VAV2 (NM_001134398) Human Tagged ORF Clone

CAT#: RC226230

VAV2 (Myc-DDK-tagged)-Human vav 2 guanine nucleotide exchange factor (VAV2), transcript variant 1

Product Images

Circular map for RC226230

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VAV2
• Synonyms: VAV-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226230 representing NM_001134398
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAGTGGCGGCAGTGCGGCCGCTGGCTCATCGATTGCAAGGTCCTGCCGCCCAACCACCGGGTGG
  TGTGGCCCTCGGCCGTGGTCTTCGACCTGGCGCAGGCGCTGCGCGACGGGGTCCTTCTGTGCCAGCTGCT
  GCACAACCTCTCCCCCGGCTCCATCGACCTCAAGGACATCAACTTCCGGCCGCAGATGTCCCAGTTTCTG
  TGTTTGAAGAACATACGCACCTTCCTGAAAGTCTGCCACGATAAATTTGGATTAAGGAACAGCGAGCTGT
  TTGACCCCTTTGACCTCTTCGATGTGCGAGACTTTGGAAAGGTCATCTCCGCGGTGTCGAGGCTCTCCCT
  GCACAGCATCGCGCAGAACAAAGGGATCAGGCCTTTTCCCTCAGAGGAGACCACAGAGAATGACGATGAC
  GTCTACCGCAGCCTGGAGGAGCTGGCCGACGAGCATGACCTGGGGGAGGACATCTACGACTGCGTCCCGT
  GTGAGGATGGAGGGGACGACATCTACGAGGACATCATCAAGGTGGAGGTGCAGCAGCCCATGATTAGATA
  CATGCAGAAAATGGGCATGACTGAAGATGACAAGAGGAACTGCTGCCTGCTGGAGATCCAGGAGACCGAG
  GCCAAGTACTACCGCACCCTGGAGGACATTGAGAAGAACTACATGAGCCCCCTGCGGCTGGTGCTGAGCC
  CGGCGGACATGGCAGCTGTCTTCATTAACCTGGAGGACCTGATCAAGGTGCATCACAGCTTCCTGAGGGC
  CATCGACGTGTCCGTGATGGTGGGGGGCAGCACGCTGGCCAAGGTCTTCCTCGATTTCAAGGAAAGGCTT
  CTGATCTACGGGGAGTACTGCAGCCACATGGAGCACGCCCAGAACACACTGAACCAGCTCCTGGCCAGCC
  GGGAGGACTTCAGGCAGAAAGTCGAGGAGTGCACACTGAAGGTCCAGGATGGAAAATTTAAGCTGCAAGA
  CCTGCTGGTGGTCCCCATGCAGAGGGTGCTCAAATACCACCTGCTCTTGAAGGAGCTTCTGAGCCATTCT
  GCGGAACGGCCTGAGAGGCAGCAGCTCAAAGAAGCACTGGAAGCCATGCAGGACTTGGCGATGTACATCA
  ATGAAGTTAAACGGGACAAGGAGACCTTGAGGAAAATCAGCGAATTTCAGAGTTCTATAGAAAATTTGCA
  AGTGAAACTGGAGGAATTTGGAAGACCAAAGATTGACGGGGAACTGAAAGTCCGGTCCATAGTCAACCAC
  ACCAAGCAGGACAGGTACTTGTTCCTGTTTGACAAGGTGGTCATCGTCTGCAAGCGGAAGGGCTACAGCT
  ACGAGCTCAAGGAGATCATCGAGCTGCTGTTCCACAAGATGACCGACGACCCCATGAACAACAAGGACGT
  CAAGAAGTCTCACGGGAAAATGTGGTCCTACGGCTTCTACCTAATTCACCTTCAAGGAAAGCAGGGCTTC
  CAGTTTTTCTGCAAAACAGAAGATATGAAGAGGAAGTGGATGGAGCAGTTTGAGATGGCCATGTCAAACA
  TCAAGCCAGACAAAGCCAATGCCAACCACCACAGTTTCCAGATGTACACGTTTGACAAGACCACCAACTG
  CAAAGCCTGCAAAATGTTCCTCAGGGGCACCTTCTACCAGGGATACATGTGTACCAAGTGTGGCGTCGGG
  GCACACAAGGAGTGCCTGGAAGTGATACCTCCCTGCAAGTTCACTTCTCCTGCAGATCTGGACGCCTCCG
  GAGCGGGACCAGGTCCCAAGATGGTGGCCATGCAGAATTACCATGGCAACCCAGCCCCTCCCGGGAAGCC
  TGTGCTGACCTTCCAGACGGGCGACGTGCTTGAGCTGCTGAGGGGCGACCCTGAGTCTCCGTGGTGGGAG
  GGTCGTCTGGTACAAACCAGGAAGTCAGGGTATTTCCCCAGCTCATCTGTGAAGCCCTGCCCTGTGGATG
  GAAGGCCGCCCATCAGCCGGCCGCCATCCCGGGAGATCGACTACACTGCATACCCCTGGTTTGCAGGTAA
  CATGGAGAGGCAGCAGACGGACAACCTGCTCAAGTCCCACGCCAGCGGGACCTACCTGATCAGGGAGCGG
  CCTGCCGAGGCTGAGCGCTTTGCAATAAGCATCAAGTTCAATGATGAGGTGAAGCACATCAAGGTGGTGG
  AGAAGGACAACTGGATCCACATCACAGAGGCCAAGAAATTCGACAGCCTCCTGGAGTTGGTGGAGTACTA
  CCAGTGCCACTCACTGAAGGAGAGCTTCAAGCAGCTGGACACCACACTCAAGTACCCCTACAAGTCCCGG
  GAACGTTCGGCCTCCAGGGCCTCCAGCCGGTCCCCAGCTTCCTGTGCTTCCTACAACTTTTCTTTTCTCA
  GTCCTCAGGGCCTCAGCTTTGCTTCTCAGGGCCCCTCCGCTCCCTTCTGGTCAGTGTTCACGCCCCGCGT
  CATCGGCACAGCTGTGGCCAGGTATAACTTTGCCGCCCGAGATATGAGGGAGCTTTCGCTGCGGGAGGGT
  GACGTGGTGAGGATCTACAGCCGCATCGGCGGAGACCAGGGCTGGTGGAAGGGCGAGACCAACGGACGGA
  TTGGCTGGTTTCCTTCAACGTACGTAGAAGAGGAGGGCATCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226230 representing NM_001134398
  Red=Cloning site Green=Tags(s)
  
