Sialoadhesin (SIGLEC1) (NM_023068) Human Tagged ORF Clone

CAT#: RC224969

SIGLEC1 (Myc-DDK-tagged)-Human sialic acid binding Ig-like lectin 1, sialoadhesin (SIGLEC1)

Product Images

Circular map for RC224969

• 

  Western blot validation of overexpression lysate (Cat# LY411501) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224969 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SIGLEC1 protein (Cat# TP324969). The protein was produced from HEK293T cells transfected with SIGLEC1 cDNA clone (Cat# RC224969) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sialoadhesin
• Synonyms: CD169; SIGLEC-1; SN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224969 representing NM_023068.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGCTTCTTGCCCAAGCTTCTCCTCCTGGCCTCATTCTTCCCAGCAGGCCAGGCCTCATGGGGCGTC
  TCCAGTCCCCAGGACGTGCAGGGTGTGAAGGGGTCTTGCCTGCTTATCCCCTGCATCTTCAGCTTCCCT
  GCCGACGTGGAGGTGCCCGACGGCATCACGGCCATCTGGTACTACGACTACTCGGGCCAGCGGCAGGTG
  GTGAGCCACTCGGCGGACCCCAAGCTGGTGGAGGCCCGCTTCCGCGGCCGCACCGAGTTCATGGGGAAC
  CCCGAGCACAGGGTGTGCAACCTGCTGCTGAAGGACCTGCAGCCCGAGGACTCTGGTTCCTACAACTTC
  CGCTTCGAGATCAGTGAGGTCAACCGCTGGTCAGATGTGAAAGGCACCTTGGTCACAGTAACAGAGGAG
  CCCAGGGTGCCCACCATTGCCTCCCCGGTGGAGCTTCTCGAGGGCACAGAGGTGGACTTCAACTGCTCC
  ACTCCCTACGTATGCCTGCAGGAGCAGGTCAGACTGCAGTGGCAAGGCCAGGACCCTGCTCGCTCTGTC
  ACCTTCAACAGCCAGAAGTTTGAGCCCACCGGCGTCGGCCACCTGGAGACCCTCCACATGGCCATGTCC
  TGGCAGGACCACGGCCGGATCCTGCGCTGCCAGCTCTCCATGGCCAATCACAGGGCTCAGAGCGAGATT
  CACCTCCAAGTGAAGTATGCCCCCAGGGGTGTGAAGATCCTCCTCAGCCCCTCGGGGAGGAACATCCTT
  CCAGGTGAGCTGGTCACACTCACCTGCCAGGTGAACAGCAGCTACCCTGCAGTCAGTTCCATTAAGTGG
  CTCAAGGATGGGGTACGCCTCCAAACCAAGACTGGTGTGCTGCACCTGCCCCAGGCAGCCTGGAGCGAT
  GCTGGCGTCTACACCTGCCAAGCTGAGAACGGCGTGGGCTCTTTGGTCTCACCCCCCATCAGCCTCCAC
  ATCTTCATGGCTGAGGTCCAGGTGAGCCCAGCAGGTCCCATCCTGGAGAACCAGACAGTGACACTAGTC
  TGCAACACACCCAACGAGGCACCCAGTGATCTCCGCTACAGCTGGTACAAGAACCATGTCCTGCTGGAG
  GATGCCCACTCCCATACCCTCCGGCTGCACTTGGCCACTAGGGCTGATACTGGCTTCTACTTCTGTGAG
  GTGCAGAACGTCCATGGCAGCGAGCGCTCGGGCCCTGTCAGCGTGGTAGTCAACCACCCGCCTCTCACC
