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KIAA0100 (NM_014680) Human Tagged ORF Clone
CAT#: RC223287
- TrueORF®
KIAA0100 (Myc-DDK-tagged)-Human KIAA0100 (KIAA0100)
ORF Plasmid: tGFP
"NM_014680" in other vectors (2)
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Frequently bought together (5)
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
USD 198.00
Other products for "KIAA0100"
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | KIAA0100 |
| Synonyms | BCOX; BCOX1; CT101; FMP27 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223287 representing NM_014680
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCTGTTCTTCTCCGCGCTGTTGGTCTTGCTGCTAGTTGCGCTTAGCGCCCTCTTTCTAGGCCGGT GGCTTGTGGTCCGGTTGGCCACCAAGTGGTGTCAGCGGAAGCTGCAGGCGGAGCTAAAGATTGGCTCCTT CCGCTTTTTTTGGATCCAGAATGTCAGTCTTAAGTTTCAGCAACACCAGCAAACAGTGGAAATTGATAAC CTGTGGATTTCCAGCAAACTCCTTAGCCATGATCTTCCACACTATGTGGCATTGTGCTTTGGAGAAGTGC GTATCAGAACGGACCTACAGAAAGTTTCTGACCTGTCTGCCCCATTCTCCCAGAGCGCTGGGGTGGATCA AAAGGAACTGTCCTTCAGCCCATCCTTATTGAAGATCTTCTGCCAACTATTCTCCATTCATGTAGATGCT ATAAACATCATGGTTCTCAAGGTGGATACCTCTGAGTCCTTATGGCATATTCAGATCAGTAGAAGCAGAT TTCTTTTGGATAGTGATGGGAAAAGGCTAATCTGTGAGGTGAGCTTATGTAAGATCAACAGCAAAGTTCT AAAGAGTGGTCAGCTGGAGGACACCTGCCTAGTGGAGCTTTCACTGGCCCTGGACCTGTGTCTAAAGGTG GGCATTAGCAGTCGGCATCTCACTGCTATCACTGTGGATGTGTGGACACTCCATGCTGAACTGCATGAGG GCCTCTTCCAGAGCCAACTGCTGTGCCAGGGCCCAAGCCTAGCATCTAAGCCTGTTCCCTGTTCAGAGGT GACAGAAAACTTAGTTGAGCCAACTCTGCCTGGCCTATTCCTTCTCCAGCAGCTGCCAGACCAGGTCAAG GTTAAGATGGAGAACACAAGCGTGGTATTGTCCATGAATAGTCAAAAGAGGCACCTGACTTGGACTCTGA AGCTGCTGCAGTTCCTGTACCACCGTGATGAGGATCAGCTGCCCCTTCGAAGCTTCACAGCAAACTCTGA TATGGCACAGATGAGCACTGAACTGCTGCTGGAAGATGGGTTGTTGTTGTCCCAGAGTCGCCAACGCATT GTCTGCCTCAACTCCCTCAAGGCTAGTGTGCAGGTGACCACCATTGACCTCTCAGCCTCCCTAGTTCTGA ACACTTGCATCATTCACTACCGGCACCAGGAATTCTCTCACTGGCTGCACCTGCTAGCACTGGAAACCCA AGGGTCTAGTTCACCTGTTCTAAAGCAAAGGAAAAAAAGAACCTTCCCCCAAATCCTGGCTCCCATCATC TTTAGCACCTCCATCTCCAATGTCAACATTTCCATTCAACTTGGAGATACACCACCTTTTGCCTTGGGAT TCAATTCTATCTCTCTGGATTACCAGCACCTCAGGCCACAAAGCATCCATCAGCGGGGCGTCCTAACTGT GGACCACCTCTGCTGGCGTGTGGGCAGTGACTCCCACATTCAGCGGGCGCCACACCCACCCAATATGCAT GTTTGGGGTGAGGCACTTGTTCTGGACTCCTTCACACTACAGGGTAGCTATAACCAGCCTCTGGGCCTGT