MPEG1 (NM_001039396) Human Tagged ORF Clone

CAT#: RC224739

MPEG1 (Myc-DDK-tagged)-Human macrophage expressed 1 (MPEG1)

Product Images

Circular map for RC224739

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  Western blot validation of overexpression lysate (Cat# LY422045) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224739 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MPEG1 protein (Cat# TP324739). The protein was produced from HEK293T cells transfected with MPEG1 cDNA clone (Cat# RC224739) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MPEG1
• Synonyms: Mpg-1; MPG1; MPS1; P-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224739 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAACTTCAGGGCCACCATCCTCTTCTGGGCAGCGGCAGCATGGGCTAAATCAGGCAAGCCTTCGG
  GAGAGATGGACGAAGTTGGAGTTCAAAAATGCAAGAATGCCTTGAAACTACCTGTCCTGGAAGTCCTACC
  TGGAGGGGGCTGGGACAATCTGCGGAATGTGGACATGGGACGAGTTATGGAATTGACTTACTCCAACTGC
  AGGACAACAGAGGATGGACAGTATATCATCCCTGATGAAATCTTCACCATTCCCCAGAAACAGAGCAACC
  TGGAGATGAACTCAGAAATCCTGGAATCCTGGGCAAATTACCAGAGTAGCACCTCCTACTCCATCAACAC
  AGAACTCTCTCTTTTTTCCAAAGTCAATGGCAAGTTTTCCACTGAGTTCCAGAGGATGAAGACCCTCCAA
  GTGAAGGACCAAGCTATAACTACCCGAGTTCAGGTAAGAAACCTCGTCTACACAGTCAAAATCAACCCAA
  CTTTAGAGCTAAGCTCAGGTTTTAGGAAGGAACTCCTTGACATCTCTGACCGTCTAGAGAACAACCAGAC
  GAGGATGGCCACCTACCTGGCAGAACTCCTGGTGCTCAACTATGGCACCCACGTCACCACCAGTGTCGAC
  GCTGGGGCTGCTCTTATTCAGGAGGACCACCTCAGGGCCTCCTTCCTCCAAGACAGCCAGAGCAGTCGTA
  GTGCCGTGACCGCCTCTGCTGGACTTGCCTTTCAAAACACCGTGAACTTCAAATTTGAGGAAAACTATAC
  CTCGCAGAATGTCCTCACCAAGAGCTACCTCTCAAACCGAACCAACTCCAGGGTGCAGAGCATTGGAGGG
  GTTCCTTTTTACCCAGGCATCACCCTCCAGGCCTGGCAGCAGGGTATCACCAACCACCTGGTGGCCATCG
  ACCGCTCTGGCCTGCCGCTGCATTTCTTCATCAACCCCAACATGCTACCTGACTTGCCAGGCCCCCTGGT
  GAAGAAGGTGTCAAAGACAGTGGAAACTGCTGTGAAGCGCTATTATACATTCAACACCTACCCTGGCTGC
  ACAGATCTCAATTCTCCCAACTTCAATTTTCAGGCCAACACGGATGATGGCTCCTGCGAGGGGAAAATGA
  CCAACTTCTCTTTCGGTGGGGTTTATCAGGAATGCACTCAGCTCTCAGGGAATAGGGATGTCCTCCTCTG
  CCAAAAGTTGGAGCAGAAGAATCCACTCACTGGTGATTTCTCCTGCCCCTCTGGCTACTCCCCGGTGCAC
  CTGTTATCCCAGATCCACGAGGAGGGTTACAACCACCTGGAGTGTCATCGAAAGTGCACTCTCCTCGTCT
  TCTGCAAGACCGTGTGTGAAGATGTGTTCCAGGTGGCAAAAGCTGAATTTAGGGCTTTTTGGTGTGTGGC
  CAGCAGCCAAGTACCTGAAAACTCAGGACTGCTTTTTGGGGGCCTCTTCAGCAGCAAGAGCATAAACCCC
  ATGACAAATGCACAGTCATGCCCAGCCGGCTACTTTCCACTGAGACTCTTTGAAAACCTCAAGGTATGTG
  TTTCTCAGGACTATGAGTTGGGAAGCAGGTTTGCGGTCCCCTTTGGCGGGTTCTTTAGCTGCACAGTTGG
  GAACCCCCTGGTAGATCCTGCTATATCCAGAGATTTAGGGGCACTGTCTCTGAAAAAGTGCCCCGGGGGC
  TTCAGCCAGCACCCAGCCCTCATCAGCGATGGATGCCAAGTGTCCTATTGCGTCAAATCCGGGCTCTTCA
