Lamin B Receptor (LBR) (NM_194442) Human Tagged ORF Clone

CAT#: RC224569

LBR (Myc-DDK-tagged)-Human lamin B receptor (LBR), transcript variant 2

Product Images

Circular map for RC224569

• 

  Western blot validation of overexpression lysate (Cat# LY405131) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224569 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lamin B Receptor
• Synonyms: C14SR; DHCR14B; LMN2R; PHA; PHASK; TDRD18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224569 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAAGTAGGAAATTTGCCGATGGTGAAGTGGTAAGAGGTCGATGGCCTGGGAGTTCACTTTATTATG
  AAGTAGAAATTCTGAGCCACGACAGCACCTCCCAGCTTTACACTGTGAAGTATAAAGATGGAACAGAGCT
  TGAATTGAAAGAGAATGATATTAAGCCTTTAACTTCCTTTAGGCAAAGGAAAGGTGGCTCAACTTCCAGT
  TCCCCTTCCAGACGCCGAGGGAGTCGATCAAGGTCACGCTCCCGATCCCCTGGTCGACCACCTAAAAGTG
  CCCGCCGATCTGCTTCTGCTTCCCACCAGGCCGACATTAAGGAAGCAAGGAGGGAAGTGGAAGTTAAATT
  GACTCCGCTGATTCTGAAGCCATTTGGAAATAGCATCAGCAGATATAATGGGGAGCCTGAGCATATTGAG
  AGAAATGACGCACCTCATAAAAATACACAGGAAAAATTCAGTTTGTCACAAGAAAGCAGTTACATAGCAA
  CACAGTATAGCCTTCGTCCAAGAAGAGAAGAAGTCAAATTAAAAGAAATAGATTCTAAGGAAGAAAAATA
  CGTTGCAAAAGAACTGGCAGTGAGAACCTTTGAAGTGACCCCCATCCGGGCAAAGGACTTGGAGTTTGGA
  GGAGTACCTGGTGTGTTTCTCATCATGTTTGGCCTGCCTGTGTTCCTCTTCCTGTTGCTGTTGATGTGTA
  AACAGAAAGATCCCAGTCTTCTGAATTTCCCTCCTCCTTTGCCAGCTTTGTATGAGTTATGGGAAACCAG
  AGTATTTGGGGTCTACCTCCTGTGGTTTTTGATTCAAGTCCTGTTCTACCTACTGCCAATTGGAAAGGTT
  GTAGAAGGAACGCCTCTTATTGATGGAAGAAGACTCAAGTATAGATTAAATGGATTCTATGCTTTTATCC
  TGACATCTGCAGTCATCGGAACATCTCTCTTCCAGGGCGTAGAGTTTCATTACGTGTACAGTCATTTTCT
  TCAGTTTGCACTTGCGGCCACTGTTTTTTGTGTGGTCTTGAGTGTGTATCTCTACATGCGCTCTTTGAAA
  GCGCCCCGGAATGACCTGTCGCCTGCCAGCTCTGGAAATGCTGTCTATGATTTCTTCATTGGCCGTGAAT
  TAAACCCTCGAATTGGTACTTTTGATCTCAAATACTTTTGTGAATTGCGCCCCGGATTGATTGGATGGGT
  GGTTATTAACTTGGTGATGCTTTTGGCTGAAATGAAAATACAGGACCGCGCTGTTCCATCCTTGGCCATG
  ATTTTAGTTAATAGTTTCCAGCTTCTCTATGTGGTGGATGCTCTCTGGAATGAGGAAGCGTTGTTGACGA
  CCATGGACATCATCCACGATGGATTTGGATTCATGCTGGCTTTTGGAGACTTGGTGTGGGTTCCCTTTAT
  TTACAGCTTCCAAGCCTTTTATTTAGTCAGTCATCCAAATGAAGTGTCTTGGCCAATGGCTTCTCTAATT
  ATTGTTCTGAAACTTTGTGGTTATGTAATCTTCCGAGGTGCAAATTCTCAGAAAAATGCATTCCGGAAAA
  ATCCCAGTGATCCAAAGCTTGCACATTTAAAAACCATTCATACTTCAACGGGAAAAAATCTTCTAGTTTC
  TGGATGGTGGGGCTTTGTTCGCCACCCCAATTACTTGGGTGATCTCATCATGGCCTTGGCGTGGTCCCTC
  CCATGTGGTTTTAACCACATTCTGCCTTATTTCTACATAATTTATTTCACCATGTTGCTTGTCCACCGAG
  AAGCTCGTGACGAGTACCACTGTAAGAAGAAATACGGCGTGGCTTGGGAAAAGTACTGTCAGCGTGTGCC
  CTACCGTATATTTCCATACATCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224569 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPSRKFADGEVVRGRWPGSSLYYEVEILSHDSTSQLYTVKYKDGTELELKENDIKPLTSFRQRKGGSTSS
  SPSRRRGSRSRSRSRSPGRPPKSARRSASASHQADIKEARREVEVKLTPLILKPFGNSISRYNGEPEHIE
  RNDAPHKNTQEKFSLSQESSYIATQYSLRPRREEVKLKEIDSKEEKYVAKELAVRTFEVTPIRAKDLEFG
  GVPGVFLIMFGLPVFLFLLLLMCKQKDPSLLNFPPPLPALYELWETRVFGVYLLWFLIQVLFYLLPIGKV
  VEGTPLIDGRRLKYRLNGFYAFILTSAVIGTSLFQGVEFHYVYSHFLQFALAATVFCVVLSVYLYMRSLK
  APRNDLSPASSGNAVYDFFIGRELNPRIGTFDLKYFCELRPGLIGWVVINLVMLLAEMKIQDRAVPSLAM
  ILVNSFQLLYVVDALWNEEALLTTMDIIHDGFGFMLAFGDLVWVPFIYSFQAFYLVSHPNEVSWPMASLI
  IVLKLCGYVIFRGANSQKNAFRKNPSDPKLAHLKTIHTSTGKNLLVSGWWGFVRHPNYLGDLIMALAWSL
  PCGFNHILPYFYIIYFTMLLVHREARDEYHCKKKYGVAWEKYCQRVPYRIFPYIY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_194442
• ORF Size: 1845 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_194442.2
• RefSeq Size: 3827 bp
• RefSeq ORF: 1848 bp
• Locus ID: 3930
• UniProt ID: Q14739
• Cytogenetics: 1q42.12
• Protein Families: Druggable Genome, Transmembrane
• MW: 70.7 kDa
• Gene Summary: The protein encoded by this gene belongs to the ERG4/ERG24 family. It localized in the nuclear envelope inner membrane and anchors the lamina and the heterochromatin to the membrane. It may mediate interaction between chromatin and lamin B. Mutations of this gene has been associated with autosomal recessive HEM/Greenberg skeletal dysplasia. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]