CREB5 (NM_004904) Human Tagged ORF Clone

CAT#: RC223869

CREB5 (Myc-DDK-tagged)-Human cAMP responsive element binding protein 5 (CREB5), transcript variant 2

Product Images

Circular map for RC223869

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CREB5
• Synonyms: CRE-BPA; CREB-5; CREBPA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223869 representing NM_004904
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTTGGAGCAGGAGAGGCCGTTTGTCTGCAGTGCCCCAGGCTGCTCCCAGCGCTTCCCAACAGAGG
  ACCATCTGATGATTCATAGGCACAAACATGAAATGACTTTGAAGTTTCCTTCAATAAAAACAGACAATAT
  GTTATCAGATCAAACTCCGACCCCAACGAGATTCCTGAAGAACTGCGAGGAGGTGGGCCTCTTCAGCGAG
  CTGGACTGCTCCCTGGAGCACGAGTTCAGGAAGGCTCAGGAAGAGGAGAGCAGCAAGCGGAATATCTCGA
  TGCATAATGCAGTTGGTGGGGCCATGACGGGGCCCGGAACTCACCAGCTTAGCAGCGCTCGGCTGCCCAA
  CCATGACACCAACGTTGTGATTCAGCAAGCCATGCCGTCGCCTCAGTCCAGCTCTGTCATCACTCAGGCA
  CCTTCCACCAACCGCCAGATCGGGCCTGTCCCAGGCTCTCTATCTTCTCTGCTACATCTCCACAACAGAC
  AGAGACAGCCCATGCCAGCCTCCATGCCTGGGACCCTGCCCAACCCTACAATGCCAGGATCTTCCGCCGT
  CTTGATGCCAATGGAGCGACAAATGTCAGTGAACTCCAGCATCATGGGGATGCAAGGTCCAAATCTCAGC
  AACCCCTGTGCTTCTCCCCAGGTCCAGCCAATGCATTCAGAAGCCAAAATGAGGTTGAAGGCTGCATTGA
  CTCACCACCCTGCTGCCATGTCAAATGGGAACATGAACACCATGGGACACATGATGGAGATGATGGGCTC
  CCGGCAGGACCAGACGCCACACCATCACATGCACTCGCACCCGCATCAGCACCAGACACTGCCACCCCAT
  CACCCTTACCCACACCAGCACCAGCACCCAGCACACCATCCTCACCCTCAACCCCATCACCAGCAGAACC
  ATCCACATCACCACTCCCATTCCCACCTTCATGCACACCCAGCACATCACCAGACCTCGCCACATCCGCC
  CCTGCACACCGGCAACCAAGCACAGGTTTCACCAGCAACACAACAGATGCAGCCAACCCAGACAATACAG
  CCACCCCAGCCCACAGGGGGGCGCCGGCGAAGGGTGGTAGACGAGGATCCGGACGAGAGGCGGCGGAAAT
  TTCTGGAACGGAACCGGGCAGCTGCCACCCGCTGCAGACAGAAGAGGAAGGTCTGGGTGATGTCATTGGA
  AAAGAAAGCAGAAGAACTCACCCAGACAAACATGCAGCTTCAGAATGAAGTGTCTATGTTGAAAAATGAG
  GTGGCCCAGCTGAAACAGTTGTTGTTAACACATAAAGACTGCCCAATAACAGCCATGCAGAAAGAATCAC
  AAGGATATCTAAGTCCAGAGAGTAGCCCTCCTGCTAGTCCTGTCCCAGCTTGCTCCCAGCAACAAGTCAT
  CCAGCATAATACCATCACTACTTCCTCATCGGTCAGCGAGGTGGTAGGAAGCTCCACCCTCAGCCAGCTC
  ACCACTCACAGAACAGACCTGAATCCGATTCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223869 representing NM_004904
  Red=Cloning site Green=Tags(s)
  
  MNLEQERPFVCSAPGCSQRFPTEDHLMIHRHKHEMTLKFPSIKTDNMLSDQTPTPTRFLKNCEEVGLFSE
  LDCSLEHEFRKAQEEESSKRNISMHNAVGGAMTGPGTHQLSSARLPNHDTNVVIQQAMPSPQSSSVITQA
  PSTNRQIGPVPGSLSSLLHLHNRQRQPMPASMPGTLPNPTMPGSSAVLMPMERQMSVNSSIMGMQGPNLS
  NPCASPQVQPMHSEAKMRLKAALTHHPAAMSNGNMNTMGHMMEMMGSRQDQTPHHHMHSHPHQHQTLPPH
  HPYPHQHQHPAHHPHPQPHHQQNHPHHHSHSHLHAHPAHHQTSPHPPLHTGNQAQVSPATQQMQPTQTIQ
  PPQPTGGRRRRVVDEDPDERRRKFLERNRAAATRCRQKRKVWVMSLEKKAEELTQTNMQLQNEVSMLKNE
  VAQLKQLLLTHKDCPITAMQKESQGYLSPESSPPASPVPACSQQQVIQHNTITTSSSVSEVVGSSTLSQL
  TTHRTDLNPIL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004904
• ORF Size: 1503 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004904.3
• RefSeq Size: 8213 bp
• RefSeq ORF: 1506 bp
• Locus ID: 9586
• UniProt ID: Q02930
• Cytogenetics: 7p15.1
• Domains: BRLZ, zf-C2H2
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Prostate cancer
• MW: 55.9 kDa
• Gene Summary: The product of this gene belongs to the CRE (cAMP response element)-binding protein family. Members of this family contain zinc-finger and bZIP DNA-binding domains. The encoded protein specifically binds to CRE as a homodimer or a heterodimer with c-Jun or CRE-BP1, and functions as a CRE-dependent trans-activator. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]