TAF8 (NM_138572) Human Tagged ORF Clone

CAT#: RC224879

TAF8 (Myc-DDK-tagged)-Human TAF8 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 43kDa (TAF8)

Product Images

Circular map for RC224879

• 

  Western blot validation of overexpression lysate (Cat# LY408568) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224879 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TAF8
• Synonyms: II; TAF; TAF(II)43; TAFII-43; TAFII43; TBN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224879 representing NM_138572
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACGCGGCGGCCACAGCTGGGGCCGGTGGCTCCGGAACGAGATCGGGAAGTAAACAGTCCACTA
  ACCCTGCCGATAACTATCATCTGGCCCGGAGGAGAACCCTGCAGGTGGTTGTGAGCTCCTTGCTGACAGA
  GGCAGGGTTTGAGAGTGCCGAGAAAGCATCCGTGGAAACGCTGACAGAGATGCTGCAGAGCTACATTTCA
  GAAATTGGGAGAAGTGCCAAGTCTTACTGTGAGCACACAGCCAGGACCCAGCCCACACTGTCCGATATCG
  TGGTCACACTTGTTGAGATGGGTTTCAATGTGGACACTCTCCCTGCTTATGCAAAACGGTCTCAGAGGAT
  GGTCATCACTGCTCCTCCGGTGACCAATCAGCCAGTGACCCCCAAGGCCCTCACTGCAGGGCAGAACCGA
  CCCCACCCGCCGCACATCCCCAGCCATTTTCCTGAGTTCCCTGATCCCCACACCTACATCAAAACTCCGA
  CGTACCGTGAGCCCGTGTCAGACTACCAGGTCCTGCGGGAGAAGGCTGCATCCCAGAGGCGCGATGTGGA
  GCGGGCACTTACCCGTTTCATGGCCAAGACAGGCGAGACTCAGAGTCTTTTCAAAGATGACGTCAGCACA
  TTTCCATTGATTGCTGCCAGACCTTTCACCATCCCCTACCTGACAGCTCTTCTTCCGTCTGAACTGGAGA
  TGCAACAAATGGAAGAGACAGATTCCTCGGAGCAGGATGAACAGACAGACACAGAGAACCTTGCTCTTCA
  TATCAGCATGGAGGATTCTGGAGCCGAGAAGGAGAACACCTCTGTCCTGCAGCAGAACCCCTCCTTGTCG
  GGTAGCCGGAATGGGGAGGAGAACATCATCGATAACCCTTATCTGCGGCCGGTGAAGAAGCCCAAGATCC
  GCAGGAAGAAGTCCCTCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224879 representing NM_138572
  Red=Cloning site Green=Tags(s)
  
  MADAAATAGAGGSGTRSGSKQSTNPADNYHLARRRTLQVVVSSLLTEAGFESAEKASVETLTEMLQSYIS
  EIGRSAKSYCEHTARTQPTLSDIVVTLVEMGFNVDTLPAYAKRSQRMVITAPPVTNQPVTPKALTAGQNR
  PHPPHIPSHFPEFPDPHTYIKTPTYREPVSDYQVLREKAASQRRDVERALTRFMAKTGETQSLFKDDVST
  FPLIAARPFTIPYLTALLPSELEMQQMEETDSSEQDEQTDTENLALHISMEDSGAEKENTSVLQQNPSLS
  GSRNGEENIIDNPYLRPVKKPKIRRKKSLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138572
• ORF Size: 930 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138572.3
• RefSeq Size: 4323 bp
• RefSeq ORF: 933 bp
• Locus ID: 129685
• UniProt ID: Q7Z7C8
• Cytogenetics: 6p21.1
• MW: 34.1 kDa
• Gene Summary: This gene encodes one of several TATA-binding protein (TBP)-associated factors (TAFs), which are integral subunits of the general transcription factor complex TFIID. TFIID recognizes the core promoter of many genes and nucleates the assembly of a transcription preinitiation complex containing RNA polymerase II and other initiation factors. The protein encoded by this gene contains an H4-like histone fold domain, and interacts with several subunits of TFIID including TBP and the histone-fold protein TAF10. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]