  CCAGTCCTGACAGCCTTCCTGGAGACCCAGGCGGGACTTGTGGGCATCCTTCACTGCTCTGTGGTCAGT
  GAGCCCCTGGCCACACTGGTGCTGTCACATGGGGGTCATATCCTGGCCTCCACCTCCGGGGACAGTGAT
  CACAGCCCACGCTTCAGTGGTACCTCTGGTCCCAACTCCCTGCGCCTGGAGATCCGAGACCTGGAGGAA
  ACTGACAGTGGGGAGTACAAGTGCTCAGCCACCAACTCCCTTGGAAATGCAACCTCTACCCTGGACTTC
  CATGCCAATGCCGCCCGTCTCCTCATCAGCCCGGCAGCCGAGGTGGTGGAAGGACAGGCAGTGACACTG
  AGCTGCAGAAGCGGCCTAAGCCCCACACCTGATGCCCGCTTCTCCTGGTACCTGAATGGAGCCCTGCTT
  CACGAGGGTCCCGGCAGCAGCCTCCTGCTCCCCGCGGCCTCCAGCACTGACGCCGGCTCATACCACTGC
  CGGGCCCGGGACGGCCACAGTGCCAGTGGCCCCTCTTCGCCAGCTGTTCTCACTGTGCTCTACCCCCCT
  CGACAACCAACATTCACCACCAGGCTGGACCTTGATGCCGCTGGGGCCGGGGCTGGACGGCGAGGCCTC
  CTTTTGTGCCGTGTGGACAGCGACCCCCCCGCCAGGCTGCAGCTGCTCCACAAGGACCGTGTTGTGGCC
  ACTTCCCTGCCATCAGGGGGTGGCTGCAGCACCTGTGGGGGCTGTTCCCCACGCATGAAGGTCACCAAA
  GCCCCCAACTTGCTGCGTGTGGAGATTCACAACCCTTTGCTGGAAGAGGAGGGCTTGTACCTCTGTGAG
  GCCAGCAATGCCCTGGGCAACGCCTCCACCTCAGCCACCTTCAATGGCCAGGCCACTGTCCTGGCCATT
  GCACCATCACACACACTTCAGGAGGGCACAGAAGCCAACTTGACTTGCAACGTGAGCCGGGAAGCTGCT
  GGCAGCCCTGCTAACTTCTCCTGGTTCCGAAATGGGGTGCTGTGGGCCCAGGGTCCCCTGGAGACCGTG
  ACACTGCTGCCCGTGGCCAGAACTGATGCTGCCCTTTACGCCTGCCGCATCCTGACTGAGGCTGGTGCC
  CAGCTCTCCACTCCCGTGCTCCTGAGTGTCCTCTATCCCCCGGACCGTCCAAAGCTGTCAGCCCTCCTA
  GACATGGGCCAGGGCCACATGGCTCTGTTCATCTGCACTGTGGACAGCCGCCCCCTGGCCTTGCTGGCC
  TTGTTCCATGGGGAGCACCTCCTGGCCACCAGCCTGGGTCCCCAGGTCCCATCCCATGGTCGGTTCCAG
  GCTAAGGCTGAGGCCAACTCCCTGAAGTTAGAGGTCCGAGAACTGGGCCTTGGGGACTCTGGCAGCTAC
  CGCTGTGAGGCCACAAATGTTCTTGGATCATCCAACACCTCACTCTTCTTCCAGGTCCGAGGAGCCTGG
  GTCCAGGTGTCACCATCACCTGAGCTCCAAGAGGGCCAGGCTGTGGTCCTGAGCTGCCAGGTACCCACA
  GGAGTCCCAGAGGGGACCTCATATCGTTGGTATCGGGATGGCCAGCCCCTCCAGGAGTCGACCTCGGCC
  ACGCTCCGCTTTGCAGCCATAACTTTGACACAAGCTGGGGCCTATCATTGCCAAGCCCAGGCCCCAGGC
  TCAGCCACCACGAGCCTAGCTGTACCCATCAGCCTCCACGTGTCCTATGCCCCACGCCACGTCACACTC
  ACTACCCTGATGGACACAGGCCCTGGACGACTGGGCCTCCTCCTGTGCCGTGTGGACAGTGACCCTCCG
  GCCCAGCTGCGGCTGCTCCACGGGGATCGCCTTGTGGCCTCCACCCTACAAGGTGTGGGGGGACCCGAA
  GGCAGCTCTCCCAGGCTGCATGTGGCTGTGGCCCCCAACACACTGCGTCTGGAGATCCACGGGGCTATG
  CTGGAGGATGAGGGTGTCTATATCTGTGAGGCCTCCAACACCCTGGGCCAGGCCTCGGCCTCAGCTGAC
