ZNF195 (NM_001130520) Human Tagged ORF Clone

CAT#: RC226042

ZNF195 (Myc-DDK-tagged)-Human zinc finger protein 195 (ZNF195), transcript variant 1. Note: ORF is codon optimized

Product Images

Circular map for RC226042

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF195
• Synonyms: HRF1; ZNFP104
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226042 ORF sequence, codon optimized.
  Due to the complexity of NM_001130520, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTTGCTGACCTTTCGGGATGTCGCCATTGAATTCAGTCTGGAAGAATGGAAGTGCCTGGATCTGG
  CTCAGCAAAACTTGTACAGGGATGTAATGCTGGAAAATTACCGCAACCTGTTCAGTGTCGGGCTGACCGT
  GTGTAAGCCAGGGCTCATTACATGTCTTGAACAGCGGAAGGAGCCCTGGAATGTGAAACGCCAAGAGGCT
  GCCGACGGGCATCCAGAGATGGGCTTCCATCACGCAACCCAGGCTTGTCTCGAACTCCTGGGCTCATCTG
  ACCTGCCCGCTAGCGCGTCTCAGTCTGCTGGAATCACGGGGGTGAACCATCGGGCTCAGCCTGGCCTGAA
  CGTTAGTGTAGACAAATTCACCGCCTTGTGTAGCCCTGGGGTCCTGCAAACTGTGAAGTGGTTTCTGGAG
  TTTCGCTGTATTTTTAGTCTGGCCATGAGCTCACACTTCACTCAGGACCTCTTGCCTGAGCAGGGAATTC
  AGGACGCATTTCCTAAGCGGATCCTCCGGGGTTACGGGAATTGTGGGCTGGACAACCTTTACCTGCGCAA
  GGATTGGGAATCTTTGGACGAATGTAAGCTGCAGAAGGATTACAACGGCCTGAACCAGTGCTCTTCTACC
  ACTCATAGTAAGATCTTCCAGTATAACAAGTACGTAAAAATCTTCGATAACTTTTCTAACTTGCACCGCA
  GAAATATATCCAACACCGGTGAGAAGCCCTTTAAATGTCAGGAATGCGGGAAGTCATTTCAGATGCTCAG
  TTTTCTCACAGAACACCAAAAAATCCACACCGGCAAGAAGTTTCAGAAGTGCGGTGAGTGCGGGAAGACC
  TTTATCCAGTGCTCCCATTTTACTGAGCCTGAAAATATTGACACAGGTGAGAAGCCATATAAATGTCAGG
  AGTGCAATAATGTCATCAAGACCTGTTCTGTGCTGACCAAGAACAGGATTTACGCAGGAGGAGAACACTA
  CCGCTGTGAGGAGTTCGGGAAAGTGTTTAACCAGTGCAGCCACCTTACGGAACACGAGCATGGCACTGAG
  GAAAAGCCCTGCAAGTATGAAGAGTGCAGCAGTGTTTTCATCTCCTGTTCATCCCTGAGTAACCAGCAGA
  TGATCCTGGCCGGCGAGAAATTGAGCAAATGCGAAACCTGGTACAAAGGATTCAACCACTCACCTAATCC
  TTCTAAACATCAAAGAAATGAGATAGGAGGAAAACCTTTCAAGTGTGAGGAATGTGATTCCATTTTTAAA
  TGGTTTAGTGACCTGACTAAGCACAAGCGGATTCACACTGGCGAGAAGCCATACAAGTGTGATGAGTGCG
  GGAAGGCCTACACTCAAAGTTCTCATCTCTCAGAACATCGCCGCATTCACACCGGCGAGAAGCCCTACCA
  GTGTGAGGAATGCGGCAAGGTATTCAGGACCTGTAGCAGCCTCTCAAATCACAAGAGGACCCACAGTGAG
  GAGAAGCCATATACCTGTGAGGAGTGCGGAAACATATTTAAGCAGCTTAGCGACCTGACCAAACACAAGA
  AGACCCATACAGGAGAGAAACCTTACAAGTGCGACGAATGCGGAAAAAACTTCACACAGTCATCCAATCT
  GATCGTTCACAAGAGAATACACACTGGCGAGAAGCCCTACAAGTGCGAAGAGTGTGGTAGGGTATTCATG
  TGGTTCTCCGACATAACAAAGCACAAGAAGACTCATACTGGAGAGAAACCCTATAAGTGTGACGAGTGCG
  GTAAAAACTTTACGCAGAGTTCCAATCTGATCGTGCATAAGCGCATACATACCGGAGAGAAACCATACAA
  GTGCGAGAAATGCGGAAAAGCCTTTACCCAGTTTAGCCACCTGACAGTGCATGAGAGCATCCATACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226042 representing NM_001130520
  Red=Cloning site Green=Tags(s)
  
  MTLLTFRDVAIEFSLEEWKCLDLAQQNLYRDVMLENYRNLFSVGLTVCKPGLITCLEQRKEPWNVKRQEA
  ADGHPEMGFHHATQACLELLGSSDLPASASQSAGITGVNHRAQPGLNVSVDKFTALCSPGVLQTVKWFLE
  FRCIFSLAMSSHFTQDLLPEQGIQDAFPKRILRGYGNCGLDNLYLRKDWESLDECKLQKDYNGLNQCSST
  THSKIFQYNKYVKIFDNFSNLHRRNISNTGEKPFKCQECGKSFQMLSFLTEHQKIHTGKKFQKCGECGKT
  FIQCSHFTEPENIDTGEKPYKCQECNNVIKTCSVLTKNRIYAGGEHYRCEEFGKVFNQCSHLTEHEHGTE
  EKPCKYEECSSVFISCSSLSNQQMILAGEKLSKCETWYKGFNHSPNPSKHQRNEIGGKPFKCEECDSIFK
  WFSDLTKHKRIHTGEKPYKCDECGKAYTQSSHLSEHRRIHTGEKPYQCEECGKVFRTCSSLSNHKRTHSE
  EKPYTCEECGNIFKQLSDLTKHKKTHTGEKPYKCDECGKNFTQSSNLIVHKRIHTGEKPYKCEECGRVFM
  WFSDITKHKKTHTGEKPYKCDECGKNFTQSSNLIVHKRIHTGEKPYKCEKCGKAFTQFSHLTVHESIHT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130520
• ORF Size: 1887 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130520.1, NM_001130520.2, NP_001123992.1
• RefSeq Size: 3187 bp
• RefSeq ORF: 1890 bp
• Locus ID: 7748
• UniProt ID: O14628
• Cytogenetics: 11p15.4
• Protein Families: Transcription Factors
• MW: 72.3 kDa
• Gene Summary: This gene encodes a protein belonging to the Krueppel C2H2-type zinc-finger protein family. These family members are transcription factors that are implicated in a variety of cellular processes. This gene is located near the centromeric border of chromosome 11p15.5, next to an imprinted domain that is associated with maternal-specific loss of heterozygosity in Wilms' tumors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]