PADI2 (NM_007365) Human Tagged ORF Clone

CAT#: RC223889

PADI2 (Myc-DDK-tagged)-Human peptidyl arginine deiminase, type II (PADI2)

Product Images

Western blot validation of overexpression lysate (Cat# LY416014) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223889 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PADI2 protein (Cat# TP323889). The protein was produced from HEK293T cells transfected with PADI2 cDNA clone (Cat# RC223889) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PADI2
• Synonyms: PAD-H19; PAD2; PDI2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223889 representing NM_007365
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGCTGCGCGAGCGGACCGTGCGGCTGCAGTACGGGAGCCGCGTGGAGGCGGTGTACGTGCTGGGCACCT
  ACCTCTGGACCGATGTCTACAGCGCGGCCCCAGCCGGGGCCCAAACCTTCAGCCTGAAGCACTCGGAACA
  CGTGTGGGTGGAGGTGGTGCGTGATGGGGAGGCTGAGGAGGTGGCCACCAATGGCAAGCAGCGCTGGCTT
  CTCTCGCCCAGCACCACCCTGCGGGTCACCATGAGCCAGGCGAGCACCGAGGCCAGCAGTGACAAGGTCA
  CCGTCAACTACTATGACGAGGAAGGGAGCATTCCCATCGACCAGGCGGGGCTCTTCCTCACAGCCATTGA
  GATCTCCCTGGATGTGGACGCAGACCGGGATGGTGTGGTGGAGAAGAACAACCCAAAGAAGGCATCCTGG
  ACCTGGGGCCCCGAGGGCCAGGGGGCCATCCTGCTGGTGAACTGTGACCGAGAGACACCCTGGTTGCCCA
  AGGAGGACTGCCGTGATGAGAAGGTCTACAGCAAGGAAGATCTCAAGGACATGTCCCAGATGATCCTGCG
  GACCAAAGGCCCCGACCGCCTCCCCGCCGGATACGAGATAGTTCTGTACATTTCCATGTCAGACTCAGAC
  AAAGTGGGCGTGTTCTACGTGGAGAACCCGTTCTTCGGCCAACGCTATATCCACATCCTGGGCCGGCGGA
  AGCTCTACCATGTGGTCAAGTACACGGGTGGCTCCGCGGAGCTGCTGTTCTTCGTGGAAGGCCTCTGTTT
  CCCCGACGAGGGCTTCTCAGGCCTGGTCTCCATCCATGTCAGCCTGCTGGAGTACATGGCCCAGGACATT
  CCCCTGACTCCCATCTTCACGGACACCGTGATATTCCGGATTGCTCCGTGGATCATGACCCCCAACATCC
  TGCCTCCCGTGTCGGTGTTTGTGTGCTGCATGAAGGATAATTACCTGTTCCTGAAAGAGGTGAAGAACCT
  TGTGGAGAAAACCAACTGTGAGCTGAAGGTCTGCTTCCAGTACCTAAACCGAGGCGATCGCTGGATCCAG
  GATGAAATTGAGTTTGGCTACATCGAGGCCCCCCATAAAGGCTTCCCCGTGGTGCTGGACTCTCCCCGAG
  ATGGAAACCTAAAGGACTTCCCTGTGAAGGAGCTCCTGGGCCCAGATTTTGGCTACGTGACCCGGGAGCC
  CCTCTTTGAGTCTGTCACCAGCCTTGACTCATTTGGAAACCTGGAGGTCAGTCCCCCAGTGACCGTGAAT
  GGCAAGACATACCCGCTTGGCCGCATCCTCATCGGGAGCAGCTTTCCTCTGTCTGGTGGTCGGAGGATGA
  CCAAGGTGGTGCGTGACTTCCTGAAGGCCCAGCAGGTGCAGGCACCCGTGGAGCTCTACTCAGACTGGCT
  GACTGTGGGCCACGTGGATGAGTTCATGTCCTTTGTCCCCATCCCCGGCACAAAGAAATTCCTGCTACTC
  ATGGCCAGCACCTCGGCCTGCTACAAGCTCTTCCGAGAGAAGCAGAAGGACGGCCATGGAGAGGCCATCA
  TGTTCAAAGGCTTGGGTGGGATGAGCAGCAAGCGAATCACCATCAACAAGATTCTGTCCAACGAGAGCCT
  TGTGCAGGAGAACCTGTACTTCCAGCGCTGCCTAGACTGGAACCGTGACATCCTCAAGAAGGAGCTGGGA
  CTGACAGAGCAGGACATCATTGACCTGCCCGCTCTGTTCAAGATGGACGAGGACCACCGTGCCAGAGCCT
  TCTTCCCAAACATGGTGAACATGATCGTGCTGGACAAGGACCTGGGCATCCCCAAGCCATTCGGGCCACA
  GGTTGAGGAGGAATGCTGCCTGGAGATGCACGTGCGTGGCCTCCTGGAGCCCCTGGGCCTCGAATGCACC
  TTCATCGACGACATTTCTGCCTACCACAAATTTCTGGGGGAAGTCCACTGTGGCACCAACGTCCGCAGGA
  AGCCCTTCACCTTCAAGTGGTGGCACATGGTGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223889 representing NM_007365
  Red=Cloning site Green=Tags(s)
  
  MLRERTVRLQYGSRVEAVYVLGTYLWTDVYSAAPAGAQTFSLKHSEHVWVEVVRDGEAEEVATNGKQRWL
  LSPSTTLRVTMSQASTEASSDKVTVNYYDEEGSIPIDQAGLFLTAIEISLDVDADRDGVVEKNNPKKASW
  TWGPEGQGAILLVNCDRETPWLPKEDCRDEKVYSKEDLKDMSQMILRTKGPDRLPAGYEIVLYISMSDSD
  KVGVFYVENPFFGQRYIHILGRRKLYHVVKYTGGSAELLFFVEGLCFPDEGFSGLVSIHVSLLEYMAQDI
  PLTPIFTDTVIFRIAPWIMTPNILPPVSVFVCCMKDNYLFLKEVKNLVEKTNCELKVCFQYLNRGDRWIQ
  DEIEFGYIEAPHKGFPVVLDSPRDGNLKDFPVKELLGPDFGYVTREPLFESVTSLDSFGNLEVSPPVTVN
  GKTYPLGRILIGSSFPLSGGRRMTKVVRDFLKAQQVQAPVELYSDWLTVGHVDEFMSFVPIPGTKKFLLL
  MASTSACYKLFREKQKDGHGEAIMFKGLGGMSSKRITINKILSNESLVQENLYFQRCLDWNRDILKKELG
  LTEQDIIDLPALFKMDEDHRARAFFPNMVNMIVLDKDLGIPKPFGPQVEEECCLEMHVRGLLEPLGLECT
  FIDDISAYHKFLGEVHCGTNVRRKPFTFKWWHMVP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007365
• ORF Size: 1995 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007365.3
• RefSeq Size: 2348 bp
• RefSeq ORF: 1998 bp
• Locus ID: 11240
• UniProt ID: Q9Y2J8
• Cytogenetics: 1p36.13
• Domains: PAD
• MW: 75.4 kDa
• Gene Summary: This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type II enzyme is the most widely expressed family member. Known substrates for this enzyme include myelin basic protein in the central nervous system and vimentin in skeletal muscle and macrophages. This enzyme is thought to play a role in the onset and progression of neurodegenerative human disorders, including Alzheimer disease and multiple sclerosis, and it has also been implicated in glaucoma pathogenesis. This gene exists in a cluster with four other paralogous genes. [provided by RefSeq, Jul 2008]