ZNF510 (NM_014930) Human Tagged ORF Clone

CAT#: RC224010

ZNF510 (Myc-DDK-tagged)-Human zinc finger protein 510 (ZNF510)

Product Images

Circular map for RC224010

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF510
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224010 representing NM_014930
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCCACATCCAGAAGCCATCACAGATTGTGTGACACTGAACACTGTGGGCCAACTTGCAGAAGGTG
  GTTATCCTTTACGGTTCTCCACACTCTTTCAGGAGCAGCAGAAAATGAACATATCTCAGGCATCAGTGTC
  ATTCAAGGACGTGACTATAGAATTCACCCAGGAGGAGTGGCAGCAAATGGCCCCTGTTCAGAAGAATCTG
  TACAGAGATGTGATGCTGGAGAACTACAGCAACCTCGTCTCAGTGGGGTACTGCTGTTTCAAACCAGAGG
  TGATCTTCAAGTTGGAGCAAGGAGAGGAGCCTTGGTTCTCAGAGGAGGAATTCTCAAACCAGAGTCACCC
  AAAAGATTACAGAGGTGATGACCTGATCAAGCAGAACAAGAAAATCAAAGACAAACACTTGGAGCAAGCA
  ATATGTATCAATAATAAAACATTGACTACAGAGGAAGAGAAAGTTTTGGGGAAACCATTTACTCTGCATG
  TAGCTGCTGTTGCTTCAACAAAAATGTCCTGCAAATGCAACTCATGGGAAGTGAATTTGCAAAGTATTTC
  TGAATTTATCATTAATAATAGAAACTATTCAACAAAGAAAATAGGTTGCGGTAATGTATGTGAGAATTCA
  CCTTTCAAAATTAACTTTGAGAAAACTCAGACTGGAGAGAAATTTTATGAACATAATAAAAACATGAAAG
  CTCTCAATTATAATGAAAATCTTCCCAAGCATCCAAAGTTTCAAACTTTGGAGCAAGCTTTTGAATGTAA
  TAAAATTGGAAAAGCCTTTAATGATAAGGCTAACTGTGTTAAACATAACAGTTCTCACACAGGAGAAACA
  TCCTCTAAAGATGATGAATTTAGGAAAAATTGTGATAAGAAAACTCTCTTTGACCACAGGAGAACTGGCA
  CAGGGAAGAAACACCTGCATCTTAATCAATGTGGGAAATCCTTTGAGAAGTCAACTGTGGAGGAATATAA
  TAAACTTAATATGGGTATAAAACATTATGAATTAAATCCAAGTGGAAATAATTTCAACAGAAAGGCACAC
  CTCACTGATCCTCAAACAGCTGTCATAGAAGAGAACCCATTGGTAAGTAATGACAGAACACAGACTTGGG
  TTAAATCCTCTGAATATCATGAAAATAAGAAATCCTACCAGACGTCGGTTCACAGAGTTCGCCGAAGAAG
  TCACTCAATGATGAAACCCTATAAATGTAATGAATGTGGGAAATCCTTCTGTCAGAAAGGACATCTCATT
  CAACATCAGAGAACTCACACAGGAGAGAAACCATTTGAATGTAGTGAATGTGGAAAAACTTTCTCCCAGA
  AGTCACACCTCAGTACTCATCAGAGAATTCATACAGCAGAAAAACCCTATAAATGTAATGAATGTGGAAA
  AACATTTGTCCAGAAGTCAACCCTCAGGGGACATCAAAGAATTCACACAGGAGAAAAACCCTATGAATGT
  AGTGAATGTGGGAAAACTTTTGTTCAGAAGTCCACCCTCAGAGATCATCACAGAATTCACACAGGGGAGA
  AATCCTTTCAATGCAATCAATGTGGAAAAACATTTGGCCAGAAGTCAAACCTCAGAATACATCAGAGAAC
  TCACACTGGGGAGAAAACTTACCAGTGTAATGAATGTGAAAAATCCTTCTGGCGAAAAGATCATCTCATT
  CAACATCAGAAAACTCACACGGGAGAGAAACCATTCAAATGTAACGAATGTGGGAAAACTTTTGCCCGGA
  CATCAACCCTCAGAGTGCATCAAAGAATTCACACTGGGGAGAAACCATTTAAATGTAATGAATGTGGGAA
  GAAATTTGTCCGGAAAGCAATCCTTAGTGATCATCAGAGAATTCACACAGGGGAGAAACCCTTTCAGTGT
  AATAAATGTGGGAAAACTTTTGGCCAGAAATCAAACCTCAGAATACATCAAAGGACTCACAGTGGGGAGA
  AATCTTATGAATGCAATGAATATGGGAAATTATGTAAGAAGTCTACCCTAAGCTTATACCAGAAAATTCA
  GGGAGAGGGGAATCCCTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224010 representing NM_014930
  Red=Cloning site Green=Tags(s)
  
  MSPHPEAITDCVTLNTVGQLAEGGYPLRFSTLFQEQQKMNISQASVSFKDVTIEFTQEEWQQMAPVQKNL
  YRDVMLENYSNLVSVGYCCFKPEVIFKLEQGEEPWFSEEEFSNQSHPKDYRGDDLIKQNKKIKDKHLEQA
  ICINNKTLTTEEEKVLGKPFTLHVAAVASTKMSCKCNSWEVNLQSISEFIINNRNYSTKKIGCGNVCENS
  PFKINFEKTQTGEKFYEHNKNMKALNYNENLPKHPKFQTLEQAFECNKIGKAFNDKANCVKHNSSHTGET
  SSKDDEFRKNCDKKTLFDHRRTGTGKKHLHLNQCGKSFEKSTVEEYNKLNMGIKHYELNPSGNNFNRKAH
  LTDPQTAVIEENPLVSNDRTQTWVKSSEYHENKKSYQTSVHRVRRRSHSMMKPYKCNECGKSFCQKGHLI
  QHQRTHTGEKPFECSECGKTFSQKSHLSTHQRIHTAEKPYKCNECGKTFVQKSTLRGHQRIHTGEKPYEC
  SECGKTFVQKSTLRDHHRIHTGEKSFQCNQCGKTFGQKSNLRIHQRTHTGEKTYQCNECEKSFWRKDHLI
  QHQKTHTGEKPFKCNECGKTFARTSTLRVHQRIHTGEKPFKCNECGKKFVRKAILSDHQRIHTGEKPFQC
  NKCGKTFGQKSNLRIHQRTHSGEKSYECNEYGKLCKKSTLSLYQKIQGEGNPY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014930
• ORF Size: 2049 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014930.2
• RefSeq Size: 5154 bp
• RefSeq ORF: 2052 bp
• Locus ID: 22869
• UniProt ID: Q9Y2H8
• Cytogenetics: 9q22.33
• Domains: KRAB, zf-C2H2
• Protein Families: Transcription Factors
• MW: 79 kDa
• Gene Summary: This gene encodes a krueppel C2H2-type zinc-finger protein family member. The encoded protein is expressed in several cancer cell types and may be a biomarker for early diagnosis of these cancers. [provided by RefSeq, Sep 2015]