EBF4 (NM_001110514) Human Tagged ORF Clone

CAT#: RC226006

EBF4 (Myc-DDK-tagged)-Human early B-cell factor 4 (EBF4)

Product Images

Circular map for RC226006

• 

  Western blot validation of overexpression lysate (Cat# LY426327) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226006 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified EBF4 protein (Cat# TP326006). The protein was produced from HEK293T cells transfected with EBF4 cDNA clone (Cat# RC226006) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EBF4
• Synonyms: COE4; O/E-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226006 representing NM_001110514
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCTCTGCCCCGCAGCGGGCTGAACCTGAAGGAGGAGCCGCTGCTGCCCGCCGGCCTGGGCTCAG
  TGCGCTCCTGGATGCAGGGCGCGGGCATCCTGGACGCCAGCACCGCGGCGCAGAGTGGCGTGGGTCTGGC
  ACGAGCACATTTTGAGAAGCAGCCTCCCTCCAACCTCAGGAAATCCAACTTCTTCCACTTCGTGCTGGCC
  ATGTACGACCGGCAGGGGCAGCCCGTGGAGGTGGAGCGCACAGCCTTCATCGACTTCGTGGAAAAGGACC
  GAGAGCCCGGGGCGGAAAAGACTAACAATGGGATCCATTACCGCCTCCGGCTGGTGTATAACAATGGACT
  GCGGACAGAGCAAGACCTCTACGTGCGTCTCATCGACTCCATGTCCAAACAGGCCATCATCTATGAGGGG
  CAGGACAAGAACCCCGAAATGTGCCGAGTGCTGCTCACCCATGAGATCATGTGCAGCCGGTGCTGTGACC
  GGAAGAGCTGTGGCAACCGGAATGAGACGCCCTCAGACCCCGTCATCATTGACAGGTTCTTCCTCAAGTT
  CTTCCTCAAATGCAACCAGAACTGCCTGAAGAATGCGGGGAATCCCAGAGACATGCGCCGCTTCCAGGTG
  GTGGTGTCCACGACGGTGAGCGTGGACGGACACGTGCTGGCCGTGTCCGACAACATGTTTGTGCACAACA
  ACTCCAAGCATGGCCGCAGGGCGCGCCGCCTGGACCCCTCCGAAGCTGCCACCCCCTGCATCAAGGCCAT
  CAGCCCCGGGGAGGGCTGGACCACGGGCGGCGCCACCGTCATTGTCATCGGCGACAACTTCTTCGACGGG
  TTGCAGGTCGTGTTCGGAAACGTGCTCGTGTGGAGCGAGCTCATCACGCCCCACGCCATCCGGGTGCAGA
  CGCCCCCGCGGCACATCCCCGGGGTGGTGGAGGTGACCCTCTCCTACAAGTCCAAGCAGTTTTGCAAGGG
  ATGCCCCGGCCGCTTTGTCTACACAGCTCTGAACGAGCCCACCATTGACTACGGATTCCAGAGGCTACAG
  AAAGTCATTCCCAGACACCCCGGAGACCCCGAGAGGCTGCCCAAGGAAGTGCTGCTGAAGCGGGCGGCCG
  ACTTGGCAGAAGCCCTGTACGGAGTGCCCGGCAGTAACCAGGAGCTGCTCCTGAAGCGCGCGGCGGACGT
  GGCCGAGGCTCTGTACAGCACCCCCCGCGCACCCGGGCCGCTCGCACCCCTGGCCCCGAGCCACCCACAC
  CCCGCCGTCGTGGGCATCAACGCCTTCAGCAGCCCGCTGGCCATCGCCGTCGGGGACGCCACCCCGGGGC
  CCGAGCCGGGCTACGCGCGCAGCTGCAGCAGCGCGTCCCCCCGCGGGTTCGCGCCCAGCCCCGGCTCGCA
  GCAGAGCGGCTACGGCGGCGGCCTCGGAGCTGGCCTGGGCGGCTACGGCGCGCCGGGCGTGGCCGGCCTC
  GGCGTGCCTGGGTCCCCCAGCTTCCTCAATGGCTCCACCGCCACCTCGCCCTTCGCCATCATGCCCTCTA
  GCCCCCCGCTGGCGGCTGCCTCCTCCATGTCCCTCCCGGCCGCTGCCCCCACCACCAGCGTGTTCTCCTT
  CTCGCCTGTCAACATGATCTCCGCCGTCAAACAGAGGAGCGCCTTCGCCCCCGTGCTGCGCCCCCCAAGC
  TCCCCACCCCAGGCCTGCCCCAGAGCCCACGGAGAGGGGCTTCCAGACCAGTCTTTTGAGGATTCTGACA
  AGTTTCACTCTCCAGCCCGGGGGCTTCAGGGCCTGGCATACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226006 representing NM_001110514
  Red=Cloning site Green=Tags(s)
  
  MDALPRSGLNLKEEPLLPAGLGSVRSWMQGAGILDASTAAQSGVGLARAHFEKQPPSNLRKSNFFHFVLA
  MYDRQGQPVEVERTAFIDFVEKDREPGAEKTNNGIHYRLRLVYNNGLRTEQDLYVRLIDSMSKQAIIYEG
  QDKNPEMCRVLLTHEIMCSRCCDRKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRRFQV
  VVSTTVSVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEAATPCIKAISPGEGWTTGGATVIVIGDNFFDG
  LQVVFGNVLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGCPGRFVYTALNEPTIDYGFQRLQ
  KVIPRHPGDPERLPKEVLLKRAADLAEALYGVPGSNQELLLKRAADVAEALYSTPRAPGPLAPLAPSHPH
  PAVVGINAFSSPLAIAVGDATPGPEPGYARSCSSASPRGFAPSPGSQQSGYGGGLGAGLGGYGAPGVAGL
  GVPGSPSFLNGSTATSPFAIMPSSPPLAAASSMSLPAAAPTTSVFSFSPVNMISAVKQRSAFAPVLRPPS
  SPPQACPRAHGEGLPDQSFEDSDKFHSPARGLQGLAYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001110514
• ORF Size: 1794 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110514.1, NP_001103984.1
• RefSeq ORF: 1797 bp
• Locus ID: 57593
• UniProt ID: Q9BQW3
• Cytogenetics: 20p13
• MW: 63.8 kDa
• Gene Summary: EBF4 belongs to the conserved Olf/EBF family of helix-loop-helix transcription factors, members of which play important roles in neural development and B-cell maturation (Wang et al., 2002 [PubMed 12139918]).[supplied by OMIM, Mar 2008]