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KIF13A (NM_022113) Human Tagged ORF Clone
CAT#: RC223328
KIF13A (Myc-DDK-tagged)-Human kinesin family member 13A (KIF13A), transcript variant 1
ORF Plasmid: tGFP
"NM_022113" in other vectors (2)
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Frequently bought together (5)
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | KIF13A |
| Synonyms | bA500C11.2; RBKIN |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223328 representing NM_022113
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGATACCAAGGTAAAAGTTGCCGTCCGGGTCCGGCCCATGAACCGACGAGAACTGGAACTGAACA CCAAGTGCGTGGTGGAGATGGAAGGGAATCAAACGGTCCTGCACCCTCCTCCTTCTAACACCAAACAGGG AGAAAGGAAACCTCCCAAGGTATTTGCCTTTGATTATTGCTTTTGGTCCATGGATGAATCTAACACTACA AAATACGCTGGTCAAGAAGTGGTTTTCAAGTGCCTTGGGGAAGGAATTCTTGAAAAAGCCTTTCAGGGGT ATAATGCGTGTATTTTTGCATATGGACAGACAGGTTCGGGAAAATCCTTTTCCATGATGGGCCATGCTGA GCAGCTGGGCCTTATTCCAAGGCTCTGCTGTGCTTTATTTAAAAGGATCTCTTTGGAGCAAAATGAGTCA CAGACCTTTAAAGTTGAAGTGTCCTATATGGAAATTTATAATGAGAAAGTTCGGGATCTTTTAGACCCCA AAGGGAGTAGACAGTCTCTTAAAGTTCGAGAACATAAAGTTTTGGGACCATATGTAGATGGTTTATCTCA ACTAGCTGTCACTAGTTTTGAGGATATTGAGTCATTGATGTCTGAGGGAAATAAGTCTCGAACGGTAGCT GCTACCAACATGAACGAAGAAAGCAGCCGCTCCCATGCTGTGTTCAACATCATAATCACACAGACACTTT ATGACCTGCAGTCTGGGAATTCCGGGGAGAAAGTCAGTAAGGTCAGCTTGGTAGACCTGGCGGGTAGCGA AAGAGTATCTAAAACAGGAGCTGCAGGAGAGCGACTGAAAGAAGGCAGCAACATTAACAAATCGCTTACA ACCTTGGGGTTGGTTATATCATCACTGGCTGACCAGGCAGCTGGCAAGGGTAAAAGCAAATTTGTGCCTT ATCGAGATTCAGTCCTCACTTGGCTGCTTAAGGACAACTTGGGGGGCAACAGCCAAACCTCTATGATAGC CACAATCAGCCCAGCCGCAGACAACTATGAAGAGACCCTCTCCACATTAAGATATGCAGACCGAGCCAAA AGGATTGTGAACCATGCTGTTGTGAATGAGGACCCCAACGCAAAAGTGATCCGAGAACTGCGGGAGGAAG TCGAGAAACTGAGAGAGCAGCTCTCTCAGGCAGAGGCCATGAAGGCCCCTGAACTGAAGGAGAAGCTCGA AGAGTCTGAAAAGCTGATAAAAGAACTAACAGTGACTTGGGAAGAGAAGCTGAGAAAAACAGAAGAGATA GCACAGGAAAGACAACGACAACTTGAAAGCATGGGGATTTCCCTGGAGATGTCCGGTATCAAGGTGGGGG ATGACAAATGCTACTTAGTCAATCTGAATGCAGACCCTGCTCTTAACGAACTTCTGGTTTATTATTTAAA GGATCACACCAGGGTGGGTGCAGATACCTCTCAAGATATCCAGCTTTTTGGCATAGGAATTCAGCCTCAG CACTGTGAGATTGACATTGCATCTGATGGAGACGTCACTCTCACTCCAAAAGAAAATGCAAGGTCCTGTG TGAACGGCACCCTTGTGTGCAGTACCACCCAGCTGTGGCATGGTGACCGAATCCTATGGGGAAATAATCA CTTTTTTAGAATAAACTTACCTAAGAGGAAACGTCGAGATTGGTTGAAAGACTTTGAAAAAGAAACGGGC