ANO8 (NM_020959) Human Tagged ORF Clone

CAT#: RC224171

ANO8 (Myc-DDK-tagged)-Human anoctamin 8 (ANO8)

Product Images

Circular map for RC224171

• 

  Western blot validation of overexpression lysate (Cat# LY412182) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224171 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANO8
• Synonyms: KIAA1623; TMEM16H
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224171 representing NM_020959
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGGCCGCCTCCGGCGCCGGGGGCACGTCCCTGGAGGGCGAGCGTGGCAAGAGGCCCCCGCCGG
  AGGGCGAGCCTGCAGCCCCGGCGTCCGGAGTTCTGGATAAGCTTTTCGGAAAGCGGCTCCTGCAGGCTGG
  TCGCTACCTGGTGTCCCACAAGGCGTGGATGAAGACGGTGCCTACAGAGAACTGCGACGTGCTGATGACC
  TTCCCAGACACGACCGATGACCACACGCTGCTATGGCTGCTGAACCACATCCGCGTGGGCATTCCCGAGC
  TCATCGTGCAAGTCCGCCACCACCGCCACACGCGTGCCTACGCCTTCTTTGTCACCGCCACGTATGAGAG
  CCTACTCCGAGGGGCCGACGAGCTGGGTCTGCGCAAAGCAGTGAAGGCCGAGTTTGGCGGGGGCACCCGC
  GGCTTCTCCTGCGAGGAGGACTTTATCTATGAGAATGTGGAGAGCGAGCTACGCTTCTTCACCTCCCAGG
  AACGCCAGAGCATCATCCGCTTCTGGCTGCAGAATTTGCGTGCCAAGCAGGGAGAAGCACTCCACAACGT
  GCGCTTCCTGGAGGACCAGCCAATCATCCCGGAGCTGGCAGCACGTGGGATCATCCAGCAGGTGTTCCCT
  GTCCACGAGCAGCGTATTCTGAACCGCCTCATGAAGTCATGGGTGCAGGCCGTGTGTGAAAACCAGCCTC
  TAGATGACATCTGTGATTACTTTGGTGTGAAAATTGCCATGTACTTCGCCTGGCTGGGCTTCTACACGTC
  GGCTATGGTATACCCAGCTGTCTTCGGGTCTGTCCTGTACACATTCACAGAGGCTGATCAGACAAGCCGG
  GATGTTTCCTGCGTGGTCTTTGCCCTCTTCAACGTGATCTGGTCGACGCTGTTCCTAGAGGAATGGAAGC
  GGAGAGGGGCTGAGCTGGCATATAAGTGGGGGACGCTGGACTCACCTGGGGAAGCCGTGGAGGAGCCACG
  CCCCCAGTTCAGGGGCGTGCGACGTATCAGCCCCATCACGCGGGCCGAGGAGTTCTACTACCCGCCCTGG
  AAGCGGCTGCTCTTCCAGCTGCTTGTGAGCCTCCCCCTGTGCCTGGCGTGCCTCGTCTGTGTCTTCTTGC
  TCATGCTTGGCTGCTTCCAGCTGCAGGAGCTGGTGCTGAGCGTGAAGGGGTTGCCCCGTCTCGCCCGATT
  CCTGCCTAAGGTCATGCTGGCCCTGCTTGTCAGTGTGAGTGCCGAGGGCTACAAGAAGCTAGCCATCTGG
  CTCAATGACATGGAAAATTACCGGCTGGAGAGCGCCTATGAGAAGCACCTCATCATCAAAGTTGTCCTGT
  TCCAGTTTGTCAACTCGTACCTGAGCCTCTTCTACATCGGTTTCTACCTCAAGGACATGGAGCGCTTGAA
  AGAGATGCTGGCCACGCTGCTGATCACCCGCCAGTTCCTCCAGAACGTGCGCGAGGTCCTGCAGCCGCAC
  CTGTACCGGCGCCTGGGCCGCGGCGAGCTGGGCCTGCGGGCCGTCTGGGAGCTGGCCCGAGCCCTGCTTG
  GCCTCCTGAGCCTCCGGCGCCCTGCGCCCCGCCGCCTCGAACCCCAGGCGGATGAGGGCGGGGGCGGCGG
  CAGCGGGGGCGGGGGCCGCAGGTGCCTCAGCGGGGGCTGCGGGGCGCCGGAGGAGGAGGAGGAGGCGGCG
