Nogo A (RTN4) (NM_020532) Human Tagged ORF Clone

CAT#: RC223470

RTN4 (Myc-DDK-tagged)-Human reticulon 4 (RTN4), transcript variant 1

Product Images

Circular map for RC223470

• 

  Western blot validation of overexpression lysate (Cat# LY412411) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223470 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nogo A
• Synonyms: ASY; Nbla00271; Nbla10545; NI220/250; NOGO; NSP; NSP-CL; RTN-X; RTN4-A; RTN4-B1; RTN4-B2; RTN4-C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223470 representing NM_020532
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGACCTGGACCAGTCTCCTCTGGTCTCGTCCTCGGACAGCCCACCCCGGCCGCAGCCCGCGTTCA
  AGTACCAGTTCGTGAGGGAGCCCGAGGACGAGGAGGAAGAAGAGGAGGAGGAAGAGGAGGACGAGGACGA
  AGACCTGGAGGAGCTGGAGGTGCTGGAGAGGAAGCCCGCCGCCGGGCTGTCCGCGGCCCCAGTGCCCACC
  GCCCCTGCCGCCGGCGCGCCCCTGATGGACTTCGGAAATGACTTCGTGCCGCCGGCGCCCCGGGGACCCC
  TGCCGGCCGCTCCCCCCGTCGCCCCGGAGCGGCAGCCGTCTTGGGACCCGAGCCCGGTGTCGTCGACCGT
  GCCCGCGCCATCCCCGCTGTCTGCTGCCGCAGTCTCGCCCTCCAAGCTCCCTGAGGACGACGAGCCTCCG
  GCCCGGCCTCCCCCTCCTCCCCCGGCCAGCGTGAGCCCCCAGGCAGAGCCCGTGTGGACCCCGCCAGCCC
  CGGCTCCCGCCGCGCCCCCCTCCACCCCGGCCGCGCCCAAGCGCAGGGGCTCCTCGGGCTCAGTGGATGA
  GACCCTTTTTGCTCTTCCTGCTGCATCTGAGCCTGTGATACGCTCCTCTGCAGAAAATATGGACTTGAAG
  GAGCAGCCAGGTAACACTATTTCGGCTGGTCAAGAGGATTTCCCATCTGTCCTGCTTGAAACTGCTGCTT
  CTCTTCCTTCTCTGTCTCCTCTCTCAGCCGCTTCTTTCAAAGAACATGAATACCTTGGTAATTTGTCAAC
  AGTATTACCCACTGAAGGAACACTTCAAGAAAATGTCAGTGAAGCTTCTAAAGAGGTCTCAGAGAAGGCA
  AAAACTCTACTCATAGATAGAGATTTAACAGAGTTTTCAGAATTAGAATACTCAGAAATGGGATCATCGT
  TCAGTGTCTCTCCAAAAGCAGAATCTGCCGTAATAGTAGCAAATCCTAGGGAAGAAATAATCGTGAAAAA
  TAAAGATGAAGAAGAGAAGTTAGTTAGTAATAACATCCTTCATAATCAACAAGAGTTACCTACAGCTCTT
  ACTAAATTGGTTAAAGAGGATGAAGTTGTGTCTTCAGAAAAAGCAAAAGACAGTTTTAATGAAAAGAGAG
  TTGCAGTGGAAGCTCCTATGAGGGAGGAATATGCAGACTTCAAACCATTTGAGCGAGTATGGGAAGTGAA
  AGATAGTAAGGAAGATAGTGATATGTTGGCTGCTGGAGGTAAAATCGAGAGCAACTTGGAAAGTAAAGTG
  GATAAAAAATGTTTTGCAGATAGCCTTGAGCAAACTAATCACGAAAAAGATAGTGAGAGTAGTAATGATG
  ATACTTCTTTCCCCAGTACGCCAGAAGGTATAAAGGATCGTTCAGGAGCATATATCACATGTGCTCCCTT
  TAACCCAGCAGCAACTGAGAGCATTGCAACAAACATTTTTCCTTTGTTAGGAGATCCTACTTCAGAAAAT
  AAGACCGATGAAAAAAAAATAGAAGAAAAGAAGGCCCAAATAGTAACAGAGAAGAATACTAGCACCAAAA
