GDAP2 (NM_017686) Human Tagged ORF Clone

CAT#: RC224299

GDAP2 (Myc-DDK-tagged)-Human ganglioside induced differentiation associated protein 2 (GDAP2), transcript variant 1

Product Images

Circular map for RC224299

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  Western blot validation of overexpression lysate (Cat# LY413601) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224299 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GDAP2 protein (Cat# TP324299). The protein was produced from HEK293T cells transfected with GDAP2 cDNA clone (Cat# RC224299) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GDAP2
• Synonyms: MACROD3; SCAR27
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224299 representing NM_017686
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCCTTAGGTGCACCTTCCCAGTTTGTGGATGTGGATACACTACCAAGCTGGGGTGACTCATGCC
  AAGATGAATTAAATTCCTCTGATACTACAGCTGAAATATTTCAGGAAGACACTGTTCGATCACCTTTTCT
  TTATAATAAGGACGTCAATGGAAAAGTGGTTCTTTGGAAAGGAGATGTGGCATTACTGAACTGTACAGCC
  ATTGTGAATACCAGCAATGAAAGTCTCACAGATAAGAATCCTGTGTCAGAAAGTATCTTCATGCTTGCAG
  GGCCTGATTTGAAGGAAGATCTCCAGAAACTTAAAGGGTGCCGAACAGGTGAAGCAAAATTGACAAAAGG
  ATTCAATCTAGCTGCCCGGTTCATCATTCACACAGTGGGACCTAAATATAAAAGCCGCTATCGCACAGCA
  GCTGAGAGTTCCCTTTATAGCTGCTACAGAAACGTACTTCAACTAGCAAAAGAGCAGTCAATGTCTTCTG
  TTGGCTTCTGTGTCATCAATTCTGCAAAACGTGGTTATCCTTTAGAGGATGCAACACACATAGCACTTCG
  CACTGTAAGAAGATTCCTAGAGATTCATGGGGAAACCATTGAAAAAGTAGTATTTGCTGTCTCTGATCTT
  GAAGAGGGTACTTACCAAAAGCTGCTACCTCTCTACTTCCCAAGGTCATTAAAAGAGGAGAATCGATCAT
  TGCCCTACCTACCTGCAGATATTGGAAATGCAGAAGGGGAGCCTGTGGTACCTGAACGACAGATTAGAAT
  AAGTGAGAAACCTGGTGCTCCAGAAGATAACCAAGAAGAGGAGGATGAAGGCTTGGGAGTTGATCTCTCT
  TTCATTGGCTCTCATGCTTTTGCTCGAATGGAAGGAGATATTGACAAGCAAAGAAAACTGATCCTTCAGG
  GACAATTATCAGAGGCAGCTCTGCAGAAGCAGCATCAAAGAAATTATAATCGCTGGTTATGTCAAGCAAG
  ATCTGAGGATCTGTCTGATATTGCTTCTCTAAAAGCCTTATACCAAACAGGTGTTGATAACTGTGGTCGG
  ACAGTGATGGTGGTAGTTGGAAGAAACATTCCTGTAACATTAATAGATATGGACAAGGCTCTCTTATATT
  TCATTCATGTAATGGATCACATTGCTGTGAAGGAGTATGTATTAGTGTATTTTCACACCCTGACCAGCGA
  ATACAATCACCTGGACTCCGACTTCCTGAAGAAACTCTACGATGTTGTTGATGTCAAGTACAAGAGGAAT
  TTGAAGGCTGTTTATTTTGTACATCCCACATTTCGTTCAAAGGTGTCAACATGGTTTTTTACCACCTTTT
  CTGTCTCAGGACTGAAGGACAAAATCCACCATGTGGACAGCCTCCACCAGCTGTTTTCTGCCATATCACC
  AGAACAGATTGACTTTCCTCCTTTTGTCCTTGAATATGATGCCAGGGAAAACGGGCCTTACTATACATCA
  TATCCCCCATCACCAGATTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224299 representing NM_017686
  Red=Cloning site Green=Tags(s)
  
  MDPLGAPSQFVDVDTLPSWGDSCQDELNSSDTTAEIFQEDTVRSPFLYNKDVNGKVVLWKGDVALLNCTA
  IVNTSNESLTDKNPVSESIFMLAGPDLKEDLQKLKGCRTGEAKLTKGFNLAARFIIHTVGPKYKSRYRTA
  AESSLYSCYRNVLQLAKEQSMSSVGFCVINSAKRGYPLEDATHIALRTVRRFLEIHGETIEKVVFAVSDL
  EEGTYQKLLPLYFPRSLKEENRSLPYLPADIGNAEGEPVVPERQIRISEKPGAPEDNQEEEDEGLGVDLS
  FIGSHAFARMEGDIDKQRKLILQGQLSEAALQKQHQRNYNRWLCQARSEDLSDIASLKALYQTGVDNCGR
  TVMVVVGRNIPVTLIDMDKALLYFIHVMDHIAVKEYVLVYFHTLTSEYNHLDSDFLKKLYDVVDVKYKRN
  LKAVYFVHPTFRSKVSTWFFTTFSVSGLKDKIHHVDSLHQLFSAISPEQIDFPPFVLEYDARENGPYYTS
  YPPSPDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017686
• ORF Size: 1491 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017686.2
• RefSeq Size: 2880 bp
• RefSeq ORF: 1494 bp
• Locus ID: 54834
• UniProt ID: Q9NXN4
• Cytogenetics: 1p12
• Domains: SEC14, A1pp
• MW: 56 kDa