VPS53 (NM_001128159) Human Tagged ORF Clone

CAT#: RC226200

VPS53 (Myc-DDK-tagged)-Human vacuolar protein sorting 53 homolog (S. cerevisiae) (VPS53), transcript variant 1

Product Images

Circular map for RC226200

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  Western blot validation of overexpression lysate (Cat# LY426901) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226200 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified VPS53 protein (Cat# TP326200). The protein was produced from HEK293T cells transfected with VPS53 cDNA clone (Cat# RC226200) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VPS53
• Synonyms: HCCS1; hVps53L; PCH2E; pp13624
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226200 representing NM_001128159
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAGGAGGAGGAACTGGAGTTCGTGGAGGAGCTGGAAGCCGTGCTGCAGCTCACGCCCGAGGTGC
  AGCTGGCCATCGAGCAGGTGTTTCCAAGCCAGGACCCTCTAGATCGAGCAGATTTCAATGCTGTTGAGTA
  TATCAATACCCTGTTCCCAACCGAGCAATCTCTGGCGAACATAGACGAAGTCGTGAACAAAATTAGGCTG
  AAAATAAGGAGACTGGATGACAATATTCGAACTGTTGTAAGAGGTCAGACGAACGTGGGGCAGGATGGAC
  GGCAAGCGCTTGAAGAGGCTCAGAAAGCTATCCAACAACTCTTTGGCAAAATCAAAGATATCAAAGACAA
  AGCTGAAAAATCAGAGCAAATGGTGAAAGAAATCACCCGTGATATTAAGCAATTAGATCACGCCAAACGC
  CACCTGACCACCTCAATCACCACACTGAACCACCTGCACATGCTGGCAGGAGGTGTCGACTCCCTCGAAG
  CCATGACCAGGCGAAGACAATACGGAGAAGTTGCTAATCTCCTTCAGGGTGTGATGAATGTCCTGGAGCA
  CTTCCACAAGTATATGGGGATTCCGCAGATCCGGCAGCTTTCCGAAAGAGTGAAGGCTGCACAGACTGAG
  TTAGGACAGCAAATCCTGGCAGATTTTGAAGAAGCGTTTCCTTCCCAGGGCACCAAGAGACCAGGAGGAC
  CCAGCAATGTTCTACGAGATGCATGTCTGGTTGCTAATATTCTAGATCCCAGGATCAAACAGGAAATCAT
  CAAAAAGTTTATTAAACAGCATCTGTCAGAGTATCTGGTACTTTTTCAAGAAAACCAAGATGTTGCCTGG
  CTGGACAAAATCGACAGACGCTATGCCTGGATAAAACGCCAGCTTGTGGACTATGAGGAGAAATACGGCC
  GCATGTTTCCACGTGAGTGGTGCATGGCTGAGAGGATTGCGGTGGAATTTTGCCATGTGACAAGGGCAGA
  ACTTGCCAAGATTATGCGTACCAGAGCGAAGGAAATTGAAGTGAAATTGCTTCTTTTTGCTATTCAAAGA
  ACAACTAACTTTGAGGGGTTTCTTGCAAAACGCTTCTCCGGCTGCACCCTGACCGATGGGACCCTGAAAA
  AGCTTGAGTCTCCACCCCCATCTACCAATCCCTTCCTGGAAGATGAGCCAACACCAGAGATGGAGGAACT
  GGCAACGGAGAAAGGAGATTTAGATCAACCAAAGAAGCCTAAAGCCCCAGACAATCCATTTCATGGCATT
  GTTTCCAAGTGTTTTGAGCCTCATCTCTACGTGTATATCGAATCCCAAGACAAGAACCTCGGAGAGCTGA
  TAGATCGGTTTGTGGCTGATTTCAAAGCCCAGGGGCCACCTAAGCCCAACACTGATGAAGGGGGTGCCGT
  GCTCCCCAGCTGCGCCGACCTCTTTGTCTACTACAAGAAGTGCATGGTGCAATGCTCTCAGCTCAGTACT
  GGGGAGCCCATGATCGCCCTGACCACCATTTTCCAGAAGTACCTCCGAGAATACGCCTGGAAAATCCTCT
  CTGGCAACCTGCCCAAAACCACAACCAGCAGTGGAGGACTGACTATCAGCAGCCTCCTCAAGGAAAAGGA
  GGGCTCAGAAGTAGCCAAGTTCACTCTGGAGGAGCTCTGCCTCATCTGTAACATCCTGAGCACGGCAGAG
  TACTGTCTGGCCACCACCCAGCAGCTAGAAGAAAAACTCAAAGAAAAAGTGGATGTAAGTCTGATTGAAC
  GAATCAATCTGACTGGAGAGATGGACACGTTCAGCACCGTCATCTCCAGCAGTATTCAGCTGCTGGTTCA
  GGATCTGGATGCTGCCTGTGATCCTGCCCTGACTGCCATGAGCAAGATGCAGTGGCAGAACGTGGAGCAC
  GTTGGTGACCAGAGCCCCTACGTCACCTCTGTCATTCTGCACATCAAGCAGAACGTCCCCATCATCCGTG
  ACAACCTGGCTTCCACACGCAAGTACTTCACTCAGTTCTGCGTTAAATTTGCAAACTCCTTCATTCCCAA
  ATTCATCACCCACCTCTTCAAGTGCAAGCCAATTAGCATGGTGGGAGCAGAACAGCTGCTGCTGGACACC
  CACTCGCTGAAGATGGTCCTGCTCGATCTCCCCTCCATCAGCTCGCAGGTGGTGAGGAAGGCACCCGCCA
  GCTACACCAAGATCGTTGTCAAAGGCATGACCCGGGCTGAGATGATCCTCAAGGTAGTGATGGCCCCTCA
  TGAACCGTTGGTGGTGTTTGTTGACAACTACATCAAACTTCTCACAGACTGCAACACAGAAACCTTTCAG
  AAGATACTGGACATGAAGGGGCTGAAGAGGAGTGAGCAGAGCAGCATGCTGGAACTCCTGCGCCAGCGGC
  TCCCCGCACCGCCCTCGGGGGCAGAAAGCTCCGGCTCACTGTCCCTGACGGCGCCAACACCAGAGCAAGA
  GTCGTCACGCATCCGCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226200 representing NM_001128159
  Red=Cloning site Green=Tags(s)
  