  CATCAAACCCTTTTCTTGTAGCAGCACAGGATTCTGAGACAGATTATGTCACAACAGATAATTTAACAAA
  GGTGACTGAGGAAGTCGTGGCAAACATGCCTGAAGGCCTGACTCCAGATTTAGTACAGGAAGCATGTGAA
  AGTGAATTGAATGAAGTTACTGGTACAAAGATTGCTTATGAAACAAAAATGGACTTGGTTCAAACATCAG
  AAGTTATGCAAGAGTCACTCTATCCTGCAGCACAGCTTTGCCCATCATTTGAAGAGTCAGAAGCTACTCC
  TTCACCAGTTTTGCCTGACATTGTTATGGAAGCACCATTGAATTCTGCAGTTCCTAGTGCTGGTGCTTCC
  GTGATACAGCCCAGCTCATCACCATTAGAAGCTTCTTCAGTTAATTATGAAAGCATAAAACATGAGCCTG
  AAAACCCCCCACCATATGAAGAGGCCATGAGTGTATCACTAAAAAAAGTATCAGGAATAAAGGAAGAAAT
  TAAAGAGCCTGAAAATATTAATGCAGCTCTTCAAGAAACAGAAGCTCCTTATATATCTATTGCATGTGAT
  TTAATTAAAGAAACAAAGCTTTCTGCTGAACCAGCTCCGGATTTCTCTGATTATTCAGAAATGGCAAAAG
  TTGAACAGCCAGTGCCTGATCATTCTGAGCTAGTTGAAGATTCCTCACCTGATTCTGAACCAGTTGACTT
  ATTTAGTGATGATTCAATACCTGACGTTCCACAAAAACAAGATGAAACTGTGATGCTTGTGAAAGAAAGT
  CTCACTGAGACTTCATTTGAGTCAATGATAGAATATGAAAATAAGGAAAAACTCAGTGCTTTGCCACCTG
  AGGGAGGAAAGCCATATTTGGAATCTTTTAAGCTCAGTTTAGATAACACAAAAGATACCCTGTTACCTGA
  TGAAGTTTCAACATTGAGCAAAAAGGAGAAAATTCCTTTGCAGATGGAGGAGCTCAGTACTGCAGTTTAT
  TCAAATGATGACTTATTTATTTCTAAGGAAGCACAGATAAGAGAAACTGAAACGTTTTCAGATTCATCTC
  CAATTGAAATTATAGATGAGTTCCCTACATTGATCAGTTCTAAAACTGATTCATTTTCTAAATTAGCCAG
  GGAATATACTGACCTAGAAGTATCCCACAAAAGTGAAATTGCTAATGCCCCGGATGGAGCTGGGTCATTG
  CCTTGCACAGAATTGCCCCATGACCTTTCTTTGAAGAACATACAACCCAAAGTTGAAGAGAAAATCAGTT
  TCTCAGATGACTTTTCTAAAAATGGGTCTGCTACATCAAAGGTGCTCTTATTGCCTCCAGATGTTTCTGC
  TTTGGCCACTCAAGCAGAGATAGAGAGCATAGTTAAACCCAAAGTTCTTGTGAAAGAAGCTGAGAAAAAA
  CTTCCTTCCGATACAGAAAAAGAGGACAGATCACCATCTGCTATATTTTCAGCAGAGCTGAGTAAAACTT
  CAGTTGTTGACCTCCTGTACTGGAGAGACATTAAGAAGACTGGAGTGGTGTTTGGTGCCAGCCTATTCCT
  GCTGCTTTCATTGACAGTATTCAGCATTGTGAGCGTAACAGCCTACATTGCCTTGGCCCTGCTCTCTGTG
  ACCATCAGCTTTAGGATATACAAGGGTGTGATCCAAGCTATCCAGAAATCAGATGAAGGCCACCCATTCA
  GGGCATATCTGGAATCTGAAGTTGCTATATCTGAGGAGTTGGTTCAGAAGTACAGTAATTCTGCTCTTGG
  TCATGTGAACTGCACGATAAAGGAACTCAGGCGCCTCTTCTTAGTTGATGATTTAGTTGATTCTCTGAAG
  TTTGCAGTGTTGATGTGGGTATTTACCTATGTTGGTGCCTTGTTTAATGGTCTGACACTACTGATTTTGG
  CTCTCATTTCACTCTTCAGTGTTCCTGTTATTTATGAACGGCATCAGGCACAGATAGATCATTATCTAGG
  ACTTGCAAATAAGAATGTTAAAGATGCTATGGCTAAAATCCAAGCAAAAATCCCTGGATTGAAGCGCAAA
  GCTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223470 representing NM_020532
  Red=Cloning site Green=Tags(s)
  
