TBX22 (NM_001109878) Human Tagged ORF Clone

CAT#: RC225891

TBX22 (Myc-DDK-tagged)-Human T-box 22 (TBX22), transcript variant 1

Product Images

Circular map for RC225891

• 

  Western blot validation of overexpression lysate (Cat# LY426305) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225891 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TBX22
• Synonyms: ABERS; CLPA; CPX; dJ795G23.1; TBXX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225891 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGAGCTCTCGGGCGCGTGCCTTCTCCGTGGAAGCCTTGGTGGGGAGACCCAGCAAAAGAAAAC
  TCCAAGACCCAATACAGGCGGAGCAGCCTGAGCTGCGGGAGAAAAAGGGCGGAGAGGAAGAGGAGGAGAG
  AAGGAGCAGCGCTGCAGGGAAGAGCGAGCCGCTTGAAAAACAACCTAAGACAGAGCCCTCAACATCTGCT
  TCCTCTGGCTGCGGCAGCGACAGCGGCTACGGCAACAGCTCTGAAAGTCTGGAAGAGAAAGATATTCAAA
  TGGAGCTTCAAGGATCTGAACTGTGGAAAAGATTCCATGACATCGGGACTGAGATGATCATTACTAAAGC
  GGGCAGGCGGATGTTCCCCTCTGTTCGGGTCAAGGTGAAAGGGTTGGATCCAGGGAAGCAGTACCATGTG
  GCCATCGATGTGGTGCCGGTGGATTCCAAACGCTATAGGTACGTCTATCACAGCTCACAGTGGATGGTAG
  CTGGGAATACAGACCATTTGTGCATCATTCCTAGATTCTATGTTCACCCGGACTCACCCTGCTCGGGAGA
  GACCTGGATGCGGCAGATCATCAGCTTTGATCGCATGAAACTCACCAACAATGAGATGGATGACAAAGGC
  CACATCATTCTGCAATCCATGCATAAGTACAAACCCCGAGTGCACGTGATAGAGCAAGGCAGCAGTGTTG
  ACCTGTCCCAGATTCAGTCCTTGCCCACTGAAGGTGTTAAAACATTCTCCTTTAAAGAAACTGAGTTCAC
  CACAGTAACGGCTTACCAAAACCAACAGATTACGAAACTAAAAATAGAAAGAAATCCTTTTGCTAAAGGA
  TTTAGAGATACTGGAAGAAACAGGGGTGTATTGGATGGGCTTTTAGAGACCTACCCATGGAGGCCTTCTT
  TCACTCTCGATTTTAAAACCTTTGGCGCAGACACACAAAGTGGAAGCAGTGGCTCATCTCCAGTGACCTC
  TAGTGGAGGGGCCCCCTCTCCTTTGAACTCCTTACTTTCTCCACTTTGCTTTTCACCTATGTTTCATTTA
  CCTACAAGCTCCCTTGGAATGCCCTGTCCAGAGGCATACCTGCCCAATGTCAACCTGCCTCTATGCTACA
  AGATTTGTCCAACTAATTTTTGGCAACAGCAACCTCTTGTTTTACCGGCTCCTGAAAGACTAGCAAGCAG
  CAACAGTTCTCAGTCTTTAGCCCCACTCATGATGGAAGTGCCTATGTTATCTTCCCTGGGGGTCACCAAT
  TCAAAAAGCGGTTCATCTGAAGACTCCAGTGATCAGTATCTACAAGCACCTAATTCTACCAATCAAATGT
  TATATGGATTACAGTCACCTGGAAATATTTTTCTGCCAAACTCCATCACCCCAGAAGCACTTAGTTGCTC
  CTTTCATCCTTCCTATGACTTTTATAGATACAATTTCTCTATGCCATCTAGACTGATAAGTGGTTCCAAC
  CATCTTAAAGTGAATGACGACAGTCAAGTTTCTTTTGGAGAAGGCAAATGTAATCATGTTCATTGGTATC
  CAGCAATTAACCATTACCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225891 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALSSRARAFSVEALVGRPSKRKLQDPIQAEQPELREKKGGEEEEERRSSAAGKSEPLEKQPKTEPSTSA
  SSGCGSDSGYGNSSESLEEKDIQMELQGSELWKRFHDIGTEMIITKAGRRMFPSVRVKVKGLDPGKQYHV
  AIDVVPVDSKRYRYVYHSSQWMVAGNTDHLCIIPRFYVHPDSPCSGETWMRQIISFDRMKLTNNEMDDKG
  HIILQSMHKYKPRVHVIEQGSSVDLSQIQSLPTEGVKTFSFKETEFTTVTAYQNQQITKLKIERNPFAKG
  FRDTGRNRGVLDGLLETYPWRPSFTLDFKTFGADTQSGSSGSSPVTSSGGAPSPLNSLLSPLCFSPMFHL
  PTSSLGMPCPEAYLPNVNLPLCYKICPTNFWQQQPLVLPAPERLASSNSSQSLAPLMMEVPMLSSLGVTN
  SKSGSSEDSSDQYLQAPNSTNQMLYGLQSPGNIFLPNSITPEALSCSFHPSYDFYRYNFSMPSRLISGSN
  HLKVNDDSQVSFGEGKCNHVHWYPAINHYL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001109878
• ORF Size: 1560 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001109878.2
• RefSeq Size: 2355 bp
• RefSeq ORF: 1563 bp
• Locus ID: 50945
• UniProt ID: Q9Y458
• Cytogenetics: Xq21.1
• Protein Families: Transcription Factors
• MW: 57.9 kDa
• Gene Summary: This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. Mutations in this gene have been associated with the inherited X-linked disorder, Cleft palate with ankyloglossia, and it is believed to play a major role in human palatogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]