PAXBP1 (NM_016631) Human Tagged ORF Clone

CAT#: RC224365

PAXBP1 (Myc-DDK-tagged)-Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 1

Product Images

Circular map for RC224365

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PAXBP1
• Synonyms: BM020; C21orf66; FSAP105; GCFC; GCFC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224365 representing NM_016631
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCGAAAGGCCCGGCGGGTGAACGTGCGCAAGCGGAACGACTCCGAAGAGGAAGAGCGGGAACGCG
  ATGAGGAGCAGGAGCCGCCGCCGTTGTTGCCGCCGCCGGGCACGGGCGAAGAGGCGGGCCCCGGTGGCGG
  CGACAGGGCCCCTGGCGGGGAGTCGCTGCTGGGCCCGGGGCCGTCGCCGCCTTCCGCGCTGACCCCGGGC
  CTCGGGGCTGAGGCCGGGGGCGGCTTCCCCGGCGGCGCGGAGCCCGGCAACGGGCTGAAGCCGCGCAAGA
  GGCCTCGCGAGAACAAAGAGGTGCCCCGGGCCAGCCTGCTCAGCTTCCAGGACGAGGAGGAAGAAAATGA
  AGAAGTTTTCAAAGTGAAGAAATCAAGTTATAGCAAAAAGATAGTAAAATTGCTCAAGAAGGAATATAAA
  GAAGATCTTGAAAAATCGAAGATTAAGACAGAACTCAACTCATCAGCTGAAAGTGAACAACCTTTGGACA
  AAACAGGACATGTTAAGGATACAAATCAAGAAGATGGAGTTATCATCAGTGAACATGGTGAAGATGAAAT
  GGATATGGAAAGTGAAAAAGAGGAAGAAAAGCCAAAGACTGGTGGAGCTTTTTCAAATGCTTTATCTTCA
  TTGAATGTTCTTCGTCCAGGAGAAATTCCAGATGCAGCTTTTATACATGCTGCAAGGAAAAAGCGCCAAA
  TGGCCCGAGAATTGGGAGATTTCACTCCTCATGATAATGAGCCTGGTAAAGGCCGCCTTGTTAGAGAAGA
  TGAGAATGATGCCAGTGATGATGAAGATGACGATGAGAAACGCCGGATAGTTTTTTCTGTGAAAGAAAAG
  TCACAAAGACAAAAAATTGCTGAGGAAATAGGAATTGAGGGGAGTGATGATGATGCTTTAGTAACTGGAG
  AACAGGATGAAGAGCTCAGCCGATGGGAACAGGAGCAGATAAGGAAAGGAATTAATATCCCTCAGGTTCA
  AGCCAGTCAACCCGCAGAAGTGAATATGTACTACCAGAACACTTACCAGACAATGCCTTACGGCTCATCC
  TATGGCATTCCTTATAGTTATACGGCCTATGGATCATCAGATGCCAAATCTCAAAAAACAGATAATACAG
  TCCCTTTCAAAACTCCCAGTAATGAGATGACTCCCGTTACTATTGATTTGGTAAAGAAACAGCTTAAAGA
  CAGGTTGGACTCCATGAAAGAATTGCACAAAACAAATCGACAGCAGCATGAGAAACATCTGCAAAGCCGA
  GTGGACTCTACCAGGGCTATTGAAAGATTAGAAGGGTCTTCTGGGGGTATTGGTGAACGGTATAAATTTT
  TGCAAGAAATGCGAGGGTATGTCCAAGACTTGCTTGAGTGTTTCAGTGAAAAGGTGCCACTGATTAATGA
  ACTTGAATCAGCAATACATCAGCTGTACAAACAGCGAGCTTCCCGCCTTGTCCAAAGACGACAAGATGAT
  ATTAAAGATGAATCTTCGGAGTTTTCAAGCCATTCAAACAAAGCTCTGATGGCACCAAATCTTGACTCCT
  TTGGACGCGATCGGGCACTGTATCAAGAGCATGCAAAACGTCGCATTGCAGAGCGGGAGGCCAGGAGGAC
  TCGTCGTAGACAAGCCAGAGAACAAACCGGTAAGATGGCAGATCACCTTGAAGGCCTTTCCAGTGATGAT
  GAAGAAACTTCTACAGATATTACTAATTTCAATCTGGAAAAAGATCGAATTTCAAAAGAATCCGGCAAAG
  TTTTTGAAGATGTCCTTGAAAGTTTCTATTCAATTGACTGTATTAAATCACAGTTTGAAGCATGGCGTTC
  AAAATACTACACATCCTACAAAGATGCTTACATTGGCCTTTGTTTGCCAAAATTATTCAACCCCCTCATA
  CGACTTCAGCTCCTCACTTGGACTCCTCTTGAGGCAAAATGTCGTGACTTTGAGAATATGCTGTGGTTTG
  AATCTTTGCTGTTTTATGGTTGTGAAGAACGAGAGCAAGAAAAAGATGATGTAGATGTTGCCCTACTACC
  TACCATTGTGGAAAAGGTGATTCTTCCTAAACTAACAGTGATAGCTGAAAATATGTGGGACCCTTTTTCT
  ACAACACAGACTTCAAGAATGGTGGGAATTACACTAAAATTAATCAATGGATATCCTTCAGTAGTGAATG
  CAGAAAATAAAAATACACAGGTATACCTAAAGGCACTTTTATTGAGAATGAGAAGAACTTTAGATGATGA
  TGTATTTATGCCCTTATATCCCAAAAATGTCTTAGAAAATAAAAATTCTGGGCCTTACTTGTTTTTTCAA
  CGACAGTTTTGGTCTTCAGTTAAGCTGTTAGGCAATTTTCTTCAGTGGTATGGCATTTTCTCAAATAAAA
  CTCTGCAAGAGTTATCAATAGATGGTTTATTAAATCGATATATTCTCATGGCTTTTCAGAATTCAGAATA
  TGGAGATGACAGCATCAAAAAAGCCCAAAATGTAATCAATTGTTTCCCCAAACAATGGTTCATGAATCTG
  AAAGGAGAAAGGACTATTTCTCAGTTAGAAAACTTTTGCCGATACCTTGTACACTTAGCGGATACAATTT
  ATAGAAATAGTATCGGGTGTTCTGATGTGGAAAAAAGAAATGCAAGGGAAAACATAAAACAGATAGTAAA
  ACTCCTTGCAAGTGTTCGAGCTTTGGATCATGCTATGTCTGTTGCAAGTGACCACAATGTGAAAGAATTT
  AAGTCTTTGATCGAAGGAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224365 representing NM_016631
  Red=Cloning site Green=Tags(s)
  