  MEDLDQSPLVSSSDSPPRPQPAFKYQFVREPEDEEEEEEEEEEDEDEDLEELEVLERKPAAGLSAAPVPT
  APAAGAPLMDFGNDFVPPAPRGPLPAAPPVAPERQPSWDPSPVSSTVPAPSPLSAAAVSPSKLPEDDEPP
  ARPPPPPPASVSPQAEPVWTPPAPAPAAPPSTPAAPKRRGSSGSVDETLFALPAASEPVIRSSAENMDLK
  EQPGNTISAGQEDFPSVLLETAASLPSLSPLSAASFKEHEYLGNLSTVLPTEGTLQENVSEASKEVSEKA
  KTLLIDRDLTEFSELEYSEMGSSFSVSPKAESAVIVANPREEIIVKNKDEEEKLVSNNILHNQQELPTAL
  TKLVKEDEVVSSEKAKDSFNEKRVAVEAPMREEYADFKPFERVWEVKDSKEDSDMLAAGGKIESNLESKV
  DKKCFADSLEQTNHEKDSESSNDDTSFPSTPEGIKDRSGAYITCAPFNPAATESIATNIFPLLGDPTSEN
  KTDEKKIEEKKAQIVTEKNTSTKTSNPFLVAAQDSETDYVTTDNLTKVTEEVVANMPEGLTPDLVQEACE
  SELNEVTGTKIAYETKMDLVQTSEVMQESLYPAAQLCPSFEESEATPSPVLPDIVMEAPLNSAVPSAGAS
  VIQPSSSPLEASSVNYESIKHEPENPPPYEEAMSVSLKKVSGIKEEIKEPENINAALQETEAPYISIACD
  LIKETKLSAEPAPDFSDYSEMAKVEQPVPDHSELVEDSSPDSEPVDLFSDDSIPDVPQKQDETVMLVKES
  LTETSFESMIEYENKEKLSALPPEGGKPYLESFKLSLDNTKDTLLPDEVSTLSKKEKIPLQMEELSTAVY
  SNDDLFISKEAQIRETETFSDSSPIEIIDEFPTLISSKTDSFSKLAREYTDLEVSHKSEIANAPDGAGSL
  PCTELPHDLSLKNIQPKVEEKISFSDDFSKNGSATSKVLLLPPDVSALATQAEIESIVKPKVLVKEAEKK
  LPSDTEKEDRSPSAIFSAELSKTSVVDLLYWRDIKKTGVVFGASLFLLLSLTVFSIVSVTAYIALALLSV
  TISFRIYKGVIQAIQKSDEGHPFRAYLESEVAISEELVQKYSNSALGHVNCTIKELRRLFLVDDLVDSLK
  FAVLMWVFTYVGALFNGLTLLILALISLFSVPVIYERHQAQIDHYLGLANKNVKDAMAKIQAKIPGLKRK
  AE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020532
• ORF Size: 3576 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020532.5
• RefSeq Size: 4871 bp
• RefSeq ORF: 3579 bp
• Locus ID: 57142
• UniProt ID: Q9NQC3
• Cytogenetics: 2p16.1
• Domains: Reticulon
• Protein Families: Transmembrane
• MW: 129.8 kDa
• Gene Summary: This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]