OSBP2 (NM_030758) Human Tagged ORF Clone

CAT#: RC223738

OSBP2 (Myc-DDK-tagged)-Human oxysterol binding protein 2 (OSBP2), transcript variant 1

Product Images

Circular map for RC223738

• 

  Western blot validation of overexpression lysate (Cat# LY410730) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223738 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified OSBP2 protein (Cat# TP323738). The protein was produced from HEK293T cells transfected with OSBP2 cDNA clone (Cat# RC223738) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OSBP2
• Synonyms: HLM; ORP-4; ORP4; OSBPL1; OSBPL4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223738 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAAAGCGGCGGCTCCGAGCCGAGGCGGCGGCTGTGGCGGCCGCTCCCGCGGGCTCTCGTCGCTGT
  TCACGGTTGTCCCCTGCCTGTCGTGCCACACGGCGGCGCCGGGCATGAGCGCTTCCACGTCCGGCTCCGG
  GCCGGAGCCCAAGCCCCAGCCCCAGCCCGTGCCCGAACCGGAGCGGGGACCGCTGTCAGAACAGGTGTCG
  GAGGCAGTTTCGGAGGCAGTGCCAAGATCGGAACCTGTGTCCGAGACGACGTCTGAGCCGGAGCCAGGGG
  CTGGGCAGCCATCGGAACTGCTGCAGGGGTCGCGGCCGGGGTCAGAGTCAAGCTCAGGTGTAGGGGCTGG
  GCCCTTCACTAAGGCCGCATCGGAGCCGCTCTCCCGGGCGGTGGGGAGCGCGACCTTTCTCAGACCCGAG
  TCAGGATCGCTGCCAGCGTTAAAGCCCCTGCCTCTTCTGCGACCAGGACAGGCGAAGACTCCTCTTGGGG
  TTCCAATGTCGGGGACTGGCACGACCTCCAGTGCCCCACTGGCCTTACTGCCTCTGGACAGCTTCGAGGG
  CTGGCTTCTCAAGTGGACCAACTATCTGAAGGGCTACCAGCGCCGCTGGTTCGTGCTGGGCAATGGTTTG
  CTCTCTTACTACAGAAATCAGGGTGAAATGGCCCACACGTGCCGTGGAACCATCAACCTGTCCACCGCGC
  ACATTGACACGGAGGACTCTTGTGGTATCTTGCTGACCAGTGGGGCCAGGAGCTACCACCTCAAGGCCAG
  CTCAGAGGTGGACCGGCAGCAGTGGATCACCGCCCTGGAGCTGGCCAAGGCCAAGGCTGTCCGCGTGATG
  AACACTCATTCAGATGACTCTGGGGACGACGACGAGGCTACCACCCCAGCCGACAAGAGCGAGCTGCACC
  ACACCCTGAAGAATCTTTCCCTGAAGTTAGATGACCTCAGCACGTGCAATGACCTCATCGCCAAGCACGG
  CGCCGCACTCCAGCGCTCCCTGACAGAGCTGGACGGCCTCAAGATCCCATCTGAGAGTGGGGAGAAGCTG
  AAGGTGGTGAATGAGCGGGCCACCCTCTTCCGCATCACATCCAATGCTATGATCAACGCCTGCAGGGACT
  TCTTGGAACTAGCAGAGATACACAGTCGGAAATGGCAGCGGGCACTGCAGTATGAGCAGGAGCAGCGCGT
  GCACTTGGAGGAAACCATTGAGCAGCTGGCGAAGCAGCACAACAGCCTCGAGCGGGCCTTCCACAGTGCC
  CCTGGCCGGCCGGCCAACCCCTCCAAGAGCTTCATTGAGGGAAGCCTCTTGACTCCCAAAGGAGAGGACA
  GTGAGGAAGATGAAGATACCGAGTACTTTGATGCCATGGAAGACTCCACATCCTTCATCACCGTGATCAC
  CGAGGCCAAGGAAGACAGAAAAGCTGAAGGTAGCACCGGGACAAGTTCCGTGGACTGGAGCTCAGCAGAC
  AATGTACTAGATGGTGCCTCGCTCGTGCCCAAGGGTTCATCCAAAGTCAAGAGGCGAGTCCGCATTCCCA
  ACAAGCCCAACTACAGCCTTAACCTCTGGAGCATCATGAAGAACTGCATCGGCCGGGAGCTCTCCAGGAT
  CCCCATGCCGGTGAACTTCAATGAGCCCCTGTCCATGCTCCAGCGGCTGACAGAGGACCTGGAGTACCAC
  CACCTGCTGGACAAGGCAGTGCACTGCACCAGCTCAGTGGAGCAGATGTGCCTGGTGGCCGCCTTCTCTG
  TGTCCTCCTACTCCACCACAGTGCACCGCATCGCCAAGCCCTTCAACCCCATGCTGGGGGAGACCTTCGA