CCAGCACCCAGTCAGATACCCTTTTTCTTGATTGTACCATTCGAGGACTTCAGGTGGAAGCATCAGATAC CTGTGCCCAATGTCTGTCTCGTATCTTATCCCTGATGGGTCCACAATCTGGGAAGTCAGCTGTCTCTAGG CACTCTTCATTTGGGGAATCTGTGTCATTACTGTGGAAGGTGGACTTGAAGGTCGAAGACATGAACTTGT TTACCCTTTCTGCCTTGGTTGGTGCTTCAGAGGTACGACTGGACACCCTAACTATCCTGGGCAGTGCAGA GACGTCCACTGTGGGGATTCAAGGACTTGTGTTAGCGCTGGTGAAATCAGTCACGGAGAAGATGCAACCC TGTTGCAAGGCCCCTGACATCCCTACCCCAGTGCTCAGCCTTTCCATGCTCTCCATCACCTATCACAGCA GCATCCGCTCTCTGGAGGTTCAGTGTGGTGCAGGGCTGACCTTACTTTGGAGCCCCCCAGATCACATGTA CCTGTACCAGCATGTCCTGGCCACTCTACAGTGCCGAGACCTACTAAGAGCCACTGTGTTTCCTGAGACT GTACCATCCCTTGCACTAGAGACTTCAGGAACTACTTCTGAGCTAGAAGGCCGTGCCCCTGAGCCATTAC CCCCAAAGCGGCTGCTAAACCTAACCCTGGAGGTGAGCACAGCCAAGCTCACAGCTTTTGTAGCTGAGGA CAAGTTCATTACCCTGGCTGCAGAGAGTGTGTCACTGAGCCGGCATGGAGGTTCCCTGCAGGCATACTGT CCAGAGCTGGCTGCTGGCTTTGATGGCAATAGTATCTTCAACTTCAAGGAGGTGGAGGTGCAGCTGCTAC CTGAGCTGGAAGAGATGATCCTCCACCGGAACCCCTTCCCTGCGCTGCAGACCCTCCGGAACCGTGTTTG GCTCCTCTCTTTCGGCTCAGTCTCGGTGGAGTTTCCTTATCAGTATGACTTTTCTCGAACTCTAGATGAG GCTGTGGGAGTTCAGAAGTGGCTGAAGGGACTACATCAAGGGACTCGTGCTTGGGCCTCTCCAAGCCCTG TCCCACTCCCACCTGATCTACTCTTAAAGGTTGAGCACTTCTCATGGGTTTTCTTGGATGATGTTTTTGA GGTGAAACTTCATGATAACTACGAGCTGATGAAGGATGAAAGTAAGGAGAGTGCCAAAAGACTACAGCTA CTGGATGCTAAAGTGGCCGCCCTTCGGAAGCAGCATGGGGAGTTGTTGCCTGCCCGCAAAATTGAGGAGC TCTATGCCTCTTTGGAACGCAAAAACATTGAAATCTACATCCAGCGTTCCCGTCGTCTCTATGGCAACAC ACCCATGCGCCGGGCACTGCTTACTTGGAGCTTAGCAGGGCTAGAACTGGTAGCTCTGGCAGATGCCTCC TTCCATGGTCCTGAGCATGTGGTAGAACAGGTTCAAGAGCTTGATCCAGGCAGCCCTTTTCCCCCTGAGG GATTAGATCTTGTCATTCAGTGGTGTCGAATGCTCAAGTGCAATGTCAAGAGCTTTCTGGTTCGGATCAG GGACTATCCACGGTACCTGTTTGAGATCCGTGACTGGCGGCTAATGGGTCGACTTGTGGGCACCGAGCAG AGTGGTCAGCCTTGCTCCCGTCGGCGTCAGATCTTGCACTTGGGGCTTCCGTGGGGTAACGTGGCAGTGG AGAGGAACATGCCCCCACTCAAATTCTACCATGACTTTCACTCGGAAATATTCCAGTACACAGTGGTGTG GGGCCCATGCTGGGATCCAGCCTGGACACTAATTGGCCAGTGTGTGGACCTCTTGACCAAGCCCTCAGCT GACCCCAGCCCACCTTTGCCCTGGTGGGACAAGAGCCGTCTTCTGTTCCATGGAGACTGGCACATGGACA TTGAACAGGCGAACCTGCACCAGCTGGCCACTGAGGATCCATACAACACAACTGAAAATATGCACTGGGA GTGGAGCCACCTGTCTTTTCATTGGAAACCTGGTCAGTTTGTGTTCAAGGGTGACTTGGATATCAACGTG AGAACAGCCTCTAAGTATGACGACTGCTGCTTCCTTCACCTGCCTGACCTCTGCATGACACTGGACCTGC AGTGGCTGTGCCATGGGAACCCCCATGATCACCATAGTGTCACTCTGCGGGCCCCAGAGTTCCTGCCTGA