  CTGGTGGAGCGGCGGCGGGCGGGGGAAGGCGGGGAGGAGGGGGACGGGCCTCCAGGGGGCAAGGAGGAGG
  ACGAGGACGACGAGGAGGAGGAGGACGAGGAGGAAGAGGAGGACGAGGAGGAGGGCGAGGAAGGGGGCCT
  CCTGGACTGCGGGCTCCGGCTGAAGAAGGTCAGCTTCGCTGAGCGCGGCGCGGGGCGGCGTCGGCCCGGC
  CCAAGCCCGGAGGCCCTCCTGGAGGAAGGGAGCCCCACTATGGTGGAGAAGGGGCTGGAGCCGGGAGTGT
  TCACCCTGGCCGAGGAGGACGACGAGGCGGAGGGGGCTCCCGGCAGCCCTGAACGGGAGCCCCCGGCCAT
  CTTGTTCCGCCGGGCCGGGGGCGAGGGCCGAGACCAGGGGCCCGACGGGGGCCCGGACCCGGAACCCGGC
  TCCAACAGCGATTCGACCCGTAGGCAGAGACGGCAGAACCGGTCGTCTTGGATTGACCCGCCGGAGGAGG
  AACACTCGCCCCAGCTCACCCAGGCAGAGCTGGAGAGCTGTATGAAGAAGTACGAGGACACGTTCCAGGA
  CTACCAGGAGATGTTCGTGCAGTTCGGCTACGTTGTGCTCTTCTCGTCCGCCTTCCCCCTGGCGGCGCTG
  TGCGCCCTGGTCAACAACCTCATTGAGATCCGCAGCGACGCCTTCAAGCTGTGCACCGGGCTGCAGCGGC
  CCTTCGGCCAGCGCGTGGAAAGCATCGGCCAGTGGCAGAAGGTGATGGAGGCCATGGGTGTCCTAGCGAT
  TGTGGTCAACTGCTACTTAATCGGCCAGTGCGGGCAGCTGCAGCGCCTCTTCCCCTGGCTGAGCCCGGAG
  GCAGCCATCGTGTCGGTGGTAGTGCTCGAGCACTTCGCTCTGCTCCTCAAGTACCTCATCCACGTGGCCA
  TCCCCGATATCCCGGGCTGGGTGGCCGAGGAAATGGCCAAGCTGGAGTACCAGCGCCGCGAGGCCTTTAA
  GAGACACGAGCGCCAGGCCCAGCATCGCTACCAGCAGCAGCAGCGCAGGCGGCGGGAGGAGGAGGAGCGA
  CAGCGCCATGCAGAGCACCATGCCCGGCGGGAGCATGATTCTGGTGGCCGAGAGGAGGCGAGGGCCGAGG
  GCTCTGGGCTGGACCCTGCCACCTCCTCCGAGAAGGCCTCTGCCAAGGCCAAGGGCAGCACTGCGGGTGG
  CCACGGGCCTGAACGGCCCAAGCGCCCAGGGTCCCTGCTGGCACCCAACAACGTCATGAAGTTGAAGCAG
  ATCATCCCACTGCAGGGCAAATTCCTCTCGTCAGGGGCCACATCCTCACTGGCTGCTGCAGGGGCCGGAG
  CCACCACCCGGCCTCCCCCTGCCCAGTCACCCACAGGCAGCGACACCCGCCTGCCTGCCTTCCTCAGCTT
  CAAGTTCCTCAAGTCACCCGAGACCCGGCGGGACTCTGAGCGCAGCCACTCACCGCCCAAAGCCTTCCAT
  GCTGGCAAGCTCTTCCCCTTTGGTGGCACCCGGGCTGAGCCTGGGTCCAACGGGGCGGGCGGGCAGGCCC
  GGCCAGATGGGACCCCCAGCAGTGGCAGCAGCCGGGTTCAGAGGAGTGGGCCGGTGGACGAGGCCCTGGC
  TGAGGAGCTGGAAGCCCCCCGGCCCGAAGAGGAAGGCTCAGGGACAGCGCTGGCCCCCGTGGGCGCCCCT
  GCCCTCCGCACCCGCCGCAGCCGGAGCCCCGCGCCGCCGCCGCCAATGCCGCTGCCCCGGCCCCCGACAC
  CGCCCGCAGGCTGCTGGCAGTGGGACGGGCCCTGGGGCTGCGGGGGCGAGGGTGCCGCCCCCCGCCAGGC
  CCTGGCCGCTGCCGAGTGCCCACCCTGTGCCATGGCCGGGCCCCCACCCGCCCCCCAGCCCCTGCCGGGA
  GACGCCAGCTTTTACAGCCTCCCGCCCCCACCGCTACCGCCCACCTCGGATCCCCTCGAGACCCCAGCGC
  CCTCCCCTAGCCCCAGCCCCAGCCCCCAGGCCGTGTGCTGGCCCAGCGGCTGGCAT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224171 representing NM_020959
  Red=Cloning site Green=Tags(s)
  