CCGCCAGAGCATGACCTGGATGCAGCCAGTGAGGCTTCCTCTGAACCAGACTATAACTATGAATTTGCAC AGATGGAAGTTATCATGAAAACCCTGAATAGTAATGACCCAGTTCAAAATGTGGTTCAGGTCCTGGAGAA ACAATACCTAGAAGAAAAGAGAAGTGCCCTAGAGGAGCAGCGGCTCATGTATGAGCGGGAACTGGAGCAA CTCCGCCAGCAGCTCTCCCCCGACAGGCAGCCACAGAGTAGCGGCCCTGACCGCCTGGCCTACAGCAGCC AGACAGCGCAGCAGAAGGTGACCCAGTGGGCAGAAGAGAGGGATGAACTCTTCCGACAAGGCCTGGCAAA ACTGCGAGAGCAGCTGGTTAAAGCTAATACCTTGGTGAGGGAAGCAAACTTCCTGGCTGAGGAAATGAGC AAACTCACCGATTACCAAGTGACTCTTCAGATCCCTGCTGCAAACCTCAGTGCCAATAGGAAGAGAGGTG CAATAGTGAGTGAACCAGCTATCCAAGTGAGGAGGAAAGGAAAGAGCACCCAAGTGTGGACCATTGAGAA GCTGGAGAATAAATTAATTGACATGAGAGACCTTTACCAAGAATGGAAGGAAAAAGTTCCTGAGGCAAAG AGACTCTACGGAAAACGAGGTGACCCTTTCTATGAAGCTCAAGAAAATCACAACCTCATCGGGGTGGCGA ATGTATTCTTGGAATGCCTCTTCTGTGATGTGAAACTTCAGTATGCAGTCCCTATCATCAGCCAGCAGGG GGAGGTTGCAGGGCGTCTCCACGTGGAAGTGATGCGTGTTACAGGAGCTGTTCCAGAGCGTGTGGTGGAG GATGACTCTTCGGAGAATTCCAGTGAAAGTGGGAGCCTTGAAGTCGTAGACAGCAGCGGGGAAATCATTC ACCGAGTCAAAAAGCTGACATGTCGGGTAAAAATTAAAGAAGCAACGGGGCTGCCCTTAAACCTCTCAAA TTTTGTCTTCTGTCAATACACATTCTGGGACCAGTGTGAGTCTACGGTGGCTGCCCCGGTGGTGGACCCC GAGGTGCCTTCACCACAGTCCAAGGATGCCCAGTACACAGTGGCCTTCTCCCACTGTAAGGACTATGTGG TGAATGTAACAGAAGAATTTCTGGAGTTCATTTCAGATGGAGCACTGGCCATTGAAGTATGGGGCCACCG GTGTGCTGGAAATGGCAGCTCCATCTGGGAGGTCGATTCTCTTCATGCTAAGACAAGAACACTGCATGAC AGGTGGAATGAAGTAACGCGAAGAATAGAAATGTGGATCTCCATATTAGAATTGAATGAGTTAGGAGAGT ATGCTGCAGTGGAACTTCATCAGGCAAAAGATGTCAACACAGGAGGCATCTTTCAACTTAGACAGGGTCA TTCCCGTAGAGTACAAGTCACGGTGAAACCTGTGCAGCATTCAGGGACACTGCCACTTATGGTTGAAGCC ATCCTGTCAGTATCCATCGGCTGTGTAACTGCCAGGTCCACCAAACTCCAAAGAGGGCTGGACAGTTACC AGAGAGATGATGAGGATGGTGATGATATGGATAGTTATCAGGAAGAAGACTTAAACTGCGTAAGGGAGAG GTGGTCAGATGCACTCATTAAACGACGAGAATACCTGGATGAACAGATAAAAAAAGTCAGCAATAAAACA GAGAAAACAGAGGACGATGTGGAGCGGGAAGCCCAGCTTGTGGAGCAGTGGGTAGGGCTGACTGAGGAAA GGAATGCTGTGCTGGTGCCAGCCCCAGGCAGTGGGATTCCTGGGGCACCTGCCGACTGGATCCCACCTCC TGGAATGGAAACCCACATACCAGTTCTCTTCCTCGATTTGAATGCGGATGACCCCAGTGCCAATGAGCAG CTTGTTGGCCCCCATGCATCCGGCGTGAACTCCATCCTGCCCAAGGAGCATGGCAGTCAGTTTTTCTACC TGCCCATCATAAAGCACAGTGATGATGAGGTTTCAGCCACAGCCTCTTGGGATTCCTCGGTGCATGATTC TGTTCACTTGAATAGGGTCACACCACAGAATGAAAGGATTTACCTAATTGTGAAAACCACAGTTCAACTC AGCCACCCTGCTGCTATGGAGTTAGTATTACGAAAACGAATTGCAGCCAATATTTACAACAAACAGAGTT TCACGCAGAGTTTGAAGAGGAGAATATCCCTGAAAAATATATTTTATTCCTGTGGTGTAACCTATGAAAT