USP11 (NM_004651) Human Tagged ORF Clone

CAT#: RC224592

USP11 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 11 (USP11)

Product Images

Circular map for RC224592

• 

  Western blot validation of overexpression lysate (Cat# LY417844) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224592 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: USP11
• Synonyms: UHX1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224592 representing NM_004651
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTAGCCCCGCGACTGTTTGGGGGGCTCTGCTTCCGTTTCCGGGACCAGAATCCGGAAGTGGCTG
  TTGAGGGGCGTCTTCCAATCTCGCACAGCTGCGTTGGCTGTAGAAGAGAACGGACGGCGATGGCGACGGT
  CGCAGCAAATCCAGCTGCTGCTGCGGCGGCTGTGGCGGCGGCAGCGGCGGTGACTGAGGATAGAGAGCCA
  CAGCACGAGGAGCTGCCAGGCCTGGACAGCCAGTGGCGCCAGATAGAAAACGGCGAGAGTGGGCGAGAAC
  GTCCACTGCGGGCCGGCGAAAGCTGGTTCCTTGTGGAGAAGCACTGGTATAAGCAGTGGGAGGCATACGT
  GCAGGGAGGGGACCAGGACTCCAGCACCTTCCCTGGCTGCATCAACAATGCCACACTCTTTCAAGATGAG
  ATAAACTGGCGCCTCAAGGAGGGACTGGTGGAAGGCGAGGATTATGTGCTGCTCCCAGCAGCTGCTTGGC
  ATTACCTGGTCAGCTGGTATGGTCTAGAGCATGGCCAGCCACCCATTGAACGCAAGGTCATAGAGCTGCC
  CAACATCCAGAAGGTCGAAGTGTACCCAGTAGAACTGCTGCTTGTCCGGCACAATGATTTGGGCAAATCT
  CACACTGTTCAGTTCAGCCATACCGATTCTATTGGCCTAGTATTGCGCACAGCTCGGGAGCGGTTTCTGG
  TGGAGCCCCAGGAAGACACTCGGCTTTGGGCCAAGAACTCAGAAGGCTCTTTGGATAGGTTGTATGACAC
  ACACATCACGGTTCTCGATGCGGCCCTTGAGACTGGGCAGTTGATCATCATGGAGACCCGCAAGAAAGAT
  GGCACTTGGCCCAGCGCACAGCTGCATGTCATGAACAACAACATGTCGGAAGAGGATGAGGACTTCAAGG
  GTCAGCCAGGCATCTGTGGCCTCACCAATCTGGGCAACACGTGCTTCATGAACTCGGCCCTGCAGTGCCT
  CAGCAATGTGCCACAGCTCACCGAGTACTTCCTCAACAACTGCTACCTGGAGGAGCTCAACTTCCGCAAC
  CCACTGGGCATGAAGGGTGAGATCGCAGAGGCCTATGCAGACCTGGTGAAGCAGGCGTGGTCTGGCCACC
  ACCGCTCCATTGTGCCACATGTGTTCAAGAACAAGGTTGGCCATTTTGCATCCCAATTTCTGGGCTACCA
  GCAGCATGACTCTCAGGAGCTGCTGTCATTCCTCCTGGACGGGCTGCATGAGGACCTTAATCGGGTGAAG
  AAGAAGGAGTATGTGGAGCTGTGCGATGCTGCTGGGCGACCGGATCAGGAGGTGGCACAGGAGGCATGGC
  AAAACCACAAACGGCGGAACGATTCTGTGATCGTGGACACTTTCCACGGCCTCTTCAAGTCCACGCTGGT
  GTGCCCCGATTGTGGCAATGTATCTGTGACCTTCGACCCCTTCTGCTACCTCAGTGTTCCACTGCCTATC
  AGCCACAAGAGGGTCTTGGAGGTCTTCTTTATCCCCATGGATCCGCGCCGCAAGCCAGAGCAGCACCGGC
  TCGTGGTCCCCAAGAAAGGCAAGATCTCGGATCTATGTGTGGCTCTGTCCAAACACACGGGCATCTCGCC
  AGAGAGGATGATGGTGGCTGATGTCTTCAGTCACCGCTTCTATAAGCTCTATCAGCTAGAGGAGCCTCTG
  AGCAGCATCTTGGACCGTGATGATATCTTCGTCTATGAGGTGTCAGGTCGCATTGAGGCCATTGAGGGCT
  CAAGAGAGGACATCGTGGTTCCTGTCTACCTGCGGGAGCGCACCCCTGCCCGTGACTACAACAACTCCTA
  CTACGGCCTGATGCTTTTTGGACACCCCCTCCTGGTATCAGTGCCCCGGGACCGCTTCACCTGGGAGGGC
  CTGTATAACGTCCTGATGTACCGGCTCTCACGCTACGTGACCAAACCCAACTCAGATGATGAGGACGATG
  GGGATGAGAAAGAAGATGACGAGGAGGATAAAGATGACGTCCCTGGGCCCTCAACTGGGGGCAGCCTCCG
  AGACCCTGAGCCAGAGCAGGCTGGGCCCAGCTCTGGAGTCACGAACAGGTGCCCGTTCCTCCTGGACAAT
  TGCCTTGGCACATCTCAGTGGCCCCCAAGGCGACGACGCAAGCAGCTGTTCACCCTGCAGACGGTGAACT
  CCAATGGGACCAGCGACCGCACAACCTCCCCTGAAGAAGTCCATGCCCAGCCGTACATTGCTATCGACTG
  GGAGCCAGAGATGAAGAAGCGTTACTATGACGAGGTAGAGGCTGAGGGCTACGTGAAGCATGACTGCGTC
  GGGTACGTGATGAAGAAGGCTCCCGTGCGGCTGCAGGAGTGCATTGAGCTCTTCACCACTGTGGAGACCC
  TGGAGAAGGAAAACCCCTGGTACTGCCCTTCCTGCAAGCAGCACCAGCTGGCAACCAAGAAGCTGGACCT
  GTGGATGCTGCCGGAGATTCTCATCATCCACCTGAAACGCTTTTCCTACACCAAGTTCTCCCGAGAGAAG
  CTGGACACCCTCGTGGAGTTTCCTATCCGGGACCTGGACTTCTCTGAGTTTGTCATCCAGCCACAGAATG
  AGTCGAATCCGGAGCTGTACAAATATGACCTCATCGCGGTTTCCAACCATTATGGGGGCATGCGTGATGG
  ACACTACACAACATTTGCCTGCAACAAGGACAGCGGCCAGTGGCACTACTTTGATGACAACAGCGTCTCC
  CCTGTCAATGAGAATCAGATCGAGTCCAAGGCAGCCTATGTCCTCTTCTACCAACGCCAGGACGTGGCGC
  GACGCCTGCTGTCCCCGGCCGGCTCATCTGGCGCCCCAGCCTCCCCTGCCTGCAGCTCCCCACCCAGCTC
  TGAGTTCATGGATGTTAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224592 representing NM_004651
  Red=Cloning site Green=Tags(s)
  
