ZFAND1 (NM_024699) Human Tagged ORF Clone
CAT#: RC224679
ZFAND1 (Myc-DDK-tagged)-Human zinc finger, AN1-type domain 1 (ZFAND1), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_024699" in other vectors (4)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | ZFAND1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC224679 representing NM_024699
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGTTGGACATCGGGCAGCACTGCCAGGTGGAGCATTGCCGGCAGCGAGATTTTCTTCCATTTG TGTGTGATGATTGTTCAGGAATATTTTGCCTTGAACACAGAAGCAGGGAGTCTCATGGTTGTCCTGAGGT GACTGTAATCAATGAGAGACTGAAGACAGATCAACATACATCTTACCCATGCTCTTTCAAAGACTGTGCT GAGAGAGAACTTGTGGCAGTTATATGTCCTTATTGTGAGAAGAATTTTTGCCTGAGACACCGTCATCAGT CAGATCATGAGTGTGAAAAACTGGAAATCCCAAAGCCTCGAATGGCTGCCACTCAGAAACTTGTTAAAGA CATTATTGATTCCAAGACAGGAGAAACAGCAAGTAAACGATGGAAAGGTGCCAAAAATAGTGAAACAGCT GCAAAGGTTGCATTGATGAAATTAAAGATGCATGCTGATGGCGATAAGTCATTACCACAGACAGAAAGAA TTTACTTTCAGGTTTTCTTACCTAAAGGGAGCAAAGAGAAGAGCAAACCAATGTTCTTTTGCCACCGATG GAGCATTGGAAAGGCCATAGACTTTGCCGCTTCTCTAGCCAGGCTTAAAAATGACAATAACAAATTTACA GCTAAGAAATTAAGGCTGTGTCACATTACTTCAGGAGAAGCCTTACCCTTGGATCATACTTTGGAAACCT GGATTGCTAAGGAGGATTGTCCTTTATATAATGGTGGAAATATAATCTTGGAATATCTTAATGATGAAGA ACAATTCTGTAAAAATGTTGAATCTTACTTGGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224679 representing NM_024699
Red=Cloning site Green=Tags(s) MAELDIGQHCQVEHCRQRDFLPFVCDDCSGIFCLEHRSRESHGCPEVTVINERLKTDQHTSYPCSFKDCA ERELVAVICPYCEKNFCLRHRHQSDHECEKLEIPKPRMAATQKLVKDIIDSKTGETASKRWKGAKNSETA AKVALMKLKMHADGDKSLPQTERIYFQVFLPKGSKEKSKPMFFCHRWSIGKAIDFAASLARLKNDNNKFT AKKLRLCHITSGEALPLDHTLETWIAKEDCPLYNGGNIILEYLNDEEQFCKNVESYLE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_024699 |
ORF Size | 804 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024699.3 |
RefSeq Size | 1793 bp |
RefSeq ORF | 807 bp |
Locus ID | 79752 |
UniProt ID | Q8TCF1 |
Cytogenetics | 8q21.13 |
Domains | ZnF_AN1 |
MW | 30.6 kDa |
Gene Summary | Plays a role in the regulation of cytoplasmic stress granules (SGs) turnover. SGs are dynamic and transient cytoplasmic ribonucleoprotein assemblies important for cellular protein homeostasis when protein production is suspended after acute exogenous stress (PubMed:29804830). Associates with SGs and is involved in the efficient and specific arsenite-induced clearance process of SGs through the recruitment of the ubiquitin-selective ATPase VCP and the 26S proteasome (PubMed:29804830). This process requires both complexes for efficient degradation of damaged ubiquitinated SG proteins during recovery from arsenite stress, and hence avoiding aberrant cytoplasmic SGs degradation via autophagy (PubMed:29804830).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC224679L3 | Lenti ORF clone of Human zinc finger, AN1-type domain 1 (ZFAND1), transcript variant 1, Myc-DDK-tagged |
USD 600.00 |
|
RC224679L4 | Lenti ORF clone of Human zinc finger, AN1-type domain 1 (ZFAND1), transcript variant 1, mGFP tagged |
USD 600.00 |
|
RG224679 | ZFAND1 (tGFP-tagged) - Human zinc finger, AN1-type domain 1 (ZFAND1), transcript variant 1 |
USD 500.00 |
|
SC312719 | ZFAND1 (untagged)-Human zinc finger, AN1-type domain 1 (ZFAND1), transcript variant 1 |
USD 330.00 |
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