GALNT10 (NM_198321) Human Tagged ORF Clone

CAT#: RC223834

GALNT10 (Myc-DDK-tagged)-Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 10 (GalNAc-T10) (GALNT10)

Product Images

Circular map for RC223834

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GALNT10 (Cat# RC223834, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GALNT10 (Cat# TA809396)(1:2000). Positive lysates LY405019 (100ug) and LC405019 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY405019) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223834 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GALNT10
• Synonyms: GALNACT10; PPGALNACT10; PPGANTASE10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223834 representing NM_198321
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCGGAAGGAGAAGCGGCTCCTGCAGGCGGTGGCGCTGGTGCTGGCGGCCCTGGTCCTCCTGCCCA
  ACGTGGGGCTTTGGGCGCTGTACCGCGAGCGGCAGCCCGACGGCACCCCTGGGGGATCGGGGGCGGCGGT
  GGCGCCGGCGGCGGGACAGGGCTCACACAGTCGACAAAAGAAAACGTTTTTCTTGGGAGATGGGCAGAAG
  CTGAAGGACTGGCATGACAAGGAGGCCATCCGGAGGGACGCTCAGCGCGTAGGAAATGGAGAACAAGGAA
  GACCTTACCCCATGACCGATGCTGAGAGAGTGGATCAGGCATACCGAGAAAATGGATTTAACATCTACGT
  CAGTGATAAAATCTCCTTGAATCGCTCTCTCCCAGATATCCGGCACCCAAACTGCAACAGCAAGCGCTAC
  CTGGAGACACTTCCCAACACAAGCATCATCATCCCCTTCCACAACGAGGGCTGGTCCTCCCTCCTCCGCA
  CCGTCCACAGTGTGCTCAATCGCTCGCCTCCAGAGCTGGTCGCCGAGATTGTACTGGTCGACGACTTCAG
  TGATCGAGAGCACCTGAAGAAGCCTCTTGAAGACTACATGGCCCTTTTCCCCAGTGTGAGGATTCTTCGA
  ACCAAGAAACGGGAAGGGCTGATAAGGACCCGAATGCTGGGGGCCTCAGTGGCAACTGGGGATGTCATCA
  CATTCTTGGATTCACACTGTGAAGCCAATGTCAACTGGCTTCCCCCCTTGCTTGACCGCATTGCTCGGAA
  CCGCAAGACCATTGTGTGCCCGATGATTGATGTAATTGACCATGACGACTTTCGGTACGAGACACAGGCA
  GGGGATGCCATGCGGGGAGCCTTTGACTGGGAGATGTACTACAAGCGGATCCCGATCCCTCCAGAACTGC
  AGAAAGCTGACCCCAGCGACCCATTTGAGTCTCCCGTGATGGCCGGTGGACTGTTCGCCGTGGATCGGAA
  GTGGTTCTGGGAACTCGGCGGGTATGACCCAGGCTTGGAGATCTGGGGAGGGGAGCAGTATGAAATCTCC
  TTCAAGGTGTGGATGTGTGGGGGCCGCATGGAGGACATCCCCTGCTCCAGGGTGGGCCATATCTACAGGA
  AGTATGTGCCCTACAAGGTCCCGGCCGGAGTCAGCCTGGCCCGGAACCTTAAGCGGGTGGCCGAAGTGTG
  GATGGATGAGTACGCAGAGTACATTTACCAGCGCCGGCCTGAATACCGCCACCTCTCCGCTGGGGATGTC
  GCAGTCCAGAAAAAGCTCCGCAGCTCCCTTAACTGCAAGAGTTTCAAGTGGTTTATGACGAAGATAGCCT
  GGGACCTGCCCAAATTCTACCCACCCGTGGAGCCCCCGGCTGCAGCTTGGGGGGAGATCCGAAATGTGGG
  CACAGGGCTGTGTGCAGACACAAAGCACGGGGCCTTGGGCTCCCCACTAAGGCTAGAGGGCTGCGTCCGA
  GGCCGTGGGGAGGCTGCCTGGAACAACATGCAGGTATTCACCTTCACCTGGAGAGAGGACATCCGGCCTG
  GAGACCCCCAGCACACCAAGAAGTTCTGCTTTGATGCCATTTCCCACACCAGCCCTGTCACGCTGTACGA
  CTGCCACAGCATGAAGGGCAACCAGCTGTGGAAATACCGCAAAGACAAGACCCTGTACCACCCTGTCAGT
  GGCAGCTGCATGGACTGCAGTGAAAGTGACCATAGGATCTTCATGAACACCTGCAACCCATCCTCTCTCA
  CCCAGCAGTGGCTGTTTGAACACACCAACTCAACAGTCTTGGAAAAATTCAATAGGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223834 representing NM_198321
  Red=Cloning site Green=Tags(s)
  
  MRRKEKRLLQAVALVLAALVLLPNVGLWALYRERQPDGTPGGSGAAVAPAAGQGSHSRQKKTFFLGDGQK
  LKDWHDKEAIRRDAQRVGNGEQGRPYPMTDAERVDQAYRENGFNIYVSDKISLNRSLPDIRHPNCNSKRY
  LETLPNTSIIIPFHNEGWSSLLRTVHSVLNRSPPELVAEIVLVDDFSDREHLKKPLEDYMALFPSVRILR
  TKKREGLIRTRMLGASVATGDVITFLDSHCEANVNWLPPLLDRIARNRKTIVCPMIDVIDHDDFRYETQA
  GDAMRGAFDWEMYYKRIPIPPELQKADPSDPFESPVMAGGLFAVDRKWFWELGGYDPGLEIWGGEQYEIS
  FKVWMCGGRMEDIPCSRVGHIYRKYVPYKVPAGVSLARNLKRVAEVWMDEYAEYIYQRRPEYRHLSAGDV
  AVQKKLRSSLNCKSFKWFMTKIAWDLPKFYPPVEPPAAAWGEIRNVGTGLCADTKHGALGSPLRLEGCVR
  GRGEAAWNNMQVFTFTWREDIRPGDPQHTKKFCFDAISHTSPVTLYDCHSMKGNQLWKYRKDKTLYHPVS
  GSCMDCSESDHRIFMNTCNPSSLTQQWLFEHTNSTVLEKFNRN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198321
• ORF Size: 1809 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198321.4
• RefSeq Size: 5222 bp
• RefSeq ORF: 1812 bp
• Locus ID: 55568
• UniProt ID: Q86SR1
• Cytogenetics: 5q33.2
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis
• MW: 68.8 kDa
• Gene Summary: This gene encodes a member of the GalNAc polypeptide N-acetylgalactosaminyltransferases. These enzymes catalyze the first step in the synthesis of mucin-type oligosaccharides. These proteins transfer GalNAc from UDP-GalNAc to either serine or threonine residues of polypeptide acceptors. The protein encoded by this locus may have increased catalytic activity toward glycosylated peptides compared to activity toward non-glycosylated peptides.[provided by RefSeq, Apr 2010]