KIAA1614 (NM_020950) Human Tagged ORF Clone

CAT#: RC223211

KIAA1614 (Myc-DDK-tagged)-Human KIAA1614 (KIAA1614)

Product Images

Circular map for RC223211

• 

  Western blot validation of overexpression lysate (Cat# LY412175) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223211 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KIAA1614
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223211 representing NM_020950
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGGACAGAGGCGGCGGCGGCCAAACCCGCGGGCGGCAGCCCCCAAGGGCCCAAGACAGGGAGTG
  GAACAGCCAGCCCCGTGGAGGGGACCTCAGCTGTGGAGTGGAGTGGTCCTGAGCCACAGCTGGATAACGG
  ACACCCCCCAAGACCCTGGCCTTGCCCTCAGGAAAACAGAACATCCAGCCTGATGGCCCCCCAGCCTCCC
  AGGGTATGGGGAGTACAGCTCCAGGGCCCCTCTGTGCTGGAATCCAAGGTGAGGGCTTTGAAGGAGAAGA
  TGACAGTGGCCAAACAGGGAGTGAGTCCCTGCTCTGCTTCCCAAGAGTGGTCATCCCCCAAGAAACCCCA
  ATGCAGACGAGGCAAGGCAGGGAGAGCCGGGACTCCATCAGAGGGGTCTTTCCTGCCAGGTGCTGTGGTG
  GCTCCTCGTACCCAAAACCTGCCTGATGGGCAGCTGGACGGCAGCATCAATGAGGAGCAACCCGCCAGGG
  ATGGAGGCCCCAGGCTTCCCAGGCCGCCTGCCCCTGGACGTGAGTACTGCAACAGGGGGAGCCCGTGGCC
  TCCAGAAGCCGAATGGACACTTCCTGACCATGACAGAGGTCCGCTGCTGGGGCCCAGCTCTTTGCAACAG
  AGCCCGATCCATGGAGTTACTCCCGGACGGCCTGGGGGTCCTGGTCATTGTAACAAAATCATCCACATTC
  CCAGCCCAAGGACAGGAAGGTCCTACCCTTTTCCAGATGGCGTGGTGACAGAGGCAGATCTGGATAGCAC
  ATCCCTGACCTCCGAGGAGGTCTTTGTCCCCAGGACGGCCCTGCTGGGTGAGCGCTGGAGAGCTGGAGAC
  CTGGAGGCTCTGGGCGCTGGGAGCAGTGTCTTGTCCCTGTCTGATCGGGTGGAGAGAAACCGCCTGTTGC
  TGCAGGAGATGCTCAACGTTTCTGGGCAGAGCCCCCGCAAGGTGGGAACCCCTGCCTGGACTCCATCCTG
  GGACACAGCTGCACCAGAGCGACCAGTGGGGGATGTGGACTGGGCCTCGGGCACCTCCTTGCAGGACTCC
  GGCCAGAACAGGACCGTTGGTCCCAACCCGGAGCCTGTGCTGAGCCCCAGGCATGAGGAAGCCACGCATC
  TGCTGCAGCGTGCCCGCATGAAGGCCAGGACCCGGCCCCTCCGTGCCAGCCATGACATCGTGCCCACCAT
  TACCCAGGGCAGCCGAGATGGCCACAGAAGCCCAGCCCGGGACCCCAGGACGACCCCTGCCTGCAGAGAC
  AGCCTCCAGAACGGGCACACGAGCGATTCCTCCAGCGGAGAGTCCAGCGGTGGGCACAGGCCGAGGCGGG
  GCCCCTCGCCGTCGCACGTGCGCTTTGAGGATGAGTCCGCCCGCGAAGCCGAGTTCCGTCACCTGGAGCG
  GCTGCAGCAGCGCCAGCGCCAGGTGCTGAGCACCGTGTTGCAGGCCGCGGACCAGGGCCCCCTGCGCTCC
  AAGCCCGACCTCGCCGACTACATCAACGGGGCTCCCCGGCTCCGGGACGCGGGGCAGGGGACATTCCACA
  GGCTTGTGGGCAGCCTGGACCGCAGGGGACACCCGGCACCGCCGGCACCGGGCAGCGAGAGGAGGTGCCA
