PLEKHG4 (NM_001129731) Human Tagged ORF Clone

CAT#: RC226331

PLEKHG4 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant 5

Product Images

Circular map for RC226331

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLEKHG4
• Synonyms: ARHGEF44; PRTPHN1; SCA4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226331 representing NM_001129731
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAGGCCCCTGGAGAATGGGGATGAGTCCCCAGACTCTCAGGGCCATGCAACCGACTGGAGATTTG
  CTGTGTGCAGTTTCAGGGATGCCTGGGAAGAGGAGGAACCTGCTTCCCAGATGCACGTTAAGGACCCAGG
  TCCTCCAAGACCACCAGCCGGGGCCACCCAGGATGAGGAGCTACAGGGCAGCCCCTTGTCCAGGAAATTC
  CAGTTACCCCCAGCTGCAGATGAGTCGGGGGATGCCCAGAGGGGCACAGTAGAAAGCTCCTCAGTCCTGT
  CAGAAGGGCCAGGCCCCTCTGGAGTGGAGAGTCTCCTATGCCCCATGTCCTCCCACCTCAGCTTGGCACA
  GGGGACTCGGGATGTCCAAGGCCGGGCAGTGCTGCTTCTGTGTGCCCACAGCCCAGCCTGGCTTCAGTCT
  GAGTGCAGCAGCCAGGAACTCATCCGCCTCCTGCTGTACCTGCGAAGCATCCCCAGGCCCGAAGTACAGG
  CACTGGGACTGACAGTGCTAGTTGATGCCCGAATTTGTGCTCCAAGTTCTTCCCTCTTCTCTGGGCTCAG
  CCAACTACAAGAAGCAGCCCCAGGGGCCGTGTACCAGGTGCTGCTAGTGGGAAGCACGCTGCTGAAGGAA
  GTGCCTTCCGGGCTGCAGCTGGAGCAGTTGCCTTCTCAGAGCCTGCTGACCCACATCCCAACGGCGGGGC
  TGCCCACTTCGCTAGGAGGAGGCCTGCCTTACTGCCACCAGGCCTGGCTGGATTTCCGAAGGCGGCTGGA
  AGCTCTACTACAGAACTGCCAGGCAGCTTGTGCCCTGCTCCAGGGGGCCATCGAAAGTGTGAAGGCTGTG
  CCCCAGCCCATGGAGCCTGGGGAGGTCGGTCAGCTGCTACAGCAGACAGAGGTCCTGATGCAGCAGGTGC
  TAGACTCGCCATGGCTGGCATGGCTACAATGCCAGGGGGGCCGGGAGCTGACATGGCTGAAGCAAGAGGT
  CCCAGAGGTGACCCTGAGCCCAGACTACAGGACGGCAATGGACAAGGCTGACGAGCTATATGACCGGGTG
  GATGGATTGCTGCACCAACTGACCCTGCAGAGCAACCAGCGAATACAGGCCCTAGAGTTGGTCCAAACAC
  TGGAGGCCCGGGAAAGCGGACTGCACCAGATTGAAGTGTGGCTGCAGCAGGTGGGCTTGCCAGCACTGGA
  GGAGGCTGGGGAGCCCTCGCTGGACATGCTGCTCCAGGCCCAAGGCTCTTTTCAGGAGCTGTACCAGGTT
  GCCCAGGAGCAGGTCAGGCAAGGGGAGAAGTTTCTGCAGCCGCTGACTGGCTGGGAGGCGGCTGAACTGG
  ACCCCCCTGGGGCACGCTTTCTGGCCCTGCGAGCCCAGCTGACTGAATTCTCTAGGGCTTTGGCCCAGCG
  GTGCCAGCGGCTGGCGGATGCTGAGAGGCTGTTTCAGCTCTTCAGGGAGGCCTTGACGTGGGCTGAGGAG
  GGGCAGCGAGTGTTGGCAGAGCTGGAGCAGGAACGCCCGGGGGTTGTGTTGCAGCAGCTGCAGCTGCACT
  GGACCAGGCACCCTGACTTGCCTCCTGCCCACTTCCGAAAGATGTGGGCTCTGGCCACGGGGCTGGGCTC
  AGAGGCCATCCGCCAGGAGTGCCGCTGGGCCTGGGCGCGGTGCCAGGACACCTGGCTGGCCCTGGACCAA
  AAGCTTGAGGCTTCACTGAAGCTACCACCGGTGGGCAGCACAGCTAGCCTGTGTGTCAGCCAGGTCCCCG
  CTGCACCTGCCCACCCTCCCCTGAGGAAGGCCTACAGCTTCGATCGGAATCTGGGGCAGAGTCTCAGTGA
  ACCTGCCTGCCACTGCCACCATGCGGCCACTATTGCTGCCTGCCGCAGACCAGAGGCTGGAGGAGGTGCC
  CTGCCCCAGGCATCCCCTACTGTGCCTCCACCAGGCAGCTCTGACCCCAGGAGCCTCAACAGGCTACAGC
  TGGTGCTGGCAGAGATGGTGGCCACGGAGCGGGAGTATGTCCGGGCTCTAGAGTACACTATGGAGAACTA
  TTTCCCCGAGCTGGATCGCCCCGATGTGCCCCAGGGCCTCCGCGGTCAGCGTGCCCACCTCTTTGGCAAC
  CTGGAGAAGCTGCGGGACTTCCACTGCCACTTCTTCCTGCGTGAGCTGGAGGCTTGCACCCGGCACCCAC
  CACGAGTGGCCTATGCCTTCCTGCGCCATAGGGTGCAGTTTGGGATGTACGCGCTCTACAGCAAGAATAA
  GCCTCGCTCCGATGCCCTGATGTCAAGCTATGGGCACACCTTCTTCAAGGACAAGCAGCAAGCACTGGGG
  GACCACCTGGACCTGGCCTCCTACCTGCTAAAGCCCATCCAGCGCATGGGCAAGTACGCACTGCTGCTGC
  AGGAGCTGGCACGGGCCTGCGGGGGCCCCACGCAGGAGCTCAGTGCGCTGCGGGAGGCCCAGAGCCTTGT
  GCACTTCCAGCTGCGGCACGGAAACGACCTGCTGGCCATGGACGCCATCCAGGGCTGTGATGTTAACCTC
  AAGGAACAGGGGCAGCTGGTGCGACAGGATGAGTTTGTGGTGCGCACTGGGCGCCACAAGTCCGTGCGCC
  GCATCTTCCTTTTTGAGGAGCTGCTGCTCTTCAGCAAGCCTCGCCATGGGCCCACAGGGGTTGACACATT
  TGCCTACAAGCGCTCCTTCAAGATGGCAGACCTTGGTCTCACTGAGTGCTGTGGGAACAGCAACCTGCGC
  TTCGAGATCTGGTTCCGCGGCCGCAAGGCCAGGGACACCTTTGTGCTGCAGGCCTCCAGCCTGGCTATCA
  AGCAGGCCTGGACAGCTGACATCTCCCACCTGCTTTGGAGGCAGGCCGTCCACAACAAGGAGGTGCGCAT
  GGCTGAGATGGTGTCCATGGGTGTGGGGAACAAGGCCTTCCGAGACATTGCTCCCAGCGAGGAAGCCATC
  AACGACCGCACCGTCAACTATGTCCTGAAGTGCCGAGAAGTTCGCTCTCGGGCGTCCATTGCCGTAGCCC
  CGTTTGACCATGACAGCCTCTACCTGGGGGCCTCGAACTCCCTTCCTGGAGACCCTGCCTCTTGCTCTGT
  TCTGGGGTCCCTCAACCTGCACCTGTACAGAGACCCAGCTCTTCTGGGTCTCCGCTGTCCCCTGTATCCC
  AGCTTCCCAGAGGAAGCAGCACTGGAGGCTGAGGCAGAGCTGGGCGGCCAGCCCTCTTTGACTGCTGAGG
  ACTCAGAGATCTCGTCCCAATGCCCATCAGCCAGTGGCTCCAGTGGCTCTGACAGCAGCTGTGTGTCAGG
  GCAGGCCCTGGGTAGGGGCCTGGAGGACTTACCCTGTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226331 representing NM_001129731
  Red=Cloning site Green=Tags(s)
  
