Olfactory Receptor OR2A4 (OR2A4) (NM_030908) Human Tagged ORF Clone

CAT#: RC223604

OR2A4 (Myc-DDK-tagged)-Human olfactory receptor, family 2, subfamily A, member 4 (OR2A4)

Product Images

Circular map for RC223604

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  Western blot validation of overexpression lysate (Cat# LY410667) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223604 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Olfactory Receptor OR2A4
• Synonyms: OR2A10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223604 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGACAATATAACATCCATCAGAGAGTTCCTCCTACTGGGATTTCCCGTTGGCCCAAGGATTCAGA
  TGCTCCTCTTTGGGCTCTTCTCCCTGTTCTACGTCTTCACCCTGCTGGGGAACGGGACCATACTGGGGCT
  CATCTCACTGGACTCCAGACTGCACGCCCCCATGTACTTCTTCCTCTCACACCTGGCGGTCGTCGACATC
  GCCTACGCCTGCAACACGGTGCCCCGGATGCTGGTGAACCTCCTGCATCCAGCCAAGCCCATCTCCTTTG
  CGGGCCGCATGATGCAGACCTTTCTGTTTTCCACTTTTGCTGTCACAGAATGTCTCCTCCTGGTGGTGAT
  GTCCTATGATCTGTACGTGGCCATCTGCCACCCCCTCCGATATTTGGCCATCATGACCTGGAGAGTCTGC
  ATCACCCTCGCGGTGACTTCCTGGACCACTGGAGTCCTTTTATCCTTGATTCATCTTGTGTTACTTCTAC
  CTTTACCCTTCTGTAGGCCCCAGAAAATTTATCACTTTTTTTGTGAAATCTTGGCTGTTCTCAAACTTGC
  CTGTGCAGATACCCACATCAATGAGAACATGGTCTTGGCCGGAGCAATTTCTGGGCTGGTGGGACCCTTG
  TCCACAATTGTAGTTTCATATATGTGCATCCTCTGTGCTATCCTTCAGATCCAATCAAGGGAAGTTCAGA
  GGAAAGCCTTCCGCACCTGCTTCTCCCACCTCTGTGTGATTGGACTCGTTTATGGCACAGCCATTATCAT
  GTATGTTGGACCCAGATATGGGAACCCCAAGGAGCAGAAGAAATATCTCCTGCTGTTTCACAGCCTCTTT
  AATCCCATGCTCAATCCCCTTATCTGTAGTCTTAGGAACTCAGAAGTGAAGAATACTTTGAAGAGAGTGC
  TGGGAGTAGAAAGGGCTTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223604 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGDNITSIREFLLLGFPVGPRIQMLLFGLFSLFYVFTLLGNGTILGLISLDSRLHAPMYFFLSHLAVVDI
  AYACNTVPRMLVNLLHPAKPISFAGRMMQTFLFSTFAVTECLLLVVMSYDLYVAICHPLRYLAIMTWRVC
  ITLAVTSWTTGVLLSLIHLVLLLPLPFCRPQKIYHFFCEILAVLKLACADTHINENMVLAGAISGLVGPL
  STIVVSYMCILCAILQIQSREVQRKAFRTCFSHLCVIGLVYGTAIIMYVGPRYGNPKEQKKYLLLFHSLF
  NPMLNPLICSLRNSEVKNTLKRVLGVERAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030908
• ORF Size: 930 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030908.2
• RefSeq Size: 1758 bp
• RefSeq ORF: 933 bp
• Locus ID: 79541
• UniProt ID: O95047
• Cytogenetics: 6q23.2
• Domains: 7tm_1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Olfactory transduction
• MW: 34.8 kDa
• Gene Summary: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]