  TTCGACGCTCAAGCTGTGAATGTGCAGGTGTGGCCCGGGGCTACCGTGCGGGAGGGGCAGCTGGTGAAC
  CTGACCTGCCTTGTGTGGACCACTCACCCGGCCCAGCTCACCTACACATGGTACCAGGATGGGCAGCAG
  CGCCTGGATGCCCACTCCATCCCCCTGCCCAACGTCACAGTCAGGGATGCCACCTCCTACCGCTGCGGT
  GTGGGCCCCCCTGGTCGGGCACCCCGCCTCTCCAGACCTATCACCTTGGACGTCCTCTACGCGCCCCGC
  AACCTGCGCCTGACCTACCTCCTGGAGAGCCATGGCGGGCAGCTGGCCCTGGTACTGTGCACTGTGGAC
  AGCCGCCCGCCCGCCCAGCTGGCCCTCAGCCACGCCGGTCGCCTCTTGGCCTCCTCGACAGCAGCCTCT
  GTCCCCAACACCCTGCGCCTGGAGCTGCGAGGGCCACAGCCCAGGGATGAGGGTTTCTACAGCTGCTCT
  GCCCGCAGCCCTCTGGGCCAGGCCAACACGTCCCTGGAGCTGCGGCTGGAGGGTGTGCGGGTGATCCTG
  GCTCCGGAGGCTGCCGTGCCTGAAGGTGCCCCCATCACAGTGACCTGTGCGGACCCTGCTGCCCACGCA
  CCCACACTCTATACTTGGTACCACAACGGTCGTTGGCTGCAGGAGGGTCCAGCTGCCTCACTCTCATTC
  CTGGTGGCCACGCGGGCTCATGCAGGCGCCTACTCTTGCCAGGCCCAGGATGCCCAGGGCACCCGCAGC
  TCCCGTCCTGCTGCCCTGCAAGTCCTCTATGCCCCTCAGGATGCTGTCCTGTCCTCCTTCCGGGACTCC
  AGGGCCAGATCCATGGCTGTGATACAGTGCACTGTGGACAGTGAGCCACCTGCTGAGCTGGCCCTATCT
  CATGATGGCAAGGTGCTGGCCACGAGCAGCGGGGTCCACAGCTTGGCATCAGGGACAGGCCATGTCCAG
  GTGGCCCGAAACGCCCTACGGCTGCAGGTGCAAGATGTGCCTGCAGGTGATGACACCTATGTTTGCACA
  GCCCAAAACTTGCTGGGCTCAATCAGCACCATCGGGCGGTTGCAGGTAGAAGGTGCACGCGTGGTGGCA
  GAGCCTGGCCTGGACGTGCCTGAGGGCGCTGCCCTGAACCTCAGCTGCCGCCTCCTGGGTGGCCCTGGG
  CCTGTGGGCAACTCCACCTTTGCATGGTTCTGGAATGACCGGCGGCTGCACGCGGAGCCTGTGCCCACT
  CTCGCCTTCACCCACGTGGCTCGTGCTCAAGCTGGGATGTACCACTGCCTGGCTGAGCTCCCCACTGGG
  GCTGCTGCCTCTGCTCCAGTCATGCTCCGTGTGCTCTACCCTCCCAAGACGCCCACCATGATGGTCTTC
  GTGGAGCCTGAGGGTGGCCTCCGGGGCATCCTGGATTGCCGAGTGGACAGCGAGCCGCTCGCCAGCCTG
  ACTCTCCACCTTGGCAGTCGACTGGTGGCCTCCAGTCAGCCCCAGGGTGCTCCTGCAGAGCCACACATC
  CATGTCCTGGCTTCCCCCAATGCCCTGAGGGTGGACATCGAGGCGCTGAGGCCCAGCGACCAAGGGGAA
  TACATCTGTTCTGCCTCAAATGTCCTGGGCTCTGCCTCTACCTCCACCTACTTTGGGGTCAGAGCCCTG
  CACCGCCTGCATCAGTTCCAGCAGCTGCTCTGGGTCCTGGGACTGCTGGTGGGCCTCCTGCTCCTGCTG
  TTGGGCCTGGGGGCCTGCTACACCTGGAGAAGGAGGCGTGTTTGTAAGCAGAGCATGGGCGAGAATTCG
  GTGGAGATGGCTTTTCAGAAAGAGACCACGCAGCTCATTGATCCTGATGCAGCCACATGTGAGACCTCA
  ACCTGTGCCCCACCCCTGGGC
  CTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGAC
  GATAAGGTTTAAACGGCCGGCCGCGGTCATAGCTGT
  
