CEACAM16 (NM_001039213) Human Tagged ORF Clone

CAT#: RC224965

CEACAM16 (Myc-DDK-tagged)-Human carcinoembryonic antigen-related cell adhesion molecule 16 (CEACAM16)

Product Images

Circular map for RC224965

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  Western blot validation of overexpression lysate (Cat# LY422030) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224965 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CEACAM16 protein (Cat# TP324965). The protein was produced from HEK293T cells transfected with CEACAM16 cDNA clone (Cat# RC224965) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CEACAM16
• Synonyms: CEAL2; DFNA4B; DFNB113
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224965 representing NM_001039213
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGATTTACTGAGCATTTACTCTGCGCCAGTCGTGGTAAGTACTGTACTTCACATGTTACAAATAA
  GGAAACTGAGGCTTGGCAGAGTCCATAAATTGCCCAGGGTCCTGGAGGTAGGAAACAGTGGAACTATGTC
  CAGAGCCTATACTTTTTTTTTTTTGAGATGGAGTTTCATGGCGCTGACTGGGTACAGCTGGCTGCTCCTC
  AGTGCCACATTCCTGAATGTGGGGGCCGAGATCTCTATCACCCTGGAGCCTGCCCAGCCGAGCGAAGGGG
  ACAACGTCACGCTGGTCGTCCATGGGCTTTCGGGGGAACTGCTCGCCTACAGCTGGTATGCGGGGCCCAC
  ACTCAGCGTGTCATACCTGGTGGCCAGCTACATCGTGAGCACAGGCGATGAGACTCCTGGCCCGGCCCAC
  ACGGGGCGGGAGGCTGTGCGCCCCGATGGCAGCCTGGACATCCAGGGCATCCTGCCCCGGCACTCAGGCA
  CCTACATCCTGCAGACCTTCAACAGGCAGTTGCAGACCGAGGTGGGCTACGGACACGTGCAGGTCCATGA
  GATCCTGGCCCAGCCCACAGTCTTGGCCAACAGCACAGCGCTGGTGGAACGTCGAGACACCCTGCGCCTT
  ATGTGCAGCAGCCCCAGCCCCACCGCCGAGGTCCGCTGGTTCTTCAACGGTGGGGCCCTGCCCGTCGCTC
  TCCGCCTGGGCCTGTCCCCTGACGGCCGGGTGCTGGCCAGGCATGGCATCCGCCGGGAGGAGGCCGGCGC
  CTATCAGTGTGAGGTGTGGAACCCGGTCAGTGTCAGCCGCAGCGAGCCCATCAACCTGACCGTGTACTTT
  GGCCCAGAGCGTGTGGCCATCCTCCAGGATTCCACCACCCGCACAGGCTGCACCATCAAAGTTGACTTCA
  ACACGTCCCTCACCCTGTGGTGCGTGTCCAGGTCCTGCCCAGAGCCCGAGTATGTGTGGACCTTCAACGG
  GCAGGCCCTAAAGAACGGCCAAGACCACCTCAACATCAGCAGCATGACAGCCGCCCAGGAGGGGACGTAC
  ACATGTATTGCGAAGAACACCAAGACCCTGCTATCTGGATCTGCCTCAGTCGTGGTCAAGCTCTCTGCTG
  CAGCAGTTGCCACGATGATCGTGCCCGTGCCCACCAAGCCAACGGAGGGCCAGGACGTAACACTGACCGT
  GCAGGGCTACCCCAAGGACCTGCTGGTCTACGCCTGGTACCGCGGGCCTGCCTCCGAGCCCAACCGGCTG
  CTCAGCCAGCTGCCGTCAGGAACCTGGATTGCAGGCCCCGCGCACACAGGCCGGGAGGTGGGCTTCCCCA
  ACTGCTCGCTGTTGGTGCAGAAGCTGAACCTCACAGACACTGGCCGCTACACACTCAAGACCGTCACAGT
  GCAGGGCAAGACTGAGACACTGGAAGTGGAGCTGCAGGTGGCCCCCCTGGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224965 representing NM_001039213
  Red=Cloning site Green=Tags(s)
  
  MSDLLSIYSAPVVVSTVLHMLQIRKLRLGRVHKLPRVLEVGNSGTMSRAYTFFFLRWSFMALTGYSWLLL
  SATFLNVGAEISITLEPAQPSEGDNVTLVVHGLSGELLAYSWYAGPTLSVSYLVASYIVSTGDETPGPAH
  TGREAVRPDGSLDIQGILPRHSGTYILQTFNRQLQTEVGYGHVQVHEILAQPTVLANSTALVERRDTLRL
  MCSSPSPTAEVRWFFNGGALPVALRLGLSPDGRVLARHGIRREEAGAYQCEVWNPVSVSRSEPINLTVYF
  GPERVAILQDSTTRTGCTIKVDFNTSLTLWCVSRSCPEPEYVWTFNGQALKNGQDHLNISSMTAAQEGTY
  TCIAKNTKTLLSGSASVVVKLSAAAVATMIVPVPTKPTEGQDVTLTVQGYPKDLLVYAWYRGPASEPNRL
  LSQLPSGTWIAGPAHTGREVGFPNCSLLVQKLNLTDTGRYTLKTVTVQGKTETLEVELQVAPLG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039213
• ORF Size: 1452 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039213.1, NP_001034302.1
• RefSeq Size: 1797 bp
• RefSeq ORF: 1278 bp
• Locus ID: 388551
• UniProt ID: Q2WEN9
• Cytogenetics: 19q13.31-q13.32
• Protein Families: Transmembrane
• MW: 52.68 kDa
• Gene Summary: The protein encoded by this gene is a secreted glycoprotein that in mouse interacts with tectorial membrane proteins in the inner ear. The encoded adhesion protein is found in cochlear outer hair cells and appears to be important for proper hearing over an extended frequency range. Defects in this gene likely are a cause of non-syndromic autosomal dominant hearing loss. [provided by RefSeq, May 2012]