ZBTB10 (NM_001105539) Human Tagged ORF Clone

CAT#: RC226224

ZBTB10 (Myc-DDK-tagged)-Human zinc finger and BTB domain containing 10 (ZBTB10), transcript variant 1

Product Images

Circular map for RC226224

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  Western blot validation of overexpression lysate (Cat# LY426258) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226224 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ZBTB10 protein (Cat# TP326224). The protein was produced from HEK293T cells transfected with ZBTB10 cDNA clone (Cat# RC226224) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZBTB10
• Synonyms: RINZF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226224 representing NM_001105539
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTTCAGTGAAATGAACCGCAGGACGCTGGCGTTCCGAGGAGGCGGGTTGGTCACCGCTAGCGGCG
  GCGGCTCCACGAACAATAACGCTGGCGGGGAGGCCTCAGCTTGGCCTCCGCAGCCCCAGCCGAGACAGCC
  CCCGCCGCCAGCGCCGCCCGCGCTTCAGCCGCCTAATGGGCGGGGGGCCGACGAGGAAGTGGAATTGGAG
  GGCCTGGAGCCCCAAGACCTGGAGGCCTCCGCCGGGCCGGCCGCCGGCGCCGCCGAGGAAGCCAAGGAGT
  TGCTGCTCCCCCAAGACGCGGGCGGCCCCACCTCGCTTGGCGGTGGCGCGGGGGGCCCCCTGCTAGCGGA
  AAGGAACCGTCGGACTCTGGCCTTCCGAGGCGGCGGCGGCGGGGGTCTCGGCAACAATGGCAGTAGCCGC
  GGCCGCCCCGAGACCTCGGTGTGGCCCTTGAGGCATTTCAATGGGCGAGGGCCGGCGACTGTGGATCTGG
  AGCTGGACGCGCTGGAGGGGAAGGAGTTGATGCAGGACGGCGCGTCCCTGAGCGACAGCACCGAGGACGA
  GGAGGAGGGGGCGAGCCTGGGCGACGGCAGCGGGGCGGAAGGCGGCAGCTGCAGCAGCAGCAGGCGGTCG
  GGCGGCGATGGCGGGGACGAAGTGGAGGGCAGCGGTGTGGGAGCTGGCGAAGGAGAGACTGTCCAGCACT
  TCCCGCTCGCGCGGCCCAAGTCTCTAATGCAGAAGCTCCAATGCTCCTTCCAGACCTCCTGGCTCAAGGA
  CTTTCCCTGGCTGCGCTATTCCAAGGATACTGGTCTTATGTCTTGCGGCTGGTGCCAAAAGACCCCTGCA
  GATGGGGGAAGCGTGGACCTTCCCCCAGTGGGGCATGATGAGCTTTCGCGAGGGACCCGCAACTACAAGA
  AAACCCTCCTCCTGAGGCACCACGTCTCTACCGAGCACAAACTCCACGAAGCCAACGCCCAGGAGTCAGA
  AATACCATCAGAGGAGGGGTACTGTGACTTTAATAGTAGGCCAAATGAGAACTCTTATTGCTATCAACTT
  CTGCGACAACTAAATGAACAGAGAAAGAAAGGTATTCTTTGTGATGTCAGCATTGTGGTAAGCGGAAAAA
  TCTTCAAAGCTCATAAGAACATCCTGGTTGCAGGCAGCCGTTTCTTTAAGACTTTATATTGCTTTTCAAA
  CAAAGAAAGCCCTAACCAAAACAATACTACCCACTTAGATATTGCTGCAGTTCAAGGTTTTTCAGTCATC
  TTGGACTTCTTGTATTCTGGTAACCTGGTGCTCACAAGCCAAAATGCCATTGAAGTTATGACCGTGGCCA
  GCTATCTTCAAATGAGTGAAGTTGTTCAAACTTGCCGAAATTTCATTAAAGATGCCTTAAATATAAGCAT
  TAAATCAGAAGCTCCAGAGTCTGTAGTTGTGGACTATAATAATAGAAAACCAGTTAATAGAGATGGTCTG
  TCTTCATCACGGGATCAAAAAATTGCCAGTTTTTGGGCAACACGGAATCTTACCAATTTGGCAAGTAATG
  TAAAGATTGAAAATGATGGTTGTAATGTCGACGAGGGCCAAATAGAAAACTACCAAATGAATGACAGTAG
  TTGGGTCCAGGATGGATCTCCTGAAATGGCTGAAAATGAATCTGAAGGTCAAACAAAAGTGTTTATTTGG
  AATAATATGGGCTCCCAGGGAATTCAAGAGACTGGCAAAACAAGGAGGAAAAACCAAACTACAAAAAGAT
  TTATTTATAATATTCCACCTAATAATGAAACGAATTTAGAAGATTGCTCAGTAATGCAGCCACCTGTTGC
  CTATCCAGAAGAAAATACACTACTCATCAAGGAAGAACCAGATTTAGATGGTGCTCTACTCTCGGGGCCA
  GATGGTGATAGGAATGTGAATGCAAATTTATTGGCTGAAGCTGGCACTAGTCAAGATGGAGGTGATGCTG
  GTACTTCACATGATTTCAAGTATGGTTTGATGCCTGGTCCTTCAAATGATTTCAAGTATGGATTGATACC
  AGGTACTTCAAATGATTTCAAGTATGGATTGATACCAGGTGCTTCAAATGATTTCAAGTATGGATTATTG
  CCAGAATCTTGGCCAAAACAAGAAACCTGGGAAAATGGTGAATCATCTCTAATCATGAACAAGTTAAAAT
  GCCCTCATTGTAGCTATGTAGCCAAATACAGACGAACACTAAAAAGGCACTTGCTCATTCACACAGGAGT
  GAGATCATTTAGCTGTGATATTTGTGGAAAACTGTTTACTCGAAGAGAACATGTAAAAAGACATTCCCTG
  GTGCATAAAAAGGATAAAAAATACAAATGTATGGTGTGTAAGAAGATCTTCATGTTAGCAGCCAGTGTTG
  GAATAAGACATGGATCTCGACGTTATGGTGTTTGTGTAGACTGTGCAGATAAATCACAGCCAGGAGGGCA
  AGAAGGTGTAGATCAGGGACAGGATACAGAATTCCCTCGGGATGAAGAATACGAGGAGAATGAAGTAGGA
  GAAGCTGATGAAGAGCTAGTTGATGATGGAGAAGATCAGAATGATCCCTCTCGATGGGATGAATCAGGAG
  AAGTTTGTATGTCTCTAGATGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226224 representing NM_001105539
  Red=Cloning site Green=Tags(s)
  
