LRRC70 (NM_181506) Human Tagged ORF Clone

CAT#: RC226033

LRRC70 (Myc-DDK-tagged)-Human leucine rich repeat containing 70 (LRRC70)

Product Images

Circular map for RC226033

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LRRC70
• Synonyms: SLRN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226033 representing NM_181506
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGGATTACAGTTTTCTCTGCCTTGCCTACGACTGTTTCTGGTTGTTACCTGTTATCTTTTATTAT
  TACTCCACAAAGAAATACTTGGATGTTCGTCTGTTTGTCAGCTCTGCACTGGGAGACAAATTAACTGCCG
  TAACTTAGGCCTTTCGAGTATTCCTAAGAATTTTCCTGAAAGTACAGTTTTTCTGTATCTGACTGGGAAT
  AATATATCTTATATAAATGAAAGTGAATTAACAGGACTTCATTCTCTTGTAGCATTGTATTTGGATAATT
  CTAACATTCTGTATGTATATCCAAAAGCCTTTGTTCAATTGAGGCATCTATATTTTCTATTTCTAAATAA
  TAATTTCATCAAACGCTTAGATCCTGGAATATTTAAGGGACTTTTAAATCTTCGTAATTTATATTTACAG
  TATAATCAGGTATCTTTTGTTCCGAGAGGAGTATTTAATGATCTAGTTTCAGTTCAGTACTTAAATCTAC
  AAAGGAATCGCCTCACTGTCCTTGGGAGTGGTACCTTTGTTGGTATGGTTGCTCTTCGGATACTTGATTT
  ATCAAACAATAACATTTTGAGGATATCAGAATCAGGCTTTCAACATCTTGAAAACCTTGCTTGTTTGTAT
  TTAGGAAGTAATAATTTAACAAAAGTACCATCAAATGCCTTTGAAGTACTTAAAAGTCTTAGAAGACTTT
  CTTTGTCTCATAATCCTATTGAAGCAATACAGCCCTTTGCATTTAAAGGACTTGCCAATCTGGAATACCT
  CCTCCTGAAAAATTCAAGAATTAGGAATGTTACTAGGGATGGGTTTAGTGGAATTAATAATCTTAAACAT
  TTGATCTTAAGTCATAATGATTTAGAGAATTTAAATTCTGACACATTCAGTTTGTTAAAGAATTTAATTT
  ACCTTAAGTTAGATAGAAACAGAATAATTAGCATTGATAATGATACATTTGAAAATATGGGAGCATCTTT
  GAAGATCCTTAATCTGTCATTTAATAATCTTACAGCCTTGCATCCAAGGGTCCTTAAGCCGTTGTCTTCA
  TTGATTCATCTTCAGGCAAATTCTAATCCTTGGGAATGTAACTGCAAACTTTTGGGCCTTCGAGACTGGC
  TAGCATCTTCAGCCATTACTCTAAACATCTATTGTCAGAATCCCCCATCCATGCGTGGCAGAGCATTACG
  TTATATTAACATTACAAATTGTGTTACATCTTCAATAAATGTATCCAGAGCTTGGGCTGTTGTAAAATCT
  CCTCATATTCATCACAAGACTACTGCGCTAATGATGGCCTGGCATAAAGTAACCACAAATGGCAGTCCTC
  TGGAAAATACTGAGACTGAGAACATTACTTTCTGGGAACGAATTCCTACTTCACCTGCTGGTAGATTTTT
  TCAAGAGAATGCCTTTGGTAATCCATTAGAGACTACAGCAGTGTTACCTGTGCAAATACAACTTACTACT
  TCTGTTACCTTGAACTTGGAAAAAAACAGTGCTCTACCGAATGATGCTGCTTCAATGTCAGGGAAAACAT
  CTCTAATTTGTACACAAGAAGTTGAGAAGTTGAATGAGGCTTTTGACATTTTGCTAGCTTTTTTCATCTT
  AGCTTGTGTTTTAATCATTTTTTTGATCTACAAAGTTGTTCAGTTTAAACAAAAACTAAAGGCATCAGAA
  AACTCAAGGGAAAATAGACTTGAATACTACAGCTTTTATCAGTCAGCAAGGTATAATGTAACTGCCTCAA
  TTTGTAACACTTCCCCAAATTCTCTAGAAAGTCCTGGCTTGGAGCAGATTCGACTTCATAAACAAATTGT
  TCCTGAAAATGAGGCACAGGTCATTCTTTTTGAACATTCTGCTTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226033 representing NM_181506
  Red=Cloning site Green=Tags(s)
  
  MCGLQFSLPCLRLFLVVTCYLLLLLHKEILGCSSVCQLCTGRQINCRNLGLSSIPKNFPESTVFLYLTGN
  NISYINESELTGLHSLVALYLDNSNILYVYPKAFVQLRHLYFLFLNNNFIKRLDPGIFKGLLNLRNLYLQ
  YNQVSFVPRGVFNDLVSVQYLNLQRNRLTVLGSGTFVGMVALRILDLSNNNILRISESGFQHLENLACLY
  LGSNNLTKVPSNAFEVLKSLRRLSLSHNPIEAIQPFAFKGLANLEYLLLKNSRIRNVTRDGFSGINNLKH
  LILSHNDLENLNSDTFSLLKNLIYLKLDRNRIISIDNDTFENMGASLKILNLSFNNLTALHPRVLKPLSS
  LIHLQANSNPWECNCKLLGLRDWLASSAITLNIYCQNPPSMRGRALRYINITNCVTSSINVSRAWAVVKS
  PHIHHKTTALMMAWHKVTTNGSPLENTETENITFWERIPTSPAGRFFQENAFGNPLETTAVLPVQIQLTT
  SVTLNLEKNSALPNDAASMSGKTSLICTQEVEKLNEAFDILLAFFILACVLIIFLIYKVVQFKQKLKASE
  NSRENRLEYYSFYQSARYNVTASICNTSPNSLESPGLEQIRLHKQIVPENEAQVILFEHSAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181506
• ORF Size: 1866 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181506.5
• RefSeq ORF: 1869 bp
• Locus ID: 100130733
• UniProt ID: Q7Z2Q7
• Cytogenetics: 5q12.1
• MW: 70.1 kDa
• Gene Summary: Renders cells highly sensitive to the activation by cytokines and lipopolysaccharide (LPS).[UniProtKB/Swiss-Prot Function]