  MERPLENGDESPDSQGHATDWRFAVCSFRDAWEEEEPASQMHVKDPGPPRPPAGATQDEELQGSPLSRKF
  QLPPAADESGDAQRGTVESSSVLSEGPGPSGVESLLCPMSSHLSLAQGTRDVQGRAVLLLCAHSPAWLQS
  ECSSQELIRLLLYLRSIPRPEVQALGLTVLVDARICAPSSSLFSGLSQLQEAAPGAVYQVLLVGSTLLKE
  VPSGLQLEQLPSQSLLTHIPTAGLPTSLGGGLPYCHQAWLDFRRRLEALLQNCQAACALLQGAIESVKAV
  PQPMEPGEVGQLLQQTEVLMQQVLDSPWLAWLQCQGGRELTWLKQEVPEVTLSPDYRTAMDKADELYDRV
  DGLLHQLTLQSNQRIQALELVQTLEARESGLHQIEVWLQQVGLPALEEAGEPSLDMLLQAQGSFQELYQV
  AQEQVRQGEKFLQPLTGWEAAELDPPGARFLALRAQLTEFSRALAQRCQRLADAERLFQLFREALTWAEE
  GQRVLAELEQERPGVVLQQLQLHWTRHPDLPPAHFRKMWALATGLGSEAIRQECRWAWARCQDTWLALDQ
  KLEASLKLPPVGSTASLCVSQVPAAPAHPPLRKAYSFDRNLGQSLSEPACHCHHAATIAACRRPEAGGGA
  LPQASPTVPPPGSSDPRSLNRLQLVLAEMVATEREYVRALEYTMENYFPELDRPDVPQGLRGQRAHLFGN
  LEKLRDFHCHFFLRELEACTRHPPRVAYAFLRHRVQFGMYALYSKNKPRSDALMSSYGHTFFKDKQQALG
  DHLDLASYLLKPIQRMGKYALLLQELARACGGPTQELSALREAQSLVHFQLRHGNDLLAMDAIQGCDVNL
  KEQGQLVRQDEFVVRTGRHKSVRRIFLFEELLLFSKPRHGPTGVDTFAYKRSFKMADLGLTECCGNSNLR
  FEIWFRGRKARDTFVLQASSLAIKQAWTADISHLLWRQAVHNKEVRMAEMVSMGVGNKAFRDIAPSEEAI
  NDRTVNYVLKCREVRSRASIAVAPFDHDSLYLGASNSLPGDPASCSVLGSLNLHLYRDPALLGLRCPLYP
  SFPEEAALEAEAELGGQPSLTAEDSEISSQCPSASGSSGSDSSCVSGQALGRGLEDLPCV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001129731
• ORF Size: 3330 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001129731.3
• RefSeq ORF: 3333 bp
• Locus ID: 25894
• UniProt ID: Q58EX7
• Cytogenetics: 16q22.1
• MW: 122.7 kDa
• Gene Summary: The protein encoded by this gene can function as a guanine nucleotide exchange factor (GEF) and may play a role in intracellular signaling and cytoskeleton dynamics at the Golgi apparatus. Polymorphisms in the region of this gene have been found to be associated with spinocerebellar ataxia in some study populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]