  MAVAPRLFGGLCFRFRDQNPEVAVEGRLPISHSCVGCRRERTAMATVAANPAAAAAAVAAAAAVTEDREP
  QHEELPGLDSQWRQIENGESGRERPLRAGESWFLVEKHWYKQWEAYVQGGDQDSSTFPGCINNATLFQDE
  INWRLKEGLVEGEDYVLLPAAAWHYLVSWYGLEHGQPPIERKVIELPNIQKVEVYPVELLLVRHNDLGKS
  HTVQFSHTDSIGLVLRTARERFLVEPQEDTRLWAKNSEGSLDRLYDTHITVLDAALETGQLIIMETRKKD
  GTWPSAQLHVMNNNMSEEDEDFKGQPGICGLTNLGNTCFMNSALQCLSNVPQLTEYFLNNCYLEELNFRN
  PLGMKGEIAEAYADLVKQAWSGHHRSIVPHVFKNKVGHFASQFLGYQQHDSQELLSFLLDGLHEDLNRVK
  KKEYVELCDAAGRPDQEVAQEAWQNHKRRNDSVIVDTFHGLFKSTLVCPDCGNVSVTFDPFCYLSVPLPI
  SHKRVLEVFFIPMDPRRKPEQHRLVVPKKGKISDLCVALSKHTGISPERMMVADVFSHRFYKLYQLEEPL
  SSILDRDDIFVYEVSGRIEAIEGSREDIVVPVYLRERTPARDYNNSYYGLMLFGHPLLVSVPRDRFTWEG
  LYNVLMYRLSRYVTKPNSDDEDDGDEKEDDEEDKDDVPGPSTGGSLRDPEPEQAGPSSGVTNRCPFLLDN
  CLGTSQWPPRRRRKQLFTLQTVNSNGTSDRTTSPEEVHAQPYIAIDWEPEMKKRYYDEVEAEGYVKHDCV
  GYVMKKAPVRLQECIELFTTVETLEKENPWYCPSCKQHQLATKKLDLWMLPEILIIHLKRFSYTKFSREK
  LDTLVEFPIRDLDFSEFVIQPQNESNPELYKYDLIAVSNHYGGMRDGHYTTFACNKDSGQWHYFDDNSVS
  PVNENQIESKAAYVLFYQRQDVARRLLSPAGSSGAPASPACSSPPSSEFMDVN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004651
• ORF Size: 2889 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004651.3, NP_004642.2
• RefSeq Size: 3300 bp
• RefSeq ORF: 2892 bp
• Locus ID: 8237
• UniProt ID: P51784
• Cytogenetics: Xp11.3
• Domains: UCH, DUSP
• Protein Families: Druggable Genome, Protease
• MW: 109.6 kDa
• Gene Summary: Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. This gene encodes a deubiquitinating enzyme which lies in a gene cluster on chromosome Xp11.23 [provided by RefSeq, Jul 2008]