CYP2C18 (NM_001128925) Human Tagged ORF Clone

CAT#: RC225708

CYP2C18 (Myc-DDK-tagged)-Human cytochrome P450, family 2, subfamily C, polypeptide 18 (CYP2C18), transcript variant 2

Product Images

Circular map for RC225708

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CYP2C18
• Synonyms: CPCI; CYP2C; CYP2C17; P450-6B/29C; P450IIC17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225708 representing NM_001128925
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCAGCTGTGGCTCTGGTGCTCTGTCTCTCCTGTTTGTTTCTCCTTTCACTCTGGAGGCAGAGCT
  CTGGAAGAGGGAGGCTCCCGTCTGGCCCCACTCCTCTCCCGATTATTGGAAATATCCTGCAGTTAGATGT
  TAAGGACATGAGCAAATCCTTAACCAATTTCTCAAAAGTCTATGGCCCTGTGTTCACTGTGTATTTTGGC
  CTGAAGCCCATTGTGGTGTTGCATGGATATGAAGCAGTGAAGGAGGCCCTGATTGATCATGGAGAGGAGT
  TTTCTGGAAGAGGAAGTTTTCCAGTGGCTGAAAAAGTTAACAAAGGACTTGGAATCCTTTTCAGCAATGG
  AAAGAGATGGAAGGAGATCCGGCGTTTCTGCCTCATGACTCTGCGGAATTTTGGGATGGGGAAGAGGAGC
  ATCGAGGACCGTGTTCAAGAGGAAGCCCGCTGCCTTGTGGAGGAGTTGAGAAAAACCAATGCCTCACCCT
  GTGATCCCACTTTCATCCTGGGCTGTGCTCCCTGCAATGTGATCTGCTCTGTTATTTTCCATGATCGATT
  TGATTATAAAGATCAGAGGTTTCTTAACTTGATGGAAAAATTCAATGAAAACCTCAGGATTCTGAGCTCT
  CCATGGATCCAGGAAAAGCACAATCAACAGTCTGAATTTACTGTTGAAAGCTTGATAGCCACTGTAACTG
  ATATGTTTGGGGCTGGAACAGAGACAACGAGCACCACTCTGAGATATGGACTCCTGCTCCTGCTGAAGTA
  CCCAGAGGTCACAGCTAAAGTCCAGGAAGAGATTGAATGTGTAGTTGGCAGAAACCGGAGCCCCTGTATG
  CAGGACAGGAGTCACATGCCCTACACAGATGCTGTGGTGCACGAGATCCAGAGATACATTGACCTCCTCC
  CCACCAACCTGCCCCATGCAGTGACCTGTGATGTTAAATTCAAAAACTACCTCATCCCCAAGGGCACGAC
  CATAATAACATCCCTGACTTCTGTGCTGCACAATGACAAAGAATTCCCCAACCCAGAGATGTTTGACCCT
  GGCCACTTTCTGGATAAGAGTGGCAACTTTAAGAAAAGTGACTACTTCATGCCTTTCTCAGCAGGAAAAC
  GGATGTGTATGGGAGAGGGCCTGGCCCGCATGGAGCTGTTTTTATTCCTGACCACCATTTTGCAGAACTT
  TAACCTGAAATCTCAGGTTGACCCAAAGGATATTGACATCACCCCCATTGCCAATGCATTTGGTCGTGTG
  CCACCCTTGTACCAGCTCTGCTTCATTCCTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225708 representing NM_001128925
  Red=Cloning site Green=Tags(s)
  
  MDPAVALVLCLSCLFLLSLWRQSSGRGRLPSGPTPLPIIGNILQLDVKDMSKSLTNFSKVYGPVFTVYFG
  LKPIVVLHGYEAVKEALIDHGEEFSGRGSFPVAEKVNKGLGILFSNGKRWKEIRRFCLMTLRNFGMGKRS
  IEDRVQEEARCLVEELRKTNASPCDPTFILGCAPCNVICSVIFHDRFDYKDQRFLNLMEKFNENLRILSS
  PWIQEKHNQQSEFTVESLIATVTDMFGAGTETTSTTLRYGLLLLLKYPEVTAKVQEEIECVVGRNRSPCM
  QDRSHMPYTDAVVHEIQRYIDLLPTNLPHAVTCDVKFKNYLIPKGTTIITSLTSVLHNDKEFPNPEMFDP
  GHFLDKSGNFKKSDYFMPFSAGKRMCMGEGLARMELFLFLTTILQNFNLKSQVDPKDIDITPIANAFGRV
  PPLYQLCFIPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001128925
• ORF Size: 1293 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001128925.2
• RefSeq ORF: 1296 bp
• Locus ID: 1562
• UniProt ID: P33260
• Cytogenetics: 10q23.33
• Protein Families: Druggable Genome, P450
• Protein Pathways: Arachidonic acid metabolism, Drug metabolism - cytochrome P450, Linoleic acid metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism
• MW: 48.6 kDa
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum but its specific substrate has not yet been determined. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. An additional gene, CYP2C17, was once thought to exist; however, CYP2C17 is now considered an artefact based on a chimera of CYP2C18 and CYP2C19. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]