  MEQWRQCGRWLIDCKVLPPNHRVVWPSAVVFDLAQALRDGVLLCQLLHNLSPGSIDLKDINFRPQMSQFL
  CLKNIRTFLKVCHDKFGLRNSELFDPFDLFDVRDFGKVISAVSRLSLHSIAQNKGIRPFPSEETTENDDD
  VYRSLEELADEHDLGEDIYDCVPCEDGGDDIYEDIIKVEVQQPMIRYMQKMGMTEDDKRNCCLLEIQETE
  AKYYRTLEDIEKNYMSPLRLVLSPADMAAVFINLEDLIKVHHSFLRAIDVSVMVGGSTLAKVFLDFKERL
  LIYGEYCSHMEHAQNTLNQLLASREDFRQKVEECTLKVQDGKFKLQDLLVVPMQRVLKYHLLLKELLSHS
  AERPERQQLKEALEAMQDLAMYINEVKRDKETLRKISEFQSSIENLQVKLEEFGRPKIDGELKVRSIVNH
  TKQDRYLFLFDKVVIVCKRKGYSYELKEIIELLFHKMTDDPMNNKDVKKSHGKMWSYGFYLIHLQGKQGF
  QFFCKTEDMKRKWMEQFEMAMSNIKPDKANANHHSFQMYTFDKTTNCKACKMFLRGTFYQGYMCTKCGVG
  AHKECLEVIPPCKFTSPADLDASGAGPGPKMVAMQNYHGNPAPPGKPVLTFQTGDVLELLRGDPESPWWE
  GRLVQTRKSGYFPSSSVKPCPVDGRPPISRPPSREIDYTAYPWFAGNMERQQTDNLLKSHASGTYLIRER
  PAEAERFAISIKFNDEVKHIKVVEKDNWIHITEAKKFDSLLELVEYYQCHSLKESFKQLDTTLKYPYKSR
  ERSASRASSRSPASCASYNFSFLSPQGLSFASQGPSAPFWSVFTPRVIGTAVARYNFAARDMRELSLREG
  DVVRIYSRIGGDQGWWKGETNGRIGWFPSTYVEEEGIQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001134398
• ORF Size: 2634 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134398.2
• RefSeq ORF: 2637 bp
• Locus ID: 7410
• UniProt ID: P52735
• Cytogenetics: 9q34.2
• Protein Families: Druggable Genome
• Protein Pathways: B cell receptor signaling pathway, Chemokine signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity, Regulation of actin cytoskeleton, T cell receptor signaling pathway
• MW: 101.1 kDa
• Gene Summary: VAV2 is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]