  MSFSEMNRRTLAFRGGGLVTASGGGSTNNNAGGEASAWPPQPQPRQPPPPAPPALQPPNGRGADEEVELE
  GLEPQDLEASAGPAAGAAEEAKELLLPQDAGGPTSLGGGAGGPLLAERNRRTLAFRGGGGGGLGNNGSSR
  GRPETSVWPLRHFNGRGPATVDLELDALEGKELMQDGASLSDSTEDEEEGASLGDGSGAEGGSCSSSRRS
  GGDGGDEVEGSGVGAGEGETVQHFPLARPKSLMQKLQCSFQTSWLKDFPWLRYSKDTGLMSCGWCQKTPA
  DGGSVDLPPVGHDELSRGTRNYKKTLLLRHHVSTEHKLHEANAQESEIPSEEGYCDFNSRPNENSYCYQL
  LRQLNEQRKKGILCDVSIVVSGKIFKAHKNILVAGSRFFKTLYCFSNKESPNQNNTTHLDIAAVQGFSVI
  LDFLYSGNLVLTSQNAIEVMTVASYLQMSEVVQTCRNFIKDALNISIKSEAPESVVVDYNNRKPVNRDGL
  SSSRDQKIASFWATRNLTNLASNVKIENDGCNVDEGQIENYQMNDSSWVQDGSPEMAENESEGQTKVFIW
  NNMGSQGIQETGKTRRKNQTTKRFIYNIPPNNETNLEDCSVMQPPVAYPEENTLLIKEEPDLDGALLSGP
  DGDRNVNANLLAEAGTSQDGGDAGTSHDFKYGLMPGPSNDFKYGLIPGTSNDFKYGLIPGASNDFKYGLL
  PESWPKQETWENGESSLIMNKLKCPHCSYVAKYRRTLKRHLLIHTGVRSFSCDICGKLFTRREHVKRHSL
  VHKKDKKYKCMVCKKIFMLAASVGIRHGSRRYGVCVDCADKSQPGGQEGVDQGQDTEFPRDEEYEENEVG
  EADEELVDDGEDQNDPSRWDESGEVCMSLDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001105539
• ORF Size: 2613 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001105539.3
• RefSeq ORF: 2616 bp
• Locus ID: 65986
• UniProt ID: Q96DT7
• Cytogenetics: 8q21.13
• Protein Families: Transcription Factors
• MW: 94.7 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]