TEDC1 (NM_001134875) Human Tagged ORF Clone

CAT#: RC225698

C14orf80 (Myc-DDK-tagged)-Human chromosome 14 open reading frame 80 (C14orf80), transcript variant 1

Product Images

Circular map for RC225698

• 

  Western blot validation of overexpression lysate (Cat# LY427507) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225698 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TEDC1
• Synonyms: C14orf80
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225698 representing NM_001134875
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAGGCGGCGGCAGCGGGTGGACCCCGCGGCTGGGGCCCGGGCCGGGGCCCTGCCTGAGGCCATCG
  CCGCGTTGAGTCGGTCGCTGCCCTCGGGACCCAGCCCCGAGATCTTCCGCCGCGCCAAGTTCGACCGTCC
  GGAGGCGACCTCCGCGCTCTGGCAGCTCCTCTTCCGTGTGCTCTCGCCACTCCCTGCGGGCAACGCCTTG
  GCATCGCTCGCCCTGGAGGTCCAAGCCCGCTTGGTGAAGTCAGCACTATGCTCCCAGGGCTACCCGAGGC
  TGGCACTGGCACAACTACCTGAGGATGGCTCGCAGGGCAGTCGGGAGCTGCTGCTGGCTCTGTCCTGGCT
  CTTGGCCCGAGGACCTGTGCCCGAGCAGATGCTGGCCCAGGCCCGAGTGCCTCTGGGTGACGAGATGACT
  GTGTGCCAGTGTGAGGCCCTGGCCAGCCCTGGCCCACCTGCACCCCACATGGAAGCAGAGGGTCCTGTGG
  ATGTCCGCCATGTGCAGTGGCTGATGGGAAAGCTGCGGTTCCGGTGGCGCCAGCTGGTGTCCAGTCAGCA
  GGAGCAGTGCGCCCTCCTGAGCAAGATCCACCTGTACACACGCGGCTGCCACAGCGACCAGAGCCTTAGC
  CATCTGTCTGTCACTGAAGCAGAGATGCTCAGGGACCCAGAGGGAGGCCAGCAGCTGCTGCGGACTCTGG
  AGCGTGAGAACCAGCGCCTGGAGGCTGTCCTGGCGTGGCGGCGCTCTGAGCTGGTCTTCTGGCGGTGGAT
  GGACACGGTCCTGGGCACCTGTGCCCCGGAGGTGCCTGCTGCAGCCTCACAGCCCACCTTCCTGCCCTGG
  GTCCCCGAGCGCGGGGGTGGCGAGTTGGACCTGGTAGTGCGGGAGCTGCAGGCACTGGAGGAGGAGCTGC
  GGGAGGCTGCGGAGCGCAGGCGGGCGGCCTGGGAGGCCAAGGCTGGAGGCTGTGGACGGGGGCCAGAGTG
  GAGTGCCGCGCGGCGGGCCTCTCGGGAGGCTGTGGAAAAGGAGCTGGGAGCTCTACAGCAGTGCTGGGAG
  CGAGACGGTGGCCCGGCCCAGCCCCATGGGCCACACCGGCTGGTGAGACGAGAGGATGGGGCAGCAGGGG
  ACCGGGACCTGCGGGCAGCTGTGGTGATCAGGACGCTGAGGAGCCAGGAGGCCTGCCTGGAGGCGGTGCT
  ACGTCGACTACAGGGACAGTGTCGGCAGGAACTGGCCAGGCTGGTGGGAGCCCGCCCTGGTCTCATCTGG
  ATCCCGCCACCTGGACGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225698 representing NM_001134875
  Red=Cloning site Green=Tags(s)
  
  MGRRRQRVDPAAGARAGALPEAIAALSRSLPSGPSPEIFRRAKFDRPEATSALWQLLFRVLSPLPAGNAL
  ASLALEVQARLVKSALCSQGYPRLALAQLPEDGSQGSRELLLALSWLLARGPVPEQMLAQARVPLGDEMT
  VCQCEALASPGPPAPHMEAEGPVDVRHVQWLMGKLRFRWRQLVSSQQEQCALLSKIHLYTRGCHSDQSLS
  HLSVTEAEMLRDPEGGQQLLRTLERENQRLEAVLAWRRSELVFWRWMDTVLGTCAPEVPAAASQPTFLPW
  VPERGGGELDLVVRELQALEEELREAAERRRAAWEAKAGGCGRGPEWSAARRASREAVEKELGALQQCWE
  RDGGPAQPHGPHRLVRREDGAAGDRDLRAAVVIRTLRSQEACLEAVLRRLQGQCRQELARLVGARPGLIW
  IPPPGR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001134875
• ORF Size: 1278 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134875.1, NP_001128347.1
• RefSeq ORF: 1281 bp
• Locus ID: 283643
• UniProt ID: Q86SX3
• Cytogenetics: 14q32.33
• MW: 46.9 kDa
• Gene Summary: May play a role in counteracting perturbation of actin filaments, such as after treatment with the actin depolymerizing microbial metabolite Chivosazole F.[UniProtKB/Swiss-Prot Function]