ENOX1 (NM_001127615) Human Tagged ORF Clone

CAT#: RC226055

ENOX1 (Myc-DDK-tagged)-Human ecto-NOX disulfide-thiol exchanger 1 (ENOX1), transcript variant 2

Product Images

Circular map for RC226055

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  Western blot validation of overexpression lysate (Cat# LY426824) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226055 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ENOX1 protein (Cat# TP326055). The protein was produced from HEK293T cells transfected with ENOX1 cDNA clone (Cat# RC226055) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ENOX1
• Synonyms: bA64J21.1; cCNOX; CNOX; PIG38
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226055 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAGATGCAGGTGGAGTTGAGAACATCACCCAGCTTCCCCAGGAGCTTCCTCAGATGATGGCTGCAG
  CAGCCGATGGTTTGGGGAGTATAGCGATAGACACGACCCAGCTCAACATGTCCGTGACAGATCCCACAGC
  CTGGGCTACAGCCATGAATAACCTGGGCATGGTTCCCGTAGGGTTGCCTGGACAGCAGCTCGTGTCTGAC
  TCAATCTGTGTCCCAGGCTTTGATCCAAGCCTCAACATGATGACTGGAATCACCCCCATTAACCCAATGA
  TACCAGGCCTTGGACTGGTACCTCCCCCACCACCAACAGAAGTGGCTGTTGTCAAAGAAATAATCCACTG
  CAAAAGCTGTACTCTTTTTCCTCAAAATCCAAATCTTCCACCTCCTTCCACAAGAGAACGACCTCCTGGG
  TGTAAGACCGTGTTTGTCGGAGGATTACCAGAAAATGCTACTGAGGAAATTATTCAAGAAGTCTTTGAAC
  AGTGCGGTGATATTACAGCAATTCGGAAAAGCAAGAAGAATTTTTGTCACATTCGCTTTGCAGAGGAATT
  CATGGTTGATAAAGCCATTTACCTTTCTGGTTATAGGATGCGATTAGGGTCTAGCACCGACAAAAAGGAT
  TCAGGCCGCCTTCATGTGGACTTTGCCCAGGCCAGGGATGACTTCTATGAGTGGGAATGCAAGCAGAGGA
  TGCGTGCCCGGGAGGAGCGGCACCGGCGCAAGCTGGAGGAGGACCGGCTCAGGCCCCCATCCCCGCCTGC
  CATAATGCACTACTCGGAGCACGAAGCCGCTCTGCTGGCTGAAAAGCTGAAAGATGATAGCAAGTTTTCA
  GAGGCTATCACAGTGCTGCTTTCCTGGATTGAACGAGGGGAAGTGAATCGGCGCTCTGCAAACCAGTTCT
  ATTCCATGGTGCAGTCGGCCAACAGCCACGTCCGCCGGCTAATGAATGAAAAAGCCACCCATGAGCAAGA
  GATGGAGGAAGCCAAGGAGAATTTTAAAAATGCCTTAACTGGGATTCTCACTCAATTTGAGCAGATTGTG
  GCCGTTTTCAACGCTTCTACCAGACAAAAAGCTTGGGACCATTTCTCGAAAGCCCAGCGCAAGAACATAG
  ACATTTGGCGAAAGCATTCTGAGGAGCTCCGGAATGCTCAAAGTGAGCAGCTCATGGGCATCCGCCGCGA
  AGAAGAAATGGAAATGTCTGATGATGAGAACTGTGACAGCCCTACAAAGAAAATGAGAGTCGATGAATCA
  GCCCTGGCTGCCCAGGCCTACGCTCTGAAAGAGGAGAATGACAGTCTCCGCTGGCAGCTGGATGCCTACA
  GGAATGAGGTGGAGCTGCTGAAACAAGAAAAAGAACAGCTTTTCCGAACAGAAGAAAACCTCACCAAGGA
  CCAGCAACTGCAGTTTCTGCAGCAAACCATGCAAGGCATGCAGCAGCAATTGCTAACCATCCAGGAGGAG
  TTAAACAACAAAAAGTCAGAATTGGAACAAGCAAAGGAAGAGCAGTCCCATACACAAGCGTTACTAAAAG
  TCCTGCAGGAACAATTAAAAGGTACCAAGGAATTGGTCGAGACCAATGGCCACAGCCATGAGGATTCAAA
  TGAAATCAATGTGTTGACAGTTGCATTAGTCAACCAAGACCGAGAGAACAATATTGAGAAAAGAAGCCAA
  GGCTTAAAATCAGAGAAAGAAGCTCTGCTAATAGGTATCATATCAACGTTTCTTCACGTCCATCCTTTTG
  GAGCCAACATAGAATATCTTTGGTCATACATGCAGCAGCTGGACTCCAAGATATCTGCAAATGAAATAGA
  AATGCTTTTGATGAGGCTGCCACGCATGTTCAAACAGGAATTCACGGGTGTGGGAGCCACGCTGGAAAAA
  AGATGGAAGTTGTGTGCCTTTGAAGGAATTAAAACTACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226055 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVDAGGVENITQLPQELPQMMAAAADGLGSIAIDTTQLNMSVTDPTAWATAMNNLGMVPVGLPGQQLVSD
  SICVPGFDPSLNMMTGITPINPMIPGLGLVPPPPPTEVAVVKEIIHCKSCTLFPQNPNLPPPSTRERPPG
  CKTVFVGGLPENATEEIIQEVFEQCGDITAIRKSKKNFCHIRFAEEFMVDKAIYLSGYRMRLGSSTDKKD
  SGRLHVDFAQARDDFYEWECKQRMRAREERHRRKLEEDRLRPPSPPAIMHYSEHEAALLAEKLKDDSKFS
  EAITVLLSWIERGEVNRRSANQFYSMVQSANSHVRRLMNEKATHEQEMEEAKENFKNALTGILTQFEQIV
  AVFNASTRQKAWDHFSKAQRKNIDIWRKHSEELRNAQSEQLMGIRREEEMEMSDDENCDSPTKKMRVDES
  ALAAQAYALKEENDSLRWQLDAYRNEVELLKQEKEQLFRTEENLTKDQQLQFLQQTMQGMQQQLLTIQEE
  LNNKKSELEQAKEEQSHTQALLKVLQEQLKGTKELVETNGHSHEDSNEINVLTVALVNQDRENNIEKRSQ
  GLKSEKEALLIGIISTFLHVHPFGANIEYLWSYMQQLDSKISANEIEMLLMRLPRMFKQEFTGVGATLEK
  RWKLCAFEGIKTT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001127615
• ORF Size: 1929 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127615.2
• RefSeq Size: 2706 bp
• RefSeq ORF: 1932 bp
• Locus ID: 55068
• UniProt ID: Q8TC92
• Cytogenetics: 13q14.11
• MW: 73.3 kDa
• Gene Summary: The protein encoded by this gene is involved in plasma membrane electron transport pathways. The encoded protein has both a hydroquinone (NADH) oxidase activity and a protein disulfide-thiol interchange activity. The two activities cycle with a periodicity of 24 minutes, with one activity being at its peak when the other is at its lowest. [provided by RefSeq, Dec 2016]