DMRT2 (NM_181872) Human Tagged ORF Clone

CAT#: RC223278

DMRT2 (Myc-DDK-tagged)-Human doublesex and mab-3 related transcription factor 2 (DMRT2), transcript variant 3

Product Images

Circular map for RC223278

• 

  Western blot validation of overexpression lysate (Cat# LY405565) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223278 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DMRT2
• Synonyms: DSXL-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223278 representing NM_181872
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACCCGCAGGCTGGCTCCGCGGCCGGGGACTGGGAGATCGATGTCGAGAGCCTGGAGCTGGAAG
  AGGACGTCTGCGGGGCGCCGCGGTCCACGCCCCCCGGGCCCAGCCCGCCGCCGGCGGACGGGGACTGCGA
  GGACGACGAAGATGACGACGGGGTGGACGAAGACGCGGAAGAAGAGGGCGACGGCGAGGAGGCAGGCGCG
  TCCCCCGGGATGCCCGGCCAGCCGGAGCAGCGGGGGGGACCGCAGCCGAGGCCGCCGCTCGCGCCTCAGG
  CCTCACCCGCCGGCACCGGTCCCCGAGAGCGCTGCACTCCCGCGGGCGGCGGCGCGGAGCCGCGCAAGCT
  GAGCCGCACGCCCAAGTGCGCGCGCTGCCGCAACCACGGCGTGGTGTCCTGCCTGAAGGGCCACAAGCGC
  TTCTGTCGCTGGCGCGACTGCCAGTGCGCCAACTGCCTGCTGGTGGTGGAGCGGCAGCGCGTCATGGCCG
  CCCAGGTGGCGCTCCGGAGGCAGCAGGCCACCGAGGACAAGAAGGGGCTTTCCGGGAAACAGAATAATTT
  CGAGCGCAAAGCTGTGTACCAGAGGCAAGTCAGAGCCCCCAGTTTGCTGGCCAAAAGCATTTTAGAAGGC
  TATCGCCCCATTCCAGCGGAGACTTATGTAGGAGGGACCTTCCCTCTACCTCCCCCAGTTAGTGACAGGA
  TGAGGAAAAGAAGAGCCTTTGCTGATAAAGAGTTGGAGAACATTATGCTGGAGAGAGAATATAAAGAAAG
  GGAGATGTTGGAAACTTCTCAAGCTGCTGCTCTGTTTCTGCCCAACCGCATGGTGCCTGGACCTGACTAC
  AATTCCTACAAAAGTGCCTACAGCCCCAGCCCAGTGGAACCACCAAGCAAGGACTTCTGTAATTTTTTGC
  CCACCTGCCTTGATTTAACCATGCAGTATTCAGGGTCTGGGAATATGGAACTAATTTCTTCTAATGTCAG
  CGTGGGCACAACTTATAGACAGTATCCCTTGTCCTCAAGATTTTTAGTTTGGCCCAAGTGTGGCCCCATT
  AGCGACACCCTCCTCTACCAGCAATGCCTGCTAAATGCCACCACCTCAGTTCAAGCCCTGAAGCATGGGG
  CCAGCTGGGACTTGAAGGGAGCACGAGTCCAGGATGGACTCAGTGCAGAGCAGGACATGATGCCATCGAA
  ATTGGAAGGTTCCCTGGTGCTGCCTCACACTCCTGAGATCCAGACCACGAGAAGTGACCTTCAGGGTCAT
  CAGGCTGTCCCAGAGAGGTCCGCGTTCTCCCCACCCCGACGGAATTTCTCTCCCATTGTTGACACGGACT
  CCCTGGCAGCTCAAGGGCATGTCTTAACGAAGATCAGCAAAGAAAACACCAGGCACCCTCTGCCACTTAG
  ACATAATCCATTCCACTCATTATTCCAGCAAACACTTACTGACAAATCGGGTCCTGAGTTGAAAACACCA
  TTTGTCAAAGAGGCCTTTGAAGAGACCCCTAAGAAACACAGAGAGTGTTTAGTTAAGGACAACCAGAAGT
  ACACATTTACAATAGATAGATGTGCAAAAGACCTTTTTGTAGCCAAACAAGTTGGAACAAAACTCTCGGT
  GAATGAACCACTGTCATTTTCTGTTGAGTCTATTCTTAAGAGGCCTTCATCTGCCATCACTCGTGTCTCT
  CAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223278 representing NM_181872
  Red=Cloning site Green=Tags(s)
  
  MADPQAGSAAGDWEIDVESLELEEDVCGAPRSTPPGPSPPPADGDCEDDEDDDGVDEDAEEEGDGEEAGA
  SPGMPGQPEQRGGPQPRPPLAPQASPAGTGPRERCTPAGGGAEPRKLSRTPKCARCRNHGVVSCLKGHKR
  FCRWRDCQCANCLLVVERQRVMAAQVALRRQQATEDKKGLSGKQNNFERKAVYQRQVRAPSLLAKSILEG
  YRPIPAETYVGGTFPLPPPVSDRMRKRRAFADKELENIMLEREYKEREMLETSQAAALFLPNRMVPGPDY
  NSYKSAYSPSPVEPPSKDFCNFLPTCLDLTMQYSGSGNMELISSNVSVGTTYRQYPLSSRFLVWPKCGPI
  SDTLLYQQCLLNATTSVQALKHGASWDLKGARVQDGLSAEQDMMPSKLEGSLVLPHTPEIQTTRSDLQGH
  QAVPERSAFSPPRRNFSPIVDTDSLAAQGHVLTKISKENTRHPLPLRHNPFHSLFQQTLTDKSGPELKTP
  FVKEAFEETPKKHRECLVKDNQKYTFTIDRCAKDLFVAKQVGTKLSVNEPLSFSVESILKRPSSAITRVS
  Q
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181872
• ORF Size: 1683 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181872.1, NP_870987.1
• RefSeq Size: 1969 bp
• RefSeq ORF: 1686 bp
• Locus ID: 10655
• UniProt ID: Q9Y5R5
• Cytogenetics: 9p24.3
• Protein Families: Transcription Factors, Transmembrane
• MW: 61.6 kDa
• Gene Summary: The protein encoded by this gene belongs to the DMRT gene family, sharing a DM DNA-binding domain with Drosophila 'doublesex' (dsx) and C. elegans mab3, genes involved in sex determination in these organisms. Also, this gene is located in a region of the human genome (chromosome 9p24.3) associated with gonadal dysgenesis and XY sex reversal. Hence this gene is one of the candidates for sex-determining gene(s) on chr 9. [provided by RefSeq, Apr 2010]