EXOC6B (NM_015189) Human Tagged ORF Clone

CAT#: RC224563

EXOC6B (Myc-DDK-tagged)-Human exocyst complex component 6B (EXOC6B)

Product Images

Circular map for RC224563

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  Western blot validation of overexpression lysate (Cat# LY414687) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224563 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified EXOC6B protein (Cat# TP324563). The protein was produced from HEK293T cells transfected with EXOC6B cDNA clone (Cat# RC224563) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EXOC6B
• Synonyms: SEC15B; SEC15L2; SEMDJL3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224563 representing NM_015189
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGGGTAAGATGGCGGAGGCGGAGAGCCTGGAGACAGCGGCAGAGCACGAGCGGATCCTGCGAG
  AGATCGAGAGCACTGACACGGCCTGCATCGGGCCCACGCTCAGGTCTGTTTATGATGGTGAAGAACATGG
  ACGTTTCATGGAGAAGCTTGAAACTCGTATCCGTAATCACGACCGAGAAATTGAGAAAATGTGCAACTTT
  CATTACCAGGGCTTTGTGGACTCTATAACTGAACTGCTGAAAGTGAGAGGAGAAGCCCAGAAACTCAAAA
  ATCAAGTGACGGATACTAATAGAAAACTACAACATGAGGGAAAGGAACTGGTAATAGCAATGGAAGAGCT
  GAAGCAGTGTCGACTACAACAGAGAAATATTTCTGCCACTGTTGATAAATTAATGCTGTGTCTTCCAGTC
  CTAGAGATGTACAGCAAACTGAGGGACCAGATGAAAACTAAAAGGCATTATCCTGCACTGAAAACTCTGG
  AACATCTAGAGCATACCTACCTGCCTCAAGTAAGCCACTATCGATTCTGCAAGGTGATGGTGGACAACAT
  CCCCAAGCTTCGAGAAGAAATAAAAGATGTTTCTATGTCCGATCTCAAAGACTTTCTGGAGAGCATCCGC
  AAACATTCAGACAAAATTGGAGAGACTGCCATGAAGCAAGCCCAGCAGCAAAGAAACCTGGATAACATCG
  TCTTGCAACAACCCAGAATAGGTAGCAAGAGGAAATCTAAGAAAGATGCATATATAATCTTTGATACAGA
  GATAGAAAGTACTAGTCCGAAGTCTGAACAGGATTCAGGAATTCTGGATGTTGAAGACGAGGAAGATGAT
  GAAGAGGTACCTGGGGCCCAAGATTTGGTGGATTTCTCTCCAGTTTATCGATGTCTACATATATATTCTG
  TCCTGGGTGCCCGGGAAACATTTGAGAATTACTACCGAAAACAGAGGCGAAAACAGGCTCGTTTGGTACT
  TCAACCTCCATCTAACATGCATGAAACTTTAGATGGCTACAGGAAGTATTTTAATCAAATTGTAGGCTTT
  TTTGTGGTTGAAGATCACATTTTACATACAACCCAGGGCTTAGTAAATAGAGCCTACATTGATGAACTGT
  GGGAAATGGCACTTTCAAAAACCATCGCAGCACTCCGTACCCACTCGTCTTACTGTTCTGATCCAAACCT
  TGTGTTAGATTTGAAGAACCTCATTGTGCTTTTTGCTGACACACTTCAGGTGTATGGTTTCCCTGTAAAT
  CAGCTTTTTGACATGCTGTTAGAAATCAGAGACCAATATAGTGAAACTCTGCTAAAGAAGTGGGCAGGTA
  TTTTCAGAAACATACTTGATTCTGACAACTACAGTCCTATACCAGTAACAAGTGAAGAGATGTACAAAAA
  GGTGGTAGGACAATTCCCATTTCAAGATATAGAACTGGAAAAGCAACCATTTCCAAAAAAGTTTCCTTTC
  TCTGAATTTGTGCCAAAAGTTTACAACCAAATTAAAGAATTTATCTACGCTTGTCTGAAGTTTTCAGAAG
  ATCTTCATCTAAGCTCAACTGAAGTTGATGACATGATTCGGAAATCAACAAACCTGTTGCTAACCAGGAC
  TCTGAGCAACTCTCTGCAGAATGTAATTAAAAGGAAGAATATTGGGCTTACTGAGCTTGTTCAGATTATT
  ATCAATACAACACATTTGGAGAAATCCTGTAAGTACTTGGAAGAATTTATCACCAACATCACTAATGTGC
  TTCCAGAGACAGTTCACACTACCAAGCTCTATGGCACCACAACTTTTAAGGATGCTAGACATGCAGCTGA
  AGAAGAGATTTATACCAACTTAAACCAGAAGATTGACCAGTTCCTACAGCTGGCAGACTATGACTGGATG
  ACCGGAGATTTGGGCAACAAAGCTAGTGATTACCTGGTAGACCTCATTGCCTTTCTTCGTAGCACCTTTG
  CTGTATTCACACACCTTCCTGGAAAGGTGGCCCAGACAGCGTGTATGTCAGCTTGCAAGCATTTAGCCAC
  ATCCTTGATGCAACTTTTGTTGGAAGCTGAAGTGCGGCAGCTCACCTTGGGAGCATTACAGCAGTTCAAC
  TTGGACGTCAGAGAATGTGAACAGTTTGCCAGATCCGGCCCGGTGCCTGGGTTCCAGGAGGACACGCTGC
  AGTTGGCCTTCATCGACTTGAGACAACTTTTGGATCTTTTCATCCAGTGGGATTGGTCAACCTACCTTGC
  TGACTATGGTCAGCCCAACTGCAAGTACCTCCGGGTAAACCCAGTGACTGCTCTGACCCTGCTTGAGAAG
  ATGAAGGATACTAGCCGCAAGAACAACATGTTTGCACAGTTTCGGAAAAATGAGCGAGACAAGCAGAAAC
  TCATTGACACCGTGGCCAAGCAGCTCCGAGGACTCATCAGCAGCCACCACTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224563 representing NM_015189
  Red=Cloning site Green=Tags(s)
  