  MAEAASGAGGTSLEGERGKRPPPEGEPAAPASGVLDKLFGKRLLQAGRYLVSHKAWMKTVPTENCDVLMT
  FPDTTDDHTLLWLLNHIRVGIPELIVQVRHHRHTRAYAFFVTATYESLLRGADELGLRKAVKAEFGGGTR
  GFSCEEDFIYENVESELRFFTSQERQSIIRFWLQNLRAKQGEALHNVRFLEDQPIIPELAARGIIQQVFP
  VHEQRILNRLMKSWVQAVCENQPLDDICDYFGVKIAMYFAWLGFYTSAMVYPAVFGSVLYTFTEADQTSR
  DVSCVVFALFNVIWSTLFLEEWKRRGAELAYKWGTLDSPGEAVEEPRPQFRGVRRISPITRAEEFYYPPW
  KRLLFQLLVSLPLCLACLVCVFLLMLGCFQLQELVLSVKGLPRLARFLPKVMLALLVSVSAEGYKKLAIW
  LNDMENYRLESAYEKHLIIKVVLFQFVNSYLSLFYIGFYLKDMERLKEMLATLLITRQFLQNVREVLQPH
  LYRRLGRGELGLRAVWELARALLGLLSLRRPAPRRLEPQADEGGGGGSGGGGRRCLSGGCGAPEEEEEAA
  LVERRRAGEGGEEGDGPPGGKEEDEDDEEEEDEEEEEDEEEGEEGGLLDCGLRLKKVSFAERGAGRRRPG
  PSPEALLEEGSPTMVEKGLEPGVFTLAEEDDEAEGAPGSPEREPPAILFRRAGGEGRDQGPDGGPDPEPG
  SNSDSTRRQRRQNRSSWIDPPEEEHSPQLTQAELESCMKKYEDTFQDYQEMFVQFGYVVLFSSAFPLAAL
  CALVNNLIEIRSDAFKLCTGLQRPFGQRVESIGQWQKVMEAMGVLAIVVNCYLIGQCGQLQRLFPWLSPE
  AAIVSVVVLEHFALLLKYLIHVAIPDIPGWVAEEMAKLEYQRREAFKRHERQAQHRYQQQQRRRREEEER
  QRHAEHHARREHDSGGREEARAEGSGLDPATSSEKASAKAKGSTAGGHGPERPKRPGSLLAPNNVMKLKQ
  IIPLQGKFLSSGATSSLAAAGAGATTRPPPAQSPTGSDTRLPAFLSFKFLKSPETRRDSERSHSPPKAFH
  AGKLFPFGGTRAEPGSNGAGGQARPDGTPSSGSSRVQRSGPVDEALAEELEAPRPEEEGSGTALAPVGAP
  ALRTRRSRSPAPPPPMPLPRPPTPPAGCWQWDGPWGCGGEGAAPRQALAAAECPPCAMAGPPPAPQPLPG
  DASFYSLPPPPLPPTSDPLETPAPSPSPSPSPQAVCWPSGWH
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020959
• ORF Size: 3696 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020959.3
• RefSeq Size: 4150 bp
• RefSeq ORF: 3699 bp
• Locus ID: 57719
• UniProt ID: Q9HCE9
• Cytogenetics: 19p13.11
• Protein Families: Transmembrane
• MW: 135.9 kDa
• Gene Summary: Does not exhibit calcium-activated chloride channel (CaCC) activity.[UniProtKB/Swiss-Prot Function]