  MMEEEELEFVEELEAVLQLTPEVQLAIEQVFPSQDPLDRADFNAVEYINTLFPTEQSLANIDEVVNKIRL
  KIRRLDDNIRTVVRGQTNVGQDGRQALEEAQKAIQQLFGKIKDIKDKAEKSEQMVKEITRDIKQLDHAKR
  HLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTE
  LGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANILDPRIKQEIIKKFIKQHLSEYLVLFQENQDVAW
  LDKIDRRYAWIKRQLVDYEEKYGRMFPREWCMAERIAVEFCHVTRAELAKIMRTRAKEIEVKLLLFAIQR
  TTNFEGFLAKRFSGCTLTDGTLKKLESPPPSTNPFLEDEPTPEMEELATEKGDLDQPKKPKAPDNPFHGI
  VSKCFEPHLYVYIESQDKNLGELIDRFVADFKAQGPPKPNTDEGGAVLPSCADLFVYYKKCMVQCSQLST
  GEPMIALTTIFQKYLREYAWKILSGNLPKTTTSSGGLTISSLLKEKEGSEVAKFTLEELCLICNILSTAE
  YCLATTQQLEEKLKEKVDVSLIERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALTAMSKMQWQNVEH
  VGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCVKFANSFIPKFITHLFKCKPISMVGAEQLLLDT
  HSLKMVLLDLPSISSQVVRKAPASYTKIVVKGMTRAEMILKVVMAPHEPLVVFVDNYIKLLTDCNTETFQ
  KILDMKGLKRSEQSSMLELLRQRLPAPPSGAESSGSLSLTAPTPEQESSRIRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001128159
• ORF Size: 2469 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq ORF: 2499 bp
• Locus ID: 55275
• UniProt ID: Q5VIR6
• Cytogenetics: 17p13.3
• MW: 94.2 kDa
• Gene Summary: This gene encodes a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi. [provided by RefSeq, Jul 2008]