TAS2R43 (NM_176884) Human Tagged ORF Clone
CAT#: RC223792
- TrueORF®
TAS2R43 (Myc-DDK-tagged)-Human taste receptor, type 2, member 43 (TAS2R43)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_176884" in other vectors (4)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | TAS2R43 |
Synonyms | T2R43; T2R52 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC223792 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATAACTTTTCTACCCATCATTTTTTCCAGTCTGGTAGTGGTTACATTTGTTATTGGAAATTTTGCTA ATGGCTTCATAGCACTGGTAAATTCCATTGAGTCGTTCAAGAGACAAAAGATCTCCTTTGCTGACCAAAT TCTCACTGCTCTGGCGGTCTCCAGAGTTGGTTTGCTCTGGGTATTATTATTAAACTGGTATTCAACTGTG TTGAATCCAGCTTTTAATAGTGTAGAAGTAAGAACTACTGCTTATAATATCTGGGCAGTGATCAACCATT TCAGCAACTGGCTTGCTACTACCCTCAGCATATTTTATTTGCTCAAGATTGCCAATTTCTCCAACTTTAT TTTTCTTCACTTAAAGAGGAGAGTTAAGAGTGTCATTCTGGTGATGTTGTTGGGGCCTTTGCTATTTTTG GCTTGTCATCTTTTTGTGATAAACATGAATGAGATTGTGCGGACAAAAGAATTTGAAGGAAACATGACTT GGAAGATCAAATTGAAGAGTGCAATGTACTTTTCAAATATGACTGTAACCATGGTAGCAAACTTAGTACC CTTCACTCTGACCCTACTATCTTTTATGCTGTTAATCTGTTCTTTGTGTAAACATCTCAAGAAGATGCAG CTCCGTGGTAAAGGATCTCAAGATCCCAGCACGAAGGTCCACATAAAAGCTTTGCAAACTGTGATCTCCT TCCTCTTGTTATGTGCCATTTACTTTCTGTCCATAATGATATCAGTTTGGAGTTTTGGAAGTCTGGAAAA CAAACCTGTCTTCATGTTCTGCAAAGCTATTAGATTCAGCTATCCTTCAATCCACCCATTCATCCTGATT TGGGGAAACAAGAAGCTAAAGCAGACTTTTCTTTCAGTTTTTTGGCAAATGAGGTACTGGGTGAAAGGAG AGAAGACTTCATCTCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223792 protein sequence
Red=Cloning site Green=Tags(s) MITFLPIIFSSLVVVTFVIGNFANGFIALVNSIESFKRQKISFADQILTALAVSRVGLLWVLLLNWYSTV LNPAFNSVEVRTTAYNIWAVINHFSNWLATTLSIFYLLKIANFSNFIFLHLKRRVKSVILVMLLGPLLFL ACHLFVINMNEIVRTKEFEGNMTWKIKLKSAMYFSNMTVTMVANLVPFTLTLLSFMLLICSLCKHLKKMQ LRGKGSQDPSTKVHIKALQTVISFLLLCAIYFLSIMISVWSFGSLENKPVFMFCKAIRFSYPSIHPFILI WGNKKLKQTFLSVFWQMRYWVKGEKTSSP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_176884 |
ORF Size | 927 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_176884.1, NP_795365.1 |
RefSeq Size | 1027 bp |
RefSeq ORF | 930 bp |
Locus ID | 259289 |
UniProt ID | P59537 |
Cytogenetics | 12p13.2 |
Protein Pathways | Taste transduction |
MW | 35.5 kDa |
Gene Summary | TAS2R43 belongs to the large TAS2R receptor family. TAS2Rs are expressed on the surface of taste receptor cells and mediate the perception of bitterness through a G protein-coupled second messenger pathway (Conte et al., 2002 [PubMed 12584440]). For further information on TAS2Rs, see MIM 604791.[supplied by OMIM, Mar 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC223792L3 | Lenti ORF clone of Human taste receptor, type 2, member 43 (TAS2R43), Myc-DDK-tagged |
USD 600.00 |
|
RC223792L4 | Lenti ORF clone of Human taste receptor, type 2, member 43 (TAS2R43), mGFP tagged |
USD 600.00 |
|
RG223792 | TAS2R43 (tGFP-tagged) - Human taste receptor, type 2, member 43 (TAS2R43) |
USD 500.00 |
|
SC106975 | TAS2R43 (untagged)-Human taste receptor, type 2, member 43 (TAS2R43) |
USD 300.00 |
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