SORBS1 (NM_001034955) Human Tagged ORF Clone

CAT#: RC224759

SORBS1 (Myc-DDK-tagged)-Human sorbin and SH3 domain containing 1 (SORBS1), transcript variant 4

Product Images

Circular map for RC224759

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  Western blot validation of overexpression lysate (Cat# LY422115) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224759 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SORBS1 protein (Cat# TP324759). The protein was produced from HEK293T cells transfected with SORBS1 cDNA clone (Cat# RC224759) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SORBS1
• Synonyms: CAP; FLAF2; R85FL; SH3D5; SH3P12; SORB1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224759 representing NM_001034955
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCTGAATGTGATGGTGGTTCCAAAGCTGTGATGAATGGCTTGGCACCTGGCAGCAATGGGCAAG
  ACAAAGCAACTGCCGACCCTTTACGCGCACGCTCTATTTCTGCTGTTAAAATCATTCCTGTGAAGACAGT
  GAAAAACGCCTCAGGCCTAGTTCTCCCTACAGACATGGATCCTACAAAAATCTGCACTGGGAAGGGAGCG
  GTGACTCTCCGGGCCTCGTCTTCCTACAGGGAAACCCCAAGCAGTAGCCCTGCGAGCCCTCAGGAAACCC
  GGCAACACGAAAGCAAACCAGGTCTGGAGCCAGAGCCTTCTTCAGCAGATGAGTGGAGGCTTTCTTCCAG
  TGCTGATGCCAATGGAAATGCCCAGCCCTCTTCACTCGCTGCCAAGGGCTACAGAAGTGTGCATCCCAAC
  CTTCCTTCTGACAAGTCCCAGGATGCCACTTCCTCCAGTGCAGCCCAGCCGGAGGTAATAGTTGTCCCTC
  TCTACCTGGTTAATACTGACAGAGGGCAAGAAGGCACTGCCAGACCTCCAACACCTCTGGGGCCTCTTGG
  CTGCGTCCCCACAATCCCAGCGACTGCCTCTGCCGCCTCACCTCTGACCTTCCCGACTCTAGATGATTTC
  ATTCCCCCTCATCTGCAGAGGTGGCCCCACCACAGCCAGCCAGCCCGCGCCTCTGGCTCCTTTGCCCCCA
  TTAGCCAGACGCCACCATCCTTCTCACCACCACCTCCGCTGGTCCCTCCTGCCCCGGAGGACCTCCGCAG
  AGTCTCGGAGCCTGACCTCACGGGAGCTGTTTCGAGTACCGATTCCAGTCCTCTACTAAATGAAGTTTCT
  TCTTCCCTTATTGGAACTGATTCCCAAGCCTTTCCATCAGTTAGCAAGCCTTCATCCGCCTATCCCTCCA
  CAACGATTGTCAATCCTACTATTGTGCTCTTGCAACACAATCGAGAACAGCAAAAACGACTCAGTAGCCT
  TTCAGATCCTGTCTCAGAAAGAAGAGTGGGAGAGCAGGACTCAGCACCAACCCAGGAAAAACCCACCTCA
  CCTGGCAAGGCTATTGAAAAAAGAGCAAAGGATGACAGTAGGCGGGTGGTGAAGAGCACTCAGGACTTAA
  GCGATGTTTCCATGGATGAAGTGGGCATCCCACTCCGGAACACTGAGAGATCAAAAGACTGGTACAAGAC
  TATGTTTAAACAGATCCACAAACTGAACAGAGACACTCCTGAAGAAAACCCTTATTTCCCTACGTACAAA
  TTCCCTGAACTTCCTGAAATCCAGCAAACTTCCGAAGAGACAAAGTCTTGCTCTGTCATGTCACCCAGGC
  TGGAGTGCAGTGGTACAGTCATAGCTCACTGCAGCCTCAAACTCCTAGACTCAAGCAATCCTCCCACCTC
