CC137 (CCDC137) (NM_199287) Human Tagged ORF Clone

CAT#: RC224420

CCDC137 (Myc-DDK-tagged)-Human coiled-coil domain containing 137 (CCDC137)

Product Images

Circular map for RC224420

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  Western blot validation of overexpression lysate (Cat# LY404608) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224420 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CC137
• Synonyms: RaRF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224420 representing NM_199287
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGAGCTGGTCGCGGAGCAGCGGTGTCCAGGGTGCAGGCGGGTCCTGGGAGTCCCCGGCGAGCGC
  GGGGGCGGCAGCAAGTGCAGCCGCTGGGGAAGCAGCGCCCAGCCCCGTGGCCCGGGCTTCGCAGCAAAGA
  GAAGAAGAAAGTGAACTGCAAGCCCAAGAACCAGGACGAACAGGAGATTCCTTTCCGGCTCCGGGAGATT
  ATGAGGAGCCGCCAAGAGATGAAAAACCCGATCAGTAACAAGAAGAGGAAGAAAGCAGCCCAGGTGACCT
  TCAGAAAGACATTGGAGAAGGAAGCAAAGGGAGAGGAGCCCGACATCGCAGTCCCCAAGTTCAAACAGAG
  GAAGGGGGAGTCCGACGGGGCCTATATCCACCGCATGCAGCAAGAGGCCCAGCATGTGCTGTTCCTCAGC
  AAGAACCAGGCCATCCGGCAGCCAGAGGTGCAGGCAGCTCCCAAGGAGAAGTCTGAGCAGAAAAAAGCAA
  AAAAAGCGTTCCAGAAGCGGCGACTAGATAAAGTCCGACGGAAAAAGGAGGAAAAGGCGGCAGACAGGCT
  GGAGCAGGAGTTGCTCCGAGACACGGTGAAGTTTGGTGAGGTTGTCCTGCAGCCCCCAGAGCTGACTGCC
  AGGCCCCAGAGGAGCGTAAGCAAGGACCAGCCTGGCAGGAGATCGCAGATGCTGCGGATGCTTCTGAGCC
  CCGGTGGTGTGTCCCAGCCTCTGACCGCCTCCCTGGCCCGCCAGCGGATTGTGGAGGAGGAGAGAGAGCG
  GGCCGTGCAGGCCTACAGAGCGTTGAAGCAGCGGCAGCAGCAGCTGCACGGGGAGCGACCCCACCTCACT
  TCCCGGAAGAAGCCAGAGCCGCAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224420 representing NM_199287
  Red=Cloning site Green=Tags(s)
  
  MAGAGRGAAVSRVQAGPGSPRRARGRQQVQPLGKQRPAPWPGLRSKEKKKVNCKPKNQDEQEIPFRLREI
  MRSRQEMKNPISNKKRKKAAQVTFRKTLEKEAKGEEPDIAVPKFKQRKGESDGAYIHRMQQEAQHVLFLS
  KNQAIRQPEVQAAPKEKSEQKKAKKAFQKRRLDKVRRKKEEKAADRLEQELLRDTVKFGEVVLQPPELTA
  RPQRSVSKDQPGRRSQMLRMLLSPGGVSQPLTASLARQRIVEEERERAVQAYRALKQRQQQLHGERPHLT
  SRKKPEPQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199287
• ORF Size: 867 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199287.2, NP_954981.1
• RefSeq Size: 2109 bp
• RefSeq ORF: 870 bp
• Locus ID: 339230
• UniProt ID: Q6PK04
• Cytogenetics: 17q25.3
• MW: 33.1 kDa