAATACATCTTCCTAGAGACCAGTCTGGGGAGGTGGACTTTAAAGCACTGGTTTTACAGTTG
  AAGGAGACCTCAAGCTTACAGGAACAAGCTGATATCCTCTATATGCTGTATACTATGAAAGGACCTGACT
  GGAACACTGAATTGTATAATGAACGGAGTGCTACAGTGAGAGAGCTTCTTACCGAGCTGTATGGCAAAGT
  GGGAGAAATTCGTCACTGGGGCCTGATCCGATACATTTCTGGGATCTTAAGGAAGAAAGTGGAAGCACTT
  GATGAGGCCTGCACAGACCTTCTCTCCCACCAGAAACATTTGACAGTAGGACTTCCTCCAGAACCTCGAG
  AAAAGACTATCTCTGCACCTCTGCCCTATGAGGCGCTCACTCAGCTGATAGATGAAGCCAGTGAAGGGGA
  TATGAGCATTTCAATCCTTACACAGGAAATAATGGTATATCTAGCCATGTATATGCGAACCCAGCCTGGC
  CTCTTTGCTGAAATGTTTCGACTTCGAATTGGTCTGATCATACAAGTTATGGCAACAGAACTGGCCCACT
  CCCTTCGATGCTCAGCTGAGGAAGCCACAGAGGGCCTGATGAATCTCAGTCCTTCGGCCATGAAGAATCT
  CCTGCATCACATTCTCAGCGGCAAGGAGTTTGGAGTGGAACGAAGCGTTCGTCCCACTGATTCAAATGTC
  AGTCCTGCTATTTCTATCCACGAGATTGGTGCTGTTGGAGCAACCAAAACAGAACGAACTGGGATCATGC
  AGTTAAAAAGTGAGATAAAGCAGTCACCTGGAACCTCTATGACTCCAAGTAGTGGGTCCTTTCCTAGTGC
  ATATGATCAGCAGTCATCTAAAGATAGTCGTCAAGGTCAATGGCAACGCCGAAGAAGGCTGGATGGGGCA
  CTGAATAGAGTTCCAGTTGGATTTTATCAGAAAGTATGGAAAGTTTTGCAGAAGTGTCACGGACTTTCTG
  TTGAAGGGTTTGTCCTTCCTTCCTCTACCACTAGAGAGATGACTCCAGGTGAGATTAAATTCTCTGTTCA
  TGTGGAGTCTGTCCTGAATCGTGTACCTCAGCCAGAGTACCGTCAGCTGCTGGTTGAAGCCATCCTTGTC
  CTCACCATGCTGGCAGATATTGAAATTCATAGCATCGGAAGCATCATTGCTGTGGAAAAAATAGTGCATA
  TTGCCAATGACTTGTTCCTTCAAGAACAGAAAACCCTTGGCGCAGATGATACCATGTTGGCAAAGGATCC
  CGCATCTGGCATCTGTACTCTTCTGTATGACAGTGCACCCAGTGGCAGGTTTGGCACCATGACCTACCTC
  TCCAAGGCAGCCGCCACCTACGTGCAGGAGTTCCTGCCCCACAGCATCTGTGCCATGCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226362 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRSRSNSGVRLDGYARLVQQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDED
  KAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSV
  YLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALE
  ALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSLLPRASTSKEVDASLLSVVSFPAFAVEDSQLVELTK
  QEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQE
  YKEALEAVLIKGKNGVPLLPELYSVPPDRVDEEYQNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAP
  GEIDPLNRRFSTVPKPDVVVQVSILAETEEIKTILKDKGIYVETIAEVYPIRVQPARILSHIYSSLGCNN
  RMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNKMIVEMLRTDLSYLCSRWRMTGQP
  TITFPISHSMLDEDGTSLNSSILAALRKMQDGYFGGARVQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSE
  DYDDNYDYLESGNWMNDYDSTSHARCGDEVARYLDHLLAHTAPHPKLAPTSQKGGLDRFQAAVQTTCDLM
  SLVTKAKELHVQNVHMYLPTKLFQASRPSFNLLDSPHPRQENQVPSVRVEIHLPRDQSGEVDFKALVLQL
  KETSSLQEQADILYMLYTMKGPDWNTELYNERSATVRELLTELYGKVGEIRHWGLIRYISGILRKKVEAL
  DEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEALTQLIDEASEGDMSISILTQEIMVYLAMYMRTQPG
  LFAEMFRLRIGLIIQVMATELAHSLRCSAEEATEGLMNLSPSAMKNLLHHILSGKEFGVERSVRPTDSNV
  SPAISIHEIGAVGATKTERTGIMQLKSEIKQSPGTSMTPSSGSFPSAYDQQSSKDSRQGQWQRRRRLDGA
  LNRVPVGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILV
  LTMLADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDTMLAKDPASGICTLLYDSAPSGRFGTMTYL
  SKAAATYVQEFLPHSICAMQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001122670
• ORF Size: 3630 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001122670.1, NP_001116142.1
• RefSeq Size: 6138 bp
• RefSeq ORF: 3633 bp
• Locus ID: 5255
• UniProt ID: P46020
• Cytogenetics: Xq13.1
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Insulin signaling pathway
• MW: 135.8 kDa
• Gene Summary: Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the skeletal muscle isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9D, also known as X-linked muscle glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. A pseudogene has been found on chromosome 1.[provided by RefSeq, Feb 2010]