GGTGCCCTTGGGCCAGCTTCATGACTCCTACCGGGCCTTTCGCTCGGAGAACCTCAATCTCTCCATCAAG ATGGATCTGACTCGGCACAGTGGAACAATATCCCAGCCCCGAATTCTGCTATATAGTAGTACCCTGCGCT GGATGCAAAACTTCTGGGCAACTTGGACAAGTGTCACAAGGCCTATCTGCAGGGGAAAGCTCTTCAATAA CCTGAAACCCAGCAAGAAGAAACTTGGTCAGCACTACAAGCAACTTTCCTATACAGCCCTCTTTCCCCAG CTGCAGGTACATTATTGGGCCTCATTTGCCCAGCAACGGGGCATCCAGATTGAGTGCAGTCAGGGCCATG TCTTCACTCGGGGGACTCAGCGGCTTATACCTCAAGCAGGCACAGTGATGCGGCGCCTTATCTCTGATTG GAGTGTTACCCAGATGGTGAGTGACCTAAGTCAGGTGACCGTTCACCTGATGGCCTCACCCACTGAAGAG AATGCTGATCACTGTCTTGATCCCTTGGTAACAAAGACCCACCTGCTGAGCTTGTCCTCCCTCACCTACC AACGGCATAGCAATCGCACAGCTGAGGAGGAGCTCTCTGCTCGTGATGGGGATCCTACCTTTCATACACA TCAGCTGCACTTAGTAGATTTACGGATTTCCTGGACAACTACCAATCGAGACATTGCCTTTGGGTTATAT GATGGGTACAAAAAGGCAGCTGTACTCAAACGTAATCTTTCTACTGAGGCCCTGAAGGGGTTAAAGATTG ATCCACAGATGCCAGCCAAAAAGCCAAAGCGGGGTGTCCCAACTAGTGCCTCAGCCCCACCTCGTGTTAA CACTCCCAGCTTCAGTGGACAACCTGATAAGGGGTCATCAGGAGGTGCTTACATGTTGCAGAAGCTAATT GAAGAGACAGATAGGTTTGTAGTGTTCACAGAAGAGGAATCAGGCATGAGTGACCAGTTGTGTGGCATTG CTGCCTGCCAGACGGATGACATATACAACCGAAACTGCCTTATTGAATTGGTCAACTGTCAGATGGTTCT TCGTGGAGCAGAGACAGAAGGCTGTGTCATTGTGTCAGCTGCCAAAGCCCAACTGCTGCAGTGCCAGCAC CATCCAGCCTGGTATGGTGATACATTGAAGCAAAAGACATCCTGGACTTGCCTCTTGGATGGCATGCAGT ACTTTGCCACCACTGAAAGCAGCCCCACAGAGCAGGATGGCCGACAGCTCTGGTTAGAGGTGAAGAATAT CGAGGAGCACCGGCAGCGTAGTCTGGACTCTGTGCAGGAGCTGATGGAGAGTGGGCAGGCAGTGGGCGGC ATGGTTACCACAACCACAGATTGGAACCAGCCAGCTGAGGCACAGCAAGCCCAGCAAGTCCAGCGGATCA TTTCGCGTTGCAACTGCCGAATGTACTATATTAGTTACAGCCATGACATTGATCCTGAACTAGCAACTCA GATTAAGCCACCTGAAGTTCTTGAGAACCAGGAAAAGGAAGATCTCCTAAAGAAGCAGGAAGGGGCTGTG GATACCTTCACCCTTATCCACCATGAGCTGGAAATTTCCACCAACCCAGCTCAGTATGCCATGATCCTGG ACATTGTCAACAACCTGCTGCTCCATGTAGAACCTAAGCGGAAGGAACATAGTGAGAAGAAGCAACGGGT CAGGTTCCAGCTTGAGATCTCTAGCAATCCAGAGGAGCAACGCAGCAGCATACTGCATTTGCAGGAGGCT GTGCGGCAGCATGTGGCCCAAATACGACAGCTGGAGAAGCAGATGTATTCTATCATGAAGTCTTTGCAGG ATGACAGCAAGAATGAGAATCTGCTTGACCTGAACCAGAAGCTTCAGTTGCAGCTAAACCAGGAGAAGGC CAACCTGCAGCTGGAAAGTGAAGAACTGAATATCCTCATCAGGTGTTTTAAGGATTTCCAACTGCAGCGG GCTAACAAGATGGAGCTGCGAAAGCAGCAAGAAGATGTGAGTGTGGTCCGTCGCACTGAGTTTTACTTTG CTCAGGCACGGTGGCGCCTGACAGAGGAAGATGGACAGCTGGGAATTGCTGAATTAGAACTGCAGAGGTT CCTCTACAGCAAGGTGAATAAGTCTGATGACACAGCAGAACATCTTCTGGAGTTGGGCTGGTTTACCATG