  GCTGGACCGCCTCGACGACATGGGCCTGCGCTCCCTCTGTGAGCAGGTGAGCCACCACCCCCCCTCAGCT
  GCGCACTACGTGTTCTCCAAGCATGGCTGGAGCCTCTGGCAGGAGATCACCATCTCCAGCAAGTTCCGGG
  GAAAATACATCTCCATCATGCCGCTAGGTGCCATCCACTTAGAATTCCAGGCCAGTGGGAATCACTACGT
  GTGGAGGAAGAGCACCTCAACTGTTCACAACATCATCGTGGGCAAGCTCTGGATCGACCAGTCAGGGGAC
  ATCGAGATTGTGAACCATAAGACCAATGACCGGTGCCAGCTGAAGTTCCTGCCCTACAGCTACTTCTCCA
  AAGAGGCAGCCCGGAAGGTGACAGGAGTGGTGAGTGACAGCCAGGGCAAGGCCCATTACGTGCTGTCCGG
  CTCGTGGGATGAACAAATGGAGTGCTCCAAGGTCATGCATAGCAGTCCCAGCAGCCCCAGCTCTGACGGG
  AAGCAGAAGACAGTGTACCAGACCCTGTCAGCCAAGCTGCTGTGGAAGAAGTACCCGCTGCCGGAGAACG
  CGGAGAACATGTACTACTTCTCAGAGCTGGCCCTGACCCTCAACGAGCACGAGGAGGGCGTAGCGCCAAC
  CGACAGCCGCCTGCGGCCCGACCAGCGGCTGATGGAGAAGGGCCGTTGGGACGAGGCCAATACCGAGAAG
  CAGCGGCTGGAGGAGAAGCAGCGCCTGTCGCGGCGCCGGCGGCTGGAGGCCTGCGGGCCGGGCAGCAGCT
  GCAGCTCGGAGGAAGAGAAGGAGGCGGATGCCTACACGCCACTGTGGTTTGAGAAGAGGCTGGATCCGCT
  GACCGGGGAGATGGCCTGTGTGTACAAGGGCGGCTACTGGGAGGCCAAGGAGAAGCAAGACTGGCATATG
  TGCCCCAACATCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223738 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGKAAAPSRGGGCGGRSRGLSSLFTVVPCLSCHTAAPGMSASTSGSGPEPKPQPQPVPEPERGPLSEQVS
  EAVSEAVPRSEPVSETTSEPEPGAGQPSELLQGSRPGSESSSGVGAGPFTKAASEPLSRAVGSATFLRPE
  SGSLPALKPLPLLRPGQAKTPLGVPMSGTGTTSSAPLALLPLDSFEGWLLKWTNYLKGYQRRWFVLGNGL
  LSYYRNQGEMAHTCRGTINLSTAHIDTEDSCGILLTSGARSYHLKASSEVDRQQWITALELAKAKAVRVM
  NTHSDDSGDDDEATTPADKSELHHTLKNLSLKLDDLSTCNDLIAKHGAALQRSLTELDGLKIPSESGEKL
  KVVNERATLFRITSNAMINACRDFLELAEIHSRKWQRALQYEQEQRVHLEETIEQLAKQHNSLERAFHSA
  PGRPANPSKSFIEGSLLTPKGEDSEEDEDTEYFDAMEDSTSFITVITEAKEDRKAEGSTGTSSVDWSSAD
  NVLDGASLVPKGSSKVKRRVRIPNKPNYSLNLWSIMKNCIGRELSRIPMPVNFNEPLSMLQRLTEDLEYH
  HLLDKAVHCTSSVEQMCLVAAFSVSSYSTTVHRIAKPFNPMLGETFELDRLDDMGLRSLCEQVSHHPPSA
  AHYVFSKHGWSLWQEITISSKFRGKYISIMPLGAIHLEFQASGNHYVWRKSTSTVHNIIVGKLWIDQSGD
  IEIVNHKTNDRCQLKFLPYSYFSKEAARKVTGVVSDSQGKAHYVLSGSWDEQMECSKVMHSSPSSPSSDG
  KQKTVYQTLSAKLLWKKYPLPENAENMYYFSELALTLNEHEEGVAPTDSRLRPDQRLMEKGRWDEANTEK
  QRLEEKQRLSRRRRLEACGPGSSCSSEEEKEADAYTPLWFEKRLDPLTGEMACVYKGGYWEAKEKQDWHM
  CPNIF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030758
• ORF Size: 2745 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030758.4
• RefSeq Size: 4349 bp
• RefSeq ORF: 2751 bp
• Locus ID: 23762
• UniProt ID: Q969R2
• Cytogenetics: 22q12.2
• Domains: Oxysterol_BP, PH
• MW: 101.2 kDa
• Gene Summary: The protein encoded by this gene contains a pleckstrin homology (PH) domain and an oxysterol-binding region. It binds oxysterols such as 7-ketocholesterol and may inhibit their cytotoxicity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2013]