THSD4 (NM_024817) Human Tagged ORF Clone

CAT#: RC224213

THSD4 (Myc-DDK-tagged)-Human thrombospondin, type I, domain containing 4 (THSD4)

Product Images

Circular map for RC224213

• 

  Western blot validation of overexpression lysate (Cat# LY411038) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224213 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: THSD4
• Synonyms: ADAMTSL-6; ADAMTSL6; FVSY9334; PRO34005
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224213 representing NM_024817
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTCCCATTTCATGGGGTCTCTCAGTGTCCTGTGTTTCCTTCTGCTGCTTGGATTCCAGTTCGTCT
  GCCCACAGCCCTCCACTCAACACAGGAAGGTCCCGCAGCGGATGGCGGCGGAGGGCGCCCCCGAGGACGA
  CGGCGGCGGCGGCGCCCCGGGAGTGTGGGGCGCCTGGGGCCCCTGGTCGGCCTGCTCGCGTAGCTGCAGC
  GGCGGCGTGATGGAGCAGACGCGGCCCTGCCTGCCCCGCTCCTACCGCCTGCGCGGCGGCCAGCGGCCTG
  GCGCCCCTGCGCGCGCCTTCGCGGACCACGTGGTGTCGGCGGTGCGCACGTCGGTGCCACTGCACCGGAG
  CCGCGACGAGACGCCAGCGCTGGCCGGTACGGACGCCAGCCGCCAGGGCCCCACGGTGCTGCGAGGCAGC
  CGGCACCCACAGCCCCAGGGCCTCGAAGTCACTGGGGACAGAAGGAGCAGGACCCGTGGTACCATTGGCC
  CTGGCAAGTATGGCTATGGTAAGGCCCCATATATCTTACCACTGCAGACAGACACTGCACACACGCCACA
  GAGGCTCCGGAGACAGAAGCTCTCATCCCGCCATTCCAGGTCCCAGGGAGCATCTTCTGCTAGGCATGGC
  TACAGTTCACCAGCCCACCAGGTCCCCCAACATGGGCCTTTGTACCAAAGTGACAGTGGCCCTCGCTCTG
  GACTGCAGGCTGCGGAGGCCCCCATCTACCAGCTACCTTTGACCCATGATCAAGGCTACCCTGCAGCTTC
  AAGTCTCTTTCACAGCCCAGAAACAAGCAACAACCACGGTGTGGGGACCCATGGGGCAACTCAGAGCTTC
  TCTCAGCCTGCCCGATCTACAGCAATCTCATGCATCGGGGCCTATCGGCAGTACAAGCTGTGCAACACCA
  ACGTATGTCCAGAAAGCAGTAGAAGTATCCGGGAGGTACAGTGTGCATCCTACAACAACAAGCCATTCAT
  GGGCCGGTTTTATGAGTGGGAACCATTTGCAGAAGTAAAAGGCAATCGCAAATGTGAGTTGAACTGCCAG
  GCAATGGGCTACCGCTTCTATGTACGGCAAGCTGAGAAAGTCATCGATGGCACCCCCTGTGACCAGAACG
  GCACGGCCATCTGTGTGTCTGGGCAGTGCAAGAGCATTGGCTGTGATGACTACTTAGGCTCCGACAAAGT
  CGTGGACAAATGTGGGGTGTGTGGAGGAGACAACACGGGCTGTCAGGTTGTGTCGGGCGTGTTTAAGCAT
  GCCCTCACCAGCCTGGGCTACCACCGCGTCGTGGAGATTCCCGAGGGAGCCACGAAAATCAACATCACGG
  AGATGTACAAGAGCAACAACTATTTGGCCCTGAGAAGTCGTTCTGGACGCTCCATCATCAATGGGAACTG
  GGCAATTGATCGACCAGGAAAATACGAGGGCGGAGGGACCATGTTCACCTACAAGCGTCCAAATGAGATT
  TCGAGCACTGCCGGAGAGTCCTTTTTGGCGGAAGGTCCCACCAACGAGATCTTGGATGTCTACATGATAC
  ACCAGCAGCCAAACCCAGGCGTGCACTACGAGTACGTGATCATGGGGACCAACGCCATCAGCCCCCAGGT
  GCCACCCCACAGGAGACCAGGGGAACCCTTCAATGGCCAGATGGTGACAGAAGGCAGGAGCCAGGAGGAG
  GGAGAACAGAAAGGGAGGAACGAGGAGAAGGAAGACTTGCGTGGGGAGGCCCCTGAGATGTTCACCTCAG
  AATCGGCACAGACCTTCCCAGTCAGGCATCCAGACAGATTTTCTCCCCATCGACCGGACAACTTGGTGCC
  ACCAGCACCGCAGCCCCCACGGCGCAGCCGGGATCACAACTGGAAGCAGCTTGGGACAACAGAATGTTCC
  ACGACCTGTGGGAAAGGATCGCAGTACCCTATTTTCCGCTGTGTGCACAGAAGCACTCATGAAGAGGCTC
  CTGAGAGTTACTGTGACTCCAGCATGAAGCCGACCCCCGAGGAGGAGCCCTGCAACATCTTCCCTTGCCC
  AGCCTTCTGGGACATCGGGGAGTGGTCTGAGTGCAGCAAGACCTGTGGCCTGGGCATGCAGCACCGCCAG
  GTTCTGTGCCGCCAGGTGTACGCCAACCGCAGCCTGACGGTGCAGCCCTACCGCTGCCAGCACCTGGAGA
  AACCTGAGACCACCAGCACCTGCCAACTCAAGATCTGCAGCGAGTGGCAGATCCGGACCGACTGGACCTC
  GTGCTCGGTGCCCTGCGGCGTGGGACAGAGGACCCGTGATGTGAAGTGTGTGAGCAACATTGGGGATGTG
  GTTGACGATGAGGAATGCAACATGAAGCTCCGGCCGAATGACATTGAGAACTGCGACATGGGACCCTGTG
  CCAAGAGCTGGTTCCTCACCGAGTGGAGCGAAAGGTGCTCAGCGGAGTGTGGGGCCGGAGTGCGGACACG
  CTCGGTGGTGTGCATGACCAACCATGTCAGCAGCCTGCCCCTGGAGGGCTGTGGGAACAACCGGCCGGCA
  GAGGCCACCCCATGTGACAACGGACCCTGCACGGGCAAGGTGGAGTGGTTTGCCGGGAGCTGGAGTCAGT
  GTTCCATCGAGTGTGGGAGCGGGACGCAACAGAGGGAGGTGATTTGTGTTAGAAAGAATGCAGACACCTT
  TGAAGTGTTGGACCCCTCTGAATGTTCTTTCCTGGAGAAACCCCCCAGCCAGCAATCCTGCCACCTCAAG
  CCTTGCGGAGCCAAATGGTTTAGCACCGAATGGAGCATGTGTTCCAAGAGCTGCCAGGGTGGCTTTCGGG
  TCCGGGAAGTGCGGTGTCTGTCTGATGACATGACTCTAAGTAACCTCTGTGACCCTCAGTTGAAACCAGA
  AGAGAGAGAATCTTGTAACCCTCAGGACTGTGTCCCTGAAGTTGATGAAAACTGCAAGGACAAGTACTAC
  AACTGCAACGTGGTGGTCCAGGCAAGACTCTGTGTCTACAACTACTACAAGACCGCCTGCTGTGCCTCCT
  GCACCCGTGTGGCCAACAGGCAGACGGGCTTCCTGGGGAGCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224213 representing NM_024817
  Red=Cloning site Green=Tags(s)
  