  AGCCTCCCAAGTAGCTGGGACTGCAGATGATGATTCAGATCTGTACTCTCCCAGATACTCATTTTCTGAA
  GACACAAAATCTCCCCTTTCTGTGCCTCGCTCAAAAAGTGAGATGAGCTACATTGATGGTGAGAAGGTAG
  TCAAGAGGTCGGCCACACTACCCCTCCCAGCCCGCTCTTCCTCACTGAAGTCAAGCTCAGAAAGAAATGA
  CTGGGAACCCCCAGATAAGAAAGTAGACACAAGAAAATATCGTGCAGAGCCCAAGAGCATTTACGAATAT
  CAGCCTGGCAAGTCTTCCGTTCTGACCAACGAAAAGATGAGTCGGGATATAAGCCCAGAAGAGATAGATT
  TAAAGAATGAACCTTGGTATAAATTCTTTTCGGAATTGGAGTTTGGGAAACCGCCTCCCAAAAAGATATG
  GGATTATACTCCTGGAGACTGCTCTATCCTTCCTAGAGAGGATAGAAAGACTAATCTAGACAAAGATCTC
  AGCCTCTGCCAGACAGAGTTAGAGGCAGATTTAGAAAAAATGGAGACGCTTAATAAAGCACCCAGTGCAA
  ACGTGCCACAGAGCTCAGCCATCAGCCCTACTCCGGAAATTTCTTCAGAGACTCCTGGATATATATATTC
  TTCCAACTTCCATGCAGTGAAGAGGGAATCAGACGGGGCTCCTGGGGATCTCACTAGCTTGGAGAATGAG
  AGACAAATTTATAAAAGTGTCTTGGAAGGTGGTGACATCCCTCTTCAGGGCCTGAGTGGGCTCAAGCGAC
  CATCCAGCTCTGCTTCCACTAAAGATTCAGAATCGCCAAGACATTTTATACCAGCTGATTACTTGGAATC
  CACGGAAGAATTTATTCGAAGACGTCATGATGATAAAGAGAAACTTTTAGCGGACCAGAGACGACTTAAA
  CGCGAGCAAGAAGAGGCTGATATTGCAGCTCGACGCCACACAGGCGTCATTCCGACGCACCATCAGTTTA
  TCACTAATGAGCGCTTTGGGGACCTCCTCAATATAGACGATACTGCAAAAAGGAAATCTGGGTCAGAGAT
  GAGACCTGCCAGAGCCAAATTTGACTTTAAAGCTCAGACACTAAAGGAGCTTCCTCTGCAGAAGGGAGAT
  ATTGTTTACATTTATAAGCAAATTGATCAGAACTGGTATGAAGGAGAACACCACGGCCGGGTGGGAATCT
  TCCCACGCACCTACATCGAGCTTCTTCCTCCTGCTGAGAAGGCACAGCCCAAAAAGTTGACACCAGTGCA
  GGTTTTGGAATATGGAGAAGCTATTGCTAAGTTTAACTTTAATGGTGATACACAAGTAGAAATGTCCTTC
  AGAAAGGGTGAGAGGATCACACTGCTCCGGCAGGTAGATGAGAACTGGTACGAAGGGAGGATCCCGGGGA
  CATCCCGACAAGGCATCTTCCCCATCACCTACGTGGATGTGATCAAGCGACCACTGGTGAAAAACCCTGT
  GGATTACATGGACCTGCCTTTCTCCTCCTCCCCAAGTCGCAGTGCCACTGCAAGCCCACAGCCATGGCGC
  GAAGAGAGTGGACAATATGAGAGGAAAGCAGAGAGGGGGGCAGGCGAAAGAGGCCCTGGTGGACCCAAGA
  TCTCTAAGAAGAGCTGCTTGAAGCCTTCAGACGTGGTCAGGTGCCTGAGTACTGAACAGAGACTCTCAGA
  TCTCAACACCCCTGAGGAGAGCCGGCCCGGCAAGCCCCTGGGTAGCGCTTTTCCAGGAAGTGAGGCTGAG
  CAGACAGAGCGGCATAGAGGTGGCGAGCAGGCGGGGAGGAAAGCTGCTCGGAGAGGTGGGAGCCAGCAAC
  CTCAAGCCCAGCAGCGAAGAGTCACCCCCGACAGGAGTCAAACCTCACAAGATTTATTTAGCTATCAAGC
  ATTATATAGCTATATACCACAGAATGATGATGAGTTGGAACTCCGCGATGGAGATATCGTTGATGTCATG
  GAAAAATGTGACGATGGATGGTTTGTTGGTACTTCAAGAAGGACAAAGCAGTTTGGTACTTTTCCAGGCA
  ACTATGTAAAACCTTTGTATCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224759 representing NM_001034955
  Red=Cloning site Green=Tags(s)
  
  MSSECDGGSKAVMNGLAPGSNGQDKATADPLRARSISAVKIIPVKTVKNASGLVLPTDMDPTKICTGKGA
  VTLRASSSYRETPSSSPASPQETRQHESKPGLEPEPSSADEWRLSSSADANGNAQPSSLAAKGYRSVHPN
  LPSDKSQDATSSSAAQPEVIVVPLYLVNTDRGQEGTARPPTPLGPLGCVPTIPATASAASPLTFPTLDDF
  IPPHLQRWPHHSQPARASGSFAPISQTPPSFSPPPPLVPPAPEDLRRVSEPDLTGAVSSTDSSPLLNEVS
  SSLIGTDSQAFPSVSKPSSAYPSTTIVNPTIVLLQHNREQQKRLSSLSDPVSERRVGEQDSAPTQEKPTS
  PGKAIEKRAKDDSRRVVKSTQDLSDVSMDEVGIPLRNTERSKDWYKTMFKQIHKLNRDTPEENPYFPTYK
  FPELPEIQQTSEETKSCSVMSPRLECSGTVIAHCSLKLLDSSNPPTSASQVAGTADDDSDLYSPRYSFSE
  DTKSPLSVPRSKSEMSYIDGEKVVKRSATLPLPARSSSLKSSSERNDWEPPDKKVDTRKYRAEPKSIYEY
  QPGKSSVLTNEKMSRDISPEEIDLKNEPWYKFFSELEFGKPPPKKIWDYTPGDCSILPREDRKTNLDKDL
  SLCQTELEADLEKMETLNKAPSANVPQSSAISPTPEISSETPGYIYSSNFHAVKRESDGAPGDLTSLENE
  RQIYKSVLEGGDIPLQGLSGLKRPSSSASTKDSESPRHFIPADYLESTEEFIRRRHDDKEKLLADQRRLK
  REQEEADIAARRHTGVIPTHHQFITNERFGDLLNIDDTAKRKSGSEMRPARAKFDFKAQTLKELPLQKGD
  IVYIYKQIDQNWYEGEHHGRVGIFPRTYIELLPPAEKAQPKKLTPVQVLEYGEAIAKFNFNGDTQVEMSF
  RKGERITLLRQVDENWYEGRIPGTSRQGIFPITYVDVIKRPLVKNPVDYMDLPFSSSPSRSATASPQPWR
  EESGQYERKAERGAGERGPGGPKISKKSCLKPSDVVRCLSTEQRLSDLNTPEESRPGKPLGSAFPGSEAE
  QTERHRGGEQAGRKAARRGGSQQPQAQQRRVTPDRSQTSQDLFSYQALYSYIPQNDDELELRDGDIVDVM
  EKCDDGWFVGTSRRTKQFGTFPGNYVKPLYL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034955
• ORF Size: 3453 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034955.1, NP_001030127.1
• RefSeq Size: 6803 bp
• RefSeq ORF: 3456 bp
• Locus ID: 10580
• UniProt ID: Q9BX66
• Cytogenetics: 10q24.1
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Insulin signaling pathway, PPAR signaling pathway
• MW: 127.1 kDa
• Gene Summary: This gene encodes a CBL-associated protein which functions in the signaling and stimulation of insulin. Mutations in this gene may be associated with human disorders of insulin resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]