  Protein Sequence

  >Peptide sequence encoded by RC224969
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MGFLPKLLLLASFFPAGQASWGVSSPQDVQGVKGSCLLIPCIFSFPADVEVPDGITAIWYYDYSGQRQV
  VSHSADPKLVEARFRGRTEFMGNPEHRVCNLLLKDLQPEDSGSYNFRFEISEVNRWSDVKGTLVTVTEE
  PRVPTIASPVELLEGTEVDFNCSTPYVCLQEQVRLQWQGQDPARSVTFNSQKFEPTGVGHLETLHMAMS
  WQDHGRILRCQLSMANHRAQSEIHLQVKYAPRGVKILLSPSGRNILPGELVTLTCQVNSSYPAVSSIKW
  LKDGVRLQTKTGVLHLPQAAWSDAGVYTCQAENGVGSLVSPPISLHIFMAEVQVSPAGPILENQTVTLV
  CNTPNEAPSDLRYSWYKNHVLLEDAHSHTLRLHLATRADTGFYFCEVQNVHGSERSGPVSVVVNHPPLT
  PVLTAFLETQAGLVGILHCSVVSEPLATLVLSHGGHILASTSGDSDHSPRFSGTSGPNSLRLEIRDLEE
  TDSGEYKCSATNSLGNATSTLDFHANAARLLISPAAEVVEGQAVTLSCRSGLSPTPDARFSWYLNGALL
  HEGPGSSLLLPAASSTDAGSYHCRARDGHSASGPSSPAVLTVLYPPRQPTFTTRLDLDAAGAGAGRRGL
  LLCRVDSDPPARLQLLHKDRVVATSLPSGGGCSTCGGCSPRMKVTKAPNLLRVEIHNPLLEEEGLYLCE
  ASNALGNASTSATFNGQATVLAIAPSHTLQEGTEANLTCNVSREAAGSPANFSWFRNGVLWAQGPLETV
  TLLPVARTDAALYACRILTEAGAQLSTPVLLSVLYPPDRPKLSALLDMGQGHMALFICTVDSRPLALLA
  LFHGEHLLATSLGPQVPSHGRFQAKAEANSLKLEVRELGLGDSGSYRCEATNVLGSSNTSLFFQVRGAW
  VQVSPSPELQEGQAVVLSCQVPTGVPEGTSYRWYRDGQPLQESTSATLRFAAITLTQAGAYHCQAQAPG
  SATTSLAVPISLHVSYAPRHVTLTTLMDTGPGRLGLLLCRVDSDPPAQLRLLHGDRLVASTLQGVGGPE
  GSSPRLHVAVAPNTLRLEIHGAMLEDEGVYICEASNTLGQASASADFDAQAVNVQVWPGATVREGQLVN
  LTCLVWTTHPAQLTYTWYQDGQQRLDAHSIPLPNVTVRDATSYRCGVGPPGRAPRLSRPITLDVLYAPR
  NLRLTYLLESHGGQLALVLCTVDSRPPAQLALSHAGRLLASSTAASVPNTLRLELRGPQPRDEGFYSCS
  ARSPLGQANTSLELRLEGVRVILAPEAAVPEGAPITVTCADPAAHAPTLYTWYHNGRWLQEGPAASLSF
  LVATRAHAGAYSCQAQDAQGTRSSRPAALQVLYAPQDAVLSSFRDSRARSMAVIQCTVDSEPPAELALS
  HDGKVLATSSGVHSLASGTGHVQVARNALRLQVQDVPAGDDTYVCTAQNLLGSISTIGRLQVEGARVVA
  EPGLDVPEGAALNLSCRLLGGPGPVGNSTFAWFWNDRRLHAEPVPTLAFTHVARAQAGMYHCLAELPTG
  AAASAPVMLRVLYPPKTPTMMVFVEPEGGLRGILDCRVDSEPLASLTLHLGSRLVASSQPQGAPAEPHI
  HVLASPNALRVDIEALRPSDQGEYICSASNVLGSASTSTYFGVRALHRLHQFQQLLWVLGLLVGLLLLL
  LGLGACYTWRRRRVCKQSMGENSVEMAFQKETTQLIDPDAATCETSTCAPPLG
  LEQKLISEEDLAANDILDYKDDDDKV
  
  Recombinant protein using RC224969 also available, TP324969M
• Restriction Sites: SgfI-XhoI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023068
• ORF Size: 5127 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 5130 bp
• RefSeq ORF: 5130 bp
• Locus ID: 6614
• UniProt ID: Q9BZZ2
• Cytogenetics: 20p13
• Protein Families: Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• MW: 182.7 kDa
• Gene Summary: This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008]