Prickle (PRICKLE1) (NM_153026) Human Tagged ORF Clone

CAT#: RC223861

PRICKLE1 (Myc-DDK-tagged)-Human prickle homolog 1 (Drosophila) (PRICKLE1), transcript variant 1

Product Images

Circular map for RC223861

• 

  Western blot validation of overexpression lysate (Cat# LY403502) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223861 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prickle
• Synonyms: EPM1B; RILP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223861 representing NM_153026
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTTGGAGATGGAGCCCAAGATGAGCAAACTGGCCTTTGGCTGTCAGAGAAGTTCCACATCAGATG
  ATGACTCTGGCTGTGCATTGGAGGAGTACGCCTGGGTCCCCCCGGGCCTGAGACCAGAGCAGATCCAGCT
  CTATTTTGCTTGCTTACCAGAGGAAAAAGTTCCTTACGTTAACAGCCCCGGAGAGAAGCATCGGATTAAA
  CAGCTTTTGTACCAGTTACCACCACATGATAATGAGGTACGGTATTGCCAGTCTTTGAGTGAAGAGGAGA
  AAAAAGAGTTGCAGGTGTTCAGTGCTCAGCGGAAGAAAGAAGCACTGGGAAGAGGAACAATTAAGCTTCT
  GTCCAGAGCAGTCATGCATGCTGTGTGTGAGCAGTGTGGTTTGAAGATAAATGGAGGTGAAGTTGCAGTG
  TTCGCCTCCCGTGCGGGCCCTGGTGTGTGCTGGCACCCATCCTGTTTTGTCTGTTTCACGTGTAATGAGC
  TGCTGGTCGACCTCATCTATTTTTATCAGGATGGAAAAATTCACTGTGGCAGGCACCATGCAGAACTGCT
  CAAACCACGGTGCTCAGCATGTGACGAGATAATTTTTGCTGATGAGTGCACAGAAGCTGAGGGTCGCCAT
  TGGCACATGAAACACTTCTGCTGCCTTGAGTGTGAAACGGTCCTGGGAGGACAGAGGTATATCATGAAGG
  ACGGCCGCCCCTTCTGCTGTGGCTGTTTTGAGTCTCTCTATGCGGAGTACTGTGAAACCTGTGGGGAACA
  TATTGGTGTGGACCATGCACAGATGACCTATGACGGGCAGCACTGGCACGCCACGGAAGCCTGCTTTTCT
  TGTGCCCAGTGTAAAGCCTCTTTGTTGGGATGTCCCTTCCTTCCCAAACAGGGTCAGATTTACTGCTCAA
  AAACGTGCAGTCTTGGTGAAGACGTCCATGCCTCTGATTCTTCCGACTCTGCATTTCAGTCAGCTCGATC
  AAGAGACTCCCGAAGAAGTGTCCGAATGGGCAAGAGCAGCCGGTCAGCAGATCAGTGTAGACAGTCTCTC
  CTCTTATCGCCTGCTCTGAACTACAAGTTTCCTGGCCTCTCAGGCAATGCTGATGACACCCTTTCTCGAA
  AATTGGATGATCTGAGTCTCTCCAGACAAGGAACAAGTTTTGCCAGTGAAGAATTTTGGAAAGGCAGAGT
  AGAGCAGGAAACTCCAGAAGACCCTGAAGAATGGGCTGATCATGAAGATTATATGACGCAGCTCCTCCTC
  AAGTTTGGTGATAAAAGCCTCTTTCAGCCACAGCCCAATGAGATGGATATTCGAGCCAGTGAGCACTGGA
  TATCTGATAACATGGTTAAAAGTAAGACCGAGTTAAAGCAAAATAACCAGAGCCTTGCAAGTAAAAAATA
  CCAGTCTGATATGTACTGGGCACAGTCACAAGATGGACTGGGCGATTCTGCTTATGGCAGCCACCCAGGC
  CCTGCAAGCAGTAGAAGGCTTCAGGAATTGGAACTGGACCATGGGGCTTCAGGGTATAATCATGATGAAA
  CACAGTGGTATGAAGATTCCCTGGAGTGTCTGTCAGACCTGAAACCAGAGCAAAGTGTTCGGGATTCGAT
  GGATTCTTTGGCATTGTCCAATATCACAGGGGCTTCGGTGGATGGAGAAAACAAGCCAAGGCCATCATTG
  TATTCTCTGCAAAATTTTGAGGAGATGGAAACAGAAGATTGTGAGAAGATGAGCAATATGGGAACTTTGA
  ACTCTTCCATGCTGCACAGGAGTGCAGAGTCCTTAAAGAGTCTAAGTTCAGAGTTGTGTCCAGAGAAAAT
  CCTGCCTGAAGAGAAGCCAGTACATCTGCCAGTGCTCAGAAGGTCCAAGTCTCAATCCAGACCCCAGCAG
  GTCAAGTTCTCCGATGATGTCATTGACAATGGGAACTATGACATTGAAATCCGGCAGCCTCCGATGAGTG
  AAAGGACTCGGAGACGCGTCTACAATTTTGAAGAGAGGGGATCCAGGTCTCATCACCACCGCCGCCGGAG
  AAGTAGAAAGTCCCGCTCCGACAATGCCCTGAATCTTGTTACAGAAAGAAAATACTCTCCCAAGGACAGA
  CTGCGGCTGTACACCCCCGATAACTATGAGAAATTTATACAGAATAAAAGTGCCCGGGAGATCCAAGCAT
  ACATCCAGAATGCTGATCTCTACGGACAGTACGCCCATGCCACTTCCGATTATGGCCTGCAGAACCCAGG
  AATGAATCGGTTTCTGGGACTCTACGGCGAGGATGATGATTCCTGGTGTTCTTCCTCCTCCTCCTCTTCC
  GACTCGGAAGAAGAAGGATATTTTCTTGGACAACCAATCCCTCAACCCCGGCCACAGAGATTTGCCTACT
  ATACAGATGACCTTTCTAGTCCACCATCTGCACTTCCCACCCCTCAGTTTGGTCAGAGGACAACAAAATC
  CAAGAAGAAAAAGGGACACAAGGGCAAAAATTGTATTATTTCTAGCGGACCG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223861 representing NM_153026
  Red=Cloning site Green=Tags(s)
  