AGTATCCAATATACCAAAGGCAACTGAGGAGATAGAGGACCGGGAAACGCTGGCTCTCCTGGCAGCAAGG AGTGAAAACGAAGGCACATCAGATGGGGAGACGTACATTGAGAAGTACACTCGAGGCGTGCTGCAGGTGG AAAACATTCTGAGTCTTGAACGGCTCCGGCAGGCCGTCACAGTCAAAGAAGCACTTTCCACCAAAGCCCG GCACATTCGGAGGAGCCTCAGTACACCAAATGTTCATAATGTCTCTTCCAGCCGACCGGACCTTTCTGGC TTTGATGAAGATGACAAGGGTTGGCCAGAGAACCAGTTGGACATGTCTGACTATAGCTCCAGTTACCAAG ATGTAGCATGTTATGGAACTTTACCCAGGGATTCTCCTCGAAGGAATAAAGAAGGTTGTACATCAGAGAC TCCTCATGCCTTAACCGTCAGCCCTTTTAAAGCATTCTCTCCTCAGCCACCAAAGTTTTTCAAGCCCCTA ATGCCTGTAAAAGAGGAGCATAAGAAAAGGATAGCCCTGGAAGCAAGGCCTCTTCTAAGCCAGGAGAGCA TGCCTCCACCTCAGGCACATAACCCTGGCTGCATTGTACCCTCAGGAAGCAATGGCAGCAGCATGCCAGT AGAACACAATAACAAACGTGAGAAGAAGATTGACTCTGAGGAGGAAGAAAATGAGCTGGAAGCTATTAAC AGGAAGCTAATAAGTTCACAGCCTTATGTACCTGTGGAGTTTGCTGACTTCAGTGTTTACAATGCCAGCT TGGAGAACAGGGAATGGTTTTCCTCTAAAGTAGATCTGTCAAACTCACGGGTCTTGGAGAAAGAAGTGTC CCGTAGCCCTACCACCAGCAGTATTACCAGTGGCTACTTTTCCCACAGTGCCTCCAATGCCACCCTGTCT GACATGGTGGTCCCTTCTAGTGACAGCTCAGACCAGCTGGCCATTCAGACGAAGGATGCAGACTCCACCG AGCACTCCACACCATCGCTTGTGCATGATTTCAGGCCGTCCTCAAACAAAGAGTTGACAGAAGTCGAAAA AGGCTTGGTAAAGGACAAGATAATTGTGGTGCCACTCAAGGAAAACAGTGCCTTAGCCAAAGGGAGCCCA TCATCCCAGAGCATCCCTGAGAAAAACTCCAAATCACTGTGCAGGACTGGCTCATGTTCAGAACTAGATG CCTGCCCCAGCAAAATTAGCCAGCCAGCCAGGGGATTCTGCCCCAGGGAGGTGACGGTAGAACACACCAC CAACATCCTTGAAGACCATTCTTTCACAGAATTTATGGGAGTGTCAGAGGGAAAAGATTTTGATGGTTTG ACAGATTCTTCTGCTGGAGAGCTTTCCAGTAGGAGGAGTCTACCAAATAAAACAGGCGGCAAGACTGTCT CCGATGGGCTCCACCACCCCAGCCAGCTGCATTCCAAGTTAGAGAATGACCAGGTAATAATTCCAGAGGC AGCCTTTTGGGTTCTGTGCTGTCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223328 representing NM_022113
Red=Cloning site Green=Tags(s) MSDTKVKVAVRVRPMNRRELELNTKCVVEMEGNQTVLHPPPSNTKQGERKPPKVFAFDYCFWSMDESNTT KYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGHAEQLGLIPRLCCALFKRISLEQNES QTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEGNKSRTVA ATNMNEESSRSHAVFNIIITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLT TLGLVISSLADQAAGKGKSKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTLRYADRAK RIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEKLRKTEEI AQERQRQLESMGISLEMSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLFGIGIQPQ HCEIDIASDGDVTLTPKENARSCVNGTLVCSTTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETG PPEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNDPVQNVVQVLEKQYLEEKRSALEEQRLMYERELEQ LRQQLSPDRQPQSSGPDRLAYSSQTAQQKVTQWAEERDELFRQGLAKLREQLVKANTLVREANFLAEEMS KLTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKGKSTQVWTIEKLENKLIDMRDLYQEWKEKVPEAK RLYGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGAVPERVVE DDSSENSSESGSLEVVDSSGEIIHRVKKLTCRVKIKEATGLPLNLSNFVFCQYTFWDQCESTVAAPVVDP EVPSPQSKDAQYTVAFSHCKDYVVNVTEEFLEFISDGALAIEVWGHRCAGNGSSIWEVDSLHAKTRTLHD RWNEVTRRIEMWISILELNELGEYAAVELHQAKDVNTGGIFQLRQGHSRRVQVTVKPVQHSGTLPLMVEA ILSVSIGCVTARSTKLQRGLDSYQRDDEDGDDMDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKT EKTEDDVEREAQLVEQWVGLTEERNAVLVPAPGSGIPGAPADWIPPPGMETHIPVLFLDLNADDPSANEQ LVGPHASGVNSILPKEHGSQFFYLPIIKHSDDEVSATASWDSSVHDSVHLNRVTPQNERIYLIVKTTVQL SHPAAMELVLRKRIAANIYNKQSFTQSLKRRISLKNIFYSCGVTYEIVSNIPKATEEIEDRETLALLAAR SENEGTSDGETYIEKYTRGVLQVENILSLERLRQAVTVKEALSTKARHIRRSLSTPNVHNVSSSRPDLSG FDEDDKGWPENQLDMSDYSSSYQDVACYGTLPRDSPRRNKEGCTSETPHALTVSPFKAFSPQPPKFFKPL MPVKEEHKKRIALEARPLLSQESMPPPQAHNPGCIVPSGSNGSSMPVEHNNKREKKIDSEEEENELEAIN RKLISSQPYVPVEFADFSVYNASLENREWFSSKVDLSNSRVLEKEVSRSPTTSSITSGYFSHSASNATLS DMVVPSSDSSDQLAIQTKDADSTEHSTPSLVHDFRPSSNKELTEVEKGLVKDKIIVVPLKENSALAKGSP SSQSIPEKNSKSLCRTGSCSELDACPSKISQPARGFCPREVTVEHTTNILEDHSFTEFMGVSEGKDFDGL TDSSAGELSSRRSLPNKTGGKTVSDGLHHPSQLHSKLENDQVIIPEAAFWVLCCQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022113 |
| ORF Size | 5415 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022113.3, NP_071396.3 |
| RefSeq Size | 5878 bp |
| RefSeq ORF | 5418 bp |
| Locus ID | 63971 |
| UniProt ID | Q9H1H9 |
| Cytogenetics | 6p22.3 |
| Domains | kinesin |
| Protein Families | Druggable Genome |
| MW | 202.1 kDa |
| Gene Summary | This gene encodes a member of the kinesin family of microtubule-based motor proteins that function in the positioning of endosomes. This family member can direct mannose-6-phosphate receptor-containing vesicles from the trans-Golgi network to the plasma membrane, and it is necessary for the steady-state distribution of late endosomes/lysosomes. It is also required for the translocation of FYVE-CENT and TTC19 from the centrosome to the midbody during cytokinesis, and it plays a role in melanosome maturation. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2011] |
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