  MVSHFMGSLSVLCFLLLLGFQFVCPQPSTQHRKVPQRMAAEGAPEDDGGGGAPGVWGAWGPWSACSRSCS
  GGVMEQTRPCLPRSYRLRGGQRPGAPARAFADHVVSAVRTSVPLHRSRDETPALAGTDASRQGPTVLRGS
  RHPQPQGLEVTGDRRSRTRGTIGPGKYGYGKAPYILPLQTDTAHTPQRLRRQKLSSRHSRSQGASSARHG
  YSSPAHQVPQHGPLYQSDSGPRSGLQAAEAPIYQLPLTHDQGYPAASSLFHSPETSNNHGVGTHGATQSF
  SQPARSTAISCIGAYRQYKLCNTNVCPESSRSIREVQCASYNNKPFMGRFYEWEPFAEVKGNRKCELNCQ
  AMGYRFYVRQAEKVIDGTPCDQNGTAICVSGQCKSIGCDDYLGSDKVVDKCGVCGGDNTGCQVVSGVFKH
  ALTSLGYHRVVEIPEGATKINITEMYKSNNYLALRSRSGRSIINGNWAIDRPGKYEGGGTMFTYKRPNEI
  SSTAGESFLAEGPTNEILDVYMIHQQPNPGVHYEYVIMGTNAISPQVPPHRRPGEPFNGQMVTEGRSQEE
  GEQKGRNEEKEDLRGEAPEMFTSESAQTFPVRHPDRFSPHRPDNLVPPAPQPPRRSRDHNWKQLGTTECS
  TTCGKGSQYPIFRCVHRSTHEEAPESYCDSSMKPTPEEEPCNIFPCPAFWDIGEWSECSKTCGLGMQHRQ
  VLCRQVYANRSLTVQPYRCQHLEKPETTSTCQLKICSEWQIRTDWTSCSVPCGVGQRTRDVKCVSNIGDV
  VDDEECNMKLRPNDIENCDMGPCAKSWFLTEWSERCSAECGAGVRTRSVVCMTNHVSSLPLEGCGNNRPA
  EATPCDNGPCTGKVEWFAGSWSQCSIECGSGTQQREVICVRKNADTFEVLDPSECSFLEKPPSQQSCHLK
  PCGAKWFSTEWSMCSKSCQGGFRVREVRCLSDDMTLSNLCDPQLKPEERESCNPQDCVPEVDENCKDKYY
  NCNVVVQARLCVYNYYKTACCASCTRVANRQTGFLGSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024817
• ORF Size: 3054 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024817.2, NP_079093.2
• RefSeq Size: 9145 bp
• RefSeq ORF: 3057 bp
• Locus ID: 79875
• UniProt ID: Q6ZMP0
• Cytogenetics: 15q23
• Domains: tsp_1
• MW: 112.3 kDa
• Gene Summary: Promotes FBN1 matrix assembly. Attenuates TGFB signaling, possibly by accelerating the sequestration of large latent complexes of TGFB or active TGFB by FBN1 microfibril assembly, thereby negatively regulating the expression of TGFB regulatory targets, such as POSTN (By similarity).[UniProtKB/Swiss-Prot Function]