  GGCCTGCGGCAGCTGCATCGACGACCCGCGCCCCGCCCAGGGGAAGGCGCCCCCCGTCCCCAGGACCCTC
  CAGGAGCTCCAGGCTGCCTGTGGGATGGAGAGGGTGCTGGGTGGCCTGAGCTCCCCACTCCGGCTCCTTC
  CTGCAGAGCCCCGGCTCCACATGGAATGGATCCGGGAAACACACATCGGAGACACCGTGTGCCCTGCGGA
  GGTGGACTCTGCCCTGGACAGCACAGACAACTCTGACAACTGCAGGACCGACAGTGAGGAGGCGGGGACC
  TCTCAGGCTGGCTGGGCGTGTGGGCGGACCCAAGGCAGCAGCCCGCGACTGCGACTGCGGGGCTCCAGGC
  CTCGAGGCCACAGGTGGTCCAAGAAGGCTGAGGCGGAGCTCCCTTGGGGCCTTCAGGCCCAGCAACACCT
  GCCTAGGGCTGATGATGTGGAGGTGGAAAATGAGGTGAAAGAGGGCAGAGGACACACGCCTGAAGGAACT
  CTATTTTTGAGAGAAGATGCCAAGCCTCCTGACCTGGAGTTGAAGCGGGTGTCCCTGGGACCCCAGTGGC
  AGCCTGGACCAGGGCTGGGAAGTCACCAGCCTCACCCTTTGGATTCCCGGACTCCATGCAGGACAGCCTA
  TGCCACCACCGCCCCCATGACGCCTGAATCATCGGGGCCAGGAGGCCAGGCCCAGGTTACAGAAAGCCAC
  GAGTCCCTGGAAATTGTCTCTCCTTCCTCCCTGCAACAGAGCCATGCAGAGCCTTCTGCCCCACACCAAG
  CCTGGCAGCCAACAGCTTCCTTGTGTCCTGAAGGCTGGGCGCCAACCCCTCCCCCTTCGAGGAAAACCAC
  CTCGCCAGTGTCTCACAGGAAGGCAGCCCTGGCTGGACTGCTCAGGCTGGGTGACCAGACAGAGCCTGTG
  GGTATCCCTCGGCCTCCTTCAAGAAGCGCGGTTCTCAGGACCTGTGAGCTGCCCCCATCACAGACCCAGC
  CCAGCCGCCCTCAGGTCAGGCACCCACTGCTGGCCCTGTCCACCAACAACTGCAACAACAGCGCACCTCG
  GGGGCTGCAGGAGCCCTACGGGGGAGCCGTCCACGAGGGTAGGGTGGAGAGGGGCCCCTGCAGCCGGGAA
  CCGGAGCCGCCCCTGGAGAACAGCAGAGATGGAGGACCCCAGGGCTTTCTTGGCTCAGCAGATGTTGCCA
  CCATCAACTCCACGGGCATCACCCTCTCCCTGTCCTCAGAGGAGTCAGAGTCCAGCAAGGAATCAGAGGG
  AAGCCTGCAGAGGACAGGGTCAGGATCTGGAGGACATGTGCTGTCAAGAGCATCAGCAGGAGCTGGCACA
  GGACCCGGCTCCCCCTCGGCTGCCCCTTTGGACCAGAACAAGAAAAGGAGCAGCAGCATAGCCTCCACCC
  TGGGGCTGAAAAAGCTCTTCTCAGCCCTGGGCCAGAGTTCCCGGCCCAAGCTGGGCAAGTCCCGCAGCTA
  CAGTGTGGAGCAGTTGCAGCCCGCCCCGCCTGGCCTGACGTCACAGTCCAGGGCCCCATCGTTACAATCC
  CTGCACCCGGTGTCACCCTCTCACCAGCGTCGGAAAGCTGCCTCTTTTCAGAACCTCCATTCTCTGCTGA
  GCAGCAAGGGGAACCGGTCCAGCCTCTACCTGGTAGCAGGGCCAGGGGACCACAGTGCAGCTGGCAGGCC
  GGCCAAGACTTCACCACGGCGTGCCCTCAGTGTGGAGGACGTGGGTGCTCCCAGCCTGGCTCGCACCGTG
  GGCCGCCTGGTGGAGGTGTTCCCAGACGGCACCAGCCAGCTGCAGCTGCAGCGCTCCCCAGGGGGCACTT
  TCGGCTTCTGCGTGGCCTCTGGGAATGGGCGCCCAGACTCAGGTATGCCCTCTCCTCTTCCTCAGCCCCA
  TGGCTGGGGCGGCCTTAGCAAACAAGGCAGGGCCTTCTGGCTGTGGTCAGAGGCTTTTCTGGTCTTTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223211 representing NM_020950
  Red=Cloning site Green=Tags(s)
  