  MPLEMEPKMSKLAFGCQRSSTSDDDSGCALEEYAWVPPGLRPEQIQLYFACLPEEKVPYVNSPGEKHRIK
  QLLYQLPPHDNEVRYCQSLSEEEKKELQVFSAQRKKEALGRGTIKLLSRAVMHAVCEQCGLKINGGEVAV
  FASRAGPGVCWHPSCFVCFTCNELLVDLIYFYQDGKIHCGRHHAELLKPRCSACDEIIFADECTEAEGRH
  WHMKHFCCLECETVLGGQRYIMKDGRPFCCGCFESLYAEYCETCGEHIGVDHAQMTYDGQHWHATEACFS
  CAQCKASLLGCPFLPKQGQIYCSKTCSLGEDVHASDSSDSAFQSARSRDSRRSVRMGKSSRSADQCRQSL
  LLSPALNYKFPGLSGNADDTLSRKLDDLSLSRQGTSFASEEFWKGRVEQETPEDPEEWADHEDYMTQLLL
  KFGDKSLFQPQPNEMDIRASEHWISDNMVKSKTELKQNNQSLASKKYQSDMYWAQSQDGLGDSAYGSHPG
  PASSRRLQELELDHGASGYNHDETQWYEDSLECLSDLKPEQSVRDSMDSLALSNITGASVDGENKPRPSL
  YSLQNFEEMETEDCEKMSNMGTLNSSMLHRSAESLKSLSSELCPEKILPEEKPVHLPVLRRSKSQSRPQQ
  VKFSDDVIDNGNYDIEIRQPPMSERTRRRVYNFEERGSRSHHHRRRRSRKSRSDNALNLVTERKYSPKDR
  LRLYTPDNYEKFIQNKSAREIQAYIQNADLYGQYAHATSDYGLQNPGMNRFLGLYGEDDDSWCSSSSSSS
  DSEEEGYFLGQPIPQPRPQRFAYYTDDLSSPPSALPTPQFGQRTTKSKKKKGHKGKNCIISSGP
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153026
• ORF Size: 2502 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153026.3
• RefSeq Size: 3293 bp
• RefSeq ORF: 2496 bp
• Locus ID: 144165
• UniProt ID: Q96MT3
• Cytogenetics: 12q12
• Domains: LIM
• Protein Families: Druggable Genome
• Protein Pathways: Wnt signaling pathway
• MW: 94.1 kDa
• Gene Summary: This gene encodes a nuclear receptor that may be a negative regulator of the Wnt/beta-catenin signaling pathway. The encoded protein localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in this gene have been linked to progressive myoclonus epilepsy. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 3. [provided by RefSeq, Sep 2009]