  MERGKMAEAESLETAAEHERILREIESTDTACIGPTLRSVYDGEEHGRFMEKLETRIRNHDREIEKMCNF
  HYQGFVDSITELLKVRGEAQKLKNQVTDTNRKLQHEGKELVIAMEELKQCRLQQRNISATVDKLMLCLPV
  LEMYSKLRDQMKTKRHYPALKTLEHLEHTYLPQVSHYRFCKVMVDNIPKLREEIKDVSMSDLKDFLESIR
  KHSDKIGETAMKQAQQQRNLDNIVLQQPRIGSKRKSKKDAYIIFDTEIESTSPKSEQDSGILDVEDEEDD
  EEVPGAQDLVDFSPVYRCLHIYSVLGARETFENYYRKQRRKQARLVLQPPSNMHETLDGYRKYFNQIVGF
  FVVEDHILHTTQGLVNRAYIDELWEMALSKTIAALRTHSSYCSDPNLVLDLKNLIVLFADTLQVYGFPVN
  QLFDMLLEIRDQYSETLLKKWAGIFRNILDSDNYSPIPVTSEEMYKKVVGQFPFQDIELEKQPFPKKFPF
  SEFVPKVYNQIKEFIYACLKFSEDLHLSSTEVDDMIRKSTNLLLTRTLSNSLQNVIKRKNIGLTELVQII
  INTTHLEKSCKYLEEFITNITNVLPETVHTTKLYGTTTFKDARHAAEEEIYTNLNQKIDQFLQLADYDWM
  TGDLGNKASDYLVDLIAFLRSTFAVFTHLPGKVAQTACMSACKHLATSLMQLLLEAEVRQLTLGALQQFN
  LDVRECEQFARSGPVPGFQEDTLQLAFIDLRQLLDLFIQWDWSTYLADYGQPNCKYLRVNPVTALTLLEK
  MKDTSRKNNMFAQFRKNERDKQKLIDTVAKQLRGLISSHHS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015189
• ORF Size: 2433 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015189.3
• RefSeq Size: 2594 bp
• RefSeq ORF: 2436 bp
• Locus ID: 23233
• UniProt ID: Q9Y2D4
• Cytogenetics: 2p13.2
• MW: 94 kDa
• Gene Summary: This gene encodes a protein which is a part of the evolutionarily conserved exocyst, a multimeric protein complex necessary for exocytosis, which in turn, is crucial for cell growth, polarity and migration. Disruption of this gene may be associated with phenotypes exhibiting multiple symptoms including intellectual disability and developmental delay (DD). [provided by RefSeq, Jun 2016]