  MEGTEAAAAKPAGGSPQGPKTGSGTASPVEGTSAVEWSGPEPQLDNGHPPRPWPCPQENRTSSLMAPQPP
  RVWGVQLQGPSVLESKVRALKEKMTVAKQGVSPCSASQEWSSPKKPQCRRGKAGRAGTPSEGSFLPGAVV
  APRTQNLPDGQLDGSINEEQPARDGGPRLPRPPAPGREYCNRGSPWPPEAEWTLPDHDRGPLLGPSSLQQ
  SPIHGVTPGRPGGPGHCNKIIHIPSPRTGRSYPFPDGVVTEADLDSTSLTSEEVFVPRTALLGERWRAGD
  LEALGAGSSVLSLSDRVERNRLLLQEMLNVSGQSPRKVGTPAWTPSWDTAAPERPVGDVDWASGTSLQDS
  GQNRTVGPNPEPVLSPRHEEATHLLQRARMKARTRPLRASHDIVPTITQGSRDGHRSPARDPRTTPACRD
  SLQNGHTSDSSSGESSGGHRPRRGPSPSHVRFEDESAREAEFRHLERLQQRQRQVLSTVLQAADQGPLRS
  KPDLADYINGAPRLRDAGQGTFHRLVGSLDRRGHPAPPAPGSERRCQACGSCIDDPRPAQGKAPPVPRTL
  QELQAACGMERVLGGLSSPLRLLPAEPRLHMEWIRETHIGDTVCPAEVDSALDSTDNSDNCRTDSEEAGT
  SQAGWACGRTQGSSPRLRLRGSRPRGHRWSKKAEAELPWGLQAQQHLPRADDVEVENEVKEGRGHTPEGT
  LFLREDAKPPDLELKRVSLGPQWQPGPGLGSHQPHPLDSRTPCRTAYATTAPMTPESSGPGGQAQVTESH
  ESLEIVSPSSLQQSHAEPSAPHQAWQPTASLCPEGWAPTPPPSRKTTSPVSHRKAALAGLLRLGDQTEPV
  GIPRPPSRSAVLRTCELPPSQTQPSRPQVRHPLLALSTNNCNNSAPRGLQEPYGGAVHEGRVERGPCSRE
  PEPPLENSRDGGPQGFLGSADVATINSTGITLSLSSEESESSKESEGSLQRTGSGSGGHVLSRASAGAGT
  GPGSPSAAPLDQNKKRSSSIASTLGLKKLFSALGQSSRPKLGKSRSYSVEQLQPAPPGLTSQSRAPSLQS
  LHPVSPSHQRRKAASFQNLHSLLSSKGNRSSLYLVAGPGDHSAAGRPAKTSPRRALSVEDVGAPSLARTV
  GRLVEVFPDGTSQLQLQRSPGGTFGFCVASGNGRPDSGMPSPLPQPHGWGGLSKQGRAFWLWSEAFLVFG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020950
• ORF Size: 3570 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020950.2
• RefSeq Size: 4155 bp
• RefSeq ORF: 3573 bp
• Locus ID: 57710
• UniProt ID: Q5VZ46
• Cytogenetics: 1q25.3
• MW: 126.4 kDa