  MFRKARRVNVRKRNDSEEEERERDEEQEPPPLLPPPGTGEEAGPGGGDRAPGGESLLGPGPSPPSALTPG
  LGAEAGGGFPGGAEPGNGLKPRKRPRENKEVPRASLLSFQDEEEENEEVFKVKKSSYSKKIVKLLKKEYK
  EDLEKSKIKTELNSSAESEQPLDKTGHVKDTNQEDGVIISEHGEDEMDMESEKEEEKPKTGGAFSNALSS
  LNVLRPGEIPDAAFIHAARKKRQMARELGDFTPHDNEPGKGRLVREDENDASDDEDDDEKRRIVFSVKEK
  SQRQKIAEEIGIEGSDDDALVTGEQDEELSRWEQEQIRKGINIPQVQASQPAEVNMYYQNTYQTMPYGSS
  YGIPYSYTAYGSSDAKSQKTDNTVPFKTPSNEMTPVTIDLVKKQLKDRLDSMKELHKTNRQQHEKHLQSR
  VDSTRAIERLEGSSGGIGERYKFLQEMRGYVQDLLECFSEKVPLINELESAIHQLYKQRASRLVQRRQDD
  IKDESSEFSSHSNKALMAPNLDSFGRDRALYQEHAKRRIAEREARRTRRRQAREQTGKMADHLEGLSSDD
  EETSTDITNFNLEKDRISKESGKVFEDVLESFYSIDCIKSQFEAWRSKYYTSYKDAYIGLCLPKLFNPLI
  RLQLLTWTPLEAKCRDFENMLWFESLLFYGCEEREQEKDDVDVALLPTIVEKVILPKLTVIAENMWDPFS
  TTQTSRMVGITLKLINGYPSVVNAENKNTQVYLKALLLRMRRTLDDDVFMPLYPKNVLENKNSGPYLFFQ
  RQFWSSVKLLGNFLQWYGIFSNKTLQELSIDGLLNRYILMAFQNSEYGDDSIKKAQNVINCFPKQWFMNL
  KGERTISQLENFCRYLVHLADTIYRNSIGCSDVEKRNARENIKQIVKLLASVRALDHAMSVASDHNVKEF
  KSLIEGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016631
• ORF Size: 2751 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016631.4
• RefSeq Size: 2903 bp
• RefSeq ORF: 2754 bp
• Locus ID: 94104
• UniProt ID: Q9Y5B6
• Cytogenetics: 21q22.11
• Protein Families: Transcription Factors
• MW: 104.6 kDa
• Gene Summary: This gene encodes a protein that may bind to GC-rich DNA sequences, which suggests its involvement in the regulation of transcription. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jun 2009]