AACAACCTCCTCCCCAATGCTGTCTATAAGGTAGTACTGCGGCCCCAGAGCTCCTGCCAGTCTGGGCGAC AGCTAGCTCTCCGCCTCTTCAGCAAAGTTCGGCCCCCTGTTGGGGGTATCTCTGTTAAGGAGCATTTTGA GGTAAATGTGGTGCCTCTCACCATCCAGCTGACACACCAGTTCTTCCACAGAATGATGGGCTTTTTCTTT CCTGGCCGAAGTGTGGAAGATGATGAAGTTGGTGATGAAGAGGATAAGTCCAAACTGGTGACTACTGGAA TACCAGTGGTGAAGCCTCGGCAGCTGATTGCAACAGATGATGCAGTACCACTGGGCCCTGGGAAGGGTGT GGCACAGGGTTTGACTCGGAGTTCTGGGGTCAGAAGGTCATTTCGCAAATCGCCAGAGCACCCTGTGGAT GACATTGACAAGATGAAAGAGCGAGCTGCCATGAACAACTCCTTCATCTACATAAAGATTCCACAGGTTC CACTGTGTGTCAGCTACAAGGGTGAGAAGAACAGTGTGGACTGGGGTGACCTTAACCTGGTGCTGCCCTG TCTGGAGTACCACAACAACACATGGACATGGCTAGACTTTGCCATGGCTGTCAAAAGGGACAGCCGCAAA GCCCTGGTTGCCCAGGTAATCAAAGAGAAGCTAAGGCTGAAGTCTGCAACAGGCTCTGAGGTCCGGGGAA AGCTAGAAACTAAATCGGACCTGAACATGCAACAGCAGGAAGAGGAGGAGAAAGCCCGGCTCCTCATTGG TTTAAGTGTGGGCGACAAGAACCCTGGCAAGAAGTCCATCTTTGGCAGGCGCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223287 representing NM_014680
Red=Cloning site Green=Tags(s) MPLFFSALLVLLLVALSALFLGRWLVVRLATKWCQRKLQAELKIGSFRFFWIQNVSLKFQQHQQTVEIDN LWISSKLLSHDLPHYVALCFGEVRIRTDLQKVSDLSAPFSQSAGVDQKELSFSPSLLKIFCQLFSIHVDA INIMVLKVDTSESLWHIQISRSRFLLDSDGKRLICEVSLCKINSKVLKSGQLEDTCLVELSLALDLCLKV GISSRHLTAITVDVWTLHAELHEGLFQSQLLCQGPSLASKPVPCSEVTENLVEPTLPGLFLLQQLPDQVK VKMENTSVVLSMNSQKRHLTWTLKLLQFLYHRDEDQLPLRSFTANSDMAQMSTELLLEDGLLLSQSRQRI VCLNSLKASVQVTTIDLSASLVLNTCIIHYRHQEFSHWLHLLALETQGSSSPVLKQRKKRTFPQILAPII FSTSISNVNISIQLGDTPPFALGFNSISLDYQHLRPQSIHQRGVLTVDHLCWRVGSDSHIQRAPHPPNMH VWGEALVLDSFTLQGSYNQPLGLSSTQSDTLFLDCTIRGLQVEASDTCAQCLSRILSLMGPQSGKSAVSR HSSFGESVSLLWKVDLKVEDMNLFTLSALVGASEVRLDTLTILGSAETSTVGIQGLVLALVKSVTEKMQP CCKAPDIPTPVLSLSMLSITYHSSIRSLEVQCGAGLTLLWSPPDHMYLYQHVLATLQCRDLLRATVFPET VPSLALETSGTTSELEGRAPEPLPPKRLLNLTLEVSTAKLTAFVAEDKFITLAAESVSLSRHGGSLQAYC PELAAGFDGNSIFNFKEVEVQLLPELEEMILHRNPFPALQTLRNRVWLLSFGSVSVEFPYQYDFSRTLDE AVGVQKWLKGLHQGTRAWASPSPVPLPPDLLLKVEHFSWVFLDDVFEVKLHDNYELMKDESKESAKRLQL LDAKVAALRKQHGELLPARKIEELYASLERKNIEIYIQRSRRLYGNTPMRRALLTWSLAGLELVALADAS FHGPEHVVEQVQELDPGSPFPPEGLDLVIQWCRMLKCNVKSFLVRIRDYPRYLFEIRDWRLMGRLVGTEQ SGQPCSRRRQILHLGLPWGNVAVERNMPPLKFYHDFHSEIFQYTVVWGPCWDPAWTLIGQCVDLLTKPSA DPSPPLPWWDKSRLLFHGDWHMDIEQANLHQLATEDPYNTTENMHWEWSHLSFHWKPGQFVFKGDLDINV RTASKYDDCCFLHLPDLCMTLDLQWLCHGNPHDHHSVTLRAPEFLPEVPLGQLHDSYRAFRSENLNLSIK