  CAGGAGGGTCCCTGCCCCCTGCCAGGCTCCCACCTTTCACCCGGCCACCCCTCATGAGTCAGGCTGCCAC
  CAATACTGTCATAGTGACCAATTCTGAGAATGCGAGATCCTGGATTAAAGACTCCCAGACCCACCAGTGG
  AGGCTGGGAGAACCGATAGAGCTGCGGAGGGCCATGAATGTCATCCATGGGGATGGTGGTGGTCTGTCAG
  GAGGGGCTGCAGCTGGGGTCACAGTGGGGGTCACCACCATTCTGGCTGTTGTTATCACCTTGGCCATCTA
  CGGCACCCGGAAGTTCAAGAAGAAAGCATATCAGGCAATTGAGGAAAGGCAGAGTTTGGTTCCAGGCACT
  GCAGCAACTGGAGACACCACTTACCAAGAGCAGGGGCAGAGTCCAGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224739 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNNFRATILFWAAAAWAKSGKPSGEMDEVGVQKCKNALKLPVLEVLPGGGWDNLRNVDMGRVMELTYSNC
  RTTEDGQYIIPDEIFTIPQKQSNLEMNSEILESWANYQSSTSYSINTELSLFSKVNGKFSTEFQRMKTLQ
  VKDQAITTRVQVRNLVYTVKINPTLELSSGFRKELLDISDRLENNQTRMATYLAELLVLNYGTHVTTSVD
  AGAALIQEDHLRASFLQDSQSSRSAVTASAGLAFQNTVNFKFEENYTSQNVLTKSYLSNRTNSRVQSIGG
  VPFYPGITLQAWQQGITNHLVAIDRSGLPLHFFINPNMLPDLPGPLVKKVSKTVETAVKRYYTFNTYPGC
  TDLNSPNFNFQANTDDGSCEGKMTNFSFGGVYQECTQLSGNRDVLLCQKLEQKNPLTGDFSCPSGYSPVH
  LLSQIHEEGYNHLECHRKCTLLVFCKTVCEDVFQVAKAEFRAFWCVASSQVPENSGLLFGGLFSSKSINP
  MTNAQSCPAGYFPLRLFENLKVCVSQDYELGSRFAVPFGGFFSCTVGNPLVDPAISRDLGALSLKKCPGG
  FSQHPALISDGCQVSYCVKSGLFTGGSLPPARLPPFTRPPLMSQAATNTVIVTNSENARSWIKDSQTHQW
  RLGEPIELRRAMNVIHGDGGGLSGGAAAGVTVGVTTILAVVITLAIYGTRKFKKKAYQAIEERQSLVPGT
  AATGDTTYQEQGQSPA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039396
• ORF Size: 2148 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039396.1, NP_001034485.1
• RefSeq Size: 4527 bp
• RefSeq ORF: 2151 bp
• Locus ID: 219972
• UniProt ID: Q2M385
• Cytogenetics: 11q12.1
• Protein Families: Transmembrane
• MW: 78.6 kDa
• Gene Summary: Plays a key role in the innate immune response following bacterial infection by inserting into the bacterial surface to form pores (By similarity). By breaching the surface of phagocytosed bacteria, allows antimicrobial effectors to enter the bacterial periplasmic space and degrade bacterial proteins such as superoxide dismutase sodC which contributes to bacterial virulence (By similarity). Shows antibacterial activity against a wide spectrum of Gram-positive, Gram-negative and acid-fast bacteria (PubMed:23753625, PubMed:26402460, PubMed:30609079). Reduces the viability of the intracytosolic pathogen L.monocytogenes by inhibiting acidification of the phagocytic vacuole of host cells which restricts bacterial translocation from the vacuole to the cytosol (By similarity). Required for the antibacterial activity of reactive oxygen species and nitric oxide (By similarity).[UniProtKB/Swiss-Prot Function]