MDLTRHSGTISQPRILLYSSTLRWMQNFWATWTSVTRPICRGKLFNNLKPSKKKLGQHYKQLSYTALFPQ LQVHYWASFAQQRGIQIECSQGHVFTRGTQRLIPQAGTVMRRLISDWSVTQMVSDLSQVTVHLMASPTEE NADHCLDPLVTKTHLLSLSSLTYQRHSNRTAEEELSARDGDPTFHTHQLHLVDLRISWTTTNRDIAFGLY DGYKKAAVLKRNLSTEALKGLKIDPQMPAKKPKRGVPTSASAPPRVNTPSFSGQPDKGSSGGAYMLQKLI EETDRFVVFTEEESGMSDQLCGIAACQTDDIYNRNCLIELVNCQMVLRGAETEGCVIVSAAKAQLLQCQH HPAWYGDTLKQKTSWTCLLDGMQYFATTESSPTEQDGRQLWLEVKNIEEHRQRSLDSVQELMESGQAVGG MVTTTTDWNQPAEAQQAQQVQRIISRCNCRMYYISYSHDIDPELATQIKPPEVLENQEKEDLLKKQEGAV DTFTLIHHELEISTNPAQYAMILDIVNNLLLHVEPKRKEHSEKKQRVRFQLEISSNPEEQRSSILHLQEA VRQHVAQIRQLEKQMYSIMKSLQDDSKNENLLDLNQKLQLQLNQEKANLQLESEELNILIRCFKDFQLQR ANKMELRKQQEDVSVVRRTEFYFAQARWRLTEEDGQLGIAELELQRFLYSKVNKSDDTAEHLLELGWFTM NNLLPNAVYKVVLRPQSSCQSGRQLALRLFSKVRPPVGGISVKEHFEVNVVPLTIQLTHQFFHRMMGFFF PGRSVEDDEVGDEEDKSKLVTTGIPVVKPRQLIATDDAVPLGPGKGVAQGLTRSSGVRRSFRKSPEHPVD DIDKMKERAAMNNSFIYIKIPQVPLCVSYKGEKNSVDWGDLNLVLPCLEYHNNTWTWLDFAMAVKRDSRK ALVAQVIKEKLRLKSATGSEVRGKLETKSDLNMQQQEEEEKARLLIGLSVGDKNPGKKSIFGRRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014680 |
| ORF Size | 6705 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014680.5 |
| RefSeq Size | 7456 bp |
| RefSeq ORF | 6708 bp |
| Locus ID | 9703 |
| UniProt ID | Q14667 |
| Cytogenetics | 17q11.2 |
| Protein Families | Transmembrane |
| MW | 253.5 kDa |
| Gene Summary | This gene was initially characterized in human as having high expression levels in breast carcinomas and breast cancer cell lines. This gene also has increased expression in prostrate cancer cells relative to normal prostrate tissues. Expression of this gene is negatively regulated by direct binding of the microRNA miR-195 to its 3' UTR. miR-195 has been shown to modulate the invasiveness of prostrate cancer cells and xenograft metastases by downgrading expression of this gene. In mouse, the protein encoded by this gene was identified as an antigen on acute monocytic leukemia cells. In human, alternative splicing results in multiple transcript variants encoding distinct isoforms; some of these isoforms are predicted to contain an RNA pol II promoter FMP27 protein domain and a Golgi-body-localization APT1 domain. [provided by RefSeq, Apr 2017] |
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